Total RNA Purification Kits Dx For the isolation and purification of total RNA from tissue samples, blood, plasma, serum, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use Note: This product is not available for sale in the United States Powered by Bioz Total RNA Purification Kits Dx For the isolation and purification of total RNA from tissue samples, blood, plasma, serum, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use Price USD$289.00 SKU Dx17200 Format Spin Column High Throughput High Throughput Deep Well Size 50 Preps Quantity Overview Details Documentation Citations Overview CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746. Available for sale in Canada (Dx17200, Dx24300) Ideal for use in in vitro diagnostic workflows Extract high quality & quantity total RNA including miRNA No phenol step required Bind & elute all RNA irrespective of size or GC content, without bias Very sensitive & linear down to a few cells without the need for carrier RNA Convenient & fast spin column format Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation Isolate from a wide variety of specimens Purified RNA is suitable for a variety of downstream applications Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. Total RNA Purification Kit Dx (Spin Column) This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well) Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses. Intended Use To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay. NOTE: This product is not available for sale in the United States. Details Supporting Data Previous Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit Dx allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgen’s Total RNA Purification Kit and a competitor’s kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation. Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction. Norgen's Total RNA Purification Kit Dx isolates the complete size range of RNA, including small RNAs without the use of phenol. Total RNA was isolated from 0.75 million HeLa cells using Norgen's Total RNA Purification Kit Dx, various silica-based competitors and phenol-based TRI Reagent. RT-PCR was performed according to Shi and Chiang (2005). Fifteen microliters of the 50 µL isolated RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was used in a 20 µL qPCR reaction with primers against the human microRNAs (miR-19 and miR-21) and large mRNA (S15). Only the use of Norgen's Total RNA Purification Kit Dx resulted in detection of both microRNAs and mRNA amplification products similar to Competitor 2's kit but without the use of phenol. Figure 3. High Quality of RNA from a Diverse Range of Inputs. Norgen's Total RNA Purification Kit Dx allows RNA isolation from a wide range of species or tissue types. Total RNA was isolated from 5 x 108 E. coli cells, 1 x 106 HeLa cells, 100 µL rat blood and 10 mg hamster kidney using Norgen's Total RNA Purification Kit Dx. One microliter of the 50 µL isolated RNA was analyzed on the Agilent® 2100 BioAnalyzer using an RNA Nano 6000 chip. Note the integrity of RNA from all inputs with the presence of small RNA species. Norgen's Total RNA Purification Kit Dx consistently isolates high quality RNA from various inputs that score a RIN value between 8 and 10. Figure 4. Great Isolation Sensitivity. Norgen's Total RNA Purification Kit Dx allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of 293 HEK cells from 1 million cells down to a single cell. Five microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL PCR reaction with primers to detect the human beta-actin transcripts. PCR products of beta-actin were detected from as little as a single cell. M is the marker lane. Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA. Norgen’s Total RNA Purification Kit Dx allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit Dx (blue), a competitor’s silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification Dx had the best linearity (higher R2) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21). Figure 6. Effective and Consistent Detection of miRNA from Plasma. Norgen's Total RNA Purification Kit Dx can effectively isolate miRNA from plasma. Total RNA was isolated from 50, 100 or 200 µL of rat plasma in triplicates using Norgen's Total RNA Purification Kit Dx (blue), a competitor’s silica-based kit (green) and a phenol-based RNA extraction method (red). Stem loop RT-qPCR using primers specific to miR-21 was performed. In brief, two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21. Norgen's Total RNA Purification Kit Dx is the only product that showed (1) consistent detection of miR-21 transcripts across all input volumes, and (2) Ct values correlated to input volume (decrease Ct with increase input). Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency. Norgen's Total RNA Purification Kit Dx isolates plasma RNA that performs more consistently in downstream applications such as microarrays. Total RNA including miRNA was isolated from 100 µL of mouse plasma in duplicate using Norgen's Total RNA Purification Kit Dx, Competitor 1's leading miRNA Kit or a phenol-based RNA extraction method. One hundred nanograms of extracted RNA from each kit was applied to an Illumina microRNA expression profiling kit. Scatter plots display better consistency (better clustering) of replicate signals from Norgen's samples. Genes with Pval < 0.01 for both replicates were in blue. The associated table suggested that Norgen's protocol recovered the same diversity of miRNA with better consistency (higher r2 value). Figure 8. Better Diversity of miRNA Detected from Plasma. Norgen's Total RNA Purification Kit Dx isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit Dx or 625 µL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit Dx (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitor’s miRNA kit (right). Image courtesy of LC Sciences, Houston. Figure 9. Better Diversity of miRNA Detected from HeLa Cells using Illumina Small RNA Next Gen Sequencing. Norgen's Total RNA Purification Kit Dx isolates miRNA from HeLa cells with better diversity than a leading competitor. Total RNA including miRNA was isolated from 1 million HeLa cells using Norgen's Total RNA Purification Kit Dx and Competitor Q's leading miRNA Kit, and was applied to Illumina Small RNA Next Gen Sequencing on a MiSeq sequencer. Panel A showed that Norgen’s Total RNA Purification Kit Dx recovers a higher number of miRNAs than the competitor. In particular, the higher diversity is achieved with a faster and simpler procedure in as little as 20 minutes of RNA sample preparation time without the use of phenol. Panel B is a scatter plot of the average RPM (reads per million) of the miRNAs detected used to compare Norgen and the competitor’s kit recovery of miRNA. Norgen’s Total RNA Purification Kit Dx recovered a significantly higher number of miRNAs that have higher RPM, as summarized in the graph insert as well as in Panel C. Figure 10. Consistent Yield of High Quality RNA with Complete Size Range without