Total RNA Purification Kits Dx

Cat. Dx17200, Dx24300, Dx24350, Dx24380

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What Scientists are Saying About this Product

Our RNA isolations with your test kit look very promising...We obtained large quantities of intact RNA with a 260/280 of 1.99...The other thing I should mention about the RNA kit is that we saw no DNA - which is great!

Donovan Bailey

The Walter and Eliza Hall Institute of Medical Research

We have done a comparison between your (Norgen's) Total RNA Purification kit and [the competitor] kit. The RNA was extracted from two identical samples of cultured cells (~500 000 cells per sample). As you can tell there's hardly any difference between the two kits in terms of RNA quality or quantity of small RNA species. However, Norgen kit costs half the price of the [competitor] one, takes a lot less time to run through the protocol and doesn't require additional reagents (such as Trizol). All of which makes a strong case in favour of using the Norgen kit.

The Walter and Eliza Hall Institute of Medical Research

Fred Hutchison Cancer Research Center, USA

I did try the Norgen RNA Kit and it went very well. I isolated RNA from cultured cells. I then compared it to our standard [competitor] isolation method and the Norgen kit gave more purified RNA than [competitor].

Fred Hutchison Cancer Research Center, USA

I did RNA isolation using different kits and detection afterwards microRNAs by q-PCR. Your total RNA isolation kit works better than the rest, at least in my hands.

McMaster University

…the All-in-One purification kit that [I] brought. From the same cell samples, we tested the miRNAs and the proteins and they seem to be happy with their results. In terms of miRNA purification,the total RNA extraction kit seems also to do better than other companies.

McMaster University

I just used a [competitor's] spin column to isolate some RNA and the yields were acceptable, but considerably less than what I'm used to (about 8x less). This leaves me with 2 poor yields out of 5 attempts with these columns. I was willing to chalk the first poor yield to "my error" (losing tissue or some other fluke event), but now I'm worried that the [competitor] columns are unreliable - good when they work but prone to failing from time to time... I've never had a poor yield in any of the 8 attempts I've tried with the Norgen columns.

St. Michael's Hospital

Total RNA Purification Kits Dx

Cat. Dx17200, Dx24300, Dx24350, Dx24380

For the isolation and purification of total RNA from tissue samples, blood, plasma, serum, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Available for sale in Canada (Dx17200, Dx24300)
Ideal for use in in vitro diagnostic workflows
Extract high quality & quantity total RNA including miRNA
No phenol step required
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Note: This product is not available for sale in the United States

Ideal for in vitro diagnostics use.

Research versions available here

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Total RNA Purification Kit Dx
(Cat. Dx17200) 50 preps

Total RNA Purification 96-Well Kit Dx
(Cat. Dx24300) 2 x 96-well plates

Total RNA Purification Kit - 96 Deep Well Plate Format Dx
(Cat. Dx24350) 2 x 96-well plates

Total RNA Purification Kit - 96 Deep Well Plate Format Dx
(Cat. Dx24380) 6 x 96-well plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. Dx17200

Kit Specifications
Binding Capacity Per Column	Up to 50 μg RNA
Maximum Loading Volume Per Spin Column	650 μL
Size of RNA Purified	All sizes, including < 200 nt
Time to Complete 10 Purifications	20 minutes
Maximum Amount of Starting Material
Tissues	10 mg (for most tissues)
Blood	100 µL
Plasma/Serum	200 µL
Bacteria	1 x 10⁹cells
Yeast	1 x 10⁸ cells
Fungi	50 mg

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels

You have selected: Cat. Dx24300

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 10⁶ Cells) E. coli (1 x 10⁹ cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL 150 µL 1 x 10⁹ cells 1 x 10⁸cells 40 mg 40 mg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. Dx24350

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 10⁶ Cells) E. coli (1 x 10⁹ cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL 150 µL 1 x 10⁹ cells 1 x 10⁸cells 40 mg 40 mg

You have selected: Cat. Dx24380

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 10⁶ Cells) E. coli (1 x 10⁹ cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL 150 µL 1 x 10⁹ cells 1 x 10⁸cells 40 mg 40 mg

Supporting Data

Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit Dx allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 10⁹ E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.

Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction. Norgen's Total RNA Purification Kit Dx isolates the complete size range of RNA, including small RNAs without the use of phenol. Total RNA was isolated from 0.75 million HeLa cells using Norgen's Total RNA Purification Kit Dx, various silica-based competitors and phenol-based TRI Reagent. RT-PCR was performed according to Shi and Chiang (2005). Fifteen microliters of the 50 µL isolated RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was used in a 20 µL qPCR reaction with primers against the human microRNAs (miR-19 and miR-21) and large mRNA (S15). Only the use of Norgen's Total RNA Purification Kit Dx resulted in detection of both microRNAs and mRNA amplification products similar to Competitor 2's kit but without the use of phenol.

