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RNase-Free DNase I Kit (Cat. 25710, 25720)

 

Purchasing Information

  • Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
  • Also includes protocol for digestion in-solution followed by RNA Clean-Up
  • Guaranteed RNase-Free
  • Includes Enzyme Incubation Buffer
  • Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

DNase I Kits - 25% off combo special! Click here for details.

For DNase digestion during RNA purification either on-column or in-solution

ItemsCat. #Size
RNase-Free DNase I Kit 25710 50 reactions
RNase-Free DNase I Kit 25720 200 reactions

  • Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
  • Also includes protocol for digestion in-solution followed by RNA Clean-Up
  • Guaranteed RNase-Free
  • Includes Enzyme Incubation Buffer
  • Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)


RNase-Free DNase I Kit

Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.


Title Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages
Journal Translational Psychiatry. 2017.
Authors Vizlin-Hodzic D, Zhai Q, Illes S,, Södersten K, Truvé K, Parris TZ, Sobhan PK, Salmela S, Kosalai ST, Kanduri C, Strandberg J, Seth H, Bontell TO,, Hanse E, Ågren H, Funa K
Title Complete Transcriptome RNA-Seq
Journal Cancer Gene Networks. 2016.
Authors David F. B. Miller, Pearlly Yan, Fang Fang, Aaron Buechlein, Karl Kroll, David Frankhouser, Cameron Stump, Paige Stump, James B. Ford, Haixu Tang, Scott Michaels, Daniela Matei, Tim H. Huang, Jeremy Chien, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew
Title Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974.
Journal Laboratory Investigation. 2015.
Authors Boone JD, Arend RC, Johnston BE, Cooper SJ, Gilchrist SA, Oelschlager DK, Grizzle WE, McGwin G Jr, Gangrade A, Straughn JM Jr, Buchsbaum DJ
Title The Molecular and Genetic Basis of Repeatable Coevolution between Escherichia coli and Bacteriophage T3 in a Laboratory Microcosm
Journal PLOS ONE. 2015.
Authors Elizabeth B. Perry, Jeffrey E. Barrick, Brendan J. M. Bohannan
Title Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung
Journal Development. 2015.
Authors Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat
Title Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources
Journal Archives of Microbiology. 2014.
Authors Anna Casabianca, Chiara Orlandi, Federica Barbieri, Luigia Sabatini, Andrea Di Cesare, Davide Sisti, Sonia Pasquaroli, Mauro Magnani, Barbara Citterio
Title Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks.
Journal Neuropeptides. 2014.
Authors Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline.
Title Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks.
Journal Neuropeptides. 2014.
Authors Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline.
Title Molecules Altering the Intracellular Thiol Content Modulate NF-kB and STAT-1/IRF-1 Signalling Pathways and IL-12 p40 and IL-27 p28 Production in Murine Macrophages.
Journal PLoS One. 2013.
Authors Fraternale A, Crinelli R, Casabianca A, Paoletti MF, Orlandi C, Carloni E, Smietana M, Palamara AT, Magnani M.

ItemsCat. #Size
RNase-Free DNase I Kit 25710 50 reactions
RNase-Free DNase I Kit 25720 200 reactions

  • Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
  • Also includes protocol for digestion in-solution followed by RNA Clean-Up
  • Guaranteed RNase-Free
  • Includes Enzyme Incubation Buffer
  • Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)


RNase-Free DNase I Kit

Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Title Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages
Journal Translational Psychiatry. 2017.
Authors Vizlin-Hodzic D, Zhai Q, Illes S,, Södersten K, Truvé K, Parris TZ, Sobhan PK, Salmela S, Kosalai ST, Kanduri C, Strandberg J, Seth H, Bontell TO,, Hanse E, Ågren H, Funa K
Title Complete Transcriptome RNA-Seq
Journal Cancer Gene Networks. 2016.
Authors David F. B. Miller, Pearlly Yan, Fang Fang, Aaron Buechlein, Karl Kroll, David Frankhouser, Cameron Stump, Paige Stump, James B. Ford, Haixu Tang, Scott Michaels, Daniela Matei, Tim H. Huang, Jeremy Chien, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew
Title Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974.
Journal Laboratory Investigation. 2015.
Authors Boone JD, Arend RC, Johnston BE, Cooper SJ, Gilchrist SA, Oelschlager DK, Grizzle WE, McGwin G Jr, Gangrade A, Straughn JM Jr, Buchsbaum DJ
Title The Molecular and Genetic Basis of Repeatable Coevolution between Escherichia coli and Bacteriophage T3 in a Laboratory Microcosm
Journal PLOS ONE. 2015.
Authors Elizabeth B. Perry, Jeffrey E. Barrick, Brendan J. M. Bohannan
Title Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung
Journal Development. 2015.
Authors Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat
Title Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources
Journal Archives of Microbiology. 2014.
Authors Anna Casabianca, Chiara Orlandi, Federica Barbieri, Luigia Sabatini, Andrea Di Cesare, Davide Sisti, Sonia Pasquaroli, Mauro Magnani, Barbara Citterio
Title Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks.
Journal Neuropeptides. 2014.
Authors Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline.
Title Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks.
Journal Neuropeptides. 2014.
Authors Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline.
Title Molecules Altering the Intracellular Thiol Content Modulate NF-kB and STAT-1/IRF-1 Signalling Pathways and IL-12 p40 and IL-27 p28 Production in Murine Macrophages.
Journal PLoS One. 2013.
Authors Fraternale A, Crinelli R, Casabianca A, Paoletti MF, Orlandi C, Carloni E, Smietana M, Palamara AT, Magnani M.

 

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