the Use of Phenol. Norgen's Total RNA Purification 96-Well Kit allows for the consistent isolation of high quality RNA, with complete sizes ranging from very large RNA down to small RNA without the use of phenol. Total RNA was isolated from 0.5 mL aliquots of an overnight culture of E. coli (~5 x 108 cells/mL) using Norgen's Total RNA Purification 96-Well Kit in 16 replicates. Five microliters of the 75 µL isolated RNA were resolved on a 1.2% formaldehyde agarose gel. All 16 replicates showed both high yield and high quality. In addition, all replicates showed effective recovery of all sizes of RNA including the small RNA (arrow). Figure 11. High Quality of RNA from a Diverse Range of Inputs. Norgen's Total RNA Purification 96-Well Kit allows for RNA isolation from a wide range of species and tissue types. Total RNA was isolated from 8 mg of brain (Lanes A), kidney (Lanes B), liver (Lanes C), lung (Lanes D) and spleen tissue (Lanes E), 7.5 x 105 HeLa (Lanes F) and CHO cells (Lanes G), and 5 x 108 bacteria (Lanes H) using Norgen's Total RNA Purification 96-Well Kit. Five microliters of the 75 µL isolated RNA was resolved on a 1.2% formaldehyde agarose gel. From observing the formaldehyde-agarose gel, it can be seen that Norgen's kit can be used to successfully isolate total RNA, including small RNA species, from a broad range of sample types. Figure 12. Isolation of Both Large and Small RNA. Norgen's Total RNA Purification 96-Well Kit allows for consistent isolation of both large and small RNA. Total RNA was isolated from samples of 5 x 105 HeLa cells using Norgen's Total RNA Purification 96-Well Kit. Aliquots of each total RNA sample were then used in 2 different RT-qPCR reactions. Ten microliters of the 75 µL isolated RNA was subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15 transcripts (Panel B), respectively. Both the mRNA and microRNA were amplified in a consistent manner from all the samples, with low variability of the Ct values. Thus both types of RNA are being consistently isolated using this kit. Figure 13. Great Isolation Sensitivity. Norgen's Total RNA Purification Kit allows for sensitive RNA extraction from even less than 10 cells. RT-qPCR was used to detect mRNA isolated from various input amounts of HeLa cells, from 100,000 down to a single cell, using Norgen's Total RNA Purification 96-Well Kit. Ten microliters of the 75 µL isolated RNA were then subjected to a 20 µL reverse transcription using oligo dT primers. Three microliters of the reverse transcription were used in a 20 µL real-time PCR reaction with primers to detect the human S15 transcripts. The resulting Ct values were plotted against the input cell number. Total RNA was isolated and detected linearly from as little as a single HeLa cell. Next Click for expanded view Kit Specifications Binding Capacity Per Column Up to 50 μg RNA Maximum Loading Volume Per Spin Column 650 μL Size of RNA Purified All sizes, including < 200 nt Time to Complete 10 Purifications 20 minutes Maximum Amount of Starting Material Tissues 10 mg (for most tissues) Blood 100 µL Plasma/Serum 200 µL Bacteria 1 x 109 cells Yeast 1 x 108 cells Fungi 50 mg Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels Component Cat. Dx17200 (50 preps) Cat. Dx24300 (192 preps) Cat. Dx24350 (192 preps) Cat. Dx24380 (576 preps) Lysis Solution 40 mL - - - Buffer RL - 2 x 40 mL 2 x 40 mL 350 mL Wash Solution 22 mL - - - Wash Solution A 22 mL 2 x 38 mL 2 x 38 mL 1 x 148 mL1 x 74 mL Elution Solution 6 mL - - - Elution Solution A - 2 x 20 mL 2 x 20 mL 60 mL Mini Spin Columns 50 - - - 96-Well Isolation Plate - 2 2 - 96-Well Isolation Plate (Deep Well) - - - 6 Collection Tubes 50 - - - 96-Well Collection Plate - 2 2 - 96-Well Collection Plate (Deep Well) - - - 6 Elution Tubes (1.7 mL) 50 - - - 96-Well Elution Plate - 2 2 - 96-Well Elution Plate (Deep Well) - - - 6 Adhesive Tape - 4 4 12 Product Insert 1 1 1 1 Documentation PROTOCOLS (Dx17200) Total RNA Purification Kit Dx (Spin Column) - Protocol (50 preps) (Dx24300) Total RNA Purification 96-Well Kit Dx (High Throughput) - Protocol ( 2 x 96-well plates) (Dx24350) Total RNA Purification 96-Well Kit Dx (High Throughput Deep Well) - Protocol (2 x 96-well plates) (Dx24380) Total RNA Purification 96-Well Kit Dx (High Throughput Deep Well) - Protocol (6 x 96-well plates) SUPPLEMENTARY PROTOCOLS Supplementary Protocol - Total RNA Isolation From Cerebrospinal Fluid Csf Supplementary Protocol - Norgen's Saliva RNA Collection and Preservation Devices Supplementary Protocol for Isolating Viral RNA from Swab Samples APPLICATION NOTES High-Throughput Purification of Total RNA Including miRNA without the use of Phenol Extraction Isolation and Detection of RNA from as little as a Single Animal Cell Using Norgen’s Total RNA Purification Kit SAFETY DATA SHEETS (24300) Total RNA Purification 96-Well Kit - Safety Data Sheet POSTERS Explore the World of RNA - Silicon-Carbide vs. Silica-min.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Spin Column (Dx17200) Title Analysis of plasma microRNA expression profiles in a Chinese population occupationally exposed to benzene and in a population with chronic benzene poisoning Journal Journal of Thoracic Disease. 2016. 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Authors Christine How, Jeff Bruce, Jonathan So, Melania Pintilie, Benjamin Haibe-Kains, Angela Hui, Blaise A Clarke, David W Hedley, Richard P Hill, Michael Milosevic, Anthony Fyles, Fei-Fei Liu Title Endogenous tRNA-Derived Fragments Suppress Breast Cancer Progression via YBX1 Displacement Journal Cell. 2015. Authors Hani Goodarzi, Xuhang Liu, Hoang C.B. Nguyen, Steven Zhang, Lisa Fish, Sohail F. Tavazoie Title Effects of Protein A in Detection of Canine Distemper Virus through immunosensor construction Journal IEEE Sensors Journal. 2015. Authors Caroline Rodrigues Basso, Juliane R. Sempionatto, Claudia C. Tozato, Gustavo C. Rocha, João Pessoa Araujo Junior, Valber A. Pedrosa Title The Notch ligand DLL4 specifically marks human hematoendothelial progenitors and regulates their hematopoietic fate Journal Leukemia. 2015. 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Authors Liron Zehavi1, Hagit Schayek, Jasmine Jacob-Hirsch, Yechezkel Sidi, Raya Leibowitz-Amit and Dror Avni Title Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung Journal Development. 2015. Authors Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat Title Phosphorylation-dependent degradation of MEF2C contributes to regulate G2/M transition Journal Cell Cycle. 2015. Authors Sara Badodi, Fiorenza Baruffaldi, Massimo Ganassi, Renata Battini & Susanna Molinari Title Highly specific role of the insulin receptor in breast cancer progression Journal Endocrine-Related Cancer. 2015. Authors Ran Rostoker, Sagi Abelson, Keren Bitton-Worms, Inna Genkin, Sarit Ben-Shmuel, Maria Dakwar, Zila Shen Orr, Avishay Caspi, Maty Tzukerman and Derek LeRoith Title N6-methyladenosine marks primary microRNAs for processing Journal Nature. 