Figure 3. High Quality of RNA from a Diverse Range of Inputs. Norgen's Total RNA Purification Kit Dx allows RNA isolation from a wide range of species or tissue types. Total RNA was isolated from 5 x 10⁸ E. coli cells, 1 x 10⁶ HeLa cells, 100 µL rat blood and 10 mg hamster kidney using Norgen's Total RNA Purification Kit Dx. One microliter of the 50 µL isolated RNA was analyzed on the Agilent® 2100 BioAnalyzer using an RNA Nano 6000 chip. Note the integrity of RNA from all inputs with the presence of small RNA species. Norgen's Total RNA Purification Kit Dx consistently isolates high quality RNA from various inputs that score a RIN value between 8 and 10.

Figure 4. Great Isolation Sensitivity. Norgen's Total RNA Purification Kit Dx allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of 293 HEK cells from 1 million cells down to a single cell. Five microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL PCR reaction with primers to detect the human beta-actin transcripts. PCR products of beta-actin were detected from as little as a single cell. M is the marker lane.

Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA. Norgens Total RNA Purification Kit Dx allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit Dx (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification Dx had the best linearity (higher R²) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21).

Figure 6. Effective and Consistent Detection of miRNA from Plasma. Norgen's Total RNA Purification Kit Dx can effectively isolate miRNA from plasma. Total RNA was isolated from 50, 100 or 200 µL of rat plasma in triplicates using Norgen's Total RNA Purification Kit Dx (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Stem loop RT-qPCR using primers specific to miR-21 was performed. In brief, two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21. Norgen's Total RNA Purification Kit Dx is the only product that showed (1) consistent detection of miR-21 transcripts across all input volumes, and (2) Ct values correlated to input volume (decrease Ct with increase input).

Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency. Norgen's Total RNA Purification Kit Dx isolates plasma RNA that performs more consistently in downstream applications such as microarrays. Total RNA including miRNA was isolated from 100 µL of mouse plasma in duplicate using Norgen's Total RNA Purification Kit Dx, Competitor 1's leading miRNA Kit or a phenol-based RNA extraction method. One hundred nanograms of extracted RNA from each kit was applied to an Illumina microRNA expression profiling kit. Scatter plots display better consistency (better clustering) of replicate signals from Norgen's samples. Genes with Pval < 0.01 for both replicates were in blue. The associated table suggested that Norgen's protocol recovered the same diversity of miRNA with better consistency (higher r² value).

Figure 8. Better Diversity of miRNA Detected from Plasma. Norgen's Total RNA Purification Kit Dx isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit Dx or 625 µL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit Dx (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitors miRNA kit (right). Image courtesy of LC Sciences, Houston.

Figure 9. Better Diversity of miRNA Detected from HeLa Cells using Illumina Small RNA Next Gen Sequencing.Norgen's Total RNA Purification Kit Dx isolates miRNA from HeLa cells with better diversity than a leading competitor. Total RNA including miRNA was isolated from 1 million HeLa cells using Norgen's Total RNA Purification Kit Dx and Competitor Q's leading miRNA Kit, and was applied to Illumina Small RNA Next Gen Sequencing on a MiSeq sequencer. Panel A showed that Norgens Total RNA Purification Kit Dx recovers ahigher number of miRNAsthan the competitor. In particular, the higher diversity is achieved with a faster and simpler procedure in as little as 20 minutes of RNA sample preparation time without the use of phenol. Panel B is a scatter plot of the average RPM (reads per million) of the miRNAs detected used to compare Norgen and the competitorskit recovery of miRNA. Norgens Total RNA Purification Kit Dx recovered a significantly higher number of miRNAs that have higher RPM, as summarized in the graph insert as well as in Panel C.

Figure 10. Consistent Yield of High Quality RNA with Complete Size Range without the Use of Phenol. Norgen's Total RNA Purification 96-Well Kit allows for the consistent isolation of high quality RNA, with complete sizes ranging from very large RNA down to small RNA without the use of phenol. Total RNA was isolated from 0.5 mL aliquots of an overnight culture of E. coli (~5 x 10⁸ cells/mL) using Norgen's Total RNA Purification 96-Well Kit in 16 replicates. Five microliters of the 75 µL isolated RNA were resolved on a 1.2% formaldehyde agarose gel. All 16 replicates showed both high yield and high quality. In addition, all replicates showed effective recovery of all sizes of RNA including the small RNA (arrow).

