Plasma/Serum Exosome Purification Kit
Quick and easy protocol for the purification of exosomes from different plasma/serum sample volumes
For research use only and NOT intended for in vitro diagnostics.
Plasma/Serum Exosome Purification Kit
Quick and easy protocol for the purification of exosomes from different plasma/serum sample volumes
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Features and Benefits
- Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
- Versatile sample input ranging from 50 µL to 10 mL
- Plasma/Serum Exosome Purification Mini Kit (50 µL - 1 mL Plasma/Serum)
- Plasma/Serum Exosome Purification Midi Kit (1 mL - 4 mL Plasma/Serum)
- Plasma/Serum Exosome Purification Maxi Kit (4 mL - 10 mL Plasma/Serum)
- Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
- No precipitation reagents, overnight incubation, protease or coagulant treatments required
- No time-consuming ultracentrifugation, filtration or special syringes required
- Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
- Purified exosomes are compatible with functional studies.
- Purified exosomes are free from any RNA-binding proteins
- Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
- Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
To purify exosomes and isolate exosomal RNA, choose the Plasma/serum Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA/TEM etc. before processing them for RNA isolation. Or you can use the Exosomal RNA Isolation Kit if you’ve already purified exosomes using a Norgen kit or another method. . Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.
Details
Supporting Data
Kit Specifications
|
|
Plasma/Serum Input (Cat. 57400) |
50 μL - 1 mL
|
Plasma/Serum Input (Cat. 57500)
|
1 mL - 4 mL
|
Plasma/Serum Input (Cat. 57600) | 4 mL - 10 mL |
Size of Exosomes Purified
|
40 nm - 150 nm
|
Elution Volume | Variable depending on the plasma/serum input volume |
Time to Complete 10 Purifications |
15 - 30 minutes
|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
Component | Cat. 57400 (50 preps) | Cat. 57500 (25 preps) | Cat. 57600 (15 preps) |
---|---|---|---|
Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
ExoC Buffer | 8 mL | 8 mL | 2 x 8 mL |
ExoR Buffer | 12 mL | 12 mL | 12 mL |
Mini Filter Spin Columns inserted into 2 mL tubes | 50 | 25 | 15 |
Product Insert | 1 | 1 | 1 |
Documentation
FAQs
Mini, Midi, Maxi
Citations
Title | Central nervous system-derived extracellular vesicles: the next generation of neural circulating biomarkers? |
Citation | Translational Neurodegeneration 2023. |
Authors | Rocío del Carmen Bravo-Miana,corresponding author1,2 Jone Karmele Arizaga-Echebarria,1,2 and David Otaegui |
Title | Characterization of PSA associated with EVs from prostate cancer samples to develop a lateral flow diagnostic test. |
Citation | intuitive Dx 2023. |
Authors | Scott-Weathers, C., King, K., and Luke, K |
Title | High throughput Surface Epitope Immunoaffinity Isolation of Extracellular Vesicles and Downstream Analysis. |
Citation | Biology Methods and Protocols 2023. |
Authors | Ramin Khanabdali1*#, Michelle Mandrekar2 , Rick Grygiel2 , Phuoc-an Vo2 , Carlos Palma1 , Sara Nikseresht1 , Siena Barton1 , Mozhgan Shojaee1 , Sadman Bhuiyan1 , Kartini Asari1 , Susan Belzer1 , Khairul Ansari1 , Jermaine I Coward 3,4, Lewis Perrin 3, John Hooper3 , Dominic Guanzon5 , Andrew Lai4 , Carlos Salomon5 , Kevin Kershner2 , Christine Newton2 , Douglas Horejsh2 , Gregory Rice 1 5 |
Title | Potential cardiac-derived exosomal miRNAs involved in cardiac healing and remodeling after myocardial ischemia-reperfusion injury |
Citation | Scientific Reports 2023. |
Authors | Yu Liu, Jiao Chen, Jian Xiong, Jin-Qun Hu, Li-Yuan Yang, Yu-Xin Sun, Ying Wei, Yi Zhao, Xiao Li, Qian-Hua Zheng, Wen-Chuan Qi & Fan-Rong Liang |
Title | Proteomic analysis of human serum Extracellular Vesicles reveals early diagnostic markers for Amyotrophic Lateral Sclerosis |
Citation | medrxiv 2023. |
Authors | Natasha Vassileff, Pascal Leblanc, Emilien Bernard, Anthony Fourier, Isabelle Quadrio, Rohan G. T. Lowe, Jereme G. Spiers, Andrew F. Hill, Lesley Cheng |
Title | X-ray Photoelectron Spectroscopy (XPS) Analysis of Nitrogen Environment in Small Extracellular Vesicle Membranes: A Potential Novel Technique with Application for Cancer Screening |
Citation | Cancers 2023. |
Authors | María Sancho-Albero, Ana Martín-Pardillos, Silvia Irusta, Víctor Sebastián, Vicente Luis Cebolla, Roberto Pazo-Cid , Pilar Martín-Duque and Jesús Santamaría |