For rapid and simple purification of circulating RNA and Exosomal RNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma/serum input ranging from 50 µL to 200 µL
  • No phenol extractions
  • No carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 10 µL to 25 µL
  • Purify high-quality RNA in 15-20 minutes

ItemsCat. #Size
Plasma/Serum RNA Purification Kit 55000 50 preps

Plasma/Serum RNA Purification Mini Kit

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR.

The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL and is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

Kit Specifications
Minimum Plasma/Serum Input
50 μL
Maximum Plasma/Serum Input
200 μL
Size of RNA Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
10-25 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 year without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.


Kit Components
Component Cat. 55000 (50 preps)
Lysis Buffer A 30 mL
Wash Solution A 18 mL
Elution Solution A 6 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Serum microRNA-34a is potential biomarker for inflammation in nonalcoholic fatty liver disease
Journal Asian Biomedicine. 2016.
Authors Muangpaisarn, P
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit