Soil Total RNA Purification Kit For the rapid preparation of inhibitor-free total RNA from soil For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil Total RNA Purification Kit For the rapid preparation of inhibitor-free total RNA from soil Price USD$515.00 SKU 27750 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Isolate high quality total RNA from a variety of soil samples Process all types of soil, including common soil, compost and manure Remove all traces of humic acids and other inhibitors of PCR Isolates all sizes of RNA, including microRNA, without phenol Complete kit including bead tubes and humic acid removal columns (HAR) Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a convenient and rapid spin column method to purify total RNA from small amounts of soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acids and other inhibitors of PCR using humic acid removal (HAR) columns and the organic substance removal solution (OSR). Norgen’s Soil Total RNA Purification Kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA without the need for phenol. The purified RNA is of the highest integrity and is inhibitor-free for any downstream application including microbiome studies, real time PCR and reverse transcription PCR for gene expression analysis, Next Generation Sequencing and more. Details Supporting Data Previous Figure 1. Isolation of Total RNA from Bacteria in Soil. Pseudomonas fluorescens was spiked into 250 mg samples of autoclaved soil and total RNA was isolated using Norgen's Soil Total RNA Purification Kit. RNA was visualized by running 7.5 µL of each 75 µL elution on a 1.2% agarose-formaldehyde RNA gel. Total RNA (large and small) of Pseudomonas fluorescens was recovered from the autoclaved spiked soil without any significant degradation, indicating that high integrity RNA can be purified from the microorganisms in the soil. Lanes 1 and 2 contain total RNA from Pseudomonas fluorescens, Lanes 3 and 4 contain total RNA purified from the autoclaved soil spiked with Pseudomonas fluorescens, and Lanes 5 and 6 contain RNA purified from the autoclaved soil (no RNA was found). Figure 2. High Quality RNA Free from PCR Inhibitors. Pseudomonas fluorescens was spiked into 250 mg samples of autoclaved soil and total RNA was isolated using Norgen's Soil Total RNA Purification Kit. One microliter of each elution was then used as the template in a 20 µL RT-PCR reaction to detect the 16s rRNA. Lane 1 contains the results when total RNA from Pseudomonas fluorescens was used as the input, Lane 2 is the results when total RNA isolated from the autoclaved soil spiked with Pseudomonas fluorescens was used as the input, Lane 3 is the results when RNA purified from non-spiked autoclaved soil was the input, Lane 4 is a positive control and Lane 5 is the Negative control. As it can be seen the RNA purified from soil using Norgen's kit was of a high quality and can be successfully used in sensitive downstream applications. Next Click for expanded view Kit Specifications Suggested Soil Input (Clay, loam, sand, feces, compost) 500 mg Type of Soil Processed All types, including common soil, compost and manure Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Time to Complete Purifications 30 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 27750 (50 preps) Lysis Buffer I 2 x 20 mL Binding Buffer E 6 mL Solution BX 9 mL Lysis Buffer QP 25 mL Binding Buffer B 30 mL Wash Solution A 18 mL Elution Solution A 6 mL Bead Tubes 50 Spin Columns 50 Humic Acid Removal Columns 50 Collection Tubes 100 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (27750) Soil Total RNA Purification Kit - Protocol (50 prep) SUPPLEMENTARY PROTOCOLS Soil RNA Purification Kit - Supplementary Protocol for Isolation of RNA from Waste Water APPLICATION NOTES Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve SAFETY DATA SHEETS (27750) Soil Total RNA Purification Kit - Safety Data Sheet POSTERS Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Wastewater Surveillance: Detecting Disease Outbreaks Earlier Citations Title Monitoring SARS-CoV-2 variants in wastewater of Dhaka City, Bangladesh: approach to complement public health surveillance systems Journal Human Genomics. 2023. Authors Haque, Rehnuma, et al. Title Soil CO2 emission partitioning, bacterial community profile and gene expression of Nitrosomonas spp. and Nitrobacter spp. of a sandy soil amended with biochar and compost Journal Applied Soil Ecology. 2017. Authors Giovambattista Sorrenti, Giampaolo Buriani, Francesca Gaggìa, Loredana Baffoni, Francesco Spinelli, Diana Di Gioia, Moreno Toselli Title Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN). Journal Microbiota and Metabolome of IgA Nephropathy Patients. 2014. Authors Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo. Title Extraction of Bacterial RNA from Soil: Challenges and Solutions. Journal Microbes and the Environment / JSME. 2012. Authors Wang Y, Hayatsu M, Fujii T. Title Detection of viable Cryptosporidium parvum in soil by reverse transcription real time PCR targeting hsp70 mRNA. Journal Applied and Environmental Biology. 2011. Authors Liang Z, Keeley A. Related Products RNase-Free DNase I Kit Water RNA/DNA Purification Kit - 0.45 µm
Soil Total RNA Purification Kit For the rapid preparation of inhibitor-free total RNA from soil For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil Total RNA Purification Kit For the rapid preparation of inhibitor-free total RNA from soil Price USD$515.00 SKU 27750 Format Spin Column Size 50 Preps Quantity
