RNase-Free DNase I Kit (Cat. 25710, 25720)
RNase-Free DNase I Kit
![Norgen Biotek RNase-Free DNase I Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/25710.png?itok=JlE1Kpel" "RNase-Free DNase I Kit")
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RNase-Free DNase I Kit
For DNase digestion during RNA purification either on-column or in-solution
- Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
- Also includes protocol for digestion in-solution followed by RNA Clean-Up
- Guaranteed RNase-Free
- Includes Enzyme Incubation Buffer
- Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 25710) 50 reactions
(Cat. 25720) 200 reactions
Kit Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Kit Size
- Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
- Also includes protocol for digestion in-solution followed by RNA Clean-Up
- Guaranteed RNase-Free
- Includes Enzyme Incubation Buffer
- Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)
RNase-Free DNase I Kit
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Kit Specifications
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
| Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
|---|---|---|
| DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
| Buffer DR | 1 mL | 4 x 1 mL |
| Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
| Product Insert | 1 | 1 |
| Title | MicroRNA-574 Impacts Granulosa Cell Estradiol Production via Targeting TIMP3 and ERK1/2 Signaling Pathway |
| Journal | Front Endocrinol (Lausanne). 2022. |
| Authors | Bo Pan, Xiaoshu Zhan, Julang Li |
| Title | Investigation of the direct effect of a precision Ascophyllum nodosum biostimulant on nitrogen use efficiency in wheat seedlings |
| Journal | Plant Physiology and Biochemistry 2022. |
| Authors | Łukasz Łangowski, Oscar Goñi, Elomofe Ikuyinminu, Ewan Feeney, Shane O' Connell |
| Title | Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species |
| Journal | Chromosome Research. 2022. |
| Authors | Anastassia Boudichevskaia, Anne Fiebig, Katrin Kumke, Axel Himmelbach & Andreas Houben |
| Title | A multi-omics approach to visualize early neuronal differentiation in 4D |
| Journal | bioRxiv. 2022. |
| Authors | Athina Samara, Mari Spildrejorde, Ankush Sharma, Martin Falck,Magnus Leithaug, Stefania Modafferi, Pål Marius Bjørnstad, Ganesh Acharya, Kristina Gervin, Robert Lyle and Ragnhild Eskeland |
| Title | APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms |
| Journal | Int. J. Mol. Sci. 2022 |
| Authors | Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, and Sarah Vreugde |
| Title | Interferon Tau (IFNt) and Interferon-Stimulated Genes (ISGs) Expression in Peripheral Blood Leukocytes and Correlation with Circulating Pregnancy-Associated Glycoproteins (PAGs) during Peri-Implantation and Early Pregnancy in Buffalo Cows |
| Journal | Animals 2022. |
| Authors | Anna Beatrice Casano, Vittoria Lucia Barile, Laura Menchetti, Gabriella Guelfi, Gabriele Brecchia, Stella Agradi, Giovanna De Matteis, Maria Carmela Scatà, Francesco Grandoni and Olimpia Barbato |
| Title | Functional genetic screen identifies ITPR3/calcium/ RELB axis as a driver of colorectal cancer metastatic liver colonization |
| Journal | Developmental Cell 2022. |
| Authors | Ryan H. Moy, Alexander Nguyen, Jia Min Loo, Y. Gloria Wu, Saeed Tavazoie, Sohail F. Tavazoie |
| Title | Assessing the Morphological and Behavioral Toxicity of Catechol Using Larval Zebrafish |
| Journal | Int. J. Mol. Sci. 2022. |
| Authors | Michael G. Morash, Kelly H. Soanes, John C. Achenbach and Lee D. Ellis |
| Title | Vitamin D and interferon-γ co-operate to increase the ACE-2 receptor expression in primary cultures of human thyroid cells |
| Journal | Journal of Endocrinological Investigation. 2022. |
