Exosomal RNA Isolation Kit All-in-one system for the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits, ultracentrifugation, size-exclusion chromatography or most other commercial methods. For research use only and NOT intended for in vitro diagnostics.This kit is for use with Norgen's exosome purification technology only. View more Powered by Bioz Exosomal RNA Isolation Kit All-in-one system for the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits, ultracentrifugation, size-exclusion chromatography or most other commercial methods. Price USD$405.00 SKU 58000 Format Spin Column Size Variable Quantity Request sample Overview Details Documentation Citations Overview Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNABind and elute all RNA irrespective of size or GC content, without biasNo phenol extractionsNo Proteinase K treatmentNo carrier RNAConcentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µLPurify high-quality RNA in 15-20 minutesPurified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS servicesPurification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. This kit also allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Details Supporting Data Previous Figure 1. Isolation of RNA from exosomes purified from different urine volumes. Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different urine volumes purified using Norgen's Urine Exosome Purification Kits (Cat# 57700, 57800 and 57900). Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%. Figure 2. Isolation of RNA from exosomes purified from different plasma volumes. Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different plasma volumes purified using Norgen's Plasma/Serum Exosome Purification Kits (Cat# 57400, 57500 and 57600). Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Next Click for expanded view Kit SpecificationsSample typeExosomes purified using Norgen’s Purification Kits and most other methodsSize of RNA PurifiedAll sizes, including miRNAand small RNA (< 200 nt)Elution Volume50-100 μLTime to Complete 10 Purifications35-40 minutesAverage Yields*Variable depending on specimen*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.Storage Conditions and Product StabilityAll buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. ComponentCat. 58000 (50 preps)Lysis Buffer A20 mLLysis Additive B2 mLWash Solution A18 mLElution Solution A6 mLMini Spin Columns50Collection Tubes50Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS PI58000-5 Exosomal RNA Isolation Kit Insert.pdf SAFETY DATA SHEETS 58000_SDS.pdf FLYERS Exosome Purification Kit v3.0.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Services NGS RNA Isolation Related Products Urine Exosome Purification Mini Kit Plasma/Serum Exosome Purification Mini Kit
Exosomal RNA Isolation Kit All-in-one system for the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits, ultracentrifugation, size-exclusion chromatography or most other commercial methods. For research use only and NOT intended for in vitro diagnostics.This kit is for use with Norgen's exosome purification technology only. View more Powered by Bioz Exosomal RNA Isolation Kit All-in-one system for the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits, ultracentrifugation, size-exclusion chromatography or most other commercial methods. Price USD$405.00 SKU 58000 Format Spin Column Size Variable Quantity Request sample