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Plasma/Serum RNA Purification Kits

Cat. 55000, 56100, 56200
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Plasma/Serum RNA Purification Kits

Cat. 55000, 56100, 56200

For rapid and simple purification of circulating RNA and Exosomal RNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma/serum input ranges
  • No phenol extractions
  • No carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into a flexible elution volume
  • High quality, purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Compatible with Streck Cell-Free RNA BCT® Tubes
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Plasma/Serum RNA Purification Kit
(Cat. 55000)
50 preps
Plasma/Serum RNA Purification Midi Kit
(Cat. 56100)
20 preps
Plasma/Serum RNA Purification Maxi Kit
(Cat. 56200)
10 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

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Please note that Norgen Biotek provides sample kits exclusively to residents of North America. Customers outside of North America may request a sample from one of our global distributors. Find your local distributor.

Kit Specifications

You have selected: Cat. 55000
Kit Specifications
Sample Volume Range
50 to 200 μL
Anti-coagulant (for Plasma)*
EDTA or Citrate
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Minimum Elution Volume
10 μL
Maximum Elution Volume 25 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yield**
Variable depending on specimen

*This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

**Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

 

You have selected: Cat. 56100
Kit Specifications
Minimum Plasma/Serum Input
250 μL
Maximum Plasma/Serum Input
1.5 mL
Size of RNA Purified
All sizes, including miRNA
and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 56200
Kit Specifications
Minimum Plasma/Serum Input
2 mL
Maximum Plasma/Serum Input
5 mL
Size of DNA Purified
All sizes, including miRNA
and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Supporting Data

Click for expanded view

Mini (55000)

Title Evidence for Effects of Extracellular Vesicles on Physical, Inflammatory, Transcriptome and Reward Behaviour Status in Mice
Journal Int. J. Mol. Sci. 2022
Authors Nagiua Cuomo-Haymour, Hannes Sigrist, Christian Ineichen, Giancarlo Russo, Ursina Nüesch, Felix Gantenbein, Luka Kulic, Irene Knuesel, Giorgio Bergamini and Christopher Robert Pryce
Title Identification and characterization of extracellular vesicles from red cells infected with Babesia divergens and Babesia microti
Journal Front. Cell. Infect. Microbiol. 2022
Authors Divya Beri, Marilis Rodriguez, Manpreet Singh, Yunfeng Liu, Giselle Rasquinha, Xiuli An, Karina Yazdanbakhsh and Cheryl A. Lobo
Title Clinicopathological utility of miR-203a-3p in diagnosing colorectal cancer
Journal Mol Biol Rep. 2022
Authors Mahdi Qasemi Rad, Vahid Pouresmaeil, Fatemeh Hosseini Mojahed, Amir Amirabadi, Amir Hossein Aalami
Title Lower miR-21/ROS/HNE levels associate with lower glycemia after habit-intervention: DIAPASON study 1-year later
Journal Cardiovascular Diabetology. 2022
Authors Lucia La Sala, Elena Tagliabue, Simona Mrakic-Sposta, Anna Chiara Uccellatore, Pamela Senesi, Ileana Terruzzi, Emilio Trabucchi, Luigi Rossi-Bernardi & Livio Luzi
Title The clinical utility of cfRNA for disease detection and surveillance: A proof of concept study in non-small cell lung cancer
Journal Thoracic Cancer. 2022
Authors Martin Metzenmacher, Balazs Hegedüs, Jan Forster,Alexander Schramm, Peter A. Horn, Christoph A. Klein, Nicola Bielefeld, Till Ploenes, Clemens Aigner, Jens T. Siveke, Martin Schuler, Smiths S. Lueong
Title Differential Expression of Serum Extracellular Vesicle miRNAs in Multiple Sclerosis: Disease-Stage Specificity and Relevance to Pathophysiology
Journal Int. J. Mol. Sci. 2022
Authors Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce
Title Differential expression of serum extracellular vesicle microRNAs and analysis of target-gene pathways in major depressive disorder
Journal Biomarkers in Neuropsychiatry. 2022
Authors Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce
Title Predictive capacity of a miRNA panel in identifying teratoma in post-chemotherapy consolidation surgeries
Journal BJUI International. 2022
Authors Joseph A. Moore,Michael J. Lehner,Simone Anfossi,Saumil Datar,Rebecca S. Tidwell,Matthew Campbell,Amishi Y. Shah,John F. Ward,Jose A. Karam,Christopher G. Wood,Lois L. Pisters,George A. Calin,Shi-Ming Tu
Title Downregulation of Talin‐1 is associated with the increased expression of miR‐182‐5p and miR‐9‐5p in coronary artery disease
Journal J Clin Lab Anal. 2022
Authors Akram Gholipour, Farshad Shakerian, Ali Zahedmehr, Shiva Irani, Seyed Javad Mowla, and Mahshid Malakootian
Title miR3-22-p as a Novel Biomarker in Rheumatoid Arthritis
Journal Medicine Personalized Journal. 2022
Authors Maryam Hassanpoor, Bahareh Abbasi
Title Increased expression of miR-224-5p in circulating extracellular vesicles of patients with reduced coronary flow reserve
Journal BMC Cardiovascular Disorders. 2022
Authors Kreema James, Paulina Bryl-Gorecka, Björn Olde, Olof Gidlof, Kristina Torngren & David Erlinge
Title Variability in Cerebrospinal Fluid MicroRNAs Through Life
Journal Molecular Neurobiology. 2020
Authors Prieto-Fernández, Endika, et al.
Title Serum microRNA-34a is potential biomarker for inflammation in nonalcoholic fatty liver disease
Journal Asian Biomedicine. 2016.
Authors Muangpaisarn, P
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit

