Total RNA Purification Kits

Cat. 17200, 37500, 17250, 17270, 24300, 24350, 24370, 24380

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Volume Discounts Available on Large Quantity Orders*

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What Scientists are Saying About this Product

Our RNA isolations with your test kit look very promising...We obtained large quantities of intact RNA with a 260/280 of 1.99...The other thing I should mention about the RNA kit is that we saw no DNA - which is great!

Donovan Bailey

The Walter and Eliza Hall Institute of Medical Research

We have done a comparison between your (Norgen's) Total RNA Purification kit and [the competitor] kit. The RNA was extracted from two identical samples of cultured cells (~500 000 cells per sample). As you can tell there's hardly any difference between the two kits in terms of RNA quality or quantity of small RNA species. However, Norgen kit costs half the price of the [competitor] one, takes a lot less time to run through the protocol and doesn't require additional reagents (such as Trizol). All of which makes a strong case in favour of using the Norgen kit.

The Walter and Eliza Hall Institute of Medical Research

Fred Hutchison Cancer Research Center, USA

I did try the Norgen RNA Kit and it went very well. I isolated RNA from cultured cells. I then compared it to our standard [competitor] isolation method and the Norgen kit gave more purified RNA than [competitor].

Fred Hutchison Cancer Research Center, USA

I did RNA isolation using different kits and detection afterwards microRNAs by q-PCR. Your total RNA isolation kit works better than the rest, at least in my hands.

McMaster University

…the All-in-One purification kit that [I] brought. From the same cell samples, we tested the miRNAs and the proteins and they seem to be happy with their results. In terms of miRNA purification,the total RNA extraction kit seems also to do better than other companies.

McMaster University

I just used a [competitor's] spin column to isolate some RNA and the yields were acceptable, but considerably less than what I'm used to (about 8x less). This leaves me with 2 poor yields out of 5 attempts with these columns. I was willing to chalk the first poor yield to "my error" (losing tissue or some other fluke event), but now I'm worried that the [competitor] columns are unreliable - good when they work but prone to failing from time to time... I've never had a poor yield in any of the 8 attempts I've tried with the Norgen columns.

St. Michael's Hospital

Total RNA Purification Kits

Cat. 17200, 37500, 17250, 17270, 24300, 24350, 24370, 24380

For the rapid purification of total RNA - including microRNA - without the use of phenol

Extract high quality & quantity total RNA including miRNA
No phenol step required; isolate all RNA in one fraction
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Isolate from a wide variety of specimens
CE-IVD marked version available
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Available in a variety of formats to suit your needs
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

CE-IVDR marked diagnostic version available here

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Total RNA Purification Kit
(Cat. 17200) 50 Preps

Total RNA Purification Kit
(Cat. 37500) 100 preps

Total RNA Purification Kit
(Cat. 17250) 250 preps

Total RNA Purification Kit
(Cat. 17270) 500 preps

Total RNA Purification 96-Well Kit
(Cat. 24300) 2 x 96-well plates

Total RNA Purification 96-Well Kit (Deep Well Format)
(Cat. 24350) 2 x 96-well plates

Total RNA Purification Kit 96 Well Plate Format
(Cat. 24370) 6 x 96-well plates

Total RNA Purification 96-Well Kit (Deep Well Format)
(Cat. 24380) 6 x 96-well plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

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Kit Specifications

You have selected: Cat. 17200

Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 10⁶ cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

You have selected: Cat. 37500

Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 10⁶ cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

You have selected: Cat. 17250

Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 10⁶ cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

You have selected: Cat. 17270

Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 10⁶ cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

You have selected: Cat. 24300

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:
Animal Cells	1 x 10⁶ cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 24350

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Amount of Starting Material
Animal Cells	1 x 10⁶ cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 10⁶ cells)	15μg
E. coli Cells (1 x 10⁹ cells)	50 μg

You have selected: Cat. 24370

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:
Animal Cells	1 x 10⁶ cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

You have selected: Cat. 24380

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Amount of Starting Material
Animal Cells	1 x 10⁶ cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 10⁶ cells)	15μg
E. coli Cells (1 x 10⁹ cells)	50 μg

Supporting Data

Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 10⁹ E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.

Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction. Norgen's Total RNA Purification Kit isolates the complete size range of RNA, including small RNAs without the use of phenol. Total RNA was isolated from 0.75 million HeLa cells using Norgen's Total RNA Purification Kit, various silica-based competitors and phenol-based TRI Reagent. RT-PCR was performed according to Shi and Chiang (2005). Fifteen microliters of the 50 µL isolated RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was used in a 20 µL qPCR reaction with primers against the human microRNAs (miR-19 and miR-21) and large mRNA (S15). Only the use of Norgen's Total RNA Purification Kit resulted in detection of both microRNAs and mRNA amplification products similar to Competitor 2's kit but without the use of phenol.

Figure 3. High Quality of RNA from a Diverse Range of Inputs. Norgen's Total RNA Purification Kit allows RNA isolation from a wide range of species or tissue types. Total RNA was isolated from 5 x 10⁸ E. coli cells, 1 x 10⁶ HeLa cells, 100 µL rat blood and 10 mg hamster kidney using Norgen's Total RNA Purification Kit. One microliter of the 50 µL isolated RNA was analyzed on the Agilent® 2100 BioAnalyzer using an RNA Nano 6000 chip. Note the integrity of RNA from all inputs with the presence of small RNA species. Norgen's Total RNA Purification Kit consistently isolates high quality RNA from various inputs that score a RIN value between 8 and 10.

Figure 4. Great Isolation Sensitivity. Norgen's Total RNA Purification Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of 293 HEK cells from 1 million cells down to a single cell. Five microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL PCR reaction with primers to detect the human beta-actin transcripts. PCR products of beta-actin were detected from as little as a single cell. M is the marker lane.

Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA. Norgens Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification had the best linearity (higher R²) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21).

Figure 6. Effective and Consistent Detection of miRNA from Plasma. Norgen's Total RNA Purification Kit can effectively isolate miRNA from plasma. Total RNA was isolated from 50, 100 or 200 µL of rat plasma in triplicates using Norgen's Total RNA Purification Kit (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Stem loop RT-qPCR using primers specific to miR-21 was performed. In brief, two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21. Norgen's Total RNA Purification Kit is the only product that showed (1) consistent detection of miR-21 transcripts across all input volumes, and (2) Ct values correlated to input volume (decrease Ct with increase input).

Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency. Norgen's Total RNA Purification Kit isolates plasma RNA that performs more consistently in downstream applications such as microarrays. Total RNA including miRNA was isolated from 100 µL of mouse plasma in duplicate using Norgen's Total RNA Purification Kit, Competitor 1's leading miRNA Kit or a phenol-based RNA extraction method. One hundred nanograms of extracted RNA from each kit was applied to an Illumina microRNA expression profiling kit. Scatter plots display better consistency (better clustering) of replicate signals from Norgen's samples. Genes with Pval < 0.01 for both replicates were in blue. The associated table suggested that Norgen's protocol recovered the same diversity of miRNA with better consistency (higher r² value).

Figure 8. Better Diversity of miRNA Detected from Plasma. Norgen's Total RNA Purification Kit isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit or 625 µL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitors miRNA kit (right). Image courtesy of LC Sciences, Houston.

Figure 9. Better Diversity of miRNA Detected from HeLa Cells using Illumina Small RNA Next Gen Sequencing.Norgen's Total RNA Purification Kit isolates miRNA from HeLa cells with better diversity than a leading competitor. Total RNA including miRNA was isolated from 1 million HeLa cells using Norgen's Total RNA Purification Kit and Competitor Q's leading miRNA Kit, and was applied to Illumina Small RNA Next Gen Sequencing on a MiSeq sequencer. Panel A showed that Norgens Total RNA Purification Kit recovers ahigher number of miRNAsthan the competitor. In particular, the higher diversity is achieved with a faster and simpler procedure in as little as 20 minutes of RNA sample preparation time without the use of phenol. Panel B is a scatter plot of the average RPM (reads per million) of the miRNAs detected used to compare Norgen and the competitorskit recovery of miRNA. Norgens Total RNA Purification Kit recovered a significantly higher number of miRNAs that have higher RPM, as summarized in the graph insert as well as in Panel C.

