Saliva/Swab RNA Purification Kits Dx

Cat. Dx69100, Dx69300

Saliva/Swab RNA Purification 96-Well Kit

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Saliva/Swab RNA Purification Kits Dx

Cat. Dx69100, Dx69300

For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva

For use from preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200)
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Fits into in vitro diagnostic workflows
Isolate high-quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples
Fast and easy processing using rapid spin column format
Isolate total RNA, from large rRNA down to microRNA (miRNA)
No phenol or chloroform extractions
Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA
Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

The purified RNA is intended for in vitro diagnostic use for medical purposes.

Research versions available here

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Saliva / Swab RNA Purification Kit Dx
(Cat. Dx69100) 50 Preps

Saliva/Swab RNA Purification 96-Well Kit Dx
(Cat. Dx69300) 2 x 96-Well Plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. Dx69100

Kit Specifications
Sample Volume Range	250 μL
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	15 - 20 minutes
Average Yield	≥ 1 μg * *Varies from sample to sample

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. Dx69300

Kit Specifications
Sample Volume Range	250 µL
Size of RNA purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	75 µL
Maximum Elution Volume	100 µL
Time to complete 96 purifications	30 minutes
Average Yield	≥ 1 µg * *varies from sample to sample

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels.

Supporting Data

Figure 1. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat. 69200). Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat. RU53800). All collected samples were spiked with 3 different concentrations from Norgens E/RdRP/RP Positive Control (included in Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes TM67200) and processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit (Cat. #69100), using an input of 0.25 mL from each preserved sample. The sensitivity of the isolation method was assessed by amplifying the spiked SARS-CoV-2 spiked targets using Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (TM67200). Figure 1 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgens Saliva/Swab RNA Purification Kit (Cat. #69100).

Figure 2. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #Dx69100). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes. Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices. Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit (Cat. #Dx69100), using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR.

Figure 3. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #Dx69100). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes. Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices. Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit (Cat. #Dx69100), using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR.

Figure 4. Average RNA Yield of Samples Isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #Dx69100). The average yield of RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #Dx69100) is ≥ 1 µg. Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgens Total Nucleic Acid Preservative Tubes (Lot #598129) and preserved saliva samples were collected using Norgens Saliva RNA Collection and Preservation Devices. Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA.

Figure 5. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. Dx69300). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat. Dx69200). Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat. 53800). All collected samples were spiked with 3 different concentrations from Norgens E/RdRP/RP Positive Control (included in Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes TM67200) and processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. Dx69300), using an input of 0.25 mL from each preserved sample. The sensitivity of the isolation method was assessed by amplifying the spiked SARS-CoV-2 spiked targets using Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (DxTM67200). Figure 5 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. Dx69300).

Figure 6. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit Dx (Cat. #Dx69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes Dx (Cat # Dx69300). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices Dx (53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit Dx, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR.

Figure 7. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit Dx (Cat. #Dx69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes Dx (Cat. #Dx69200). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices Dx (Cat # 53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit Dx, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR.

Figure 8. Average RNA Yield of Samples Isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit Dx (Cat. #Dx69300). Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgens Total Nucleic Acid Preservative Tubes Dx and preserved saliva samples were collected using Norgens Saliva RNA Collection and Preservation Devices (Cat. #53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit Dx, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA.

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Protocols:

(Dx69100) Saliva / Swab RNA Purification Kit Dx (Spin Column) - Protocol (50 preps)

(Dx69300) Saliva / Swab RNA Purification 96-Well Kit Dx (High Throughput) - Protocol (2 x 96-well plates)

Supplementary Protocols:

Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Notes:

Comparison of Norgen’s Saliva/Swab RNA Purification Kit to a Magnetic Bead-Based Competitor's Kit for Viral RNA Extraction

Comparison of Norgen’s Saliva/Swab RNA Purification Kit to Zymo’s MagBinding Quick-Viral DNA/RNA kit for Viral RNA Extraction

Supplementary Protocol

Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Note

Application Note - Comparing the Isolation Eff iciency of Norgen’s Saliva/Swab RNA Purif ication Kit on Hamilton Vantage and QIAsymphony DSP Virus/Pathogen Kit

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Saliva/Swab RNA Purification Kits Dx

These kits provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The chemistry employed in the kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitro diagnostic use for medical purposes.

Saliva/Swab RNA Purification Kit Dx (Spin Column)

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Saliva/Swab RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s Saliva/Swab RNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Saliva/Swab RNA Purification 96-Well Kit Dx (High Throughput)

This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

High throughput 96-well plate format for rapid purifications
Very consistent well-to-well RNA isolation
Bind & elute all RNA irrespective of size or GC content, without bias

Saliva RNA Extraction Using Norgen's Resin Technology