Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 50 µL to 10 mL
Plasma/Serum Exosome Purification Mini Kit (50 µL - 1 mL Plasma/Serum)
Plasma/Serum Exosome Purification Midi Kit (1 mL - 4 mL Plasma/Serum)
Plasma/Serum Exosome Purification Maxi Kit (4 mL - 10 mL Plasma/Serum)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
Exosmal RNA Analysis
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Plasma/Serum Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA.
Figure 1. Intact exosomes were purified from 1 mL plasma using different purification methods. Intact exosomes were purified from 1 mL plasma using Norgen's Plasma/Serum Exosome Purification Mini Kit (Cat# 57400), Competitor S's kit and Competitor L's kit (from plasma). Exosomes purified using Norgen's kit were resuspended in 200 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit isolated 55 nm exosomes with a recovery of 9.64 x 1013 particles/mL plasma. No impurities were found to be contaminating the exosomes purified using Norgen's Plasma/Serum Exosome Purification Mini Kit. Additionally, exosomes with a broader size range covering from 50 nm - 150 nm were purified from 1 mL plasma with a higher concentration compared to the other two methods.
Figure 2. Intact exosomes purified from 1 mL and 10 mL plasma. Intact exosomes were purified from 1 mL plasma using Norgen's Plasma/Serum Exosome Purification Mini Kit (Cat. 57400) and from 10 mL plasma using Norgen's Plasma/Serum Exosome Purification Maxi Kit (Cat. 57600). Exosomes purified using Norgen's Mini kit were resuspended in 200 µL of Norgen's ExoR buffer whereas exosomes purified using Norgen's Maxi kit were resuspended in 600 µL Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that the purification of exosomes is linear as 4.04 x 1010 particles/mL was recovered from 1 mL plasma whereas 2.95 x 1011 particles/mL was recovered from 10 mL plasma.
Figure 3. TEM images of small EVs obtained from the same non disease frozen platelet free plasma (ND-frozen PFP) sample. Sample isolated with three commercial kits; representative of >10 images (scale bar, 200 nm).
Figure 4. Western blot analysis of plasma small EVs (psEV). Plasma small EVs (psEV) isolated with the Norgen Biotek kit (#57400) using EV markers (TSG101, CD81, ALIX, CD63) and platelet markers (ITGA2B and SELP). Lysates of psEV derived from frozen Non-disease platelet free plasma (ND-PFP) are compared