Plasma/Serum RNA Purification Kits

Cat. 55000, 56100, 56200

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Plasma/Serum RNA Purification Kits

Cat. 55000, 56100, 56200

For rapid and simple purification of circulating RNA and Exosomal RNA from plasma/serum samples

Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma/serum input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into a flexible elution volume
High quality, purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Compatible with Streck Cell-Free RNA BCT® Tubes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Plasma/Serum RNA Purification Kit
(Cat. 55000) 50 preps

Plasma/Serum RNA Purification Midi Kit
(Cat. 56100) 20 preps

Plasma/Serum RNA Purification Maxi Kit
(Cat. 56200) 10 preps

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

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Kit Specifications

You have selected: Cat. 55000

Kit Specifications
Sample Volume Range	50 to 200 μL
Anti-coagulant (for Plasma)*	EDTA or Citrate
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Minimum Elution Volume	10 μL
Maximum Elution Volume	25 μL
Time to Complete 10 Purifications	15-20 minutes
Average Yield**	Variable depending on specimen

*This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

**Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 56100

Kit Specifications
Minimum Plasma/Serum Input	250 μL
Maximum Plasma/Serum Input	1.5 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields*	Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 56200

Kit Specifications
Minimum Plasma/Serum Input	2 mL
Maximum Plasma/Serum Input	5 mL
Size of DNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields*	Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

Supporting Data

Figure 1. Purification of circulating RNA from different plasma volumes. Norgen's Plasma/Serum RNA Purification Mini Kit was used to purify circulating RNA from 50 µL, 100 µL and 200 µL plasma prepared from blood collected on EDTA. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect miR-21 (Figure 1A) and the housekeeping 5S rRNA transcript (Figure 1B). The relative amount of both the miR-21 (Figure 1A) and the 5S rRNA transcript (Figure 1B) is linearly increasing with increasing the sample input volume.

Figure 2. Eluting purified circulating RNA into different elution volumes. Norgens Plasma/Serum RNA Purification Mini Kit was used to purify circulating RNA from 200 µL plasma prepared from blood collected on EDTA and eluted in 10 µL, 15 µL, and 25 µL. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect miR-21 (Figure 2A) and the housekeeping 5S rRNA transcript (Figure 2B). The relative amount of both the miR-21 (Figure 2A) and the 5S rRNA transcript (Figure 2B) is increasing with increasing the elution volume indicating the efficient concentration of the plasma circulating RNA in a very low elution volume.

Figure 3. Effective and consistent detection of miRNA from plasma. Norgen's Plasma/Serum RNA Purification Mini Kit can effectively isolate miRNA from plasma. Circulating miRNA was isolated from 200 µL plasma using Norgen's Plasma/Serum RNA Purification Mini Kit, competitor Q's kit and competitor E's kit. Circulating miRNA was isolated from 600 µL using competitor A's kit. Stem loop RT-qPCR using primers specific to miR-21 was performed. In brief, 1 microliter of the 15 µL RNA purified using Norgen's Plasma/Serum RNA Purification Mini Kit, competitor Q's kit and 3.3 microliters of the 50 µL purified RNA using competitor E's kit and competitor A's kit was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21. Norgen's Plasma/Serum RNA Purification Mini Kit showed the most consistent and the highest recovery of the miR-21 transcripts as compared to the other isolation methods. The recovery of the miRNA from 200 µL plasma using Norgen's kit was higher than that recovered from RNA purified from 600 µL using competitor A's kit.

Figure 4. Small RNA Sequencing from as little as 50 µL of Plasma.Norgen Biotek has developed an effective pipeline for small RNA sequencing from small volumes of plasma/serum. RNA can be effectively and consistently recovered from as little as 50 µL of plasma using Norgen's patented sample preparation technology (example shown here withPlasma/Serum RNA Purification Mini Kit, Cat. 55000). Panel A shows that the number of microRNA detected from 50 or 200 µL of plasma was almost identical to that of 4 mL. Panel B is a Venn diagram showing that of the microRNAs identified, the majority are detected in all volumes of plasma input from 50 µL to 4 mL. In fact, the scatter plots in Panel C show that the relative expression level of each microRNA detected was highly correlated between 50 or 200 µL of plasma and 4 mL plasma.