Figure 11. High Quality of RNA from a Diverse Range of Inputs. Norgen's Total RNA Purification 96-Well Kit allows for RNA isolation from a wide range of species and tissue types. Total RNA was isolated from 8 mg of brain (Lanes A), kidney (Lanes B), liver (Lanes C), lung (Lanes D) and spleen tissue (Lanes E), 7.5 x 10⁵ HeLa (Lanes F) and CHO cells (Lanes G), and 5 x 10⁸ bacteria (Lanes H) using Norgen's Total RNA Purification 96-Well Kit. Five microliters of the 75 µL isolated RNA was resolved on a 1.2% formaldehyde agarose gel. From observing the formaldehyde-agarose gel, it can be seen that Norgen's kit can be used to successfully isolate total RNA, including small RNA species, from a broad range of sample types.

Figure 12. Isolation of Both Large and Small RNA. Norgen's Total RNA Purification 96-Well Kit allows for consistent isolation of both large and small RNA. Total RNA was isolated from samples of 5 x 10⁵ HeLa cells using Norgen's Total RNA Purification 96-Well Kit. Aliquots of each total RNA sample were then used in 2 different RT-qPCR reactions. Ten microliters of the 75 µL isolated RNA was subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15 transcripts (Panel B), respectively. Both the mRNA and microRNA were amplified in a consistent manner from all the samples, with low variability of the Ct values. Thus both types of RNA are being consistently isolated using this kit.

Figure 13. Great Isolation Sensitivity. Norgen's Total RNA Purification Kit allows for sensitive RNA extraction from even less than 10 cells. RT-qPCR was used to detect mRNA isolated from various input amounts of HeLa cells, from 100,000 down to a single cell, using Norgen's Total RNA Purification 96-Well Kit. Ten microliters of the 75 µL isolated RNA were then subjected to a 20 µL reverse transcription using oligo dT primers. Three microliters of the reverse transcription were used in a 20 µL real-time PCR reaction with primers to detect the human S15 transcripts. The resulting Ct values were plotted against the input cell number. Total RNA was isolated and detected linearly from as little as a single HeLa cell.

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Protocols:

(Dx17200) Total RNA Purification Kit Dx (Spin Column) - Protocol (50 preps)

(Dx24300) Total RNA Purification 96-Well Kit Dx (High Throughput) - Protocol ( 2 x 96-well plates)

(Dx24350) Total RNA Purification 96-Well Kit Dx (High Throughput Deep Well) - Protocol (2 x 96-well plates)

(Dx24380) Total RNA Purification 96-Well Kit Dx (High Throughput Deep Well) - Protocol (6 x 96-well plates)

Supplementary Protocols:

Supplementary Protocol - Total RNA Isolation From Cerebrospinal Fluid Csf

Supplementary Protocol - Norgen's Saliva RNA Collection and Preservation Devices

Supplementary Protocol for Isolating Viral RNA from Swab Samples

Application Notes:

High-Throughput Purification of Total RNA Including miRNA without the use of Phenol Extraction

Isolation and Detection of RNA from as little as a Single Animal Cell Using Norgen’s Total RNA Purification Kit

Safety Data Sheets:

(24300) Total RNA Purification 96-Well Kit - Safety Data Sheet

Spin Column (Dx17200)

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Journal	Journal of Thoracic Disease. 2016.
Authors	Yang Liu, Xianwen Chen, Qian Bian, Yuan Shi, Qingdong Liu, Lu Ding, Hengdong Zhang, Baoli Zhu

Title	Effects of Megaplasmid Loss on Growth of Neurotoxigenic Clostridium butyricum Strains and Botulinum Neurotoxin Type E Expression
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Title	Tribulus terrestris Alters the Expression of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 in Rabbit Ovaries of Mothers and F1 Female Offspring
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Title	The Transcription Factor NFIL3 Is Essential for Normal Placental and Embryonic Development but not for Uterine Natural Killer (UNK) Cell Differentiation in Mice
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Authors	Redhead, M. L., Portilho, N. A., Felker, A. M., Mohammad, S., Mara, D. L., & Croy, B. A

Title	Cyclophilin A modulates bone marrow-derived CD117+ cells and enhances ischemia-induced angiogenesis via the SDF-1/CXCR4 axis
Journal	International Journal of Cardiology. 2016.
Authors	Perrucci GL, Straino S, Corlianò M, Scopece A, Napolitano M, Berk BC, Lombardi F, Pompilio G, Capogrossi MC, Nigro P