2015. Authors Claudio R. Alarcon, Hyeseung Lee, Hani Goodarzi, Nils Halberg & Sohail F. Tavazoie Title eIF6 over-expression increases the motility and invasiveness of cancer cells by modulating the expression of a critical subset of membrane-bound proteins Journal BMC Cancer. 2015. Authors Michela Pinzaglia, Claudia Montaldo, Dorina Polinari, Mattei Simone, Anna La Teana, Marco Tripodi, Carmine Mancone, Paola Londei, Dario Benelli Title Examining the Feasibility of Clinical Grade CD271+ Enrichment of Mesenchymal Stromal Cells for Bone Regeneration Journal PLoS ONE. 2015. Authors Richard J. Cuthbert, Peter V. Giannoudis, Xiao N. Wang, Lindsay Nicholson, David Pawson, Anatole Lubenko, Hiang B. Tan, Anne Dickinson, Dennis McGonagle, Elena Jones Title Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved Journal Journal of Biotechnology. 2015. Authors Andrea Bedford, Tao Chen, Evanna Huynh, Cuilan Zhu, Samantha Medeiros, Doug Wey, Cornelis de Lange, Julang Li Title Results, Questions, Perspectives of a Study on Human Polyomavirus BK and Molecular Actors in Prostate Cancer Development Journal Cancer Genomics Proteomics. 2015. Authors Mischitelli M, Bellizzi A, Anzivino E, Rodio DM, Sciarra A, Gentile V, Pietropaolo V Title Isolation and expression of a cadmium-resistant gene (SaFer) from Sedum alfredii Journal Journal of Zhejiang A and F University. 2015. Authors ZHAO Ting, HAN Xiaojiao, LIU Mingying, QIAO Guirong, JIANG Jing, JIANG Yancheng,ZHUO Renying Title Epigenetic biomarkers in the blood of patients with urological malignancies Journal Expert Review of Molecular Diagnostics. 2015. Authors Jörg Ellinger, Stefan C Müller, and Dimo Dietrich Title In vitro cytotoxicity analysis of doxorubicin-loaded/superparamagnetic iron oxide colloidal nanoassemblies on MCF7 and NIH3T3 cell lines Journal International Journal of Nanomedicine. 2015. Authors Katerina Tomankova, Katerina Polakova, Klara Pizova, Svatopluk Binder, Marketa Havrdova, Mary Kolarova, Eva Kriegova, Jana Zapletalova, Lukas Malina, Jana Horakova, Jakub Malohlava, Argiris Kolokithas-Ntoukas, Aristides Bakandritsos, Hana Kolarova, Radek Zboril Title Galnt1 Is Required for Normal Heart Valve Development and Cardiac Function Journal PLoS ONE. 2015. Authors E Tian, Sharon R. Stevens, Yu Guan, Danielle A. Springer, Stasia A. Anderson, Matthew F. Starost, Vyomesh Patel, Kelly G. Ten Hagen, Lawrence A. Tabak Title Intramuscular sex steroid hormones are associated with skeletal muscle strength and power in women with different hormonal status Journal Aging Cell. 2015. Authors Eija Pöllänen, Reeta Kangas, Mia Horttanainen, Paula Niskala, Jaakko Kaprio, Gillian Butler-Browne, Vincent Mouly, Sarianna Sipilä and Vuokko Kovanen Title Changes in circulating microRNA-126 during treatment with chemotherapy and bevacizumab predicts treatment response in patients with metastatic colorectal cancer. Journal British Journal of Cancer.. 2015. Authors T F Hansen, A L Carlsen, N H H Heegaard, F B Sørensen and A Jakobsen. Title Progesterone receptor expression in granulosa cells is suppressed by microRNA-378-3p. Journal Molecular and Cellular Endocrinology. 2015. Authors Derek Toms, Shengyu Xu, Bo Pan1, De Wu, Julang Li. Title Aged iPSCs display an uncommon mitochondrial appearance and fail to undergo in vitro neurogenesis Journal Aging. 2014. Authors Andrea Masotti, Antonella Celluzzi, Stefania Petrini, Enrico Bertini, Ginevra Zanni, and Claudia Compagnucci. Title Distinct microRNA expression in endometrial lymphocytes, endometrium, and trophoblast during spontaneous porcine fetal loss Journal Journal of Reproductive Immunology. 2014. Authors Mallikarjun Bidarimath, Andrew K. Edwards, Jocelyn M. Wessels, Kasra Khalaj, Rami T. Kridli, Chandrakant Tayade Title Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources Journal Archives of Microbiology. 2014. Authors Anna Casabianca, Chiara Orlandi, Federica Barbieri, Luigia Sabatini, Andrea Di Cesare, Davide Sisti, Sonia Pasquaroli, Mauro Magnani, Barbara Citterio Title Use of the Zebrafish Larvae as a Model to Study Cigarette Smoke Condensate Toxicity Journal PLoS ONE. 2014. Authors Lee D. Ellis, Evelyn C. Soo, John C. Achenbach, Michael G. Morash, Kelly H. Soanes Title Cell-selective modulation of the Drosophila neuromuscular system by a neuropeptide Journal Journal of Neurophysiology. 2014. Authors Kiel G Ormerod, Jacob L Krans, A. Joffre Mercier Title Expression of a retinoic acid receptor (RAR)-like protein in the embryonic and adult nervous system of a protostome species Journal Journal of Experimental Zoology. 2014. Authors Christopher J. Carter, Christopher Rand, Imtiaz Mohammad, Amanda Lepp, Nicholas Vesprini, Olivia Wiebe, Robert Carlone andGaynor E. Spencer Title Mitochondrial complex I and III gene mRNA levels in schizophrenia, and their relationship with clinical features Journal Journal of Molecular Psychiatry. 2014. Authors Süleyman Akarsu, Deniz Torun, Abdullah Bolu, Murat Erdem, Salih Kozan, Mehmet Ak, Hatice Akar and Özcan Uzun Title Molecular Characterization of Testicular Adrenal Rest Tumors in Congenital Adrenal Hyperplasia; Lesions with both Adrenocortical and Leydig Cell Features Journal The Journal of Clinical Endocrinology and Metabolism. 2014. Authors Evelien E.J.W. Smeets, Paul N. Span, Antonius E. van Herwaarden, Ron A. Wevers, Ad R.M.M. Hermus, Fred C.G.J. Sweep, and Hedi L. Claahsen-van der Grinten Title Role of Biofilm in Protection of the Replicative Form of Legionella pneumophila. Journal Current Microbiology. 2014. Authors Andreozzi E, Di Cesare A, Sabatini L, Chessa E, Sisti D, Rocchi M, Citterio B. Title Reversible Lineage‐specific Priming of Human Embryonic Stem Cells Can Be Exploited to Optimize the Yield of Differentiated Cells Journal Stem Cells. 2014. Authors Jung Bok Lee, Monica Graham, Tony J. Collins, Jong-Hee Lee, Seok-Ho Hong, Amie McNicol, Zoya Shapovalova and Mickie Bhatia Title A Novel Technology for Multiplex Gene Expression Analysis Directly from Whole Blood Samples Stabilized at Ambient Temperature Using an RNA-Stabilizing Buffer Journal The Journal of Molecular Diagnostics. 2014. Authors Kim CH, Abedi M, Liu Y, Panuganti S, Flores F, Shah KR, Catterall H, Morampudi KS, Terbrueggen R Title The Circulating Cell-free microRNA Profile in Systemic Sclerosis Is Distinct from Both Healthy Controls and Systemic Lupus Erythematosus. Journal The Journal of Rheumatology. 2014. Authors Samantha O. Steen, Line V. Iversen, Anting Liu Carlsen, Mark Burton, Christoffer T. Nielsen, Søren Jacobsen and Niels H.H. Heegaard. Title Innate immune response of human pluripotent stem cell-derived airway epithelium. Journal Innate Immunity. 2014. Authors McIntyre BA, Kushwah R, Mechael R, Shapovalova Z, Alev C, Bhatia M. Title Chemokine-Mediated Robust Augmentation of Liver Engraftment: A Novel Approach Journal Stem Cells Translational Medicine. 2014. Authors Joshi M, Oltean M, Patil PB, Hallberg D, Kleman M, Holgersson J, Olausson M, Sumitran-Holgersson S Title Biological and molecular characterisation of Bidens mosaic virus supports its assignment as a member of a distinct species in the genus Potyvirus. Journal Archives of Virology. 