Saliva/Swab RNA Purification Kits For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva/Swab RNA Purification Kits For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva Price USD$299.00 SKU 69100 Format Spin Column High Throughput Size 50 Preps Quantity Request sample Overview Details Automation Documentation Citations Overview For use on preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200) Fast and easy processing using rapid spin column format Available in a high throughput 96-well plate format for rapid purifications Isolate total RNA, from large rRNA down to microRNA (miRNA) No phenol or chloroform extractions Bind & elute all RNA irrespective of size or GC content, without bias Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services CE-IVD marked version available Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS. Saliva/Swab RNA Purification Kit (Spin Column) This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes. Saliva/Swab RNA Purification 96-Well Kit (High Throughput) High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen's NGS Services. Details Supporting Data Previous Figure 1. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Saliva samples were collected into Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800). All collected samples were spiked with 3 different concentrations from Norgen’s E/RdRP/RP Positive Control (included in Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP genes – TM67200) and processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification Kit (Cat. #69100), using an input of 0.25 mL from each preserved samples. The sensitivity of the isolation method was assessed by amplifying the spiked SARS-CoV-2 spiked targets using Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (TM67200). Figure 1 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgen’s Saliva/Swab RNA Purification Kit (Cat. #69100). Figure 2. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgen’s Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved sample or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR. Figure 3. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgen’s Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR. Figure 4. Average RNA Yield of Samples Isolated by Norgen’s Saliva/Swab RNA Purification Kit (Cat. #69100). The average yield of RNA isolated by Norgen’s Saliva/Swab RNA Purification Kit is ≥ 1 µg. Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat #69200) and preserved saliva samples were collected using Norgen’s Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA. Figure 5. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Saliva samples were collected into Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800). All collected samples were spiked with 3 different concentrations from Norgen’s E/RdRP/RP Positive Control (included in Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP genes – TM67200) and processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300), using an input of 0.25 mL from each preserved sample. The sensitivity of the isolation method was assessed by amplifying the spiked SARS-CoV-2 spiked targets using Norgen’s COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (TM67200). Figure 1 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgen’s Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Figure 6. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgen’s Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification 96-Well Kit, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR. Figure 7. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgen’s Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification 96-Well Kit, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR. Figure 8. Average RNA Yield of Samples Isolated by Norgen’s Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgen’s Total Nucleic Acid Preservative Tubes (Cat #69200) and preserved saliva samples were collected using Norgen’s Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgen’s Saliva/Swab RNA Purification 96-Well Kit, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA. Next Click for expanded view Kit Specifications Sample Volume Range 250 μL Size of RNA Purified All sizes, including small RNA (<200 nt) Minimum Elution Volume 50 μL Maximum Elution Volume 100 μL Time to Complete 10 Purifications 15 - 20 minutes Average Yield ≥ 1 μg * *Varies from sample to sample Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Kit ComponentCat. 69100 (50 preps)Cat. 69300 (192 preps)Lysis Buffer A30 mL100 mLSolution WN18 mL55 mLWash Solution A18 mL2 x 38 mLElution Solution A6 mL20 mLMini Spin Columns50-Collection Tubes50-Elution Tubes (1.7 mL)50-96-Well Isolation Plate-296-Well Collection Plate-296-Well Elution Plate-2Adhesive Tape-4Product Insert11 Automation Saliva/Swab Automated RNA Purification Tutorial Supplementary Protocol Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration Application Note Application Note - Comparing the Isolation Efficiency of Norgen’s Saliva/Swab RNA Purification Kit on Hamilton Vantage and QIAsymphony DSP Virus/Pathogen Kit Documentation PROTOCOLS PI69100-4 Saliva Swab RNA Purification Kit Insert.pdf (69300) Saliva/Swab RNA Purification 96-Well Kit (High Throughput) - Protocol (2 x 96-well plates) SUPPLEMENTARY PROTOCOLS Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration APPLICATION NOTES Comparison of Norgen’s Saliva/Swab RNA Purification Kit to a Magnetic Bead-Based Competitor's Kit for Viral RNA Extraction Comparison of Norgen’s Saliva/Swab RNA Purification Kit to Zymo’s MagBinding Quick-Viral DNA/RNA kit for Viral RNA Extraction Using Norgen’s Saliva/Swab RNA Purification Kit on the Hamilton Vantage to Isolate RNA from Fresh and Preserved Saliva Samples without Contamination RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Services NGS Related Products Saliva RNA Collection and Preservation Devices Saliva/Swab RNA Purification 96-Well Kit Total Nucleic Acid Preservation Tubes Total RNA Purification Kit
Saliva/Swab RNA Purification Kits For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva/Swab RNA Purification Kits For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva Price USD$299.00 SKU 69100 Format Spin Column High Throughput Size 50 Preps Quantity Request sample