Exosomal RNA Isolation Kit (Cat. 58000)
Exosomal RNA Isolation Kit
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Exosomal RNA Isolation Kit
All-in-one system for the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits
- Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- No phenol extractions
- No Proteinase K treatment
- No carrier RNA
- Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
- Purify high-quality RNA in 15-20 minutes
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
This kit is for use with Norgen's exosome purification technology only. Click here to view more.
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(Cat. 58000) Variable
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- Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- No phenol extractions
- No Proteinase K treatment
- No carrier RNA
- Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
- Purify high-quality RNA in 15-20 minutes
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Exosomal RNA Isolation Kit
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits. This kit also allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
How to Isolate Exosomal RNA From Exosomes
Kit Specifications
|
Kit Specifications
|
|
|
Sample type
|
Exosomes
purified using Norgen’s Purification Kits |
| Size of RNA Purified |
All sizes, including miRNA
and small RNA (< 200 nt) |
| Elution Volume |
50-100 μL
|
| Time to Complete 10 Purifications |
35-40 minutes
|
| Average Yields* |
Variable depending on specimen
|
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Supporting Data
| Component | Cat. 58000 (50 preps) |
|---|---|
| Lysis Buffer A | 20 mL |
| Lysis Additive B | 2 mL |
| Wash Solution A | 18 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Biological substrate modification suppresses ventricular arrhythmias in a porcine model of chronic ischaemic cardiomyopathy |
| Journal | Eur Heart J. 2022. |
| Authors | James F. Dawkins, Ashkan Ehdaie, Russell Rogers, Daniel Soetkamp, Jackelyn Valle, Kevin Holm, Lizbeth Sanchez, Ileana Tremmel, Asma Nawaz, Michael Shehata, Xunzhang Wang, Adityo Prakosa, Joseph Yu, Jennifer E Van Eyk, Natalia Trayanova, Eduardo Marbán, and Eugenio Cingolani |
| Title | Plasma Torquetenovirus (TTV) microRNAs and severity of COVID-19 |
| Journal | Virology Journal volume. 2022. |
| Authors | Maria Alfreda Stincarelli, Andreina Baj, Bernardo Guidotti, Pietro Giorgio Spezia, Federica Novazzi, Ersilia Lucenteforte, Silvia Tillati, Daniele Focosi, Fabrizio Maggi & Simone Giannecchini |
| Title | Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection |
| Journal | BMC Genomics. 2022. |
| Authors | Xuehua Zhan, Wenqi Yuan, Yueyong Zhou, Rong Ma & Zhaohui Ge |
| Title | Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth |
| Journal | Host-Microbial Interactions. 2022. |
| Authors | Luke D. Halder, Svitlana Babych, Diana I. Palme, Elham Mansouri-Ghahnavieh, Lia Ivanov, Victory Ashonibare, Daniela Langenhorst |
| Title | Bioactivity and miRNome Profiling of Native Extracellular Vesicles in Human Induced Pluripotent Stem Cell-Cardiomyocyte Differentiation |
| Journal | Advanced Science. 2022. |
| Authors | Ana F. Louro,Marta A. Paiva,Marta R. Oliveira,Katharina A. Kasper,Paula M. Alves,Patrícia Gomes-Alves,Margarida Serra |
| Title | Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation |
| Journal | Front Physiol 2022. |
| Authors | Ana F. Louro, Nikolaus Virgolini, Marta A. Paiva, Inês A. Isidro, Paula M. Alves, Patrícia Gomes-Alves, and Margarida Serra |
| Title | P-196 Assessing human embryo implantation competence through analysis of extracellular vesicles and their contents |
| Journal | Human Reproduction 2022. |
| Authors | K.C Pavani, K Tilleman, D Stoop, B Gadella, A Hendrix, A Van Soom |
| Title | Transfer of microRNA-22-3p by M2 macrophage-derived extracellular vesicles facilitates the development of ankylosing spondylitis through the PER2-mediated Wnt/β-catenin axis |
| Journal | Cell Death Discovery 2022. |
| Authors | Liu C, Liang T, Zhang Z, Chen J, Xue J, Zhan X, Ren L |
| Title | Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection |
| Journal | BMC Genomics. May 2022. |
| Authors | Zhan, Xuehua, Wenqi Yuan, Yueyong Zhou, Rong Ma, and Zhaohui Ge. |
| Title | Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients |
| Journal | JCLA. 2021. |
| Authors | Miao Fu,Liping Fang,Xi Xiang,Xijing Fan,Junqi Wu,Jinhua Wang |
| Title | Molecular profiles and urinary biomarkers of upper tract urothelial carcinomas associated with aristolochic acid exposure |
| Journal | International Journal of Cancer 2021. |
