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Unlike the visual symptoms of fungal and bacterial pathogens, plant viroids and viruses are often asymptomatic. Molecular detection is therefore required to accurately detect and employ remediation strategies for these challenging plant pathogens.
There are many pathogens that have been identified to affect crop production. Hop Latent Viroid (HLVd) has recently become an increasing concern for growers. Infected plants and clones are often asymptomatic until later in their growth cycle - allowing plenty of time for the virus to spread throughout the crop. Symptoms of this viral infection include stunted growth, reduced yields and reduced potency.
RT-PCR is therefore the gold standard for early detection of HLVd to minimize spread to other plants. Norgen Biotek offers the most sensitive and streamlined procedure for purification of viroid RNA and detection of HLVd. Let us help you improve plant quality and minimize losses at each stage of the growth process.
A healthy plant (left) compared to a plant infected with Hop Latent Viroid (right).
Norgen offers two ready to use kit formats for the detection of viroids and pathogens in plants.
TaqMan RT-PCR Kit
End-point PCR Kit
We also offer PCR detection kits for:
| Cat. No | Product | Add to Cart |
|---|---|---|
| TM38700 | HLVd TaqMan RT-PCR Kit | |
| EP38700 | HLVd End-Point RT-PCR Kit |
Get in touch with Norgen’s on site product specialists for more information or a direct quote for the product you are interested in.
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Norgen also offers full service purification and detection services. Please contact us to learn more about our free consultation services.
The sensitivity of any PCR assay relies heavily on the initial RNA or DNA extraction. Ensure you’re getting the most out of your sample, and the most sensitive detection results, with our robust Plant/Fungi RNA and DNA purification kits. Norgen’s purification kits will ensure you capture the full range of RNA or DNA present in your sample, setting you up for high-quality results downstream.
Norgen’s Purification Technology: Viroids are small, circular pieces of RNA that are responsible for a wide variety of diseases in plants. Typically, they are very small - ranging in size from only 246 to 463 nucleotides in length. Unfortunately, typical silica columns, magnetic beads and other traditional purification methods are unable to capture such short fragments efficiently. Norgen has overcome this problem by utilizing a patented silicon carbide resin technology in our purification kits. This technology allows for capture of small, fragmented and degraded pieces of RNA that can be lost with traditional methods.
Available in spin-column and high-throughput 96-well plate formats.
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