For the rapid preparation of inhibitor-free total RNA from soil
|Soil RNA Purification Kit||27750||50 preps|
Soil Total RNA Purification Kit
This kit provides a convenient and rapid spin column method to purify total RNA from small amounts of soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acids and other inhibitors of PCR using humic acid removal (HAR) columns and the organic substance removal solution (OSR).
Norgen’s Soil Total RNA Purification Kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA without the need for phenol. The purified RNA is of the highest integrity and is inhibitor-free for any downstream application including microbiome studies, real time PCR and reverse transcription PCR for gene expression analysis, Next Generation Sequencing and more.
Suggested Soil Input
(Clay, loam, sand, feces, compost)
|Type of Soil Processed||All types, including common soil,
compost and manure
|Maximum Column Binding Capacity||50 μg|
|Maximum Column Loading Volume||650 μL|
|Time to Complete Purifications||30 minutes|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Soil CO2 emission partitioning, bacterial community profile and gene expression of Nitrosomonas spp. and Nitrobacter spp. of a sandy soil amended with biochar and compost|
|Journal||Applied Soil Ecology. 2017.|
|Authors||Giovambattista Sorrenti, Giampaolo Buriani, Francesca Gaggìa, Loredana Baffoni, Francesco Spinelli, Diana Di Gioia, Moreno Toselli|
|Title||Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).|
|Journal||Microbiota and Metabolome of IgA Nephropathy Patients. 2014.|
|Authors||Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.|
|Title||Extraction of Bacterial RNA from Soil: Challenges and Solutions.|
|Journal||Microbes and the Environment / JSME. 2012.|
|Authors||Wang Y, Hayatsu M, Fujii T.|
|Title||Detection of viable Cryptosporidium parvum in soil by reverse transcription real time PCR targeting hsp70 mRNA.|
|Journal||Applied and Environmental Biology. 2011.|
|Authors||Liang Z, Keeley A.|
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