| Authors | Sandra Karanović, Maude Ardin, Zuojian Tang, Karla Tomić, Stephanie Villar, Claire Renard, Elisa Venturini, Adam H. Lorch, Daniel S. Lee, Želimir Stipančić, Neda Slade, Ivana Vuković Brinar, Damir Dittrich, Krešimir Karlović, Fran Borovečki, Kathleen G. Dickman, Magali Olivier, Arthur P. Grollman, Bojan Jelaković, Jiri Zavadil |
| Title | Extracellular microRNA signature in chronic kidney disease |
| Journal | American Journal of Physiology. 2017. |
| Authors | Muralidharan J, Ramezani A, Hubal MJ, Knoblach S, Shrivastav S, Karandish S, Scott R, Maxwell N, Ozturk S, Beddhu S, Kopp JB, Raj DS. |
Exosomal RNA Isolation Kit
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits. This kit also allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
How to Isolate Exosomal RNA From Exosomes
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Sample type
|
Exosomes
purified using Norgen’s Purification Kits |
| Size of RNA Purified |
All sizes, including miRNA
and small RNA (< 200 nt) |
| Elution Volume |
50-100 μL
|
| Time to Complete 10 Purifications |
35-40 minutes
|
| Average Yields* |
Variable depending on specimen
|
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. 58000 (50 preps) |
|---|---|
| Lysis Buffer A | 20 mL |
| Lysis Additive B | 2 mL |
| Wash Solution A | 18 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Biological substrate modification suppresses ventricular arrhythmias in a porcine model of chronic ischaemic cardiomyopathy |
| Journal | Eur Heart J. 2022. |
| Authors | James F. Dawkins, Ashkan Ehdaie, Russell Rogers, Daniel Soetkamp, Jackelyn Valle, Kevin Holm, Lizbeth Sanchez, Ileana Tremmel, Asma Nawaz, Michael Shehata, Xunzhang Wang, Adityo Prakosa, Joseph Yu, Jennifer E Van Eyk, Natalia Trayanova, Eduardo Marbán, and Eugenio Cingolani |
| Title | Plasma Torquetenovirus (TTV) microRNAs and severity of COVID-19 |
| Journal | Virology Journal volume. 2022. |
| Authors | Maria Alfreda Stincarelli, Andreina Baj, Bernardo Guidotti, Pietro Giorgio Spezia, Federica Novazzi, Ersilia Lucenteforte, Silvia Tillati, Daniele Focosi, Fabrizio Maggi & Simone Giannecchini |
| Title | Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection |
| Journal | BMC Genomics. 2022. |
| Authors | Xuehua Zhan, Wenqi Yuan, Yueyong Zhou, Rong Ma & Zhaohui Ge |
| Title | Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth |
| Journal | Host-Microbial Interactions. 2022. |
| Authors | Luke D. Halder, Svitlana Babych, Diana I. Palme, Elham Mansouri-Ghahnavieh, Lia Ivanov, Victory Ashonibare, Daniela Langenhorst |
| Title | Bioactivity and miRNome Profiling of Native Extracellular Vesicles in Human Induced Pluripotent Stem Cell-Cardiomyocyte Differentiation |
| Journal | Advanced Science. 2022. |
| Authors | Ana F. Louro,Marta A. Paiva,Marta R. Oliveira,Katharina A. Kasper,Paula M. Alves,Patrícia Gomes-Alves,Margarida Serra |
| Title | Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation |
| Journal | Front Physiol 2022. |
| Authors | Ana F. Louro, Nikolaus Virgolini, Marta A. Paiva, Inês A. Isidro, Paula M. Alves, Patrícia Gomes-Alves, and Margarida Serra |
| Title | P-196 Assessing human embryo implantation competence through analysis of extracellular vesicles and their contents |
| Journal | Human Reproduction 2022. |
| Authors | K.C Pavani, K Tilleman, D Stoop, B Gadella, A Hendrix, A Van Soom |
| Title | Transfer of microRNA-22-3p by M2 macrophage-derived extracellular vesicles facilitates the development of ankylosing spondylitis through the PER2-mediated Wnt/β-catenin axis |
| Journal | Cell Death Discovery 2022. |
| Authors | Liu C, Liang T, Zhang Z, Chen J, Xue J, Zhan X, Ren L |
| Title | Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection |
| Journal | BMC Genomics. May 2022. |
| Authors | Zhan, Xuehua, Wenqi Yuan, Yueyong Zhou, Rong Ma, and Zhaohui Ge. |
| Title | Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients |
| Journal | JCLA. 2021. |
| Authors | Miao Fu,Liping Fang,Xi Xiang,Xijing Fan,Junqi Wu,Jinhua Wang |
| Title | Molecular profiles and urinary biomarkers of upper tract urothelial carcinomas associated with aristolochic acid exposure |
| Journal | International Journal of Cancer 2021. |
| Authors | Sandra Karanović, Maude Ardin, Zuojian Tang, Karla Tomić, Stephanie Villar, Claire Renard, Elisa Venturini, Adam H. Lorch, Daniel S. Lee, Želimir Stipančić, Neda Slade, Ivana Vuković Brinar, Damir Dittrich, Krešimir Karlović, Fran Borovečki, Kathleen G. Dickman, Magali Olivier, Arthur P. Grollman, Bojan Jelaković, Jiri Zavadil |
| Title | Extracellular microRNA signature in chronic kidney disease |
| Journal | American Journal of Physiology. 2017. |
| Authors | Muralidharan J, Ramezani A, Hubal MJ, Knoblach S, Shrivastav S, Karandish S, Scott R, Maxwell N, Ozturk S, Beddhu S, Kopp JB, Raj DS. |
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