Midi (56100)

Title Comparative Analysis of Free-Circulating and Vesicle-Associated Plasma microRNAs of Healthy Controls and Early-Stage Lung Cancer Patients
Journal Pharmaceutics. 2022.
Authors Luigi Pasini, Ivan Vannini, Paola Ulivi, Michela Tebaldi, Elisabetta Petracci, Francesco Fabbri, Franco Stella and Milena Urbini
Title Circulating MicroRNAs: Association with Lung Function in Asthma
Journal PLOS ONE. 2016.
Authors Kho AT, Sharma S, Davis J, Spina J, Howard D, McEnroy K, Moore K, Sylvia J, Qiu W, Weiss ST, Tantisira KG

Maxi (56200)

Title Identifying Candidate Circulating RNA Markers for Coronary Artery Disease by Deep RNA-Sequencing in Human Plasma
Journal Cells. 2022.
Authors Zoe Ward, Sebastian Schmeier, John Pearson, Vicky A Cameron, Chris M Frampton, Richard W Troughton, Rob N Doughty, A. Mark Richards, and Anna P Pilbrow.
Title Circulating nucleic acids damage DNA of healthy cells by integrating into their genomes
Journal Journal of Biosciences. 2015.
Authors Mittra I, Khare NK, Raghuram GV, Chaubal R, Khambatti F, Gupta D, Gaikwad A, Prasannan P, Singh A, Iyer A, Singh A, Upadhyay P, Nair NK, Mishra PK, Dutt A.

Plasma/Serum RNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

Plasma/Serum RNA Purification Mini Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL.

Plasma/Serum RNA Purification Midi Kit

This utilizes a two column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Plasma/Serum RNA Purification Maxi Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Isolate RNA after Purifying EVs and Exosomes

For Ultracentrifugation, Exoquick, and Filtration

Cat. # Name Elution Volume Plasma/Serum Urine Cell-Culture Media
55000 Plasma/Serum RNA Purification Mini Kit 10 - 25 µL 50 µL - 1 mL 250 µL - 1 mL 5 - 10 mL
35300 Total RNA Purification Micro Kit 20 - 50 µL 1 - 4 mL 2 - 10 mL 10 - 20 mL
17200 Total RNA Purification Kit 50 - 100 µL 4 - 10 mL 11 - 30 mL 20 - 35 mL

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 55000
Kit Specifications
Sample Volume Range
50 to 200 μL
Anti-coagulant (for Plasma)*
EDTA or Citrate
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Minimum Elution Volume
10 μL
Maximum Elution Volume 25 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yield**
Variable depending on specimen

*This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

**Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

 

You have selected: Cat. 56100
Kit Specifications
Minimum Plasma/Serum Input
250 μL
Maximum Plasma/Serum Input
1.5 mL
Size of RNA Purified
All sizes, including miRNA
and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 56200
Kit Specifications
Minimum Plasma/Serum Input
2 mL
Maximum Plasma/Serum Input
5 mL
Size of DNA Purified
All sizes, including miRNA
and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Mini (55000)

