Exosomal RNA Isolation Kit

Features and Benefits

Norgen’s Exosomal RNA Isolation Kit is an all-in-one system designed for the isolation of exosomal RNA from purified exosomes. This kit also enables the isolation of RNA from intact extracellular vesicles (EV) purified from urine, plasma/serum, cell culture media, and saliva, regardless of sample volume. The purification process utilizes spin column chromatography with Norgen’s proprietary resin. The kit is capable of isolating RNA of all sizes, including microRNA, and offers a significant advantage over other available kits by eliminating the need for special instruments, precipitation reagents, extension tubes, phenol/chloroform, or protease treatments. The purified RNA maintains the highest integrity and is suitable for various downstream applications.

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Supporting Data

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Figure 1. Isolation of RNA from exosomes purified from different urine volumes. Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different urine volumes purified using Norgen's Urine Exosome Purification Kits (Cat# 57700, 57800 and 57900). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The average Ct value for urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 2. Isolation of RNA from exosomes purified from different plasma volumes. Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different plasma volumes purified using Norgen's Plasma/Serum Exosome Purification Kits (Cat# 57400, 57500 and 57600). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The average Ct value for plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

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