Figure 10. Consistent Yield of High Quality RNA with Complete Size Range without the Use of Phenol. Norgen's Total RNA Purification 96-Well Kit allows for the consistent isolation of high quality RNA, with complete sizes ranging from very large RNA down to small RNA without the use of phenol. Total RNA was isolated from 0.5 mL aliquots of an overnight culture of E. coli (~5 x 10⁸ cells/mL) using Norgen's Total RNA Purification 96-Well Kit in 16 replicates. Five microliters of the 75 µL isolated RNA were resolved on a 1.2% formaldehyde agarose gel. All 16 replicates showed both high yield and high quality. In addition, all replicates showed effective recovery of all sizes of RNA including the small RNA (arrow).

Figure 11. High Quality of RNA from a Diverse Range of Inputs. Norgen's Total RNA Purification 96-Well Kit allows for RNA isolation from a wide range of species and tissue types. Total RNA was isolated from 8 mg of brain (Lanes A), kidney (Lanes B), liver (Lanes C), lung (Lanes D) and spleen tissue (Lanes E), 7.5 x 10⁵ HeLa (Lanes F) and CHO cells (Lanes G), and 5 x 10⁸ bacteria (Lanes H) using Norgen's Total RNA Purification 96-Well Kit. Five microliters of the 75 µL isolated RNA were resolved on a 1.2% formaldehyde agarose gel. From observing the formaldehyde-agarose gel, it can be seen that Norgen's kit can be used to successfully isolate total RNA, including small RNA species, from a broad range of sample types.

Figure 12. Isolation of Both Large and Small RNA. Norgen's Total RNA Purification 96-Well Kit allows for consistent isolation of both large and small RNA. Total RNA was isolated from samples of 5 x 10⁵ HeLa cells using Norgen's Total RNA Purification 96-Well Kit. Aliquots of each total RNA sample were then used in 2 different RT-qPCR reactions. Ten microliters of the 75 µL isolated RNA were subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. Three microliters of the reverse transcription were used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15 transcripts (Panel B), respectively. Both the mRNA and microRNA were amplified in a consistent manner from all the samples, with low variability of the Ct values. Thus both types of RNA are being consistently isolated using this kit.

Figure 13. Great Isolation Sensitivity. Norgen's Total RNA Purification Kit allows for sensitive RNA extraction from even less than 10 cells. RT-qPCR was used to detect mRNA isolated from various input amounts of HeLa cells, from 100,000 down to a single cell, using Norgen's Total RNA Purification 96-Well Kit. Ten microliters of the 75 µL isolated RNA were then subjected to a 20 µL reverse transcription using oligo dT primers. Three microliters of the reverse transcription were used in a 20 µL real-time PCR reaction with primers to detect the human S15 transcripts. The resulting Ct values were plotted against the input cell number. Total RNA was isolated and detected linearly from as little as a single HeLa cell.

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Protocols:

(17200, 37500, 17250, 17270) Total RNA Purification Kit (Spin Column) - Protocol (50, 100, 250 and 500 prep)

(24300, 24370) Total RNA Purification 96-Well Kit (High Throughput) - Protocol (96-well)

(24350, 24380) Total RNA Purification 96-Well Kit (High Throughput Deep Well) - Protocol (Deep Well)

Supplementary Protocols:

Supplementary Protocol - Total RNA Isolation From Cerebrospinal Fluid Csf

Total RNA Purification Kit - Supplementary Protocol for Exosomal RNA Purification from Exosomes Already Purified

Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacterial Species

Isolation of RNA from Norgen’s Swab Collection and Total Nucleic Acid Preservation System

Supplementary Protocol - Norgen's Saliva RNA Collection and Preservation Devices

Supplementary Protocol for Isolating Viral RNA from Swab Samples

Application Notes:

Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen’s Total RNA Purification Kit

Isolation of True Total RNA using the Tempus™ Blood RNA Tube and Norgen’s Total RNA Purification Kit

Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study

Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input

Effect of microRNA GC Content On RNA Purification Efficiency

Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device

Effect of microRNA GC Content on RNA Purification Efficiency

Safety Data Sheets:

(17200, 37500, 17250) Total RNA Purification Kit - Safety Data Sheet

(24300) Total RNA Purification 96-Well Kit - Safety Data Sheet

Posters:

RNA Quality Assessment and The Effect On Gene Expression Studies - ASCB 2008

Substrate Specificity of Taq Polymerase - ASCB 2007

Genomic Profiling of Hepatitis B Virus and Hepatitis C Virus From 1 mL of Urine - ASM 2007

Silicon Carbide As A Novel RNA Affinity Medium With Improved Sensitivity and Size Diversity - microRNA 2008

Effect of RNA Isolation Methods On microRNA Quantity and Quality In Plasma - TriCon 2013

Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - ASCB 2008

Variation Between The Purification Efficiency of microRNA With Different Gc Content - TriCon 2013

The Impact of Sample Preparation On Microrna Quality and Diversity For Downstream Applications - microRNA 2009

Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system

Product Flyers:

Total RNA Purification

RNA Clean-Up and Concentration Micro-Elute Kit

Spin Column (17200, 37500, 17250, 17270)

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Title	Antifungal Effect of Copper Nanoparticles against Fusarium kuroshium, an Obligate Symbiont of Euwallacea kuroshio Ambrosia Beetle
Journal	J. Fungi. 2022
Authors	Enrique Ibarra-Laclette, Jazmín Blaz, Claudia-Anahí Pérez-Torres, Emanuel Villafán, Araceli Lamelas, Greta Rosas-Saito, Luis Arturo Ibarra-Juárez, Clemente de Jesús García-Ávila, Arturo Isaías Martínez-Enriquez and Nicolaza Pariona

Title	Environmental viromes reveal global virosphere of deep-sea RNA viruses
Journal	Research Square. 2022
Authors	Xiaobo Zhang

Title	Circular RNA hsa_circ_0119412 contributes to tumorigenesis of gastric cancer via the regulation of the miR-1298-5p/zinc finger BED-type containing 3 (ZBED3) axis
Journal	Bioengineered. 2022
Authors	Ting Huang, Yacheng Wang, Miao Li, Wenjie Wang, Zhaozhen Qi & Jun Li

Title	Thyroid hormones regulate cardiac repolarization and QT-interval related gene expression in hiPSC cardiomyocytes
Journal	Scientific Reports. 2022
Authors	Alessandra Ulivieri, Luca Lavra, Fiorenza Magi, Alessandra Morgante, Leonardo Calò, Patrizio Polisca, Leila B. Salehi & Salvatore Sciacchitano

Title	C/EBPB-dependent adaptation to palmitic acid promotes tumor formation in hormone receptor negative breast cancer
Journal	Nature Communications. 2022
Authors	Xiao-Zheng Liu, Anastasiia Rulina, Man Hung Choi, Line Pedersen, Johanna Lepland, Sina T. Takle, Noelly Madeleine, Stacey D’mello Peters, Cara Ellen Wogsland, Sturla Magnus Grøndal, James B. Lorens, Hani Goodarzi, Per E. Lønning, Stian Knappskog, Anders Molven & Nils Halberg

Title	Mitochondrial DNA in Visceral Adipose Tissue in Severe Obesity: From Copy Number to D-Loop Methylation
Journal	Front. Biosci. 2022.
Authors	Laura Bordoni, Jessica Perugini, Irene Petracci, Eleonora Di Mercurio, Giovanni Lezoche, Mario Guerrieri, Antonio Giordano, Rosita Gabbianelli