Title	Temporal embryonic transcription of chicken fast skeletal myosin heavy chain isoforms in the single comb white leghorn
Journal	Poultry Science. 2016.
Authors	Griffin J, St-Pierre N, Lilburn MS, Wick M

Title	PKLR promotes colorectal cancer liver colonization through induction of glutathione synthesis
Journal	The Journal of Clinical Investigation. 2016.
Authors	Nguyen A, Loo JM, Mital R, Weinberg EM, Man FY, Zeng Z, Paty PB, Saltz L, Janjigian YY, de Stanchina E, Tavazoie SF

Title	Identification of the genes involved in odorant reception and detection in the palm weevil Rhynchophorus ferrugineus, an important quarantine pest, by antennal transcriptome analysis
Journal	BMC Genomics. 2016.
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Title	Multifactorial Competition and Resistance in a Two-Species Bacterial System
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Authors	Anupama Khare, Saeed Tavazoie

Title	Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974.
Journal	Laboratory Investigation. 2015.
Authors	Boone JD, Arend RC, Johnston BE, Cooper SJ, Gilchrist SA, Oelschlager DK, Grizzle WE, McGwin G Jr, Gangrade A, Straughn JM Jr, Buchsbaum DJ

Title	Identification of a novel mechanism of action of fingolimod (FTY720) on human effector T cell function through TCF-1 upregulation
Journal	Journal of Neuroinflammation. 2015.
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Title	Effects of BRCA1 Knockdown on CYP19a1/Aromatase and Steroid Receptor Expression in Ovarian and Tubal Cells
Journal	Molecular and Genetic Medicine. 2015.
Authors	Jean S Fleming, Igor Ruza, Bryony A Thompson, Kai-Fai Lee, and Adele G Woolley

Title	PIK3R1 targeting by miR-21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT, and predicts clinical outcome of breast cancer
Journal	International Journal of Oncology. 2015.
Authors	Yan LX, Liu YH, Xiang JW, Wu QN, Xu LB, Luo XL, Zhu XL, Liu C, Xu FP, Luo DL, Mei P, Xu J, Zhang KP, Chen J

Title	PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P₂-dependent actin remodeling
Journal	The EMBO Journal. 2015.
Authors	Caitlin A Sengelaub, Kristina Navrazhina, Jason B Ross, Nils Halberg & Sohail F Tavazoie

Title	Placental growth factor deficiency is associated with impaired cerebral vascular development in mice
Journal	Molecular Human Reproduction. 2015.
Authors	Luna RL, Kay VR, Rätsep MT, Khalaj K, Bidarimath M, Peterson N, Carmeliet P, Jin A, Anne Croy B

Title	Systemic genome screening identified the outcome associated focal loss of long noncoding RNA PRAL in hepatocellular carcinoma.
Journal	Hepatology. 2015.
Authors	Zhou CC, Yang F, Yuan SX, Ma JZ, Liu F, Yuan JH, Bi FR, Lin KY, Yin JH, Cao GW, Zhou WP, Wang F, Sun SH

Title	Overexpression of quinone reductase from Salix matsudana Koidz enhances salt tolerance in transgenic Arabidopsis thaliana.
Journal	Gene. 2015.
Authors	Song X, Fang J, Han X, He X, Liu M, Hu J, Zhuo R

Title	Deep sequencing of mRNA in CD24⁻ and CD24⁺ mammary carcinoma Mvt1 cell line
Journal	Genome Data. 2015.
Authors	Ran Rostoker, Anitha D. Jayaprakash, Ravi Sachidananda, and Derek LeRoith

Title	Differential Role of G Protein-Coupled Receptor Kinase 5 in Physiological Versus Pathological Cardiac Hypertrophy
Journal	Circulation Research. 2015.
Authors	Christopher J Traynham, Alessandro Cannavo, Yan Zhou, Alexandre Vouga, Benjamin P Woodall, Jonathan E Hullmann, Jessica Ibetti, Jessica I Gold1, J Kurt Chuprun, Erhe Gao1 and Walter J Koch

Title	The pivotal role of high glucose-induced overexpression of PKCβ in the appearance of glucagon-like peptide-1 resistance in endothelial cells.
Journal	Endocrine. 2015.
Authors	Pujadas G, De Nigris V, La Sala L, Testa R, Genovese S, Ceriello A.

