• Purification and enrichment of intact saliva exosomes
  • Versatile sample input ranging from 0.5 mL to 2 mL
  • Process fresh saliva or saliva collected with Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400).
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Purify pure exosomes that are free from RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • RNA can be isolated from the purified exosomes using Norgen's Exosome RNA Purification kit (Cat. 58000)

ItemsCat. #Size
Saliva Exosome Purification Kit 65300 50 preps

Saliva Exosome Purification Kit

Exosomes are 40 - 150 nm membrane vesicles which are secreted by most cell types. Exosomes can be found in plasma, serum, saliva, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes and other extracellular vesicles (EVs) which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNA may serve as biomarkers for various diseases including cancer. Another subset of RNA that is found in saliva is the free-circulating RNA (fc-RNA). These fc-RNA are usually protein-bound RNA that are leaked from cells either during apoptosis or necrosis. As the RNA molecules encapsulated within exosomes or bound to proteins (fc-RNA) are protected from degradation by RNAses, they can be efficiently recovered from biological fluids such as saliva. In general, these two RNA groups contain valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status.

Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-bound proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25ºC) for up to 2 years without showing any reduction in performance.

Protocols: