Viroid Detection

Stop the spread of devastating pathogens such as HLVd

What is HLVd?

Hop Latent Viroid (HLVd) has become an ongoing concern for cultivators worldwide in the hemp and hops industries. It is a single-stranded, circular infectious RNA of 256 nucleotides.

                        Symptoms of HLVd include:
                        brittle stems
yellow leaves
reduced flower/trichome mass
decreased yield

                    

Typically in the early growing stages, infected plants will appear asymptomatic. By the time HLVd infection can be visually identified, it has often already spread to many other plants in the crop.

How Norgen Can Help Support You

Open-platform compatibility: detection assays compatible with most commercially available real-time PCR systems with standard fluorescent channels (*see kit protocols for more details or contact us)
Cost-effective solutions, whether you're a start-up laboratory or established testing provider
Top-quality prodcts: Norgen Biotek has provided molecular testing solutions to the global community for over 20 years, with an ISO 9001 and ISO 13485 certified manufacturing facility

Whether you are running a commercial testing facility, are looking to start your own testing laboratory, or are performing plant pathogen research, Norgen offers a full workflow of products to take you from leaf punch to detection

Email one of our molecular detection experts today!

HLVd Detection Process

Equipment You Need

Benchtop Microcentrifuge
96-100% Ethanol
Liquid Nitrogen + Mortar/Pestle OR another tissue homogenization tool
Pipette Set and Tips
PCR Tubes
Vortex Mixer
Real-Time PCR System

RNA EXTRACTION AND PURIFICATION

STEP ONE

The sensitivity of any PCR assay relies heavily on the initial RNA or DNA extraction - ensuring you remove all contaminating components, while capturing even the smallest genetic fragments, is crucial. Ensure you're getting the most out of your plant sample with Norgen's Plant/Fungi Total RNA Purification Kit. Available in spin-column and 96-well plate formats.

                    Benefits of Norgen Extraction Kits:
                    No hazardous phenol/chloroform
Highest level of sensitivity
Exceptionally robust for even the most challenging samples
Available in column or 96-Well Plate format for high throughput

                

Catalog Number	Product Name	Format	Prep Size
25800	Plant/Fungi Total RNA Purification Kit	column	50
31350	Plant/Fungi Total RNA Purification Kit	column	100
25850	Plant/Fungi Total RNA Purification Kit	column	250
31900	Plant/Fungi Total RNA Purification 96-Well Kit	2 x 96-well plates	192

Our Plant/Fungi Total RNA Purification Kit can extract total RNA, including virus & viroid RNA. It offers a robust lysis buffer that is well-suited for challenging samples. Isolates total RNA (including microRNA) without phenol. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. Contact us for sample options.

REAL TIME PCR

STEP TWO

PCR (RT-PCR) Real Time Polymerase Chain Reaction

Molecular detection is required to accurately detect and employ remediation strategies for challenging viral or viroid plant pathogens such as HLVd, with RT-PCR detection assays recognized as the gold standard detection method. Norgen offers a variety of TaqMan Real-Time PCR assays for Hop Latent Viroid (HLVd), Cannabis Cryptic Virus (CanCV), Lettuce Chlorosis Virus (LCV) and more.

Checkout a complete list of our PCR detection kits for plant pathogens.

                        Now Offering a Real-Time PCR Multiplex Detection Kit For:
                        Hop Latent Viroid (HLVd)
Lettuce Chlorotic Virus (LCV)
Cannabis Cryptic Virus (CanCV)

                    

Catalog Number	Product Name	Number of Reactions
TM38750	HLVd TaqMan RT-PCR Kit	100
TM38710	HLVd TaqMan Probe/Primer and Control Set	100
TM38950	HSVd TaqMan RT-PCR Kit	100
TM69950	HLVd/LCV/CanCV TaqMan RT-PCR Kit	100

Larger reaction sizes available. Contact us for details.

RESULTS

STEP THREE

                    PCR gives results that:
                    Provide a fast turnaround time to your customers
Have the most accurate results for your plant samples
Are reliable and leave you with consistent satisfaction

                

Contact the Experts

Get in touch with Norgen's on site product specialists for more information or a direct quote for the product you are interested in.

Get Quote

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Detect HLVd in Plant Crops, and protect your long term yield.