Title	Targeting the MDM2/MDM4 Interaction Interface as a Promising Approach for p53 Reactivation Therapy
Journal	Cancer Research. 2015.
Authors	Pellegrino M, Mancini F, Lucà R, Coletti A, Giacchè N, Manni I, Arisi I, Florenzano F, Teveroni E, Buttarelli M, Fici L, Brandi R, Bruno T, Fanciulli M, D'Onofrio M, Piaggio G, Pellicciari R, Pontecorvi A, Marine JC, Macchiarulo A, Moretti F

Title	Characterizing the physiological and behavioural roles of proctolin in Drosophila melanogaster
Journal	Journal of Neurophysiology. 2015.
Authors	Ormerod KG, LePine OK, Bhutta MS, Jung J, Tattersall GJ, Mercier AJ

Title	Δ9-Tetrahydrocannabinol (Δ9-THC) Promotes Neuroimmune-Modulatory MicroRNA Profile in Striatum of Simian Immunodeficiency Virus (SIV)-Infected Macaques
Journal	Journal of Neuroimmune Pharmacology. 2015.
Authors	Simon L, Song K, Vande Stouwe C, Hollenbach A, Amedee A, Mohan M, Winsauer P, Molina P

Title	RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold
Journal	Cell Reports. 2015.
Authors	Ann-Britt Marcher, Anne Loft, Ronni Nielsen, Terhi Vihervaara, Jesper Grud Skat Madsen, Marko Sysi-Aho, Kim Ekroos, Susanne Mandrup

Title	The Human Antibody Fragment DIATHIS1 Specific for CEACAM1 Enhances Natural Killer Cell Cytotoxicity Against Melanoma Cell Lines In Vitro
Journal	Journal of Immunotherapy. 2015.
Authors	Dupuis ML, Fiori V, Soriani A, Ricci B, Dominici S, Moricoli D, Ascione A, Santoni A, Magnani M, Cianfriglia M.

Title	Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917
Journal	FEMS Microbiology Letters. 2015.
Authors	Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW

Title	Quaking and miR-155 interactions in inflammation and leukemogenesis
Journal	Oncotarget. 2015.
Authors	Tili E, Chiabai M, Palmieri D, Brown M, Cui R, Fernandes C, Richmond T, Kim T, Sheetz T, Sun HL, Lagana A, Veneziano D, Volinia S, Rassenti L, Kipps T, Awad H, Michaille JJ, Croce CM

Title	Impact of δ-Tocotrienol on Inflammatory Biomarkers and Oxidative Stress in Hypercholesterolemic Subjects
Journal	Journal of Clinical & Experimental Cardiology. 2015.
Authors	Asaf A Qureshi, Dilshad A Khan, Wajiha Mahjabeen, Anne M Trias, Neerupama Silswal and Nilofer Qureshi

Title	Circulating miR-33a and miR-33b are up-regulated in familial hypercholesterolaemia in paediatric age
Journal	Clinical Science. 2015.
Authors	Martino F, Carlomosti F, Avitabile D, Persico L, Picozza M, Barillà F, Arca M, Montali A, Martino E, Zanoni C, Parrotto S, Magenta A.

Title	Cigarette Smoke Exposure Triggers the Autophagic Cascade via Activation of the AMPK Pathway in Mice
Journal	Biology of Reproduction. 2015.
Authors	Hayley C. Furlong, Martin R. Stämpfli, Anne M. Gannon, and Warren G. Foster

Title	Comparative Evaluation of NS-5 Mixture and its Components on Superoxide Production in HUVEC, and Inflammatory Biomarkers in Humans
Journal	Clinical & Experimental Cardiology. 2015.
Authors	Asaf A Qureshi, Dilshad A Khan, Wajiha Mahjabeen, Neerupma Silswal and Nilofer Qureshi

Title	Expression of inflammasome-related genes in bladder cancer and their association with cytokeratin 20 messenger RNA
Journal	Urologic Oncology. 2015.
Authors	Giulia Poli, B.Sc., Stefano Brancorsini, Ph.D., Giovanni Cochetti, M.D., Ph.D., Francesco Barillaro, M.D., Maria Giulia Egidi, Ph.D., Ettore Mearini, M.D., Ph.D.

