Our RNA isolations with your test kit look very promising...We obtained large quantities of intact RNA with a 260/280 of 1.99...The other thing I should mention about the RNA kit is that we saw no DNA - which is great!
We have done a comparison between your (Norgen's) Total RNA Purification kit and [the competitor] kit. The RNA was extracted from two identical samples of cultured cells (~500 000 cells per sample). As you can tell there's hardly any difference between the two kits in terms of RNA quality or quantity of small RNA species. However, Norgen kit costs half the price of the [competitor] one, takes a lot less time to run through the protocol and doesn't require additional reagents (such as Trizol). All of which makes a strong case in favour of using the Norgen kit.
The Walter and Eliza Hall Institute of Medical Research
I did try the Norgen RNA Kit and it went very well. I isolated RNA from cultured cells. I then compared it to our standard [competitor] isolation method and the Norgen kit gave more purified RNA than [competitor].
Fred Hutchison Cancer Research Center, USA
I did RNA isolation using different kits and detection afterwards microRNAs by q-PCR. Your total RNA isolation kit works better than the rest, at least in my hands.
…the All-in-One purification kit that [I] brought. From the same cell samples, we tested the miRNAs and the proteins and they seem to be happy with their results. In terms of miRNA purification,the total RNA extraction kit seems also to do better than other companies.
I just used a [competitor's] spin column to isolate some RNA and the yields were acceptable, but considerably less than what I'm used to (about 8x less). This leaves me with 2 poor yields out of 5 attempts with these columns. I was willing to chalk the first poor yield to "my error" (losing tissue or some other fluke event), but now I'm worried that the [competitor] columns are unreliable - good when they work but prone to failing from time to time... I've never had a poor yield in any of the 8 attempts I've tried with the Norgen columns.
St. Michael's Hospital
Total RNA Purification Kits
For the rapid purification of total RNA - including microRNA - without the use of phenol
For research use only and NOT intended for in vitro diagnostics.
Total RNA Purification 96-Well Kit (Cat. 24300)2 x 96-well plates
Total RNA Purification 96-Well Kit (Deep Well Format) (Cat. 24350)2 x 96-well plates
Total RNA Purification Kit 96 Well Plate Format (Cat. 24370)6 x 96-well plates
Total RNA Purification 96-Well Kit (Deep Well Format) (Cat. 24380)6 x 96-well plates
Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.