| Authors | F. Coperchini, A. Greco, M. Denegri, F. A. Ripepi, B. Grillini, J. Bertini, B. Calì, L. Villani, F. Magri, L. Croce, C. Gaetano, C. Cappelli, P. Trimboli, L. Chiovato & M. Rotondi |
| Title | Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages |
| Journal | Translational Psychiatry. 2017. |
| Authors | Vizlin-Hodzic D, Zhai Q, Illes S,, Södersten K, Truvé K, Parris TZ, Sobhan PK, Salmela S, Kosalai ST, Kanduri C, Strandberg J, Seth H, Bontell TO,, Hanse E, Ågren H, Funa K |
| Title | Complete Transcriptome RNA-Seq |
| Journal | Cancer Gene Networks. 2016. |
| Authors | David F. B. Miller, Pearlly Yan, Fang Fang, Aaron Buechlein, Karl Kroll, David Frankhouser, Cameron Stump, Paige Stump, James B. Ford, Haixu Tang, Scott Michaels, Daniela Matei, Tim H. Huang, Jeremy Chien, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew |
| Title | Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974. |
| Journal | Laboratory Investigation. 2015. |
| Authors | Boone JD, Arend RC, Johnston BE, Cooper SJ, Gilchrist SA, Oelschlager DK, Grizzle WE, McGwin G Jr, Gangrade A, Straughn JM Jr, Buchsbaum DJ |
| Title | The Molecular and Genetic Basis of Repeatable Coevolution between Escherichia coli and Bacteriophage T3 in a Laboratory Microcosm |
| Journal | PLOS ONE. 2015. |
| Authors | Elizabeth B. Perry, Jeffrey E. Barrick, Brendan J. M. Bohannan |
| Title | Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung |
| Journal | Development. 2015. |
| Authors | Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat |
| Title | Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources |
| Journal | Archives of Microbiology. 2014. |
| Authors | Anna Casabianca, Chiara Orlandi, Federica Barbieri, Luigia Sabatini, Andrea Di Cesare, Davide Sisti, Sonia Pasquaroli, Mauro Magnani, Barbara Citterio |
| Title | Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline. |
| Title | Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline. |
| Title | Molecules Altering the Intracellular Thiol Content Modulate NF-kB and STAT-1/IRF-1 Signalling Pathways and IL-12 p40 and IL-27 p28 Production in Murine Macrophages. |
| Journal | PLoS One. 2013. |
| Authors | Fraternale A, Crinelli R, Casabianca A, Paoletti MF, Orlandi C, Carloni E, Smietana M, Palamara AT, Magnani M. |
RNase-Free DNase I Kit
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Kit Specifications
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
| Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
|---|---|---|
| DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
| Buffer DR | 1 mL | 4 x 1 mL |
| Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
| Product Insert | 1 | 1 |
| Title | MicroRNA-574 Impacts Granulosa Cell Estradiol Production via Targeting TIMP3 and ERK1/2 Signaling Pathway |
| Journal | Front Endocrinol (Lausanne). 2022. |
| Authors | Bo Pan, Xiaoshu Zhan, Julang Li |
| Title | Investigation of the direct effect of a precision Ascophyllum nodosum biostimulant on nitrogen use efficiency in wheat seedlings |
| Journal | Plant Physiology and Biochemistry 2022. |
| Authors | Łukasz Łangowski, Oscar Goñi, Elomofe Ikuyinminu, Ewan Feeney, Shane O' Connell |
| Title | Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species |
| Journal | Chromosome Research. 2022. |
| Authors | Anastassia Boudichevskaia, Anne Fiebig, Katrin Kumke, Axel Himmelbach & Andreas Houben |
| Title | A multi-omics approach to visualize early neuronal differentiation in 4D |
| Journal | bioRxiv. 2022. |
| Authors | Athina Samara, Mari Spildrejorde, Ankush Sharma, Martin Falck,Magnus Leithaug, Stefania Modafferi, Pål Marius Bjørnstad, Ganesh Acharya, Kristina Gervin, Robert Lyle and Ragnhild Eskeland |