2014. Authors Marcio Martinello Sanches, Bruno R. De Marchi, David Marques Spadotti, Denise Nakada Nozaki, Marcelo A. Pavan, Renate Krause-Sakate. Title C-MYC and C-FOS expression changes and cellular aspects of the photodynamic reaction with photosensitizers TMPyP and ClAlPcS2. Journal Journal of Photochemistry and Photobiology B: Biology. 2014. Authors Klara Pizova, Robert Bajgar, Regina Fillerova, Eva Kriegova, Vera Cenklova, Katerina Langova, Petr Konecny, Hana Kolarova. Title Identification and sequence analysis of five allexiviruses species infecting garlic crops in Brazil. Journal Tropical Plant Pathology. 2014. Authors Milena L. Oliveira; Bruno R. De Marchi; Tatiana Mituti; Marcelo A. Pavan; Renate Krause-Sakate. Title Associations between gastrointestinal toxicity, micro RNA and cytokine production in patients undergoing myeloablative allogeneic stem cell transplantation. Journal International Immunopharmacology. 2014. Authors Peter L. Pontoppidan, Karina Jordan, Anting Liu Carlsen, Hilde Hylland Uhlving, Katrine Kielsen, Mette Christensen, Marianne Ifversen, Claus Henrik Nielsen, Per Sangild, Niels Henrik Helweg Heegaard, Carsten Heilmann, Henrik Sengeløv, Klaus Müller. Title The oral lipid sensor GPR120 is not indispensable for the orosensory detection of dietary lipids in the mouse. Journal Journal of Lipid Research 2014. Authors Deborah ANCEL, Arnaud BERNARD, Selvakumar SUBRAMANIAM, Akira HIRASAWA, Gozoh TSUJIMOTO, Toshihiro HASHIMOTO, Patricia PASSILLY-DEGRACE, Naim-Akhtar KHAN and Philippe BESNARD. Title Circulating miR-21, miR-146a and Fas ligand respond to postmenopausal estrogen-based hormone replacement therapy – A study with monozygotic twin pairs. Journal Mechanisms of Ageing and Development. 2014. Authors Reeta Kangas, Eija Pöllänen, Maria Rita Rippo, Catia Lanzarini, Francesco Prattichizzo, Paula Niskala, Juulia Jylhävä, Sarianna Sipilä, Jaakko Kapri, Antonio Domenico Procopio, Miriam Capri, Claudio Franceschi, Fabiola Olivieri, Vuokko Kovanen. Title Sialylation of N-Linked Glycans Influences the Immunomodulatory Effects of IgM on T Cells. Journal The Journal of Immunology. 2014. Authors Manuela Colucci, Henning Stockmann, Alessia Butera, Andrea Masotti, Antonella Baldassarre, Ezio Giorda, Stefania Petrini, Pauline M. Rudd, Roberto Sitia, Francesco Emma and Marina Vivarelli. Title Rapid regulation of microRNA following induction of long-term potentiation in vivo. Journal Frontiers in Molecular Neuroscience. 2014. Authors Greig Joilin, Diane Guévremont, Brigid Ryan, Charles Claudianos, Alexandre S. Cristino, Wickliffe C. Abraham and Joanna M. Williams. Title Diabetes Enhances the Proliferation of Adult Pancreatic Multipotent Progenitor Cells and Biases Their Differentiation to more β-Cell Production. Journal Diabetes. 2014. Authors Rozita Razavi, Hamed S Najafabadi, Sarah Abdullah, Simon Smukler, Margot Arntfield and Derek van der Kooy. Title Oscillating glucose and constant high glucose induce endoglin expression in endothelial cells: the role of oxidative stress. Journal Acta Diabetologica. 2014. Authors Lucia La Sala, Gemma Pujadas, Valeria De Nigris, Silvia Canivell, Anna Novials, Stefano Genovese, Antonio Ceriello. Title Cloning of nitric oxide associated 1 (NOA1) transcript from oil palm (Elaeis guineensis) and its expression during Ganoderma infection. Journal Journal of Plant Physiology. 2014. Authors Yee-Min Kwan, Sariah Meon, Chai-Ling Ho, Mui-Yun Wong. Title microRNA-320/RUNX2 axis regulates adipocytic differentiation of human mesenchymal (skeletal) stem cells. Journal Nature. 2014. Authors D Hamam1,3, D Ali, R Vishnubalaji, R Hamam, M Al-Nbaheen, L Chen, M Kassem, A Aldahmash and NM Alajez. Title MicroRNA-378 Regulates Adiponectin Expression in Adipose Tissue: A New Plausible Mechanism. Journal PLOS ONE. 2014. Authors Masayoshi Ishida, Michio Shimabukuro, Shusuke Yagi, Sachiko Nishimoto, Chisayo Kozuka, Daiju Fukuda, Takeshi Soeki, Hiroaki Masuzaki, Masato Tsutsui, Masataka Sata. Title Citrullus lanatus is a New Natural Host of Groundnut ringspot virus in Brazil. Journal Journal of Phytopathology. 2014. Authors Evelynne Urzêdo Leão, David Marques de Almeida Spadotti, Kelly Cristina Gonçalves Rocha, Késsia de Fátima da Cunha Pantoja, Jorge Alberto Marques Rezende, Marcelo Agenor Pavan and Renate Krause-Sakate. Title Macrophage deficiency of Akt2 reduces atherosclerosis in LDLR null mice. Journal The Journal of Lipid Research. 2014. Authors Vladimir R. Babaev, Katie E. Hebron1, Carrie B. Wiese, Cynthia L. Toth, Lei Ding, Youmin Zhang, James M. May, Sergio Fazio, Kasey C. Vickers and MacRae F. Linton. Title MicroRNA-223 coordinates cholesterol homeostasis. Journal Proceedings of the National Academy of Sciences of the United States of America (PNAS). 2014. Authors Kasey C. Vickers, Stuart R. Landstreet, Michael G. Levin, Bassem M. Shoucri, Cynthia L. Toth, Robert C. Taylor, Brian T. Palmisano, Fatiha Tabet, Huanhuan L. Cui, Kerry-Anne Rye, Praveen Sethupathy, and Alan T. Remaley. Title Tumor Microenvironmental Changes Induced by the Sulfamate Carbonic Anhydrase IX Inhibitor S4 in a Laryngeal Tumor Model. Journal PLOS One. 2014. Authors Tineke W. H. Meijer, Johan Bussink, Miriam Zatovicova, Paul N. Span, Jasper Lok, Claudiu T. Supuran, Johannes H. A. M. Kaanders. Title The Crosstalk between IL-22 Signaling and miR-197 in Human Keratinocytes. Journal PLOS One. 2014. Authors Galya Lerman, Moran Sharon, Raya Leibowitz-Amit, Yechezkel Sidi, Dror Avni. Title The expression of Hox genes, zen (GB 51301) ANT and UBX during the development of honey bee (Apis mellifera). Journal American Journal of BioScience. 2014. Authors Mariapia Viola-Magni, Samuela Cataldi. Title Glucose Metabolism in NSCLC Is Histology-Specific and Diverges the Prognostic Potential of 18FDG-PET for Adenocarcinoma and Squamous Cell Carcinoma.. Journal Journal of Thoracic Oncology. 2014. Authors Schuurbiers, Olga C. J.; Meijer, Tineke W. H.; Kaanders, Johannes H. A. M.; Looijen-Salamon, Monika G.; de Geus-Oei, Lioe-Fee; van der Drift, Miep A.; van der Heijden, Erik H. F. M.; Oyen, Wim J.; Visser, Eric P.; Span, Paul N.; Bussink, Johan. Title miR-182 attenuates atrophy-related gene expression by targeting FoxO3 in skeletal muscle. Journal American Journal of Physiology - Cell Physiology. 2014. Authors Matthew B. Hudson , Jill A. Rahnert , Bin Zheng , Myra E. Woodworth-Hobbs , Harold A. Franch , S. Russ Price. Title Expression Profile of Maize MicroRNAs Corresponding to Their Target Genes Under Drought Stress. Journal Biochemical Genetics 2014. Authors Wang YG, An M, Zhou SF, She YH, Li WC, Fu FL. Title Metastasis-suppressor transcript destabilization through TARBP2 binding of mRNA hairpins Journal Nature. 