Title	A Novel Probiotic Bacillus subtilis Strain Confers Cytoprotection to Host Pig Intestinal Epithelial Cells during Enterotoxic Escherichia coli Infection
Journal	Environmental Microbiology. 2022.
Authors	Sudhanshu Sudan, Xiaoshu Zhan, Julang Li

Title	Circular RNA hsa_circ_0057452 facilitates keloid progression by targeting the microRNA-1225-3p/AF4/FMR2 family member 4 axis
Journal	Bioengineered. 2022.
Authors	Hu Gao, Zhen Hu & Xiangming Zhang

Title	Identification of gene family members and a key structural variation reveal important roles of OVATE genes in regulating tea (Camellia sinensis) leaf development
Journal	Front. Plant Sci. 2022.
Authors	Yanlin An, Xiaobo Xia, Tingting Jing and Feng Zhang

Title	The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus
Journal	Antioxidants. 2022.
Authors	Francesco Piarulli, Cristina Banfi, Maura Brioschi, Alessandra Altomare, Eugenio Ragazzi, Chiara Cosma, Giovanni Sartore and Annunziata Lapolla

Title	Prevalence of SARS-CoV-2 infection in the workplace: results of a year of investigation in the Marche Nord companies
Journal	Acta Biomed. 2022.
Authors	Chiara Orlandi, Chiara Orlandi, Giuditta Fiorella Schiavano, Giorgio Brandi, Mauro Magnani, Anna Casabianca

Title	Circulating plasma microRNAs in systemic sclerosis-associated pulmonary arterial hypertension
Journal	Rheumatology, Volume 61, Issue 1. 2022.
Authors	Dirk M Wuttge, Anting L Carlsen, Gabriel Teku, Marie Wildt, Göran Rådegran, Mauno Vihinen, Niels H H Heegaard, Roger Hesselstrand

Title	SARS-CoV-2 modulates virus receptor expression in placenta and can induce trophoblast fusion, inflammation and endothelial permeability
Journal	Front Immunol. 2022.
Authors	Chiara Agostinis, Miriam Toffoli, Mariagiulia Spazzapan, Andrea Balduit, Gabriella Zito, Alessandro Mangogna, Luisa Zupin, Tiziana Salviato, Serena Maiocchi, Federico Romano, Sergio Crovella, Francesco Fontana, Luca Braga, Marco Confalonieri, Giuseppe Ricci, Uday Kishore, Roberta Bulla

Title	Randomized, Double-Blind, Placebo-Controlled Trial to Test the Effects of a Nutraceutical Combination Monacolin K-Free on the Lipid and Inflammatory Profile of Subjects with Hypercholesterolemia
Journal	Nutrients. 2022.
Authors	Olga Protic, Raffaele Di Pillo, Alberto Montesanto, Roberta Galeazzi, Giulia Matacchione, Angelica Giuliani, Jacopo Sabbatinelli, Felicia Gurău, Andrea Silvestrini, Fabiola Olivieri, Roberto Antonicelli and Anna Rita Bonfigli

Title	SIRT3 Modulates Endothelial Mitochondrial Redox State during Insulin Resistance
Journal	Antioxidants. 2022.
Authors	Elisa Martino, Anna Balestrieri, Camilla Anastasio, Martina Maione, Luigi Mele, Domenico Cautela, Giuseppe Campanile, Maria Luisa Balestrieri and Nunzia D’Onofrio

Title	MicroRNA-574 Impacts Granulosa Cell Estradiol Production via Targeting TIMP3 and ERK1/2 Signaling Pathway
Journal	Front Endocrinol (Lausanne). 2022.
Authors	Bo Pan, Xiaoshu Zhan, Julang Li

Title	Curcumin, Polydatin and Quercetin Synergistic Activity Protects from High-Glucose-Induced Inflammation and Oxidative Stress
Journal	Antioxidants. 2022.
Authors	Giulia Matacchione, Debora Valli, Andrea Silvestrini, Angelica Giuliani, Jacopo Sabbatinel, Chiara Giordani, Sofia Coppari, Maria Rita Rippo, Maria Cristina Albertini and Fabiola Olivieri

Title	20(S)- Protopanaxadiol suppresses hepatic stellate cell activation via WIF1 demethylation-mediated inactivation of the Wnt/β-catenin pathway
Journal	Journal of Ginseng Research. 2022.
Authors	Chunxue Li, Yating Zhan, Rongrong Zhang, Qiqi Tao, Zhichao Lang, Jianjian Zheng

Title	Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome
Journal	bioRxiv. 2022.
Authors	Cristina Morsiani, Maria Giulia Bacalini, Salvatore Collura, María Moreno-Villanueva, Nicolle Breusing, Alexander Bürkle, Tilman Grune, Claudio Franceschi, Magda De Eguileor, Miriam Capri

Title	A coordinated function of lncRNA HOTTIP and miRNA-196b underpinning leukemogenesis by targeting FAS signaling
Journal	bioRxiv. 2022.
Authors	Ajeet P. Singh, Huacheng Luo, Meghana Matur, Melanie Eshelman, Arati Sharma, Suming Huang

Title	Senescent macrophages in the human adipose tissue as a source of inflammaging
Journal	GeroScience. 2022.
Authors	Giulia Matacchione, Jessica Perugini, Eleonora Di Mercurio, Jacopo Sabbatinelli, Francesco Prattichizzo, Martina Senzacqua, Gianluca Storci, Christian Dani, Giovanni Lezoche, Mario Guerrieri, Antonio Giordano, Massimiliano Bonafè & Fabiola Olivieri

Title	Investigation of the direct effect of a precision Ascophyllum nodosum biostimulant on nitrogen use efficiency in wheat seedlings
Journal	Plant Physiology and Biochemistry 2022.
Authors	Łukasz Łangowski, Oscar Goñi, Elomofe Ikuyinminu, Ewan Feeney, Shane O' Connell

Title	Ca2+ dysregulation in cardiac stromal cells sustains fibro-adipose remodeling in Arrhythmogenic Cardiomyopathy and can be modulated by flecainide
Journal	Journal of Translational Medicine 2022.
Authors	Angela S. Maione, Pawan Faris, Lara Iengo, Valentina Catto, Luca Bisonni, Francesco Lodola, Sharon Negri, Michela Casella, Anna Guarino, Gianluca Polvani, Marina Cerrone, Claudio Tondo, Giulio Pompilio, Elena Sommariva & Francesco Moccia

Title	KDM2A and KDM3B as Potential Targets for the Rescue of F508del-CFTR
Journal	Int. J. Mol. Sci. 2022.
Authors	Claudio D’Amore, Christian Borgo, Valentina Bosello Travain and Mauro Salvi

Title	Efficacy of antiseptic mouthrinses against SARS-CoV-2: A prospective randomized placebo-controlled pilot study
Journal	American Journal of Otolaryngology. 2022.
Authors	Paolo Junior Fantozzia, Emanuele Pampena, Alessandra Pierangeli, Giuseppe Oliveto, Leonardo Sorrentino, Domenico Di Vanna, Riccardo Pampena, Alessandro Lazzaro, Elio Gentilini, Claudio Maria Mastroianni, Gabriella D'Ettorre, Antonella Polimeni, Umberto Romeo, Alessandro Villa

Title	Contributions of Circulating microRNAs for Early Detection of Lung Cancer
Journal	Cancers. 2022.
Authors	Jody Vykoukal, Johannes F. Fahrmann, Nikul Patel, Masayoshi Shimizu, Edwin J. Ostrin, Jennifer B. Dennison, Cristina Ivan, Gary E. Goodman, Mark D. Thornquist, Matt J. Barnett, Ziding Feng, George A. Calin and Samir M. Hanash

