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| Items | Cat. # | Size | |
|---|---|---|---|
| Plasma/Serum Exosome Purification Mini Kit | 57400 | 50 preps | |
| Plasma/Serum Exosome Purification Midi Kit | 57500 | 25 preps | |
| Plasma/Serum Exosome Purification Maxi Kit | 57600 | 15 preps |
Plasma/Serum Exosome Purification Kit
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Plasma/Serum Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA.
|
Kit Specifications
|
|
| Plasma/Serum Input (Cat. 57400) |
50 μL - 1 mL
|
|
Plasma/Serum Input (Cat. 57500)
|
1 mL - 4 mL
|
| Plasma/Serum Input (Cat. 57600) | 4 mL - 10 mL |
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Elution Volume | Variable depending on the plasma/serum input volume |
| Time to Complete 10 Purifications |
15 - 30 minutes
|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
| Items | Cat. # | Size | |
|---|---|---|---|
| Plasma/Serum Exosome Purification Mini Kit | 57400 | 50 preps | |
| Plasma/Serum Exosome Purification Midi Kit | 57500 | 25 preps | |
| Plasma/Serum Exosome Purification Maxi Kit | 57600 | 15 preps |
Plasma/Serum Exosome Purification Kit
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Plasma/Serum Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA.
|
Kit Specifications
|
|
| Plasma/Serum Input (Cat. 57400) |
50 μL - 1 mL
|
|
Plasma/Serum Input (Cat. 57500)
|
1 mL - 4 mL
|
| Plasma/Serum Input (Cat. 57600) | 4 mL - 10 mL |
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Elution Volume | Variable depending on the plasma/serum input volume |
| Time to Complete 10 Purifications |
15 - 30 minutes
|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
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