16S V7-V9 Library Preparation Kit for Illumina

Cat. 70900, 70910, 70920, 70930, 70940
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16S V7-V9 Library Preparation Kit for Illumina

Cat. 70900, 70910, 70920, 70930, 70940

The reagents and components required for library preparation of the 16S V7-V9 amplicon libraries to be used for next-generation sequencing on Illumina platforms

  • Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
  • Simple and quick workflow: library could be prepared in less than 5 hours
  • Component of Norgen's metagenomics workflow
  • A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

For research use only and NOT intended for in vitro diagnostics.

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16S V7-V9 Library Preparation Kit for Illumina
(Cat. 70900)
24 Preps
16S V7-V9 Library Preparation Kit for Illumina
(Cat. 70910)
96 Preps Set A
16S V7-V9 Library Preparation Kit for Illumina
(Cat. 70920)
96 Preps Set B
16S V7-V9 Library Preparation Kit for Illumina
(Cat. 70930)
96 Preps Set C
16S V7-V9 Library Preparation Kit for Illumina
(Cat. 70940)
96 Preps Set D

Product Size

Kit Specifications

You have selected: Cat. 70900
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70910
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70920
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70930
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70940
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Supporting Data

Click for expanded view

Title Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis
Journal Journal of Veterinary Internal Medicine. 2022.
Authors Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow

16S V7-V9 Library Preparation Kit for Illumina

The 16S V7-V9 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V7-V9 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V7-V9 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V7-V9 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

16S Library Prep

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 70900
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70910
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70920
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70930
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

You have selected: Cat. 70940
Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL)
Time to complete library preparation: 4 hours

Storage Conditions and Product Stability
Norgen’s 16S V7-V9 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Title Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis
Journal Journal of Veterinary Internal Medicine. 2022.
Authors Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow

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