COVID-19 TaqMan RT-PCR Detection Kit (N/ORF1ab genes) (Cat. TM67300, TM67320)
COVID-19 TaqMan RT-PCR Detection Kit (N/ORF1ab genes)
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COVID-19 TaqMan RT-PCR Detection Kit (N/ORF1ab genes)
- Multiplex RT-PCR system detects two SARS-CoV-2 specific genes (N gene and ORF1ab) in a single one step RT-PCR reaction
- Primer & Probe Mixes contain all N gene, ORF1ab and human RP assays
- Positive Control contains the three SARS-related target genes: N gene, ORF1ab gene as well as the human RP (internal control).
- Convenient ready-to-use 2X One-Step RT-PCR Master Mix
- Manufactured under ISO 13485:2016 for research use only
- CE-IVD marked version also available
- Find out more information about our complete COVID-19 workflow
For research use only and NOT intended for in vitro diagnostics.
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(Cat. TM67300) 500 Reactions
(Cat. TM67320) 50 Reactions
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 6 months at -20°C without showing any reduction in performance.
Supporting Data
COVID-19 TaqMan RT-PCR Detection Kit (N/ORF1ab genes)
Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains three SARS-CoV-2 detection targets; N (Nucleoprotein),   ORF1ab (Open reading frame1ab) and the human RNase P transcript (RP - HEX/VIC) as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/ORF1ab/RP Positive Control contains an in vitro RNA transcript for the two SARS-related target genes: N gene, ORF1ab gene as well as the human RP gene (internal control).
This kit was developed and validated to be used with the following PCR instruments:
- Qiagen Rotor-Gene Q, BioRad CFX96 Touchâ„¢ Real-Time PCR Detection System, ABI 7500, ABI Quantstudio 5 and 7.
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Supporting Data
Kit Specifications
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 6 months at -20°C without showing any reduction in performance.
