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HLVd Detection Kits (Cat. TM38700, TM38710, EP38700)

 

Purchasing Information

  • Detection kits for HLVd
  • Available in 2 formats for analysis:
TaqMan Probe
Cat. TM38700 End-Point PCR
Cat. EP38700
 

HLVd TaqMan RT-PCR Kit (Cat. TM38700) 24 rxns
HLVd TaqMan Probe/Primer and Control Set (Cat. TM38710) 100 rxns
HLVd End-Point RT-PCR Kit (Cat. EP38700) 24 rxns
HLVd Primer and Control Set (Cat. EP38710) 100 rxns

  • Detection kits for HLVd
  • Available in 2 formats for analysis:
TaqMan Probe
Cat. TM38700 End-Point PCR
Cat. EP38700
 


HLVd Detection Kits

Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA of 256 nucleotides. Initially it was believed that this viroid did not produce any symptoms of disease, thus the inclusion of the term latent in the viroid name. However it is now known that HLVd infection occurs worldwide in hop (Humulus lupulus) and is deleterious to production in certain cultivars. Quantitative changes in some secondary metabolites in the secretory glands forming lupulin are induced by HLVd infection, indicating that the viroid is not truly latent. Hop Latent Viroid infection depends on the metabolism of the host plant, and it’s replication and pathogenesis are mediated by its structural features and its ability to interact with cellular factors.

There are 2 kits available for the detection of HLVd:


Click to expand options
HLVd TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
HLVd TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
HLVd End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
HLVd End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


HLVd TaqMan RT-PCR Kit (Cat. TM38700) 24 rxns
HLVd TaqMan Probe/Primer and Control Set (Cat. TM38710) 100 rxns
HLVd End-Point RT-PCR Kit (Cat. EP38700) 24 rxns
HLVd Primer and Control Set (Cat. EP38710) 100 rxns

  • Detection kits for HLVd
  • Available in 2 formats for analysis:
TaqMan Probe
Cat. TM38700 End-Point PCR
Cat. EP38700
 


HLVd Detection Kits

Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA of 256 nucleotides. Initially it was believed that this viroid did not produce any symptoms of disease, thus the inclusion of the term latent in the viroid name. However it is now known that HLVd infection occurs worldwide in hop (Humulus lupulus) and is deleterious to production in certain cultivars. Quantitative changes in some secondary metabolites in the secretory glands forming lupulin are induced by HLVd infection, indicating that the viroid is not truly latent. Hop Latent Viroid infection depends on the metabolism of the host plant, and it’s replication and pathogenesis are mediated by its structural features and its ability to interact with cellular factors.

There are 2 kits available for the detection of HLVd:


Click to expand options
HLVd TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
HLVd TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
HLVd End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
HLVd End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

 

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