Figure 5. High Degree of Overlapping of miRNA profile between Urine and Plasma. Plasma and mid-stream urine was collected from three different healthy individuals. RNA was isolated from 20 mL of each urine sample using Norgen's Urine Exosomal RNA Purification Kit (Cat. 47200) and 200 µL of each plasma sample using Norgen's Plasma/Serum RNA Purification Mini Kit (Cat. 55000). Small RNA Libraries were then generated using an Illumina TruSeq Small RNA Library Preparation Kit and subsequently sequenced on an Illumina MiSeq system. The list of mapped miRNAs from plasma and urine from the same individual was then compared. The Venn diagrams showed that the miRNA profiles have high degree of overlapping between urine and plasma within each individual.

Figure 6. Increased Diversity of Other Small RNA Species (Piwi-Interacting) in Urine. Plasma and mid-stream urine was collected from three different healthy individuals. RNA was isolated from 20 mL of each urine sample using Norgen's Urine Exosomal RNA Purification Kit (Cat. 47200) and 200 µL of each plasma sample using Norgen's Plasma/Serum RNA Purification Mini Kit (Cat. 55000). Small RNA Libraries were then generated using an Illumina TruSeq Small RNA Library Preparation Kit and subsequently sequenced on an Illumina MiSeq system. The above chart showed that the relative proportion of piwi-interacting RNA (piRNA) was consistently higher in urine.

Figure 7. Better Diversity of miRNA Detected from HeLa Cells using Illumina Small RNA Next Gen Sequencing.Norgen's Total RNA Purification Kit isolates miRNA from HeLa cells with better diversity than a leading competitor. Total RNA including miRNA was isolated from 1 million HeLa cells using Norgen's Total RNA Purification Kit and Competitor Q's leading miRNA Kit, and was applied to Illumina Small RNA Next Gen Sequencing on a MiSeq sequencer. Panel A showed that Norgens Total RNA Purification Kit recovers ahigher number of miRNAsthan the competitor. In particular, the higher diversity is achieved with a faster and simpler procedure in as little as 20 minutes of RNA sample preparation time without the use of phenol. Panel B is a scatter plot of the average RPM (reads per million) of the miRNAs detectedto compare Norgen and the competitorsrecovery of miRNA. Norgens Total RNA Purification Kit recovered a significantly higher number of miRNAs that have higher RPM, as summarized in the graph insert as well as in Panel C.

Figure 8. Purification of cell-free circulating RNA and exosomal RNA from different plasma volumes. Norgens Plasma/Serum RNA Purification Midi Kit (Cat# 56100) was used to purify cell-free circulating and exosomal RNA from 0.25 mL, 0.75 mL and 1.5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of the (A) housekeeping 5S rRNA transcript and (B) miR-21. The average Ct value for both the 5S rRNA transcript and the miR-21 is linearly decreasing with increasing the sample input volume.

Figure 9. Linearity of RNA purified from increasing plasma volumes using Norgens Plasma/Serum RNA Purification Midi Kit. Norgens Plasma/Serum RNA Purification Midi Kit (Cat# 56100) was used to purify RNA from 0.25 mL, 0.7 mL and 1.5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of (A) the housekeeping 5S rRNA transcript and (B) miR-21 from the different plasma volumes. Norgens Plasma/Serum RNA Purification Midi Kit was able to recover 97% of both the 5S rRNA transcript and the miR-21 transcript from 0.75 mL plasma relative to the amount that is present in 0.35 mL plasma. Moreover, 95% of the 5S rRNA transcript and the miR-21 was recovered from 1.5 mL plasma relative to the amount that is present in 0.75 mL plasma.

Figure 10. Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and miR-21. RNA was isolated from 0.25 mL, 0.75 mL and 1.5 mL plasma using Norgens Plasma/Serum RNA Purification Midi Kit (Cat# 56100). Increasing volumes of the elution (2, 4 and 8 µL) were used in a 20 µL reverse transcription reaction followed by qPCR amplification reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in the PCR input volume used as a template in the reverse transcription reaction did not affect the Ct value generated from the qPCR amplification for both (A) 5S rRNA transcript and (B) miR-21. In fact the Ct values tend to decrease with increasing the PCR input volume indicating that RNA purified from plasma using Norgens kit is free of the common inhibitors usually present in plasma.

Figure 10. Purification of cell-free circulating RNA and exosomal RNA from different plasma volumes. Norgens Plasma/Serum RNA Purification Maxi Kit (Cat# 56200) was used to purify cell-free circulating and exosomal RNA from 1.5 mL, 3 mL and 5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two millilitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of (A) the housekeeping 5S rRNA transcript and (B) miR-21. The average Ct value for both the 5S rRNA transcript and the miR-21 is linearly decreasing with increasing the sample input volume.

