Viroid Detection

Stop the spread of devastating pathogens such as HLVd

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What is HLVd?

Hop Latent Viroid (HLVd) has become an ongoing concern for cultivators world wide in both Cannabis and Hemp industries. It is a single-stranded, circular infectious RNA of 256 nucleotides. Symptoms of this viral infection include; brittle stems, reduced flower / trichome mass, and decreased yield. Typically in the early growing stages these infected plants will often look asymptomatic and will only show visible symptoms in older plants. This is why sterile environments and early onset detection are vital for these plants.

How is HLVd Transmitted?

Transmission of HLVd is most commonly spread with non disinfected cultivativation instruments that have been infected from a previous plant. This is why in large greenhouses or cloning operations it leaves an immense opportunity for this viorid to spread. To help cultivators reduce this cycle, make sure to clean your equipment before moving to your next plant.

How Norgen Can Help Support You

The fast spread of this pathogen from plant to plant can be devastating without early detection methods. Therefore Molecular detection is required to accurately detect and employ remediation strategies for these challenging plant pathogens. RT-PCR is therefore the gold standard for early detection of HLVd to minimize spread to other plants. Norgen Biotek offers the most sensitive and streamlined procedure for purification of viroid RNA and detection of HLVd. Let us and our growing supporters help you improve your plant's quality and minimize losses at each stage of the growth process.

Talk to one of our molecular detection experts today!

A healthy plant (left) compared to a plant infected with Hop Latent Viroid (right).

Detection

Norgen Biotek HLVd Pathogen Detection

Norgen offers two ready to use kit formats for the detection of viroids and pathogens in plants.

TaqMan RT-PCR Kit

  • Involves the use of TaqMan primers and probes for use on real-time PCR detection systems

End-Point PCR Kit

  • For use on traditional thermocyclers with a subsequent gel electrophoresis analysis

We also offer PCR detection kits for:

Cat # Pathogen TaqMan End-Point
TM38900, EP38900 Hop stunt viroid
TM32900, EP32900 Aspergillus
TM41300, EP41300 E. Coli
TM33200, EP33200 Penicillium
TM32100, EP32100 Salmonella

Checkout a complete list of our PCR detection kits for plant pathogens.

Please note that our HLVd or any pathogen detection method does require a laboratory setting. NOT an in-field test.

Ordering Information

Cat. No Product Add to Cart
TM38700 HLVd TaqMan RT-PCR Kit
EP38700 HLVd End-Point RT-PCR Kit
TM69900 HLVd/LCV/CanCV TaqMan RT-PCR Kit

Contact the Experts

Get in touch with Norgen's on site product specialists for more information or a direct quote for the product you are interested in.

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Isolation and Purification

The sensitivity of any PCR assay relies heavily on the initial RNA or DNA extraction. Ensure you're getting the most out of your sample, and the most sensitive detection results, with our robust Plant/Fungi RNA and DNA purification kits. Norgen's purification kits will ensure you capture the full range of RNA or DNA present in your sample, setting you up for high-quality results downstream.

Plant RNA Isolation RNA Experts

Norgen's Purification Technology: Viroids are small, circular pieces of RNA that are responsible for a wide variety of diseases in plants. Typically, they are very small - ranging in size from only 246 to 463 nucleotides in length. Unfortunately, typical silica columns, magnetic beads and other traditional purification methods are unable to capture such short fragments efficiently. Norgen has overcome this problem by utilizing a patented silicon carbide a resin technology in our purification kits. This technology allows for capture of small, fragmented and degraded pieces of RNA that can be lost with traditional methods.

Learn more about Norgen's Silicon Carbide Technology!

Related Blogs

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Have you ever seen an apple with blotchy skin or an avocado with strange yellow markings? These abnormalities could be the result of the plant suffering from a viroid infection....

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In the past, diagnoses were limited to what could be observed by an attending physician with their own senses. As one might expect, this resulted in wildly varying levels of patient care and unconventional tests and treatments, many of which unfortunately involved livestock...

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Detect HLVd in Plant Crops, and protect your long term yield.