Hop latent viroid (HLVd) TaqMan RT-PCR Detection Kits

Features and Benefits

RT-PCR-based detection kits for Hop Latent Viroid (HLVd)
Can be used with purified RNA from a variety of sample types
Available in real-time TaqMan format
Real-time format includes internal control and synthetic positive control to ensure your assay is working properly every step of the way
Pair with Norgen's Plant/Fungi RNA Purification Kit for the highest level of sensitivity in detection
Detect HLVd present in plant crops - Discover Now

Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA of 256 nucleotides. Initially it was believed that this viroid did not produce any symptoms of disease, thus the inclusion of the term latent in the viroid name. However it is now known that HLVd infection occurs worldwide in hop (Humulus lupulus) and is deleterious to production in certain cultivars. Quantitative changes in some secondary metabolites in the secretory glands forming lupulin are induced by HLVd infection, indicating that the viroid is not truly latent. Hop Latent Viroid infection depends on the metabolism of the host plant, and it’s replication and pathogenesis are mediated by its structural features and its ability to interact with cellular factors.

Click to expand options

HLVd TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

HLVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM38750 (100 preps)	Cat. TM38710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
HLVd Primer & Probe Mix	280 μL	280 μL
HLVd Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Hop latent viroid (HLVd) TaqMan RT-PCR Detection Kits