Figure 11. Linearity of RNA purified from increasing plasma volumes using Norgens Plasma/Serum RNA Purification Maxi Kit. Norgens Plasma/Serum Cell-Free Circulating and Exosomal RNA Purification Maxi Kit (Cat# 56200) was used to purify RNA from 1.5 mL, 3 mL and 5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of (A) the housekeeping 5S rRNA transcript and (B) miR-21 from the different plasma volumes. Norgens Plasma/Serum RNA Purification Maxi Kit was able to recover 92% of the 5S rRNA transcript from 3 mL plasma relative to the amount that is present in 1.5 mL plasma. Moreover, 90% of the 5S rRNA transcript was recovered from 5 mL plasma relative to the amount that is present in 3 mL plasma. As for miR-21, Norgens Plasma/Serum RNA Purification Maxi Kit was able to recover 91% of miR-21 from 3 mL plasma relative to the amount that is present in 1.5 mL plasma. Furthermore, 90% of miR-21 was recovered from 5 mL plasma relative to the amount that is present in 3 mL plasma.

Figure 12. Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and miR-21. DNA was isolated from 1.5 mL, 3 mL and 5 mL plasma using Norgens Plasma/Serum RNA Purification Maxi Kit (Cat# 56200). Increasing volumes of the elution (2, 4 and 8 µL) were used in a 20 µL reverse transcription reaction followed by qPCR amplification reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in the PCR input volume used as a template in the reverse transcription reaction did not affect the Ct value generated from the qPCR amplification for both (A) 5S rRNA transcript and (B) miR-21. In fact the Ct. values tend to decrease with increasing the PCR input volume indicating that RNA purified from plasma using Norgens kit is free of the common inhibitors usually present in plasma.

Click for expanded view

Protocols:

(55000, 56100, 56200) Plasma/Serum RNA Purification Kits - Protocol (50 Preps, 20 Preps, 10 Preps)

Supplementary Protocols:

Plasma/Serum RNA Purification Mini Kit - Supplementary Protocol for Exosomal RNA Purification from Exosomes Already Purified

Plasma/Serum RNA Purification Mini Kit - Supplementary Protocol for miRNA Isolation from ExoCompleteTM 96-Well Plate Kit

Application Notes:

Plasma Exosomal microRNA Purification from Hitachi’s ExoComplete™ 96-Well Plate Kit

Safety Data Sheets:

(55000) Plasma/Serum RNA Purification Mini Kit - Safety Data Sheet

(56100, 56200) Plasma/Serum RNA Purification Midi/Maxi Kit - Safety Data Sheet

Product Flyers:

Plasma/Serum RNA Purification Kits

Mini (55000)

Title	Evidence for Effects of Extracellular Vesicles on Physical, Inflammatory, Transcriptome and Reward Behaviour Status in Mice
Journal	Int. J. Mol. Sci. 2022
Authors	Nagiua Cuomo-Haymour, Hannes Sigrist, Christian Ineichen, Giancarlo Russo, Ursina Nüesch, Felix Gantenbein, Luka Kulic, Irene Knuesel, Giorgio Bergamini and Christopher Robert Pryce

Title	Identification and characterization of extracellular vesicles from red cells infected with Babesia divergens and Babesia microti
Journal	Front. Cell. Infect. Microbiol. 2022
Authors	Divya Beri, Marilis Rodriguez, Manpreet Singh, Yunfeng Liu, Giselle Rasquinha, Xiuli An, Karina Yazdanbakhsh and Cheryl A. Lobo

Title	Clinicopathological utility of miR-203a-3p in diagnosing colorectal cancer
Journal	Mol Biol Rep. 2022
Authors	Mahdi Qasemi Rad, Vahid Pouresmaeil, Fatemeh Hosseini Mojahed, Amir Amirabadi, Amir Hossein Aalami

Title	Lower miR-21/ROS/HNE levels associate with lower glycemia after habit-intervention: DIAPASON study 1-year later
Journal	Cardiovascular Diabetology. 2022
Authors	Lucia La Sala, Elena Tagliabue, Simona Mrakic-Sposta, Anna Chiara Uccellatore, Pamela Senesi, Ileana Terruzzi, Emilio Trabucchi, Luigi Rossi-Bernardi & Livio Luzi