Title	Analysis of Plasma MicroRNA Expression Profiles in Male Textile Workers with Noise-induced Hearing Loss
Journal	Hearing Research. 2015.
Authors	Lu Ding, Jing Liu, Huan-Xi Shen, Li-Ping Pan, Qing-Dong Liu, Heng-Dong Zhang, Lei Han, Li-Guo Shuai, En-Min Ding, Qiu-Ni Zhao, Bo-Shen Wang, Bao-Li Zhu

Title	Integrating microbial and host transcriptomics to characterize asthma-associated microbial communities
Journal	BMC Medical Genomics. 2015.
Authors	Eduardo Castro-Nallar, Ying Shen, Robert J. Freishtat, Marcos Pérez-Losada, Solaiappan Manimaran, Gang Liu, W. Evan Johnson and Keith A. Crandall

Title	Inhibition of miR-29 has a significant lipid-lowering benefit through suppression of lipogenic programs in live
Journal	Scientific Reports. 2015.
Authors	C. Lisa Kurtz, Emily E. Fannin, Cynthia L. Toth, Daniel S. Pearson, Kasey C. Vickers & Praveen Sethupathy

Title	Transcriptome dynamics of developing photoreceptors in 3-D retina cultures recapitulates temporal sequence of human cone and rod differentiation revealing cell surface markers and gene networks.
Journal	Stem Cells. 2015.
Authors	Kaewkhaw R, Kaya KD, Brooks M, Homma K, Zou J, Chaitankar V, Rao M, Swaroop A

Title	Pyrvinium targets CD133 in human glioblastoma brain tumor-initiating cells
Journal	Clinical Cancer Research. 2015.
Authors	Sheila K Singh, Chitra Venugopal, Robin Hallett, Parvez Vora, Branavan Manoranjan, Sujeivan Mahendram, Maleeha Qazi, Nicole McFarlane, Minomi Subapanditha, Sara Nolte, Mohini Singh, David Bakhshinyan, Neha Garg, Thusyanth Vijayakumar, Boleslaw Lach, John P Provias, Kesh Reddy, Naresh Murty, Bradley Doble, Mickie Bhatia, and John A Hassell

Title	MicroRNAs Classify Different Disease Behavior Phenotypes of Crohn's Disease and May Have Prognostic Utility
Journal	Inflammatory Bowel Diseases. 2015.
Authors	Peck, Bailey C. E. BA; Weiser, Matthew BS; Lee, Saangyoung E. BS; Gipson, Gregory R. BS; Iyer, Vishal B.; Sartor, Ryan B. MD; Herfarth, Hans H. MD, PhD; Long, Millie D. MD, MPH; Hansen, Jonathan J. MD, PhD; Isaacs, Kim L. MD, PhD; Trembath, Dimitri G. MD; Rahbar, Reza MD; Sadiq, Timothy S. MD; Furey, Terrence S. PhD; Sethupathy, Praveen PhD; Sheikh, Shehzad Z. MD, PhD

Title	LIN28A modulates splicing and gene expression programs in breast cancer cells
Journal	Molecular and Cellular Biology. 2015.
Authors	Jun Yang, Brian D. Bennett, Shujun Luo, Kaoru Inoue, Sara A. Grimm, Gary P. Schroth, Pierre R. Bushel, H. Karimi Kinyamu and Trevor K. Archer

Title	A predictive biophysical model of translational coupling to coordinate and control protein expression in bacterial operons
Journal	Nucleic Acids Research. 2015.
Authors	Tian Tian and Howard M. Salis

Title	Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue
Journal	Nature Communications. 2015.
Authors	James P. K. Armstrong, Rameen Shakur, Joseph P. Horne, Sally C. Dickinson, Craig T. Armstrong, Katherine Lau, Juned Kadiwala, Robert Lowe, Annela Seddon, Stephen Mann, J. L. Ross Anderson, Adam W. Perriman & Anthony P. Hollander

Title	The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer
Journal	Oncotarget. 2015.
Authors	Kogo R, How C, Chaudary N, Bruce J, Shi W, Hill RP, Zahedi P, Yip KW, Liu FF

Title	Dual Transcriptomic Profiling of Host and Microbiota during Health and Disease in Pediatric Asthma
Journal	PLOS ONE. 2015.
Authors	Marcos Pérez-Losada, Eduardo Castro-Nallar, Matthew L. Bendall, Robert J. Freishtat, Keith A. Crandall

Title	Mitochondrial complex I and III mRNA levels in bipolar disorder
Journal	Journal of Affective Disorders. 2015.
Authors	Akarsu S, Torun D, Erdem M, Kozan S, Akar H, Uzun O

Title	Transcriptome and H3K27 tri-methylation profiling of Ezh2-deficient lung epithelium
Journal	Genomics Data. 2015.
Authors	Aliaksei Z. Holik, Laura A. Galvis, Aaron T.L. Lun, Matthew E. Ritchie, Marie-Liesse Asselin-Labat

