Salmonella enterica TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Salmonella enterica TaqMan PCR Detection Kit Dx SKU DxTM32100 Format PCR Size 24 Reactions Contact Distributor Overview Details Documentation Citations Overview Detection kits for Salmonella enterica CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in TaqMan Probe format for analysis Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer. TaqMan PCR Kit Click to expand options Salmonella enterica TaqMan PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions The Salmonella enterica TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM32100 (24 rxns) MDx TaqMan 2X PCR Master Mix Dx 550 µL S. enterica Primer & Probe Mix Dx 2 x 70 µL S. enterica Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM32100) Salmonella enterica TaqMan PCR Kit Dx - Protocol (24 rxns) Related Products Milk Bacterial DNA Isolation Kit Bacterial Genomic DNA Isolation Kit
Salmonella enterica TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Salmonella enterica TaqMan PCR Detection Kit Dx SKU DxTM32100 Format PCR Size 24 Reactions Contact Distributor
