What is HLVd?
Hop Latent Viroid (HLVd) has become an ongoing concern for cultivators worldwide in the hemp and hops industries. It is a single-stranded, circular infectious RNA of 256 nucleotides.
- brittle stems
- yellow leaves
- reduced flower/trichome mass
- decreased yield
Typically in the early growing stages, infected plants will appear asymptomatic. By the time HLVd infection can be visually identified, it has often already spread to many other plants in the crop.
How Norgen Can Help Support You
- Open-platform compatibility: detection assays compatible with most commercially available real-time PCR systems with standard fluorescent channels (*see kit protocols for more details or contact us)
- Cost-effective solutions, whether you're a start-up laboratory or established testing provider
- Top-quality prodcts: Norgen Biotek has provided molecular testing solutions to the global community for over 20 years, with an ISO 9001 and ISO 13485 certified manufacturing facility
Whether you are running a commercial testing facility, are looking to start your own testing laboratory, or are performing plant pathogen research, Norgen offers a full workflow of products to take you from leaf punch to detection
HLVd Detection Process
Equipment You Need
- Benchtop Microcentrifuge
- 96-100% Ethanol
- Liquid Nitrogen + Mortar/Pestle OR another tissue homogenization tool
- Pipette Set and Tips
- PCR Tubes
- Vortex Mixer
- Real-Time PCR System
RNA EXTRACTION AND PURIFICATION
The sensitivity of any PCR assay relies heavily on the initial RNA or DNA extraction - ensuring you remove all contaminating components, while capturing even the smallest genetic fragments, is crucial. Ensure you're getting the most out of your plant sample with Norgen's Plant/Fungi Total RNA Purification Kit. Available in spin-column and 96-well plate formats.
- No hazardous phenol/chloroform
- Highest level of sensitivity
- Exceptionally robust for even the most challenging samples
- Available in column or 96-Well Plate format for high throughput
| Catalog Number | Product Name | Format | Prep Size | dryer |
|---|---|---|---|---|
| 25800 | Plant/Fungi Total RNA Purification Kit | column | 50 | |
| 31350 | Plant/Fungi Total RNA Purification Kit | column | 100 | |
| 25850 | Plant/Fungi Total RNA Purification Kit | column | 250 | |
| 31900 | Plant/Fungi Total RNA Purification 96-Well Kit | 2 x 96-well plates | 192 |
Our Plant/Fungi Total RNA Purification Kit can extract total RNA, including virus & viroid RNA. It offers a robust lysis buffer that is well-suited for challenging samples. Isolates total RNA (including microRNA) without phenol. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. Contact us for sample options.
REAL TIME PCR
PCR (RT-PCR) Real Time Polymerase Chain Reaction
Molecular detection is required to accurately detect and employ remediation strategies for challenging viral or viroid plant pathogens such as HLVd, with RT-PCR detection assays recognized as the gold standard detection method. Norgen offers a variety of TaqMan Real-Time PCR assays for Hop Latent Viroid (HLVd), Cannabis Cryptic Virus (CanCV), Lettuce Chlorosis Virus (LCV) and more.
Checkout a complete list of our PCR detection kits for plant pathogens.
![HLVd/LCV/CanCV TaqMan RT-PCR Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/images/product_images/TM69900-HLVd-LCV-CanCV-TaqMan-RT-PCR-KIT-WEB.jpg?itok=I7_rvYCv" "HLVd/LCV/CanCV TaqMan RT-PCR Kit")
| Catalog Number | Product Name | Number of Reactions | |
|---|---|---|---|
| TM38750 | HLVd TaqMan RT-PCR Kit | 100 | |
| TM38710 | HLVd TaqMan Probe/Primer and Control Set | 100 | |
| TM38950 | HSVd TaqMan RT-PCR Kit | 100 | |
| TM69950 | HLVd/LCV/CanCV TaqMan RT-PCR Kit | 100 |
Larger reaction sizes available. Contact us for details.
RESULTS
PCR gives results that:
- Provide a fast turnaround time to your customers
- Have the most accurate results for your plant samples
- Are reliable and leave you with consistent satisfaction
Contact the Experts
Get in touch with Norgen's on site product specialists for more information or a direct quote for the product you are interested in.
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