2014. Authors Hani Goodarzi, Steven Zhang, Colin G. Buss, Lisa Fish, Saeed Tavazoie & Sohail F. Tavazoie. Title The Novel Role of HtrA1 in Gingivitis, Chronic and Aggressive Periodontitis Journal PLOS ONE. 2014. Authors Teresa Lorenzi, Elena Annabel Niţulescu, Antonio Zizzi, Maria Lorenzi, Francesca Paolinelli, Simone Domenico Aspriello, Monica Baniţă, Ştefania Crăiţoiu, Gaia Goteri, Giorgio Barbatelli, Tommaso Lombardi, Roberto Di Felice, Daniela Marzioni mail, Corrado Rubini, Mario Castellucci. Title miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export. Journal American Journal of Physiology. Cell Physiology.. 2014. Authors Hudson MB, Woodworth-Hobbs ME, Zheng B, Rahnert JA, Blount MA, Gooch JL, Searles CD, Price SR. Title Hypoxic Regulation of the PERK/ATF4/LAMP3-arm of the Unfolded Protein Response in Head and Neck Squamous Cell Carcinoma. Journal Head & Neck. 2014. Authors Nagelkerke A, Sweep FC, Stegeman H, Grénman R, Kaanders JH, Bussink J, Span PN. Title First report of Groundnut ringspot virus in cucumber fruits in Brazil Journal New Disease Reports. 2014. Authors Spadotti D.M.A., Leäo E.U., Rocha K.C.G., Pavan M.A.and R. Krause-Sakate Title Activation of the developmental pathway Neurogenin-3/microRNA-7a regulates cholangiocyte proliferation in response to injury. Journal Hepatology. 2014. Authors Marco Marzioni, Laura Agostinelli, Cinzia Candelaresi, Stefania Saccomanno, Samuele De Minicis, Luca Maroni, Eleonora Mingarelli, Chiara Rychlicki, Luciano Trozzi, Jesus M. Banales, Antonio Benedetti and Gianluca Svegliati Baroni. Title Cytotoxicity and Antioxidative Effects of Herbal and Fruit Extracts In Vitro. Journal Food Biophysics. 2014. Authors Katerina Tomankova, Hana Kolarova, Klara Pizova, Svatopluk Binder, Petr Konecny, Eva Kriegova, Lukas Malina, Jana Horakova, Jakub Malohlava, Kristina Kejlova, Dagmar Jirova. Title Effects of shear stress pattern and magnitude on mesenchymal transformation and invasion of aortic valve endothelial cells. Journal Biotechnology and Bioengineering. 2014. Authors Mahler GJ, Frendl CM, Cao Q, Butcher JT. Title Comparison Of Two Total RNA Extraction Protocols From Cho-K1 Cells For Rt-PCR: Cutoff Cost For Researchers. Journal IIUM Engineering Journal. 2014. Authors Mohamed V, Hashim Y, Amid A, and Mel M. Title Bacterial autolysins trim cell surface peptidoglycan to prevent detection by the Drosophila innate immune system. Journal Elife. 2014. Authors Atilano ML, Pereira PM, Vaz F, Catalão MJ, Reed P, Grilo IR, Sobral RG, Ligoxygakis P, Pinho MG, Filipe SR. Title Profiling of MicroRNAs under Wound Treatment in Aquilaria sinensis to Identify Possible MicroRNAs Involved in Agarwood Formation. Journal International Journal of Biological Sciences. 2014. Authors Gao ZH, Yang Y, Zhang Z, Zhao WT, Meng H, Jin Y, Huang JQ, Xu YH, Zhao LZ, Liu J, Wei JH. Title Engineered zinc-finger transcription factors activate OCT4 (POU5F1), SOX2, KLF4, c-MYC (MYC) and miR302/367. Journal Nucleic Acids Research. 2014. Authors Ji Q, Fischer AL, Brown CR, Eastlund ER, Dvash T, Zhong B, Gerber MA, Lyons I, Knight SW, Kreader CA. Title Molecular evidence for OCT4 induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors. Journal Stem Cells. 2014. Authors Mitchell R, Szabo E, Shapovalova Z, Aslostovar L, Makondo K, Bhatia M. Title Human placenta-derived neurospheres are susceptible to transformation after extensive in vitro expansion. Journal Stem Cell Research and Therapy. 2014. Authors Amendola D, Nardella M, Guglielmi L, Cerquetti L, Carico E, Alesi V, Porru M, Leonetti C, Bearzi C, Rizzi R, D Agnano I, Stigliano A, Novelli G, Bucci B. Title A potentially novel nicotinic receptor 2 in Aplysia neuroendocrine cells. Journal Journal of Neurophysiology. 2014. Authors White SH, Carter CJ, Magoski NS. Title microRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes. Journal Diabetes. 2014. Authors Kurtz CL, Peck BC, Fannin EE, Beysen C, Miao J, Landstreet SR, Ding S, Turaga V, Lund PK, Turner S, Biddinger SB, Vickers KC, Sethupathy P. Title Characterization and in-vitro differentiation potency of early-passage canine amnion- and umbilical cord-derived mesenchymal stem cells as related to gestational age. Journal Molecular Reproduction and Development.. 2014. Authors Filioli Uranio M, Dell'aquila M, Caira M, Guaricci A, Ventura M, Catacchio C, Martino N, Valentini L. Title Enzyme Treatment RT-PCR to Differentiate Infectious and Inactivated F+RNA Phages. Journal Applied and Environmental Microbiology. 2014. Authors Yang Y, Griffiths MW. Title MiRNome expression is deregulated in the peripheral lymphoid compartment of multiple myeloma. Journal British Journal of Haematology. 2014. Authors Campo S, Allegra A, D'Ascola A, Alonci A, Scuruchi M, Russo S, Avenoso A, Gerace D, Campo GM, Musolino C. Title De Novo Assembly and Annotation of Salvia splendens Transcriptome Using the Illumina Platform. Journal PLOS One. 2014. Authors Ge X, Chen H, Wang H, Shi A, Liu K. Title Enhanced cold stress tolerance of transgenic Dendrocalamus latiflorusMunro (Ma bamboo) plants expressing a bacterial CodA gene Journal In Vitro Cellular & Developmental Biology - Plant. 2014. Authors Qiao G, Yang H, Zhang L, Han X, Liu M, Jiang J, Jiang Y, Zhuo R. Title Ovarian cell-like cells from skin stem cells restored estradiol production and estrus cycling in ovariectomized mice. Journal Stem Cells and Development. 2014. Authors Park BW, Pan B, Toms D, Huynh E, Byun JH, Lee YM, Shen W, Rho GJ, Li J. Title The effect of heme oxygenase on ganglioside redistribution within hepatocytes in experimental estrogen-induced cholestasis Journal Physiological Research. 2014. Authors Petr T, SmÍd V, Kučerová V, Váňová K, Leníček M, VÍtek L, SmÍd F, Muchová L. Title HDL-transferred microRNA-223 regulates ICAM-1 expression in endothelial cells. Journal Nature Communications. 2014. Authors: Tabet F, Vickers KC, Cuesta Torres LF, Wiese CB, Shoucri BM, Lambert G, Catherinet C, Prado-Lourenco L, Levin MG, Thacker S, Sethupathy P, Barter PJ, Remaley AT, Rye KA. Title Broad-Spectrum Therapeutic Suppression of Metastatic Melanoma through Nuclear Hormone Receptor Activation. Journal Cell. 2014. Authors Pencheva N, Buss CG, Posada J, Merghoub T, Tavazoie SF. Title Protective Effect of Melatonin on Infrarenal Aortic Occlusion: This Effect Is Related to Anti-inflammatory Effect and Antioxidant Effect. Journal Inflammation. 2014. Authors Ahsen A, Gonul Y, Genc A, Ulu MS, Yagmurca M, Kocogullari CU, Celik S, Yuksel S. Title Mode of Action of a Drosophila FMRFamide in Inducing Muscle Contraction. Journal The Journal of Experimental Biology. 2014. Authors Milakovic M, Ormerod KG, Klose MK, Mercier J. Title SNP rs8099917 in Gene IL28B Might Be Associated with Risk of Chronic Infection by HCV but Not with Response to Treatment. Journal BioMed Research International. 2014. Authors Conde S, Monteiro J, Santos B, Filgueiras N, Lins P, Freitas F, Graça E, Demachki S, Araújo M, Ishak R, Vallinoto A Title Conserved miRNAs and Their Response to Salt Stress in Wild Eggplant Solanum linnaeanum Root. Journal International Journal of Molecular Science. 2014. Authors Zhuang Y, Zhou XH, Liu J. Title Growth performance of early-weaned pigs is enhanced by feeding epidermal growth factor -expressing Lactococcus lactis fermentation product. Journal Journal of Biotechnology. 