| Title | APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms |
| Journal | Int. J. Mol. Sci. 2022 |
| Authors | Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, and Sarah Vreugde |
| Title | Interferon Tau (IFNt) and Interferon-Stimulated Genes (ISGs) Expression in Peripheral Blood Leukocytes and Correlation with Circulating Pregnancy-Associated Glycoproteins (PAGs) during Peri-Implantation and Early Pregnancy in Buffalo Cows |
| Journal | Animals 2022. |
| Authors | Anna Beatrice Casano, Vittoria Lucia Barile, Laura Menchetti, Gabriella Guelfi, Gabriele Brecchia, Stella Agradi, Giovanna De Matteis, Maria Carmela Scatà, Francesco Grandoni and Olimpia Barbato |
| Title | Functional genetic screen identifies ITPR3/calcium/ RELB axis as a driver of colorectal cancer metastatic liver colonization |
| Journal | Developmental Cell 2022. |
| Authors | Ryan H. Moy, Alexander Nguyen, Jia Min Loo, Y. Gloria Wu, Saeed Tavazoie, Sohail F. Tavazoie |
| Title | Assessing the Morphological and Behavioral Toxicity of Catechol Using Larval Zebrafish |
| Journal | Int. J. Mol. Sci. 2022. |
| Authors | Michael G. Morash, Kelly H. Soanes, John C. Achenbach and Lee D. Ellis |
| Title | Vitamin D and interferon-γ co-operate to increase the ACE-2 receptor expression in primary cultures of human thyroid cells |
| Journal | Journal of Endocrinological Investigation. 2022. |
| Authors | F. Coperchini, A. Greco, M. Denegri, F. A. Ripepi, B. Grillini, J. Bertini, B. Calì, L. Villani, F. Magri, L. Croce, C. Gaetano, C. Cappelli, P. Trimboli, L. Chiovato & M. Rotondi |
| Title | Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages |
| Journal | Translational Psychiatry. 2017. |
| Authors | Vizlin-Hodzic D, Zhai Q, Illes S,, Södersten K, Truvé K, Parris TZ, Sobhan PK, Salmela S, Kosalai ST, Kanduri C, Strandberg J, Seth H, Bontell TO,, Hanse E, Ågren H, Funa K |
| Title | Complete Transcriptome RNA-Seq |
| Journal | Cancer Gene Networks. 2016. |
| Authors | David F. B. Miller, Pearlly Yan, Fang Fang, Aaron Buechlein, Karl Kroll, David Frankhouser, Cameron Stump, Paige Stump, James B. Ford, Haixu Tang, Scott Michaels, Daniela Matei, Tim H. Huang, Jeremy Chien, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew |
| Title | Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974. |
| Journal | Laboratory Investigation. 2015. |
| Authors | Boone JD, Arend RC, Johnston BE, Cooper SJ, Gilchrist SA, Oelschlager DK, Grizzle WE, McGwin G Jr, Gangrade A, Straughn JM Jr, Buchsbaum DJ |
| Title | The Molecular and Genetic Basis of Repeatable Coevolution between Escherichia coli and Bacteriophage T3 in a Laboratory Microcosm |
| Journal | PLOS ONE. 2015. |
| Authors | Elizabeth B. Perry, Jeffrey E. Barrick, Brendan J. M. Bohannan |
| Title | Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung |
| Journal | Development. 2015. |
| Authors | Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat |
| Title | Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources |
| Journal | Archives of Microbiology. 2014. |
| Authors | Anna Casabianca, Chiara Orlandi, Federica Barbieri, Luigia Sabatini, Andrea Di Cesare, Davide Sisti, Sonia Pasquaroli, Mauro Magnani, Barbara Citterio |
| Title | Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline. |
| Title | Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline. |
| Title | Molecules Altering the Intracellular Thiol Content Modulate NF-kB and STAT-1/IRF-1 Signalling Pathways and IL-12 p40 and IL-27 p28 Production in Murine Macrophages. |
| Journal | PLoS One. 2013. |
| Authors | Fraternale A, Crinelli R, Casabianca A, Paoletti MF, Orlandi C, Carloni E, Smietana M, Palamara AT, Magnani M. |
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