For DNase digestion during RNA purification either on-column or in-solution
|RNase-Free DNase I Kit||25710||50 reactions|
|RNase-Free DNase I Kit||25720||200 reactions|
RNase-Free DNase I Kit
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
|Title||Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages|
|Journal||Translational Psychiatry. 2017.|
|Authors||Vizlin-Hodzic D, Zhai Q, Illes S,, Södersten K, Truvé K, Parris TZ, Sobhan PK, Salmela S, Kosalai ST, Kanduri C, Strandberg J, Seth H, Bontell TO,, Hanse E, Ågren H, Funa K|
|Title||Complete Transcriptome RNA-Seq|
|Journal||Cancer Gene Networks. 2016.|
|Authors||David F. B. Miller, Pearlly Yan, Fang Fang, Aaron Buechlein, Karl Kroll, David Frankhouser, Cameron Stump, Paige Stump, James B. Ford, Haixu Tang, Scott Michaels, Daniela Matei, Tim H. Huang, Jeremy Chien, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew|
|Title||Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974.|
|Journal||Laboratory Investigation. 2015.|
|Authors||Boone JD, Arend RC, Johnston BE, Cooper SJ, Gilchrist SA, Oelschlager DK, Grizzle WE, McGwin G Jr, Gangrade A, Straughn JM Jr, Buchsbaum DJ|
|Title||The Molecular and Genetic Basis of Repeatable Coevolution between Escherichia coli and Bacteriophage T3 in a Laboratory Microcosm|
|Journal||PLOS ONE. 2015.|
|Authors||Elizabeth B. Perry, Jeffrey E. Barrick, Brendan J. M. Bohannan|
|Title||Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung|
|Authors||Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat|
|Title||Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources|
|Journal||Archives of Microbiology. 2014.|
|Authors||Anna Casabianca, Chiara Orlandi, Federica Barbieri, Luigia Sabatini, Andrea Di Cesare, Davide Sisti, Sonia Pasquaroli, Mauro Magnani, Barbara Citterio|
|Title||Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks.|
|Authors||Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline.|
|Title||Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks.|
|Authors||Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline.|
|Title||Molecules Altering the Intracellular Thiol Content Modulate NF-kB and STAT-1/IRF-1 Signalling Pathways and IL-12 p40 and IL-27 p28 Production in Murine Macrophages.|
|Journal||PLoS One. 2013.|
|Authors||Fraternale A, Crinelli R, Casabianca A, Paoletti MF, Orlandi C, Carloni E, Smietana M, Palamara AT, Magnani M.|
Copyright © 2019 - Norgen Biotek Corp.