2014. Authors Bedford A, Huynh E, Fu M, Zhu C, Wey D, Lange C, Li J Title LAMP3 is involved in tamoxifen resistance in breast cancer cells through the modulation of autophagy. Journal Endocrine-Related Cancer. 2014. Authors Nagelkerke A, Sieuwerts AM, Bussink J, Sweep FC, Look MP, Foekens JA, Martens JW, Span PN. Title High Serum miR-19a Levels Are Associated with Inflammatory Breast Cancer and Are Predictive of Favorable Clinical Outcome in Patients with Metastatic HER2+ Inflammatory Breast Cancer. Journal PLOS One. 2013. Authors Anfossi S, Giordano A, Gao H, Cohen E, Tin S, Wu Q, Garza R, Debeb B, Alvarez R, Valero V, Hortobagyi G, Calin G, Ueno N, Woodward W, Reuben J. Title Deciphering Small Noncoding RNAs during the Transition from Dormant Embryo to Germinated Embryo in Larches (Larix leptolepis) Journal PLOS One. 2013. Authors Sang J, Han X, Liu M, Qiao G, Jiang J, Zhu R Title Molecules Altering the Intracellular Thiol Content Modulate NF-kB and STAT-1/IRF-1 Signalling Pathways and IL-12 p40 and IL-27 p28 Production in Murine Macrophages. Journal PLoS One. 2013. Authors Fraternale A, Crinelli R, Casabianca A, Paoletti MF, Orlandi C, Carloni E, Smietana M, Palamara AT, Magnani M. Title MicroRNA-22 Is a Master Regulator of Bone Morphogenetic Protein-7/6 Homeostasis in the Kidney. Journal The Journal of Biological Chemistry. 2013. Authors Long J, Badal SS, Wang Y, Chang BH, Rodriguez A, Danesh FR. Title ABCA1 C69T gene polymorphism and risk of type 2 diabetes mellitus in a Saudi population. Journal Journal of Bioscience. 2013. Authors Alharbi K, Khan I, Al-Daghri N, Munshi A, Sharma V, Mohammed A, Wani K, Al-Sheikh Y, Al-Nbaheen M, Ahmed Ansari M, Syed R. Title Derivation of Neural Stem Cells from Human Adult Peripheral CD34+ Cells for an Autologous Model of Neuroinflammation Journal PLOS One. 2013. Authors Wang T, Choi E, Monaco M, Campanac E, Medynets M, Do T, Rao P, Johnson K, Elkahloun A, Geldern G, Johnson T, Subramaniam S, Hoffman D, Major E, Nath A Title Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano. Journal The IMSE Journal. 2013. Authors Meyer JL, Huber JA. Title Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Journal Nucleic Acids Research. 2013. Authors Espah Borujeni A, Channarasappa AS, Salis HM. Title Novel Di-tertiary-Butyl Phenylhydrazones as Dual Cyclooxygenase- 2/ 5-Lipoxygenase inhibitors: Synthesis, COX/LOX Inhibition, Molecular modeling, and Insights into their Cytotoxicities Journal Bioorganic & Medicinal Chemistry Letters. 2013. Authors Ghatak S, Vyas A, Misra S, O’Brien P, Zambre A, Fresco V, Markwald R, Swamy K, Afrasiabi Z, Choudhury A, Khetmalas M, Padhye S Title Isolation and Characterization of Porcine Epidemic Diarrhea Viruses Associated with the 2013 Disease Outbreak in US Swine. Journal Journal of Clinical Microbiology. 2013. Authors Chen Q, Li G, Stasko J, Thomas JT, Stensland WR, Pillatzki AE, Gauger PC, Schwartz KJ, Madson D, Yoon KJ, Stevenson GW, Burrough ER, Harmon KM, Main RG, Zhang J. Title Human fetal and adult bone marrow derived mesenchymal stem cells use different signalling pathways for the initiation of chondrogenesis. Journal Stem Cells and Development. 2013. Authors Brady K, Dickinson SC, Guillot PV, Polak JM, Blom AW, Kafienah W, Hollander AP. Title Protective effects of inhaled carbon monoxide in endotoxin-induced cholestasis is dependent on its kinetic. Journal Biochimie. 2013. Authors Vanova K, Suk J, Petr T, Cerny D, Slanar O, Vremanc H, Wong R, Zima T, Vitek L, Muchova L. Title mRNAs and miRNAs profiling of mesenchymal stem cells derived from amniotic fluid and skin: the double face of the coin. Journal Cell and Tissue Research. 2013. Authors Lazzarini R, Olivieri F, Ferretti C, Mattioli-Belmonte M, Di Primio R, Orciani M. Title Circulating microRNAs and Kallikreins before and after Radical Prostatectomy: Are They Really Prostate Cancer Markers? Journal BioMed Research International. 2013. Authors Egidi M, Cochetti G, Serva M, Guelfi G, Zampini D, Mechelli L, and Mearini E. Title First Report of Garlic virus X in Garlic plants in Brazil. Journal Plant Disease. 2013. Authors Oliveira M, Hoffmann M, Mituti T, Pavan M and Krause-Sakate R. Title Induced periosteum a complex cellular scaffold for the treatment of large bone defects. Journal Bone. 2013. Authors Cuthbert RJ, Churchman SM, Tan HB, McGonagle D, Jones E, Giannoudis PV. Title In vitro morphine metabolism by rat microglia. Journal Neuropharmacology. 2013. Authors Togna AR, Antonilli L, Dovizio M, Salemme A, De Carolis L, Togna GI, Patrignani P, Nencini P. Title Expression, Tissue Distribution and Function of miR-21 in Esophageal Squamous Cell Carcinoma. Journal PLOS One. 2013. Authors Nouraee N, Van Roosbroeck K, Vasei M, Semnani S, Samaei NM, Naghshvar F, Omidi AA, Calin GA, Mowla SJ. Title Hemochromatosis Enhances Tumor Progression via Upregulation of Intracellular Iron in Head and Neck Cancer Journal PLOS One. 2013. Authors Lenarduzzi M, Hui AB, Yue S, Ito E, Shi W, Williams J, Bruce J, Sakemura-Nakatsugawa N, Xu W, Schimmer A, Liu FF. Title Beta Cell 5'-Shifted isomiRs Are Candidate Regulatory Hubs in Type 2 Diabetes. Journal PLOS One. 2013. Authors Baran-Gale J, Fannin EE, Kurtz CL, Sethupathy P. Title Expression analysis of glycosyltransferase BcUGT1 from Bupleurum chinese DC. and its expression in E. coli and the target protein purification Journal Acta Pharmaceutica Sinica. 2013. Authors Tao Y, Xu J, Wei J, Sun J, Xu Y, Yang X, Zhang Y, Liu J, Sui C.* Title Effect of Oviduct Epithelial Cells Co-culture on the Developmental Arrest of Early Embryos in Mice Journal Acta Pharmaceutica Sinica. 2013. Authors Zhaung L, Wu J, Hu D, Cai L, Zhang Y, Li Z, Fang N. Title Ciliated cultures from PCD patients do not produce NO or iNOS during early infection. Journal Chest. 2013. Authors Smith CM, Fadaee-Shohada MJ, Sawhney R, Baker N, Willliams G, Hirst RA, Andrew PW, O'Callaghan C. Title Voltage-gated Ca2+ influx and mitochondrial Ca2+ initiate secretion from Aplysia neuroendocrine cells. Journal Neuroscience. 2013. Authors Hickey CM, Groten CJ, Sham L, Carter CJ, Magoski NS. Title Transcriptome Characteristics and Six Alternative Expressed Genes Positively Correlated with the Phase Transition of Annual Cambial Activities in Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook) Journal PLOS One. 2013. Authors Wang Z, Chen J, Liu W, Luo Z, Wang P, Zhang Y, Zheng R, Shi J. Title Differential Expression of MicroRNAs in Response to Drought Stress in Maize. Journal Journal of Integrative Agriculture. 2013. Authors Li J, Fu F, An M, Zhou S, She Y, Li W. Title Pyrimidine homeostasis is accomplished by directed overflow metabolism. Journal Nature. 2013. Authors Reaves ML, Young BD, Hosios AM, Xu YF, Rabinowitz JD. Title Thrombocytopenia induced by the histone deacetylase inhibitor abexinostat involves p53-dependent and -independent mechanisms. Journal Cell Death and Disease. 