ProteoSpin™ Urine Protein Concentration Kits
For the rapid and efficient concentration of total proteins from urine samples
For research use only and NOT intended for in vitro diagnostics.
ProteoSpin™ Urine Protein Concentration Kits
For the rapid and efficient concentration of total proteins from urine samples
Register today to receive an exclusive 15% off* on your first order.
Features and Benefits
- Simultaneous concentration, desalting and buffer exchange of total urinary proteins
- No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.
ProteoSpin™ Urine Protein Concentration Micro Kit
Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.
ProteoSpin™ Urine Protein Concentration Midi Kit
The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.
ProteoSpin™ Urine Protein Concentration Maxi Kit
The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.
Details
Supporting Data
Kit Specifications
|
|
Maximum Urine Input Volume |
20 mL
|
Maximum Recovered Protein |
4 mg
|
Minimum Elution Volume |
2 mL
|
Time to Process 4 Samples |
45 minutes
|
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Component | Cat. 17400 (25 preps) | Cat. 52300 (10 preps) | Cat. 21600 (4 preps) |
---|---|---|---|
Wash Solution C | 60 mL | 60 mL | 130 mL |
Binding Buffer A | 4 mL | 4 mL | 8 mL |
Elution Buffer C | 8 mL | 30 mL | 30 mL |
Protein Neutralizer | 4 mL | 4 mL | 4 mL |
Micro Spin Columns | 25 | - | - |
Midi Spin Columns (assembled with collection tubes) | - | 10 | - |
Maxi Spin Columns (assembled with collection tubes) | - | - | 4 |
Collection Tubes | 25 | - | - |
Elution Tubes (1.7 mL) | 25 | - | - |
Midi Elution Tubes | - | 10 | - |
Elution Tubes (50 mL) | - | - | 4 |
Product Insert | 1 | 1 | 1 |
Documentation
FAQs
Micro, Midi, Maxi
-
Centrifugation speed was too low.
Check the centrifuge to ensure that it is capable of generating 3,300 x g (micro kit), or 1,000 x g (midi and kits). Sufficient centrifugal force is required to move the liquid phase through the resin. Centrifugation speeds may be increased to 6,700 x g (micro kit), or 2,000 x g (midi and maxi kits), but this speed should not be exceeded.
-
Inadequate spin time.
Spin additional 1 minute (micro kit), or 3 minutes (midi and maxi kits) to ensure that the liquid is able to flow completely through the column.
- Incorrect pH adjustment of urine sample.
Depending on a person’s acid-base status, the starting pH of the urine may range from 4.5 to 8. Therefore, it is important that the proper amount of Binding Buffer A be added to the urine sample in order to adjust the pH to 3.5 - 4 prior to loading onto the column. Especially with large volumes for midi and maxi kits, it is important to verify this and if necessary, add more Binding Buffer A to adjust the sample to pH 3.5 - 4.
- Initial volume of sample applied to column was too low.
Ensure that 1 mL (micro kit), or 5 mL (midi kit), or 20 mL (maxi kit) of the pH-adjusted urine sample is loaded onto the column in order to capture a large portion of the proteins present in the sample.
- Eluted protein solution was not neutralized.
Please refer to individual product manual for correct volume of Protein Neutralizer to be used in order to adjust the pH to neutral. Some proteins are sensitive to high pH, such as the Elution Buffer C at pH 12.5.
- Eluted protein solution was not neutralized quickly enough.
If eluted protein is not neutralized immediately, degradation will occur. We strongly suggest adding Protein Neutralizer to lower the pH.
Citations
Title | KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine |
Citation | European Journal of Pharmaceutical Sciences 2016. |
Authors | Puhka, M., Nordberg, M. E., Valkonen, S., Rannikko, A., Kallioniemi, O., Siljander, P., & af Hällström, T. |
Title | Mass spectrometric quantification of urinary human liver fatty acid binding protein in renal transplant recipients |
Citation | Rapid Communications in Mass Spectrometry 2016. |
Authors | Filiz Ozcan, Halide Akbas, Ebru Kirac, Gultekin Suleymanlar, Mutay Aslan, Gultekin Yucel |
Title | Comparison of Depletion Strategies for the Enrichment of Low-Abundance Proteins in Urine |
Citation | PLoS One 2015. |
Authors | S Filip, K Vougas, J Zoidakis, A Latosinska, W Mullen, G Spasovski, H Mischak, A Vlahou, J Jankowski |
Title | Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins |
Citation | Journal of Proteomics 2015. |
Authors | S Bathia, P Rawat, R Baithalu, ML Yadav, J Naru, A Tiwari, S Kumar, AK Balhara, S Singh, S Chaudhary, R Kumar, M Lotfan, P Behare, SK Phulia, TK Mohanty, JK Kaushik, S Nalapeta, I Singh, SK Ambatipudi, AK Mohanty |
Title | Optimization for peptide sample preparation for urine peptidomics |
Citation | Clinical Proteomics 2014. |
Authors | TK Sidgel, CD Nicora, SC Hseih, WJ Qian, DG Camp II, MM Sarwal |
Title | Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract |
Citation | Kidney International 2011. |
Authors | John D. Spencer1,2,8, Andrew L. Schwaderer2,8, Julianne D. DiRosario3, Kirk M. McHugh4, Glen McGillivary5, Sheryl S. Justice5, Ashley R. Carpenter4, Peter B. Baker6, Ju¨rgen Harder7 and David S. Hains2,3,8 |
Title | Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS |
Citation | Proteomics Clinical Applications 2007, 1, 577-587. 2007. |
Authors | Yi-Ting Chen, Chao-Yun Tsao, Jen-Ming Li, Chih-Yen Tsai, Su-Feng Chiu, Tzu-Ling Tseng, Dr. |