Plasma/Serum Exosome Purification Kit
Quick and easy protocol for the purification of exosomes from different plasma/serum sample volumes
For research use only and NOT intended for in vitro diagnostics.
Features and Benefits
- Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
- Versatile sample input ranging from 50 µL to 10 mL
- Plasma/Serum Exosome Purification Mini Kit (50 µL - 1 mL Plasma/Serum)
- Plasma/Serum Exosome Purification Midi Kit (1 mL - 4 mL Plasma/Serum)
- Plasma/Serum Exosome Purification Maxi Kit (4 mL - 10 mL Plasma/Serum)
- Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
- No precipitation reagents, overnight incubation, protease or coagulant treatments required
- No time-consuming ultracentrifugation, filtration or special syringes required
- Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
- Purified exosomes are compatible with functional studies.
- Purified exosomes are free from any RNA-binding proteins
- Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
- Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
To purify exosomes and isolate exosomal RNA, choose the Plasma/serum Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA/TEM etc. before processing them for RNA isolation. Or you can use the Exosomal RNA Isolation Kit if you’ve already purified exosomes using a Norgen kit or another method. . Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.
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