Title	The clinical utility of cfRNA for disease detection and surveillance: A proof of concept study in non-small cell lung cancer
Journal	Thoracic Cancer. 2022
Authors	Martin Metzenmacher, Balazs Hegedüs, Jan Forster,Alexander Schramm, Peter A. Horn, Christoph A. Klein, Nicola Bielefeld, Till Ploenes, Clemens Aigner, Jens T. Siveke, Martin Schuler, Smiths S. Lueong

Title	Differential Expression of Serum Extracellular Vesicle miRNAs in Multiple Sclerosis: Disease-Stage Specificity and Relevance to Pathophysiology
Journal	Int. J. Mol. Sci. 2022
Authors	Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce

Title	Differential expression of serum extracellular vesicle microRNAs and analysis of target-gene pathways in major depressive disorder
Journal	Biomarkers in Neuropsychiatry. 2022
Authors	Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce

Title	Predictive capacity of a miRNA panel in identifying teratoma in post-chemotherapy consolidation surgeries
Journal	BJUI International. 2022
Authors	Joseph A. Moore,Michael J. Lehner,Simone Anfossi,Saumil Datar,Rebecca S. Tidwell,Matthew Campbell,Amishi Y. Shah,John F. Ward,Jose A. Karam,Christopher G. Wood,Lois L. Pisters,George A. Calin,Shi-Ming Tu

Title	Downregulation of Talin‐1 is associated with the increased expression of miR‐182‐5p and miR‐9‐5p in coronary artery disease
Journal	J Clin Lab Anal. 2022
Authors	Akram Gholipour, Farshad Shakerian, Ali Zahedmehr, Shiva Irani, Seyed Javad Mowla, and Mahshid Malakootian

Title	miR3-22-p as a Novel Biomarker in Rheumatoid Arthritis
Journal	Medicine Personalized Journal. 2022
Authors	Maryam Hassanpoor, Bahareh Abbasi

Title	Increased expression of miR-224-5p in circulating extracellular vesicles of patients with reduced coronary flow reserve
Journal	BMC Cardiovascular Disorders. 2022
Authors	Kreema James, Paulina Bryl-Gorecka, Björn Olde, Olof Gidlof, Kristina Torngren & David Erlinge

Title	Variability in Cerebrospinal Fluid MicroRNAs Through Life
Journal	Molecular Neurobiology. 2020
Authors	Prieto-Fernández, Endika, et al.

Title	Serum microRNA-34a is potential biomarker for inflammation in nonalcoholic fatty liver disease
Journal	Asian Biomedicine. 2016.
Authors	Muangpaisarn, P

Title	MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal	Molecular Cancer. 2015.
Authors	David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit

Midi (56100)

Title	Comparative Analysis of Free-Circulating and Vesicle-Associated Plasma microRNAs of Healthy Controls and Early-Stage Lung Cancer Patients
Journal	Pharmaceutics. 2022.
Authors	Luigi Pasini, Ivan Vannini, Paola Ulivi, Michela Tebaldi, Elisabetta Petracci, Francesco Fabbri, Franco Stella and Milena Urbini

Title	Circulating MicroRNAs: Association with Lung Function in Asthma
Journal	PLOS ONE. 2016.
Authors	Kho AT, Sharma S, Davis J, Spina J, Howard D, McEnroy K, Moore K, Sylvia J, Qiu W, Weiss ST, Tantisira KG

Maxi (56200)

Title	Identifying Candidate Circulating RNA Markers for Coronary Artery Disease by Deep RNA-Sequencing in Human Plasma
Journal	Cells. 2022.
Authors	Zoe Ward, Sebastian Schmeier, John Pearson, Vicky A Cameron, Chris M Frampton, Richard W Troughton, Rob N Doughty, A. Mark Richards, and Anna P Pilbrow.

Title	Circulating nucleic acids damage DNA of healthy cells by integrating into their genomes
Journal	Journal of Biosciences. 2015.
Authors	Mittra I, Khare NK, Raghuram GV, Chaubal R, Khambatti F, Gupta D, Gaikwad A, Prasannan P, Singh A, Iyer A, Singh A, Upadhyay P, Nair NK, Mishra PK, Dutt A.

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Plasma/Serum RNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

Plasma/Serum RNA Purification Mini Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL.