Title	AtPGL3 is an Arabidopsis BURP domain protein that is localized to the cell wall and promotes cell enlargement
Journal	Frontiers in Plant Science. 2015.
Authors	Jiyoung Park, Yong Cui and Byung-Ho Kang

Title	Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites
Journal	eLIFE. 2015.
Authors	Yong H Woo, Hifzur Ansari, Thomas D Otto, Christen M Klinger, Martin Kolisko, Jan Michálek, Alka Saxena, Dhanasekaran Shanmugam, Annageldi Tayyrov, Alaguraj Veluchamy, Shahjahan Ali, Axel Bernal, Javier del Campo, Jaromír Cihlář, Pavel Flegontov, Sebastian G Gornik, Eva Hajdušková, Aleš Horák, Jan Janouškovec, Nicholas J Katris, Fred D Mast, Diego Miranda-Saavedra, Tobias Mourier, Raeece Naeem, Mridul Nair, Aswini K Panigrahi, Neil D Rawlings, Eriko Padron-Regalado, Abhinay Ramaprasad, Nadira Samad, Aleš Tomčala, Jon Wilkes, Daniel E Neafsey, Christian Doerig, Chris Bowler, Patrick J Keeling, David S Roos, Joel B Dacks, Thomas J Templeton, Ross F Waller, Julius Lukeš, Miroslav Oborník, Arnab Pain

Title	Dose-dependent Modulation of Lipid Parameters, Cytokines and RNA by δ-tocotrienol in Hypercholesterolemic Subjects Restricted to AHA Step-1 Diet
Journal	British Journal of Medicine & Medical Research. 2015.
Authors	Asaf A. Qureshi, Dilshad A. Khan, Wajiha Mahjabeen and Nilofer Qureshi

Title	CD24 ⁺ cells fuel rapid tumor growth and display high metastatic capacity
Journal	Breast Cancer Research. 2015.
Authors	Ran Rostoker, Sagi Abelson, Inna Genkin, Sarit Ben-Shmuel, Ravi Sachidanandam, Eyal J. Scheinman, Keren Bitton-Worms, Zila Shen Orr, Avishay Caspi, Maty Tzukerman and Derek LeRoith

Title	Genes involved in sex pheromone biosynthesis of Ephestia cautella, an important food storage pest, are determined by transcriptome sequencing
Journal	BMC Genomics. 2015.
Authors	Binu Antony, Alan Soffan, Jernej Jakše, Sulieman Alfaifi, Koko D. Sutanto, Saleh A. Aldosari, Abdulrahman S. Aldawood and Arnab Pain

Title	Genetic population structure of the invasive ash dieback pathogen Hymenoscyphus fraxineus in its expanding range
Journal	Biological Invasions. 2015.
Authors	Daiva Burokiene, Simone Prospero, Esther Jung, Diana Marciulyniene, Karin Moosbrugger, Goda Norkute, Daniel Rigling, Vaidotas Lygis, Corine N. Schoebel

Title	Endogenous and Uric Acid-Induced Activation of NLRP3 Inflammasome in Pregnant Women with Preeclampsia
Journal	PLOS ONE. 2015.
Authors	Mariana Leticia Matias, Mariana Romão, Ingrid Cristina Weel, Vanessa Rocha Ribeiro, Priscila Rezeck Nunes, Vera Therezinha Borges, João Pessoa Araújo, Jr, José Carlos Peraçoli, Leandro de Oliveira, Maria Terezinha Peraçoli

Title	Insights into Transcriptomes of Big and Low Sagebrush
Journal	PLOS ONE. 2015.
Authors	Mark D. Huynh, Justin T. Page, Bryce A. Richardson, Joshua A. Udall

Title	Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications
Journal	Disease Markers. 2015.
Authors	Maria Giulia Egidi, Giovanni Cochetti, Gabriella Guelfi, Danilo Zampini, Silvana Diverio, Giulia Poli, and Ettore Mearini

Title	Deciphering intracellular events triggered by mild magnetic hyperthermia in vitro and in vivo.
Journal	Nanomedicine. 2015.
Authors	Moros M, Ambrosone A, Stepien G, Fabozzi F, Marchesano V, Castaldi A, Tino A, de la Fuente JM, Tortiglione C.

