Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) Note: This product is not available for sale in the United States Powered by Bioz Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) SKU Dx42800 Format Slurry Size 50 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma/serum samples for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation. Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay. Détails Supporting Data Previous Figure 1. Isolation and Detection of Circulating RNA from 1 mL Samples of Serum and Plasma Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) was used to isolate circulating RNA from 1 mL samples of serum and plasma. Five microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene and the miR-21 gene. Both the 5S housekeeping gene and the miR-21 gene could be detected from plasma (red) and serum (green), indicating the high quality of the purified RNA. The black line corresponds to the no template control. Figure 2. Effective and Consistent Detection of Plasma Exosome RNA. Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kits can effectively isolate RNA from plasma. Plasma Exosome RNA was isolated from 500 µL of human plasma prepared from blood collected on citrate, EDTA or Heparin in triplicates using Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kit Dx (blue), a ExoQuick Exosome Precipitation Reagent (green) and Qiagen’s QIAzol extraction followed by a modified Qiagen’s RNeasy Mini Kit cleanup (red). Stem loop RT-qPCR using primers specific to miR-21 and miR-16 as well as the housekeeping 5S rRNA was performed. In brief, three microliters of the 100 µL isolated RNA was then subjected to a 20 µL reverse transcription using 5S rRNA, miR-21 and miR-16 stem-loop reverse primer or reveres primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21, human miR-16 and the 5S rRNA. Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kit Dx is the only product that showed consistent detection of all tested transcripts with the highest quality regardless the type of the anti-coagulant used for blood collection. Next Click for expanded view Kit Specifications Minimum Plasma/Serum Input 0.25 mL Maximum Plasma/Serum Input 5 mL Size of RNA Purified All sizes, including microRNA Time to Complete 10 Purifications < 40 minutes Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed. Component Cat. Dx42800 (50 preps) PS Solution A 12 mL PS Solution B 100 mL PS Solution C 18 mL Wash Solution 22 mL RNA Elution Solution 6 mL Mini Filter Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (Dx42800) Plasma/Serum Circulating RNA and Exosomal Purification Kit Dx (Slurry Format) - Protocol (50 preps) SUPPLEMENTARY PROTOCOLS Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) - Supplementary Protocol PRODUCT INFORMATION SHEETS Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) - Product Information Sheet APPLICATION NOTES Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) Effect of Microrna Gc Content On RNA Purification Efficiency - Application Note SAFETY DATA SHEETS (Dx42800) Plasma/Serum Circulating RNA and Exosomal Purification Kit Dx (Slurry Format) - Safety Data Sheet RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Related Services NGS RNA Isolation Related Products Blood DNA Isolation Mini Kit RNase-Free DNase I Kit Total RNA Purification 96-Well Kit Plasma/Serum Exosome Purification and RNA Isolation Mini Kit Urine Exosome Purification and RNA Isolation Mini Kit
Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) Note: This product is not available for sale in the United States Powered by Bioz Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) SKU Dx42800 Format Slurry Size 50 Preps Quantity Trouver un distributeur Contact