2013. Authors Ali A, Bluteau O, Messaoudi K, Palazzo A, Boukour S, Lordier L, Lecluse Y, Rameau P, Kraus-Berthier L, Jacquet-Bescond A, Lelièvre H, Depil S, Dessen P, Solary E, Raslova H, Vainchenker W, Plo I, Debili N. Title Prognostic value of miR-155 in individuals with monoclonal B-cell lymphocytosis and patients with B-chronic lymphocytic leukemia. Journal Blood. 2013. Authors Ferrajoli A, Shanafelt TD, Ivan C, Shimizu M, Rabe KG, Nouraee N, Ikuo M, Ghosh AK, Lerner S, Rassenti LZ, Xiao L, Hu J, Reuben JM, Calin S, You MJ, Manning JT, Wierda WG, Estrov Z, O'Brien S, Kipps TJ, Keating MJ, Kay NE, Calin GA. Title Suitability of Human Tenon's Fibroblasts as Feeder Cells for Culturing Human Limbal Epithelial Stem Cells.. Journal Stem Cell Reviews. 2013. Authors Scafetta G, Tricoli E, Siciliano C, Napoletano C, Puca R, Vingolo EM, Cavallaro G, Polistena A, Frati G, De Falco E. Title MicroRNA-196b Regulates the Homeobox B7-Vascular Endothelial Growth Factor Axis in Cervical Cancer. Journal PLOS One. 2013. Authors How C, Hui A, Alajez N, Shi W, Boutros P, Clarke B, Yan R, Pintilie M, Fyles A, Hedley D, Hill R, Milosevic M, Liu F Title Bacterial Adaptation through Loss of Function. Journal PLOS Genetics. 2013. Authors Hottes A, Freddolino P, Khare A, Donnell Z, Liu J, Tavazoie S Title Identification of circulating microRNAs in HNF1A-MODY carriers. Journal Diabetologia. 2013. Authors Bonner C, Nyhan KC, Bacon S, Kyithar MP, Schmid J, Concannon CG, Bray IM, Stallings RL, Prehn JH, Byrne MM. Title Polymorphisms in metalloproteinase-9 are associated with the risk for asthma in Mexican pediatric patients. Journal Human Immunology. 2013. Authors Jiménez-Morales S, Martínez-Aguilar N, Gamboa-Becerra R, Jiménez-Ruíz JL, López-Ley D, Lou H, Saldaña-Alvarez Y, Dean M, Orozco L. Title Cholesterol affects gene expression of the Jun family in colon carcinoma cells using different signaling pathways. Journal Molecular and Cellular Endocrinology. 2013. Authors Scheinman EJ, Rostoker R, Leroith D. Title miR-21 represses Pdcd4 during cardiac valvulogenesis. Journal Development. 2013. Authors Kolpa HJ, Peal DS, Lynch SN, Giokas AC, Ghatak S, Misra S, Norris RA, Macrae CA, Markwald RR, Ellinor P, Bischoff J, Milan DJ. Title Circulating microRNA expression profiles associated with systemic lupus erythematosus. Journal Arthritis and Rheumatism. 2013. Authors Carlsen AL, Schetter AJ, Nielsen CT, Lood C, Knudsen S, Voss A, Harris CC, Hellmark T, Segelmark M, Jacobsen S, Bengtsson AA, Heegaard NH. Title Prognostic significance of serum microRNA-221 expression in human epithelial ovarian cancer. Journal The Journal of International Medical Research. 2013. Authors Hong F, Li Y, Xu Y, ZhuL. Title Gli3-mediated hedgehog inhibition in human pluripotent stem cells initiates and augments developmental programming of adult hematopoiesis. Journal Blood. 2013. Authors McIntyre BA, Ramos-Mejia V, Rampalli S, Mechael R, Lee JH, Alev C, Sheng G, Bhatia M. Title Rat mir-155 generated from the lncRNA Bic is 'hidden' in the alternate genomic assembly and reveals the existence of novel mammalian miRNAs and clusters. Journal RNA: A Publication of the RNA Society. 2013. Authors Uva P, Da Sacco L, Del Cornò M, Baldassarre A, Sestili P, Orsini M, Palma A, Gessani S, Masotti A. Title MicroRNA-193b Enhances Tumor Progression via Down Regulation of Neurofibromin 1. Journal PLOS One. 2013. Authors Lenarduzzi M, Hui AB, Alajez NM, Shi W, Williams J, Yue S, O'Sullivan B, Liu FF. Title Evaluation of carrier-mediated siRNA delivery: Lessons for the design of a stem-loop qPCR-based approach for quantification of intracellular full-length siRNA. Journal Journal of Controlled Release. 2013. Authors Colombo S, Nielsen HM, Foged C. Title Solithromycin Inhibition of Protein Synthesis and Ribosome Biogenesis in Staphylococus aureus, Streptococcus pneumoniae, and Haemophilus influenzae. Journal Antimicrobial Agents and Chemotherpy. 2013. Authors Rodgers W, Frazier AD, Champney WS. Title A K(ATP) channel gene effect on sleep duration: from genome-wide association studies to function in Drosophila. Journal Molecular Psychiatry. 2013. Authors Allebrandt KV, Amin N, Müller-Myhsok B, Esko T, Teder-Laving M, Azevedo RV, Hayward C, van Mill J, Vogelzangs N, Green EW, Melville SA, Lichtner P, Wichmann HE, Oostra BA, Janssens AC, Campbell H, Wilson JF, Hicks AA, Pramstaller PP, Dogas Z, Rudan I, Merrow M, Penninx B, Kyriacou CP, Metspalu A, van Duijn CM, Meitinger T, Roenneberg T.. Title Hypoxia stimulates migration of breast cancer cells via the PERK/ATF4/LAMP3-arm of the unfolded protein response. Journal Breast Cancer Research: BCR. 2012. Authors Nagelkerke A, Bussink J, Mujcic H, Wouters BG, Lehmann S, Sweep FC, Span PN. Title Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth. Journal The Plant Journal: for cell and molecular biology. 2012. Authors Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH. Title Impact of the redox-cycling herbicide diquat on transcript expression and antioxidant enzymatic activities of the freshwater snail Lymnaea stagnalis. Journal Aquatic Toxicology. 2012. Authors Bouétard A, Besnard AL, Vassaux D, Lagadic L, Coutellec MA. Title Regulation of TRIB3 mRNA and Protein in Breast Cancer. Journal PLOS One. 2012. Authors Wennemers M, Bussink J, van den Beucken T, Sweep FC, Span PN. Title Deleted in Azoospermia-Like (DAZL) enhances in vitro-derived porcine germ cell formation and meiosis. Journal Stem Cells and Development. 2012. Authors Park B, Shen W, Linher-Melville K, and Li J. Title Identification of wild soybean miRNAs and their target genes responsive to aluminum stress. Journal BMC Plant Biology. 2012. Authors Qiao-Ying Z, Cun-Yi Y, Qi-Bin M, Xiu-Ping L, Wen-Wen D, Hai N. Title Age-related differences in the expression of circulating microRNAs: miR-21 as a new circulating marker of inflammaging. Journal Mechanisms of Aging and Development. 2012. Authors Olivieri F, Spazzafumo L, Santini G, Lazzarini R, Albertini MC, Rippo MR, Galeazzi R, Abbatecola AM, Marcheselli F, Monti D, Ostan R, Cevenini E, Antonicelli R, Franceschi C, Procopio AD. Title The TspanC8 subgroup of tetraspanins interact with A disintegrin and metalloprotease 10 (ADAM10) and regulate its maturation and cell surface expression. Journal The Journal of Biological Chemistry. 2012. Authors Haining EJ, Yang J, Bailey RL, Khan K, Collier R, Tsai S, Watson SP, Frampton J, Garcia P, Tomlinson MG. Title Dynamic expression of small RNA populations in larch (Larix leptolepis). Journal Planta. 2012. Authors Zhang J, Wu T, Li L, Han S, Li X, Zhang S, Qi L. Title Hypomorphic Sialidase expression decreases serum cholesterol by downregulationof VLDL production in Mice. Journal Journal of Lipid Research. 2012. Authors Yang A, Gyulay G, Mitchell M, White E, Trigatti BL, Igdoura SA. Title Proliferation and recapitulation of developmental patterning associated with regulative regeneration of the spinal cord neural tube. Journal Developmental Biology. 2012. Authors Halasi G, Søviknes AM, Sigurjonsson O, Glover JC. Title Method for Microrna Isolation from Clinical Serum Samples. Journal Analytical Biochemistry. 