Plasma/Serum RNA Purification Midi Kit

This utilizes a two column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Plasma/Serum RNA Purification Maxi Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Isolate RNA after Purifying EVs and Exosomes

For Ultracentrifugation, Exoquick, and Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 - 25 µL	50 µL - 1 mL	250 µL - 1 mL	5 - 10 mL
35300	Total RNA Purification Micro Kit	20 - 50 µL	1 - 4 mL	2 - 10 mL	10 - 20 mL
17200	Total RNA Purification Kit	50 - 100 µL	4 - 10 mL	11 - 30 mL	20 - 35 mL

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 55000

Kit Specifications
Sample Volume Range	50 to 200 μL
Anti-coagulant (for Plasma)*	EDTA or Citrate
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Minimum Elution Volume	10 μL
Maximum Elution Volume	25 μL
Time to Complete 10 Purifications	15-20 minutes
Average Yield**	Variable depending on specimen

**Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability

You have selected: Cat. 56100

Kit Specifications
Minimum Plasma/Serum Input	250 μL
Maximum Plasma/Serum Input	1.5 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields*	Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

You have selected: Cat. 56200

Kit Specifications
Minimum Plasma/Serum Input	2 mL
Maximum Plasma/Serum Input	5 mL
Size of DNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields*	Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

Protocols:

(55000, 56100, 56200) Plasma/Serum RNA Purification Kits - Protocol (50 Preps, 20 Preps, 10 Preps)

Supplementary Protocols:

Plasma/Serum RNA Purification Mini Kit - Supplementary Protocol for Exosomal RNA Purification from Exosomes Already Purified

Plasma/Serum RNA Purification Mini Kit - Supplementary Protocol for miRNA Isolation from ExoCompleteTM 96-Well Plate Kit

Application Notes:

Plasma Exosomal microRNA Purification from Hitachi’s ExoComplete™ 96-Well Plate Kit

Safety Data Sheets:

(55000) Plasma/Serum RNA Purification Mini Kit - Safety Data Sheet

(56100, 56200) Plasma/Serum RNA Purification Midi/Maxi Kit - Safety Data Sheet

Product Flyers:

Plasma/Serum RNA Purification Kits

Mini (55000)

Title	Evidence for Effects of Extracellular Vesicles on Physical, Inflammatory, Transcriptome and Reward Behaviour Status in Mice
Journal	Int. J. Mol. Sci. 2022
Authors	Nagiua Cuomo-Haymour, Hannes Sigrist, Christian Ineichen, Giancarlo Russo, Ursina Nüesch, Felix Gantenbein, Luka Kulic, Irene Knuesel, Giorgio Bergamini and Christopher Robert Pryce

Title	Identification and characterization of extracellular vesicles from red cells infected with Babesia divergens and Babesia microti
Journal	Front. Cell. Infect. Microbiol. 2022
Authors	Divya Beri, Marilis Rodriguez, Manpreet Singh, Yunfeng Liu, Giselle Rasquinha, Xiuli An, Karina Yazdanbakhsh and Cheryl A. Lobo

Title	Clinicopathological utility of miR-203a-3p in diagnosing colorectal cancer
Journal	Mol Biol Rep. 2022
Authors	Mahdi Qasemi Rad, Vahid Pouresmaeil, Fatemeh Hosseini Mojahed, Amir Amirabadi, Amir Hossein Aalami

Title	Lower miR-21/ROS/HNE levels associate with lower glycemia after habit-intervention: DIAPASON study 1-year later
Journal	Cardiovascular Diabetology. 2022
Authors	Lucia La Sala, Elena Tagliabue, Simona Mrakic-Sposta, Anna Chiara Uccellatore, Pamela Senesi, Ileana Terruzzi, Emilio Trabucchi, Luigi Rossi-Bernardi & Livio Luzi

Title	The clinical utility of cfRNA for disease detection and surveillance: A proof of concept study in non-small cell lung cancer
Journal	Thoracic Cancer. 2022
Authors	Martin Metzenmacher, Balazs Hegedüs, Jan Forster,Alexander Schramm, Peter A. Horn, Christoph A. Klein, Nicola Bielefeld, Till Ploenes, Clemens Aigner, Jens T. Siveke, Martin Schuler, Smiths S. Lueong

Title	Differential Expression of Serum Extracellular Vesicle miRNAs in Multiple Sclerosis: Disease-Stage Specificity and Relevance to Pathophysiology
Journal	Int. J. Mol. Sci. 2022
Authors	Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce

Title	Differential expression of serum extracellular vesicle microRNAs and analysis of target-gene pathways in major depressive disorder
Journal	Biomarkers in Neuropsychiatry. 2022
Authors	Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce

Title	Predictive capacity of a miRNA panel in identifying teratoma in post-chemotherapy consolidation surgeries
Journal	BJUI International. 2022
Authors	Joseph A. Moore,Michael J. Lehner,Simone Anfossi,Saumil Datar,Rebecca S. Tidwell,Matthew Campbell,Amishi Y. Shah,John F. Ward,Jose A. Karam,Christopher G. Wood,Lois L. Pisters,George A. Calin,Shi-Ming Tu

Title	Downregulation of Talin‐1 is associated with the increased expression of miR‐182‐5p and miR‐9‐5p in coronary artery disease
Journal	J Clin Lab Anal. 2022
Authors	Akram Gholipour, Farshad Shakerian, Ali Zahedmehr, Shiva Irani, Seyed Javad Mowla, and Mahshid Malakootian

Title	miR3-22-p as a Novel Biomarker in Rheumatoid Arthritis
Journal	Medicine Personalized Journal. 2022
Authors	Maryam Hassanpoor, Bahareh Abbasi

Title	Increased expression of miR-224-5p in circulating extracellular vesicles of patients with reduced coronary flow reserve
Journal	BMC Cardiovascular Disorders. 2022
Authors	Kreema James, Paulina Bryl-Gorecka, Björn Olde, Olof Gidlof, Kristina Torngren & David Erlinge

Title	Variability in Cerebrospinal Fluid MicroRNAs Through Life
Journal	Molecular Neurobiology. 2020
Authors	Prieto-Fernández, Endika, et al.

Title	Serum microRNA-34a is potential biomarker for inflammation in nonalcoholic fatty liver disease
Journal	Asian Biomedicine. 2016.
Authors	Muangpaisarn, P

Title	MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal	Molecular Cancer. 2015.
Authors	David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit

Midi (56100)

Title	Comparative Analysis of Free-Circulating and Vesicle-Associated Plasma microRNAs of Healthy Controls and Early-Stage Lung Cancer Patients
Journal	Pharmaceutics. 2022.
Authors	Luigi Pasini, Ivan Vannini, Paola Ulivi, Michela Tebaldi, Elisabetta Petracci, Francesco Fabbri, Franco Stella and Milena Urbini

Title	Circulating MicroRNAs: Association with Lung Function in Asthma
Journal	PLOS ONE. 2016.
Authors	Kho AT, Sharma S, Davis J, Spina J, Howard D, McEnroy K, Moore K, Sylvia J, Qiu W, Weiss ST, Tantisira KG

Maxi (56200)

Title	Identifying Candidate Circulating RNA Markers for Coronary Artery Disease by Deep RNA-Sequencing in Human Plasma
Journal	Cells. 2022.
Authors	Zoe Ward, Sebastian Schmeier, John Pearson, Vicky A Cameron, Chris M Frampton, Richard W Troughton, Rob N Doughty, A. Mark Richards, and Anna P Pilbrow.

Title	Circulating nucleic acids damage DNA of healthy cells by integrating into their genomes
Journal	Journal of Biosciences. 2015.
Authors	Mittra I, Khare NK, Raghuram GV, Chaubal R, Khambatti F, Gupta D, Gaikwad A, Prasannan P, Singh A, Iyer A, Singh A, Upadhyay P, Nair NK, Mishra PK, Dutt A.

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Plasma/Serum RNA Purification Kits (Cat. 55000, 56100, 56200)

Plasma/Serum RNA Purification Kits

Plasma/Serum RNA Purification Kits

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Request a Product Sample

Related Services

NGS

RNA Isolation

Related Products

microRNA (cel-miR-39) Spike-In Kit

Plasma/Serum Cell-Free Circulating DNA Purification Kits

Plasma/Serum RNA Purification Kits (Cat. 55000, 56100, 56200)

Plasma/Serum RNA Purification Kits

Plasma/Serum RNA Purification Kits

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Request a Product Sample

Product Overview

Documentation

Citations

Related Services

NGS

RNA Isolation

Related Products

microRNA (cel-miR-39) Spike-In Kit

Plasma/Serum Cell-Free Circulating DNA Purification Kits

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.