Saliva Exosome Purification Kit For the purification and enrichment of exosomes from fresh or preserved saliva samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva Exosome Purification Kit For the purification and enrichment of exosomes from fresh or preserved saliva samples Price USD$819.00 SKU 65300 Format Spin Column Size 50 Preps Quantity Overview Details Documentation FAQs Citations Overview Versatile sample input ranging from 0.5 mL to 2 mL No time-consuming ultracentrifugation, filtration nor special syringes required No precipitation reagents or overnight incubation required No protease treatment required Purify pure exosomes that are free from RNA-binding proteins Analyze purified exosomes using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration Process fresh saliva or saliva collected with Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). Isolate high quality RNA from purified exosomes using Norgen's Exosome RNA Purification Kit (Cat. 58000) Purification is based on Norgen’s proprietary resin Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-bound proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression. Details Kit Specifications Saliva Input 500μL - 2mL Size of Exosome Purified 40nm - 150nm Elution Volume 200μL Time to Complete 10 Purifications 15 - 30 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat No. 65300 (50 preps) Slurry E 12.5 mL ExoC Buffer 1.5 mL ExoR Buffer 12 mL Mini Filter Spin Columns inserted into 2 mL tubes 50 Product Insert 1 Documentation PROTOCOLS (65300) Saliva Exosome Purification Kit - Protocol RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class FAQs Spin Column What should I do if some of the grey resin is transferred out when I am decanting the saliva supernatant? Simply remix and recentrifuge. After centrifuging decant the supernatant What if I added more or less of Slurry E? Adding less volume may reduce the amount of the purified exosomes. Adding more may not affect the exosome capture but may affect the release of the purified exosomes in the ExoR Buffer. What if I added more or less of ExoC Buffer? Adding a different volume from the specified optimum volume will significantly reduce the amount of the purified exosomes. What if I added more or less of ExoR Buffer? Adding less volume will reduce the release of the captured exosomes in the ExoR Buffer. Adding more will not affect the release of the captured exosomes but it will be more diluted. What will happen if accidently some of the grey resin was transferred with the ExoR buffer? Any grey resin will be filtered through the Mini Filter Spin Column and the flowthrough which contains the purified exosomes should not contain any grey resin. Is ExoR buffer from Norgen's exosome isolation kit compatible with proteomic downstream applications? ExoR buffer is compatible with most proteomic downstream applications. The ExoR buffer doesn't contain any detergents. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications. How to store the purified exosomes? If not using for downstream application immediately, it is recommended to store the purified exosomes at -80 degrees. Are exosomes purified using Norgen Biotek's technology biology active? Yes, the exosomes purified using Norgen Biotek's technology are biology active. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications. Citations Powered by Bioz See more details on Bioz Related Products Saliva Exosome Collection and Preservation Kit
Saliva Exosome Purification Kit For the purification and enrichment of exosomes from fresh or preserved saliva samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva Exosome Purification Kit For the purification and enrichment of exosomes from fresh or preserved saliva samples Price USD$819.00 SKU 65300 Format Spin Column Size 50 Preps Quantity