Title	A Possible Cause for the Differential Expression of a Subset of miRNAs in Mesenchymal Stem Cells Derived from Myometrium and Leiomyoma
Journal	Genes. 2022.
Authors	Mariangela Di Vincenzo, Concetta De Quattro, Marzia Rossato, Raffaella Lazzarini, Giovanni Delli Carpini, Andrea Ciavattini and Monia Orciani

Title	Quantitation of neuroxin-1, ataxin-3 and atlastin genes related to grooming behavior in five races of honey bee, Apis mellifera L., 1758 (Hymenoptera: Apidae), in Turkey
Journal	Türk. entomol. derg. 2022.
Authors	Berkant İsmail YILDIZ and Kemal KARABAĞ

Title	Airborne concentrations of SARS-CoV-2 in indoor community environments in Italy
Journal	Environmental Science and Pollution Research. 2022.
Authors	Marianna Conte, Matteo Feltracco, Daniela Chirizzi, Sara Trabucco, Adelaide Dinoi, Elena Gregoris, Elena Barbaro, Gianfranco La Bella, Giuseppina Ciccarese, Franco Belosi, Giovanna La Salandra, Andrea Gambaro & Daniele Contini

Title	microRNA-1 Induces Transdifferentiation of Peripheral Blood CD34+ Cells into Cardiomyocytes-like Cells
Journal	The Indonesian Biomedical Journal. 2022.
Authors	Budi Susetio Pikir, Andrianto, I Gde Rurus Suryawan, Hanestya Oky Hermawan, Dian Paramita Kartikasari, Primasitha Maharany Harsoyo

Title	Novel Splicing Mutation in MTM1 Leading to Two Abnormal Transcripts Causes Severe Myotubular Myopathy
Journal	Int. J. Mol. Sci. 2022.
Authors	Luca Bosco, Daniela Leone, Laura Costa Comellas, Mauro Monforte, Marika Pane, Eugenio Mercuri, Enrico Bertini, Adele D’Amico and Fabiana Fattori

Title	DNAJB1-PRKACA in HEK293T cells induces LINC00473 overexpression that depends on PKA signaling
Journal	PLOS ONE 2022.
Authors	Stephanie S. Kim, Ina Kycia, Michael Karski, Rosanna K. Ma, Evan A. Bordt, Julian Kwan, Anju Karki, Elle Winter, Ranan G. Aktas, Yuxuan Wu, Andrew Emili, Daniel E. Bauer, Praveen Sethupathy, Khashayar Vakili

Title	Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model
Journal	bioRxiv. 2022.
Authors	Yu-Han Hung, Meghan Capeling, Jonathan W. Villanueva, Matt Kanke, Michael T. Shanahan, Sha Huang, Rebecca L. Cubitt, Vera D. Rinaldi, John C. Schimenti, Jason R. Spence, Praveen Sethupathy

Title	Failure of human rhombic lip differentiation underlies medulloblastoma formation
Journal	Nature. 2022.
Authors	Liam D. Hendrikse, Parthiv Haldipur, Olivier Saulnier, Jake Millman, Alexandria H. Sjoboen, Anders W. Erickson, Winnie Ong, Victor Gordon, Ludivine Coudière-Morrison, Audrey L. Mercier, Mohammad Shokouhian, Raúl A. Suárez, Michelle Ly, Stephanie Borlase, David S. Scott, Maria C. Vladoiu, Hamza Farooq, et al.

