|Exosomal RNA Isolation Kit||58000||50 prep|
Exosomal RNA Isolation Kit
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from exosomes previously purified using Norgen's Urine Exosome Purification Kits or Norgen's Plasma/Serum Exosome Purification Kits. This kit also allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Plasma/Serum or Urine Exosomes
purified using Norgen’s Urine Exosome Purification Kits
or Plasma/Serum Exosome Purification Kits
|Size of RNA Purified||
All sizes, including miRNA
and small RNA (< 200 nt)
|Time to Complete 10 Purifications||
Variable depending on specimen
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
|Title||Extracellular microRNA signature in chronic kidney disease|
|Journal||American Journal of Physiology. 2017.|
|Authors||Muralidharan J, Ramezani A, Hubal MJ, Knoblach S, Shrivastav S, Karandish S, Scott R, Maxwell N, Ozturk S, Beddhu S, Kopp JB, Raj DS.|
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