Saliva/Swab RNA Purification Kits

Cat. 69100, 69300

Saliva/Swab RNA Purification 96-Well Kit

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Saliva/Swab RNA Purification Kits

Cat. 69100, 69300

For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva

For use on preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200)
Fast and easy processing using rapid spin column format
Available in a high throughput 96-well plate format for rapid purifications
Isolate total RNA, from large rRNA down to microRNA (miRNA)
No phenol or chloroform extractions
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
CE-IVD marked version available
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Saliva/Swab RNA Purification Kit
(Cat. 69100) 50 Preps

Saliva/Swab RNA Purification 96-Well Kit
(Cat. 69300) 2 x 96-Well Plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

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Kit Specifications

You have selected: Cat. 69100

Kit Specifications
Sample Volume Range	250 μL
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	15 - 20 minutes
Average Yield	≥ 1 μg * *Varies from sample to sample

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 69300

Kit Specifications
Sample Volume Range	250 µL
Size of RNA purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	75 µL
Maximum Elution Volume	100 µL
Time to complete 96 purifications	30 minutes
Average Yield	≥ 1 µg * *varies from sample to sample

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Figure 1. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat. 69200). Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat. RU53800). All collected samples were spiked with 3 different concentrations from Norgens E/RdRP/RP Positive Control (included in Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes TM67200) and processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit (Cat. #69100), using an input of 0.25 mL from each preserved samples. The sensitivity of the isolation method was assessed by amplifying the spiked SARS-CoV-2 spiked targets using Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (TM67200). Figure 1 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgens Saliva/Swab RNA Purification Kit (Cat. #69100).

Figure 2. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved sample or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR.

Figure 3. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #69100). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR.

Figure 4. Average RNA Yield of Samples Isolated by Norgens Saliva/Swab RNA Purification Kit (Cat. #69100). The average yield of RNA isolated by Norgens Saliva/Swab RNA Purification Kit is ≥ 1 µg. Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200) and preserved saliva samples were collected using Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA.

Figure 5. SARS-CoV-2 E-gene and RdRP Amplification from Preserved Swabs and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat. 69200). Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat. RU53800). All collected samples were spiked with 3 different concentrations from Norgens E/RdRP/RP Positive Control (included in Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes TM67200) and processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300), using an input of 0.25 mL from each preserved sample. The sensitivity of the isolation method was assessed by amplifying the spiked SARS-CoV-2 spiked targets using Norgens COVID-19 TaqMan RT-PCR Kit (E/RdRP genes (TM67200). Figure 1 shows the linear recovery of the different spike-in amounts indicating the high sensitivity of the isolation procedure as well as the high quality of the RNA purified using Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300).

Figure 6. RNase P Gene Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the RNase P gene by RT-PCR.

Figure 7. Human miR-21 Amplification of Fresh and Preserved Swab and Saliva RNA isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate preserved (P) and non-preserved (NP) Nasopharyngeal swabs, Oropharyngeal Swabs, and Saliva samples were collected from different donors. Preserved Nasopharyngeal and Oropharyngeal swab samples were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200). Preserved Saliva samples were collected into Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit, using an input of 0.25 mL from preserved samples or the entire non-preserved swab for the non-preserved conditions. Non-preserved saliva samples were isolated from a 0.25mL input as well. Isolated RNA was tested by amplification of the human miR-21 detected by stem-loop RT-PCR.

Figure 8. Average RNA Yield of Samples Isolated by Norgens Saliva/Swab RNA Purification 96-Well Kit (Cat. #69300). Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200) and preserved saliva samples were collected using Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification 96-Well Kit, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA.

Click for expanded view

Protocols:

(69100) Saliva/Swab RNA Purification Kit (Spin Column) - Protocol (50 preps)

(69300) Saliva/Swab RNA Purification 96-Well Kit (High Throughput) - Protocol (2 x 96-well plates)

Supplementary Protocols:

Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Notes:

Comparison of Norgen’s Saliva/Swab RNA Purification Kit to a Magnetic Bead-Based Competitor's Kit for Viral RNA Extraction

Comparison of Norgen’s Saliva/Swab RNA Purification Kit to Zymo’s MagBinding Quick-Viral DNA/RNA kit for Viral RNA Extraction

Using Norgen’s Saliva/Swab RNA Purification Kit on the Hamilton Vantage to Isolate RNA from Fresh and Preserved Saliva Samples without Contamination

Saliva/Swab Automated RNA Purification Tutorial

Supplementary Protocol

Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Note

Application Note - Comparing the Isolation Eff iciency of Norgen’s Saliva/Swab RNA Purif ication Kit on Hamilton Vantage and QIAsymphony DSP Virus/Pathogen Kit

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Saliva/Swab RNA Purification Kits

Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.

Saliva/Swab RNA Purification Kit (Spin Column)

This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.

Saliva/Swab RNA Purification 96-Well Kit (High Throughput)

High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen's NGS Services.

Saliva RNA Extraction Using Norgen's Resin Technology

Saliva/Swab RNA Purification Kit Tutorial (Cat. 69100)

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 69100

Kit Specifications
Sample Volume Range	250 μL
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	15 - 20 minutes
Average Yield	≥ 1 μg * *Varies from sample to sample

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 69300

Kit Specifications
Sample Volume Range	250 µL
Size of RNA purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	75 µL
Maximum Elution Volume	100 µL
Time to complete 96 purifications	30 minutes
Average Yield	≥ 1 µg * *varies from sample to sample

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Protocols:

(69100) Saliva/Swab RNA Purification Kit (Spin Column) - Protocol (50 preps)

(69300) Saliva/Swab RNA Purification 96-Well Kit (High Throughput) - Protocol (2 x 96-well plates)

Supplementary Protocols:

Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Notes:

Comparison of Norgen’s Saliva/Swab RNA Purification Kit to a Magnetic Bead-Based Competitor's Kit for Viral RNA Extraction

Comparison of Norgen’s Saliva/Swab RNA Purification Kit to Zymo’s MagBinding Quick-Viral DNA/RNA kit for Viral RNA Extraction

Using Norgen’s Saliva/Swab RNA Purification Kit on the Hamilton Vantage to Isolate RNA from Fresh and Preserved Saliva Samples without Contamination

Saliva/Swab Automated RNA Purification Tutorial

Supplementary Protocol

Supplementary Protocol - Automated Procedure for Saliva/Swab RNA Purification and Concentration