Exosomal RNA Isolation Kit (Cat. 58000)

This kit is for use with Norgen's exosome purification technology only. Click here to view more.

  • Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
  • ​Bind and elute all RNA irrespective of size or GC content, without bias
  • No phenol extractions
  • No Proteinase K treatment
  • No carrier RNA
  • Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
  • Purify high-quality RNA in 15-20 minutes
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

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ItemsCat. #Size
Exosomal RNA Isolation Kit 58000 50 prep

Exosomal RNA Isolation Kit

This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from exosomes previously purified using Norgen's Urine Exosome Purification Kits or Norgen's Plasma/Serum Exosome Purification Kits. This kit also allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.

The Exosomal RNA Isolation Kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Sample type
Plasma/Serum or Urine Exosomes
purified using Norgen’s Urine Exosome Purification Kits
or Plasma/Serum Exosome Purification Kits
Size of RNA Purified
All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Protocols: 
58000 - Protocol (50 prep)
Product Information Sheets: 
58000 - Product Information Sheet
Safety Data Sheets: 
58000 - Safety Data Sheet

Title Extracellular microRNA signature in chronic kidney disease
Journal American Journal of Physiology. 2017.
Authors Muralidharan J, Ramezani A, Hubal MJ, Knoblach S, Shrivastav S, Karandish S, Scott R, Maxwell N, Ozturk S, Beddhu S, Kopp JB, Raj DS.