2012. Authors Li Y, Kowdley KV. Title Designing and using RNA scaffolds to assemble proteins in vivo. Journal Nature Protocols. 2012. Authors Delebecque CJ, Silver PA, Lindner AB. Title Deep Sequencing and Microarray Hybridization Identify Conserved and Species-Specific MicroRNAs during Somatic Embryogenesis in Hybrid Yellow Poplar. Journal PLOS One. 2012. Authors Li T, Chen J, Qiu S, Zhang Y, Wang P, Yang L, Lu Y, Shi J. Title Potentially Novel Candidate Biomarkers for Head and Neck Squamous Cell Carcinoma Identified Using an Integrated Cell Line-based Discovery Strategy. Journal Molecular and Cellular Proteonomics. 2012. Authors Sepiashvili L, Hui A, Ignatchenko V, Shi W, Su S, Xu W, Huang SH, O'Sullivan B, Waldron J, Irish JC, Perez-Ordonez B, Liu FF, Kislinger T. Title Human embryonic stem cell-derived hematopoietic cells maintain core epigenetic machinery of the PcG/TrxG complexes distinctly from functional adult HSCs. Journal Clinical Cancer Research. 2012. Authors Schnerch A, Lee JB, Graham M, Guezguez B, Bhatia M. Title Pyrosequencing-based transcriptomic resources in the pond snail Lymnaea stagnalis, with a focus on genes involved in molecular response to diquat-induced stress. Journal Ecotoxicology. 2012. Authors Bouétard A, Noirot C, Besnard AL, Bouchez O, Choisne D, Robe E, Klopp C, Lagadic L, Coutellec MA. Title The role of heterodimerization between VEGFR-1 and VEGFR-2 in the regulation of endothelial cell homeostasis. Journal Nature Communications. 2012. Authors Cudmore MJ, Hewett PW, Ahmad S, Wang KQ, Cai M, Al-Ani B, Fujisawa T, Ma B, Sissaoui S, Ramma W, Miller MR, Newby DE, Gu Y, Barleon B, Weich H, Ahmed A. Title Temporal Profiling of Gene Networks Associated with the Late Phase of Long-Term Potentiation In Vivo. Journal PLOS ONE. 2012. Authors Ryan MM, Ryan B, Kyrke-Smith M, Logan B, Tate WP, Abraham W, Williams J. Title Combinatorial Action of miRNAs Regulates Transcriptional and Post-Transcriptional Gene Silencing following in vivo PNS Injury. Journal PLOS ONE. 2012. Authors Adilakshmi T, Sudol I, Tapinos. Title IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut. Journal Mucosal Immunology. 2012. Authors Manocha M, Shajib M S, Rahman M M, Wang H, Rengasamy P, Bogunovic M, Jordana M, Mayer L and I KhanR W. Title Expression profile of miRNAs in Populus cathayana L. and Salix matsudana Koidz under salt stress. Journal Molecular Biology Reports. 2012. Authors Zhou J, Liu M, Jiang J, Qiao G, Lin S, Li H, Xie L, Zhuo R. Title MiR-146a as marker of senescence-associated pro-inflammatory status in cells involved in vascular remodelling. Journal Age (Dordrecht, Netherlands). 2012. Authors Olivieri F, Lazzarini R, Recchioni R, Marcheselli F, Rippo MR, Di Nuzzo S, Albertini MC, Graciotti L, Babini L, Mariotti S, Spada G, Abbatecola AM, Antonicelli R, Franceschi C, Procopio AD. Title Selection and validation of reference genes for studying stress-related agarwood formation of Aquilaria sinensis. Journal Plant Cell Reports. 2012. Authors Gao ZH, Wei JH, Yang Y, Zhang Z, Zhao WT. Title The vanadyl ribonucleoside complex inhibits ribosomal subunit formation in Staphylococcus aureus. Journal Journal of Antimicrobial Chemotherapy. 2012. Authors Frazier AD, Champney WS. Title Transcriptome profiling and in silico analysis of somatic embryos in Japanese larch (Larix leptolepis). Journal Plant Cell Reports. 2012. Authors Zhang Y, Zhang S, Han S, Li X, Qi L. Title Distinct immunoregulatory cytokine pattern in Egyptian patients with occult Hepatitis C infection and unexplained persistently elevated liver transaminases. Journal Asian Journal of Transfusion Science. 2012. Authors Gad YZ, Mouas N, Abdel-Aziz A, Abousmra N, Elhadidy M. Title Midkine Promotes Proliferation of Primordial Germ Cells by Inhibiting the Expression of the Deleted in Azoospermia-Like Gene. Journal Endocrinology. 2012. Authors Shen W, Park BW, Toms D, Li J. Title Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage. Journal PLOS One. 2012. Authors Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F Sala L, Morroni M, Ciarmela P, Mantovani A, Tranquilli A, Castellucci M, Marzioni D. Title Nuclear protein 1 promotes pancreatic cancer development and protects cells from stress by inhibiting apoptosis. Journal The Journal of Clinical Investigation. 2012. Authors Hamidi T, Algül H, Cano CE, Sandi MJ, Molejon MI, Riemann M, Calvo EL, Lomberk G, Dagorn JC, Weih F, Urrutia R, Schmid RM, Iovanna JL. Title Residual Expression of the Reprogramming Factors Prevents Differentiation of iPSC Generated from Human Fibroblasts and Cord Blood CD34+ Progenitors. Journal PLOS One. 2012. Authors Ramos-Mejía V, Montes R, Bueno C, Ayllón V, Real PJ, Rodríguez R, Menendez P. Title Characterization of a novel chaperone/usher fimbrial operon present on KpGI-5, a methionine tRNA gene-associated genomic island in Klebsiella pneumoniae. Journal BMC Microbiology. 2012. Authors van Aartsen JJ, Stahlhut SG, Harrison EM, Crosatti M, Ou HY, Krogfelt KA, Struve C, Rajakumar K. Title Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis. Journal Planta. 2012. Authors Zhang J and Zhang S. Title Distinct immunoregulatory cytokine pattern in Egyptian patients with occult Hepatitis C infection and unexplained persistently elevated liver transaminases. Journal Asian Journal of Transfusion Science. 2012. Authors Gad Y, Mouas N, Abdel-Aziz A, Abousmra N, Elhadidy M. Title Systematic discovery of structural elements governing stability of mammalian messenger RNAs. Journal Nature. 2012. Authors Goodarzi H, Najafabadi HS, Oikonomou P, Greco TM, Fish L, Salavati R, Cristea IM, Tavazoie S. Title Análise comparativa da região codificadora para a proteína capsidial de isolados de PepYMV e PVY coletados em pimentão. Journal Summa Phytopathologica. 2012. Authors Moura M, Marubayashi J, Mituti T, Gioria R, Kobori R, Pavan M, Krause-Sakate R. Title Identification of Conserved and Novel MicroRNAs in Aquilaria sinensis Based on Small RNA Sequencing and Transcriptome Sequence Data. Journal Gene. 2012. Authors Gao ZH, Wei JH, Yang Y, Zhang Z, Xiong HY, Zhao WT. Title M3 Subtype of Muscarinic Acetylcholine Receptor Promotes Cardioprotection via the Suppression of miR-376b-5p. Journal PLOS One. 2012. Authors Pan Z, Guo Y, Qi H, Fan K, Wang S, Zhao H, Fan Y, Xie J, Guo F, Hou Y, Wang N, Huo R, Zhang Y, Liu Y, Du Z. Title Identification of XMRV Infection-Associated microRNAs in Four Cell Types in Culture. Journal PLOS One. 2012. 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Total RNA Purification Kits Dx For the isolation and purification of total RNA from tissue samples, blood, plasma, serum, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use Note: This product is not available for sale in the United States Powered by Bioz Total RNA Purification Kits Dx For the isolation and purification of total RNA from tissue samples, blood, plasma, serum, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use Price USD$289.00 SKU Dx17200 Format Spin Column High Throughput High Throughput Deep Well Size 50 Preps Quantity