• Detection kits for Salmonella enterica
  • Available in 3 formats for analysis:
 

ItemsCat. #Size
Salmonella enterica TaqMan PCR Kit TM32100 24 rxns
Salmonella enterica TaqMan Probe/Primer and Control Set TM32110 100 rxns
Salmonella enterica SYBR Green PCR Kit SG32100 24 rxns
Salmonella enterica End-Point PCR Kit EP32100 24 rxns
Salmonella enterica Primer and Control Set EP32110 100 rxns

Salmonella enterica Detection Kits

Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer.

There are 3 kits available for the detection of Salmonella enterica:

Salmonella enterica TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
Salmonella enterica SYBR Green PCR Kit
  • Master Mix for the target detection
  • Control Master Mix for the PCR validation
  • Primers for the specific amplification of a 336 bp region of the S. enterica genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
Salmonella enterica End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a 336 bp region of the S. enterica genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM32100
(24 rxns)
SYBR Green
Cat. SG32100
(24 rxns)
End-Point
Cat. EP32100
(24 rxns)
MDx 2X PCR Master Mix - - 350 µL
MDx SYBR 2X PCR Master Mix - 350 µL -
MDx SYBR 2X PCR Control Master Mix - 350 µL -
MDx TaqMan 2X PCR Master Mix 350 µL - -
Salmonella enterica Primer Mix 70 µL 70 µL 70 µL
Salmonella enterica Positive Control 50 µL 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL 1.25 mL
Loading Dye - - 100 µL
DNA Ladder - - 100 µL
Product Insert 1 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM32110
(100 rxns)
Primer
and Control Set
Cat. EP32110
(100 rxns)
Salmonella enterica Primer Mix 3 x 70 µL 3 x 70 µL
Salmonella enterica Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1