Purification is based on Norgen’s proprietary resin
Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.
Details
Kit Specifications
Saliva Input
500μL - 2mL
Size of Exosome Purified
40nm - 150nm
Elution Volume
200μL
Time to Complete 10 Purifications
15 - 30 minutes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Adding less volume may reduce the amount of the purified exosomes. Adding more may
not affect the exosome capture but may affect the release of the purified exosomes in the
ExoR Buffer.
Adding less volume will reduce the release of the captured exosomes in the ExoR Buffer. Adding more will not affect the release of the captured exosomes but it will be more diluted.
Any grey resin will be filtered through the Mini Filter Spin Column and the flowthrough
which contains the purified exosomes should not contain any grey resin.
ExoR buffer is compatible with most proteomic downstream applications. The ExoR buffer doesn't contain any detergents. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications.
Yes, the exosomes purified using Norgen Biotek's technology are biology active. Please contact our Tech support team at support@norgenbiotek.com and ask for reference publications.
Adding less volume may reduce the amount of the purified exosomes. Adding more may not affect the exosome capture but may affect the release of the purified exosomes in the ExoR Buffer.
Adding less volume will reduce the release of the captured exosomes in the ExoR Buffer. Adding more will not affect the release of the captured exosomes but it will be more diluted.
Any grey resin will be filtered through the Mini Filter Spin Column and the flowthrough which contains the purified exosomes should not contain any grey resin.
The ExoR buffer doesn't contain any detergents, and is compatible with most of proteomic downstream applications. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.
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