Total RNA Purification Micro Kit
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For research use only and NOT intended for in vitro diagnostics.
Aperçu du produit
This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications.
This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
---|---|---|---|---|---|
55000 | Plasma/Serum RNA Purification Mini Kit | 10 - 25 µL | 50 µL - 1 mL | 250 µL - 1 mL | 5 - 10 mL |
35300 | Total RNA Purification Micro Kit | 20 - 50 µL | 1 - 4 mL | 2 - 10 mL | 10 - 20 mL |
17200 | Total RNA Purification Kit | 50 - 100 µL | 4 - 10 mL | 11 - 30 mL | 20 - 35 mL |
Détails
Supporting Data
Figure 1. Great Isolation Sensitivity. Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells. Five µL of the eluted RNA was used as the template in a 20 µL RT-qPCR reaction to detect the human S14 transcript. The S14 was detected from as little as a single cell.
Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM). Norgen's Total RNA Purification Micro Kit allows sensitive but high quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen's Total RNA Purification Micro Kit recovered good amounts of RNA with excellent 260/280 ratio.
Figure 3. Better Efficiency of RNA Recovery at Single Cell Input. Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor's kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen's Total RNA Purification Micro Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript transcript in the isolated RNA. RNA isolated by Norgen's Total RNA Purification Micro Kit showed much higher relative expression of each RNA transcript tested at all cell input number down to single cell.
Kit Specifications
|
|
Maximum Column Binding Capacity
|
35 μg
|
Maximum Column Loading Volume
|
650 μL
|
Minimum Elution Volume |
20 μL
|
Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) |
5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells |
Time to Complete 10 Purifications |
20 minutes
|
Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 35300 (50 preps) | Cat. 35350 (250 preps) |
---|---|---|
Buffer RL | 40 mL | 5 x 40 mL |
Wash Solution A | 38 mL | 5 x 38 mL |
Elution Solution A | 6 mL | 5 x 6 mL |
Micro Spin Columns | 50 | 250 |
Collection Tubes | 50 | 250 |
Elution Tubes (1.7 mL) | 50 | 250 |
Product Insert | 1 | 1 |