Title	MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells
Journal	AMERICAN JOURNAL OF PHYSIOLOGY. 2022.
Authors	Basak Icli, Hao Li, Daniel Pérez-Cremades, Winona Wu, Denizhan Ozdemir, Stefan Haemmig, Raphael Boesch Guimaraes, Andre Manica, Julio F. Marchini, Dennis P. Orgill, and Mark W. Feinberg

Title	Novel candidates for chronic diabetic wound healing
Journal	Journal of Pakistan Association of Dermatologists. 2022.
Authors	Muhammad Shakil Khan, Tauqeer Ahmed Malik

Title	Validation of Microchip Based RT-PCR ABC Test (InfA/B & COVID-19) in Clinical Samples
Journal	Journal of Biosciences and Medicines 2022.
Authors	Gabriel Martinez, Ryan Nunley, Michelle Gaines, Timea Majoros, Rajwant K. Gill, Irina Gelimson, Natallia Varankovich, Maxim Slyadnev, Sikander S. Gill

Title	Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45–50 into an induced pluripotent stem cell line (CCMi005-A)
Journal	Stem Cell Research 2022.
Authors	Davide Rovina, Elisa Castiglioni, Sara Mallia, Martina Rabino, Andrea Farini, Marzia Belicchi, Giusy Di Giuseppe, Cristina Gervasini, Yvan Torrente, Giulio Pompilio, Aoife Gowran

Title	Allatotropin in the Corpora Allata and Ovaries of Rhodnius prolixus: Probable in situ regulatory mechanisms
Journal	Allatotropin in Rhodnius prolixus 2022.
Authors	Villalobos Sambucaro, María José, Ronderos, Jorge Rafael

Title	SARS-CoV-2 RNA Detection on Environmental Surfaces in a University Setting of Central Italy
Journal	Int. J. Environ. Res. Public Health 2022.
Authors	Anna Casabianca, Chiara Orlandi, Giulia Amagliani, Mauro Magnani, Giorgio Brandi and Giuditta Fiorella Schiavano

Title	Interferon Tau (IFNt) and Interferon-Stimulated Genes (ISGs) Expression in Peripheral Blood Leukocytes and Correlation with Circulating Pregnancy-Associated Glycoproteins (PAGs) during Peri-Implantation and Early Pregnancy in Buffalo Cows
Journal	Animals 2022.
Authors	Anna Beatrice Casano, Vittoria Lucia Barile, Laura Menchetti, Gabriella Guelfi, Gabriele Brecchia, Stella Agradi, Giovanna De Matteis, Maria Carmela Scatà, Francesco Grandoni and Olimpia Barbato

Title	Early Life Social Stress Causes Sex- and Region-Dependent Dopaminergic Changes that Are Prevented by Minocycline
Journal	Molecular Neurobiology. 2022.
Authors	Clarissa Catale, Luisa Lo Iacono, Alessandro Martini, Constantin Heil, Ezia Guatteo, Nicola Biagio Mercuri, Maria Teresa Viscomi, Daniela Palacios & Valeria Carola

Title	Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus
Journal	Research Square 2022.
Authors	Ojo, Stephen Kayode .S, Udewena, Linda Uchenna, Adetunji, Charles O

Title	Functional genetic screen identifies ITPR3/calcium/ RELB axis as a driver of colorectal cancer metastatic liver colonization
Journal	Developmental Cell 2022.
Authors	Ryan H. Moy, Alexander Nguyen, Jia Min Loo, Y. Gloria Wu, Saeed Tavazoie, Sohail F. Tavazoie

Title	Multiomic analysis of microRNA-mediated regulation reveals a proliferative axis involving miR-10b in fibrolamellar carcinoma
Journal	JCI insight 2022.
Authors	Adam B. Francisco, Matt Kanke, Andrew P. Massa, Timothy A. Dinh, Ramja Sritharan, Khashayar Vakili, Nabeel Bardeesy, and Praveen Sethupathy

Title	Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth
Journal	Host-Microbial Inter

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Total RNA Purification Kits (Cat. 17200, 37500, 17250, 17270, 24300, 24350, 24370, 24380)

Total RNA Purification Kits

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Total RNA Purification Kits

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Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

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Total RNA Purification Kits (Cat. 17200, 37500, 17250, 17270, 24300, 24350, 24370, 24380)

Total RNA Purification Kits

What Scientists are Saying About this Product

Total RNA Purification Kits

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Request a Product Sample

Product Overview

Documentation

Citations

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.