Title Evidence for Effects of Extracellular Vesicles on Physical, Inflammatory, Transcriptome and Reward Behaviour Status in Mice
Journal Int. J. Mol. Sci. 2022
Authors Nagiua Cuomo-Haymour, Hannes Sigrist, Christian Ineichen, Giancarlo Russo, Ursina Nüesch, Felix Gantenbein, Luka Kulic, Irene Knuesel, Giorgio Bergamini and Christopher Robert Pryce
Title Identification and characterization of extracellular vesicles from red cells infected with Babesia divergens and Babesia microti
Journal Front. Cell. Infect. Microbiol. 2022
Authors Divya Beri, Marilis Rodriguez, Manpreet Singh, Yunfeng Liu, Giselle Rasquinha, Xiuli An, Karina Yazdanbakhsh and Cheryl A. Lobo
Title Clinicopathological utility of miR-203a-3p in diagnosing colorectal cancer
Journal Mol Biol Rep. 2022
Authors Mahdi Qasemi Rad, Vahid Pouresmaeil, Fatemeh Hosseini Mojahed, Amir Amirabadi, Amir Hossein Aalami
Title Lower miR-21/ROS/HNE levels associate with lower glycemia after habit-intervention: DIAPASON study 1-year later
Journal Cardiovascular Diabetology. 2022
Authors Lucia La Sala, Elena Tagliabue, Simona Mrakic-Sposta, Anna Chiara Uccellatore, Pamela Senesi, Ileana Terruzzi, Emilio Trabucchi, Luigi Rossi-Bernardi & Livio Luzi
Title The clinical utility of cfRNA for disease detection and surveillance: A proof of concept study in non-small cell lung cancer
Journal Thoracic Cancer. 2022
Authors Martin Metzenmacher, Balazs Hegedüs, Jan Forster,Alexander Schramm, Peter A. Horn, Christoph A. Klein, Nicola Bielefeld, Till Ploenes, Clemens Aigner, Jens T. Siveke, Martin Schuler, Smiths S. Lueong
Title Differential Expression of Serum Extracellular Vesicle miRNAs in Multiple Sclerosis: Disease-Stage Specificity and Relevance to Pathophysiology
Journal Int. J. Mol. Sci. 2022
Authors Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce
Title Differential expression of serum extracellular vesicle microRNAs and analysis of target-gene pathways in major depressive disorder
Journal Biomarkers in Neuropsychiatry. 2022
Authors Nagiua Cuomo-Haymour, Stefan Kaiser, Matthias Hartmann-Riemer, Karoline Guetter, Federica Klaus, Flurin Cathomas, Erich Seifritz, Giorgio Bergamini, Giancarlo Russoc, Christopher R.Pryce
Title Predictive capacity of a miRNA panel in identifying teratoma in post-chemotherapy consolidation surgeries
Journal BJUI International. 2022
Authors Joseph A. Moore,Michael J. Lehner,Simone Anfossi,Saumil Datar,Rebecca S. Tidwell,Matthew Campbell,Amishi Y. Shah,John F. Ward,Jose A. Karam,Christopher G. Wood,Lois L. Pisters,George A. Calin,Shi-Ming Tu
Title Downregulation of Talin‐1 is associated with the increased expression of miR‐182‐5p and miR‐9‐5p in coronary artery disease
Journal J Clin Lab Anal. 2022
Authors Akram Gholipour, Farshad Shakerian, Ali Zahedmehr, Shiva Irani, Seyed Javad Mowla, and Mahshid Malakootian
Title miR3-22-p as a Novel Biomarker in Rheumatoid Arthritis
Journal Medicine Personalized Journal. 2022
Authors Maryam Hassanpoor, Bahareh Abbasi
Title Increased expression of miR-224-5p in circulating extracellular vesicles of patients with reduced coronary flow reserve
Journal BMC Cardiovascular Disorders. 2022
Authors Kreema James, Paulina Bryl-Gorecka, Björn Olde, Olof Gidlof, Kristina Torngren & David Erlinge
Title Variability in Cerebrospinal Fluid MicroRNAs Through Life
Journal Molecular Neurobiology. 2020
Authors Prieto-Fernández, Endika, et al.
Title Serum microRNA-34a is potential biomarker for inflammation in nonalcoholic fatty liver disease
Journal Asian Biomedicine. 2016.
Authors Muangpaisarn, P
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit

Midi (56100)

Title Comparative Analysis of Free-Circulating and Vesicle-Associated Plasma microRNAs of Healthy Controls and Early-Stage Lung Cancer Patients
Journal Pharmaceutics. 2022.
Authors Luigi Pasini, Ivan Vannini, Paola Ulivi, Michela Tebaldi, Elisabetta Petracci, Francesco Fabbri, Franco Stella and Milena Urbini
Title Circulating MicroRNAs: Association with Lung Function in Asthma
Journal PLOS ONE. 2016.
Authors Kho AT, Sharma S, Davis J, Spina J, Howard D, McEnroy K, Moore K, Sylvia J, Qiu W, Weiss ST, Tantisira KG

Maxi (56200)

Title Identifying Candidate Circulating RNA Markers for Coronary Artery Disease by Deep RNA-Sequencing in Human Plasma
Journal Cells. 2022.
Authors Zoe Ward, Sebastian Schmeier, John Pearson, Vicky A Cameron, Chris M Frampton, Richard W Troughton, Rob N Doughty, A. Mark Richards, and Anna P Pilbrow.
Title Circulating nucleic acids damage DNA of healthy cells by integrating into their genomes
Journal Journal of Biosciences. 2015.
Authors Mittra I, Khare NK, Raghuram GV, Chaubal R, Khambatti F, Gupta D, Gaikwad A, Prasannan P, Singh A, Iyer A, Singh A, Upadhyay P, Nair NK, Mishra PK, Dutt A.

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