Viroid Detection

Viroid Detection

Stop the spread of devastating pathogens such as HLVd

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What is HLVd?

Hop Latent Viroid (HLVd) has become an ongoing concern for cultivators worldwide in the hemp and hops industries. It is a single-stranded, circular infectious RNA of 256 nucleotides.

Symptoms of HLVd include:
  • brittle stems
  • yellow leaves
  • reduced flower/trichome mass
  • decreased yield
healthy vs infected cannabis plant

Typically in the early growing stages, infected plants will appear asymptomatic. By the time HLVd infection can be visually identified, it has often already spread to many other plants in the crop.

Read more about the detection process in our exclusive infographic, "HLVd: How to Identify and Detect this Devestating Pathogen"

How Norgen Can Help Support You

  • Open-platform compatibility: detection assays compatible with most commercially available real-time PCR systems with standard fluorescent channels (*see kit protocols for more details or contact us)
  • Cost-effective solutions, whether you're a start-up laboratory or established testing provider
  • Top-quality prodcts: Norgen Biotek has provided molecular testing solutions to the global community for over 20 years, with an ISO 9001 and ISO 13485 certified manufacturing facility

Whether you are running a commercial testing facility, are looking to start your own testing laboratory, or are performing plant pathogen research, Norgen offers a full workflow of products to take you from leaf punch to detection

Email one of our molecular detection experts today!

HLVd Detection Process

Equipment You Need

tools needed for HLVd testing
  • Benchtop Microcentrifuge
  • 96-100% Ethanol
  • Liquid Nitrogen + Mortar/Pestle OR another tissue homogenization tool
  • Pipette Set and Tips
  • PCR Tubes
  • Vortex Mixer
  • Real-Time PCR System

RNA EXTRACTION AND PURIFICATION

1
STEP ONE
rna extraction and purification step

The sensitivity of any PCR assay relies heavily on the initial RNA or DNA extraction - ensuring you remove all contaminating components, while capturing even the smallest genetic fragments, is crucial. Ensure you're getting the most out of your plant sample with Norgen's Plant/Fungi Total RNA Purification Kit. Available in spin-column and 96-well plate formats.

Benefits of Norgen Extraction Kits:
  • No hazardous phenol/chloroform
  • Highest level of sensitivity
  • Exceptionally robust for even the most challenging samples
  • Available in column or 96-Well Plate format for high throughput
Plant RNA Isolation RNA Experts
Catalog Number Product Name Format Prep Size
25800 Plant/Fungi Total RNA Purification Kit column 50
31350 Plant/Fungi Total RNA Purification Kit column 100
25850 Plant/Fungi Total RNA Purification Kit column 250
31900 Plant/Fungi Total RNA Purification 96-Well Kit 2 x 96-well plates 192

Our Plant/Fungi Total RNA Purification Kit can extract total RNA, including virus & viroid RNA. It offers a robust lysis buffer that is well-suited for challenging samples. Isolates total RNA (including microRNA) without phenol. Purification is based on spin column chromatography that uses Norgen's proprietary Silicon Carbide resin separation matrix. Contact us for sample options.

Learn more about Norgen's Silicon Carbide Technology!

REAL TIME PCR

2
STEP TWO
PCR machine diagram

PCR (RT-PCR) Real Time Polymerase Chain Reaction

Molecular detection is required to accurately detect and employ remediation strategies for challenging viral or viroid plant pathogens such as HLVd, with RT-PCR detection assays recognized as the gold standard detection method. Norgen offers a variety of TaqMan Real-Time PCR assays for Hop Latent Viroid (HLVd), Cannabis Cryptic Virus (CanCV), Lettuce Chlorosis Virus (LCV) and more.

Checkout a complete list of our PCR detection kits for plant pathogens.

Now Offering a Real-Time PCR Multiplex Detection Kit For:

  • Hop Latent Viroid (HLVd)
  • Lettuce Chlorotic Virus (LCV)
  • Cannabis Cryptic Virus (CanCV)
HLVd/LCV/CanCV TaqMan RT-PCR Kit
Catalog Number Product Name Number of Reactions
TM38750 HLVd TaqMan RT-PCR Kit 100
TM38710 HLVd TaqMan Probe/Primer and Control Set 100
TM38950 HSVd TaqMan RT-PCR Kit 100
TM69950 HLVd/LCV/CanCV TaqMan RT-PCR Kit 100

Larger reaction sizes available. Contact us for details.

RESULTS

3
STEP THREE
sending results to HLVd report

PCR gives results that:

  • Provide a fast turnaround time to your customers
  • Have the most accurate results for your plant samples
  • Are reliable and leave you with consistent satisfaction

Contact the Experts

Get in touch with Norgen's on site product specialists for more information or a direct quote for the product you are interested in.

Get Quote

Lea Product Sales Specialist

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Detect HLVd in Plant Crops, and protect your long term yield.