Title	Characterization of Kallireins and microRNAs in Urine Sediment for the Discrimination of Prostate Cancer from Benign Prostatic Hyperplasia
Journal	Cancer & Science Therapy. 2015.
Authors	Maria G. Egidi, Gabriella Guelfi, Giovanni Cochetti, Giulia Poli, Francesco Barillaro, Danilo Zampini, Luca Mechelli and Ettore Mearini

Title	Detection and quantification of microRNA in cerebral microdialysate
Journal	Journal of Translational Medicine. 2015.
Authors	Søren Bache, Rune Rasmussen, Maria Rossing, Niels Risør Hammer, Marianne Juhler, Lennart Friis-Hansen, Finn Cilius Nielsen, Kirsten Møller

Title	Chromosomal instability as a prognostic marker in cervical cancer
Journal	BMC Cancer. 2015.
Authors	Christine How, Jeff Bruce, Jonathan So, Melania Pintilie, Benjamin Haibe-Kains, Angela Hui, Blaise A Clarke, David W Hedley, Richard P Hill, Michael Milosevic, Anthony Fyles, Fei-Fei Liu

Title	Endogenous tRNA-Derived Fragments Suppress Breast Cancer Progression via YBX1 Displacement
Journal	Cell. 2015.
Authors	Hani Goodarzi, Xuhang Liu, Hoang C.B. Nguyen, Steven Zhang, Lisa Fish, Sohail F. Tavazoie

Title	Effects of Protein A in Detection of Canine Distemper Virus through immunosensor construction
Journal	IEEE Sensors Journal. 2015.
Authors	Caroline Rodrigues Basso, Juliane R. Sempionatto, Claudia C. Tozato, Gustavo C. Rocha, João Pessoa Araujo Junior, Valber A. Pedrosa

Title	The Notch ligand DLL4 specifically marks human hematoendothelial progenitors and regulates their hematopoietic fate
Journal	Leukemia. 2015.
Authors	V Ayllón, C Bueno, V Ramos-Mejía, O Navarro-Montero, C Prieto, P J Real, T Romero, M J García-León, M L Toribio, A Bigas and P Menendez

Title	MicroRNA expression profile of surgical removed mandibular bone tissues from patients with mandibular prognathism
Journal	Journal of Surgical Research. 2015.
Authors	Yulou Tian, Jie Liu, Xiaofeng Bai, Xuexin Tan, Yuming Cao, Ke Qin, Zhenjin Zhao,Yuanyuan Zhang

Title	Epstein-Barr Virus microRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival
Journal	EBioMedicine. 2015.
Authors	Alessandra Ferrajoli, Cristina Ivan, Maria Ciccone, Masayoshi Shimizu, Yoshiaki Kita, Masahisha Ohtsuka, Lucilla D’Abundo, Jun Qiang, Susan Lerner, Nazila Nouraee, Kari G. Rabe, Laura Z. Rassenti, Katrien Van Roosbroeck, John T. Manning, Yuan Yuan, Xinna Zhang, Tait D. Shanafelt, William G. Wierda, Silvia Sabbioni, Jeffrey J. Tarrand, Zeev Estrov, Milan Radovich, Han Liang, Massimo Negrini, Thomas J. Kipps, Neil E. Kay, Michael Keating, George A. Calin.

Title	Why weight? Modelling sample and observational level variability improves power in RNA-seq analyses
Journal	Nucleic Acids Research. 2015.
Authors	Ruijie Liu, Aliaksei Z. Holik, Shian Su, Natasha Jansz, Kelan Chen, Huei San Leong, Marnie E. Blewitt, Marie-Liesse Asselin-Labat, Gordon K. Smyth and Matthew E. Ritchie

Title	Role of MicroRNAs in Progression and Recurrence of Early-Stage Lung Adenocarcinoma
Journal	Pulmonary Research and Respiratory Medicine. 2015.
Authors	Rama K. Singh, Drew C. Bethune, Zhaolin Xu and Susan E. Douglas

Title	APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages
Journal	Nature Communications. 2015.
Authors	Shraddha Sharma, Santosh K. Patnaik, R. Thomas Taggart, Eric D. Kannisto, Sally M. Enriquez, Paul Gollnick & Bora E. Baysal

Title	Comparative clinical sample preparation of DNA and RNA viral nucleic acids for a commercial deep sequencing system (Illumina MiSeq®)
Journal	Journal of Virological Methods. 2015.
Authors	Leila Sabrina Ullmann, Claudia de Camargo Tozato, Camila Dantas Malossi, Tais Fukuta da Cruz, Raissa Vasconcelos Cavalcante, Jacqueline Kazue Kurissio, Didier Quevedo Cagnini, Marianna Vaz Rodrigues, Alexander Welker Biondo, Joao Pessoa Araujo Jr

Title	Developing a Prognostic Micro-RNA Signature for Human Cervical Carcinoma
Journal	PLOS One. 2015.
Authors	Christine How, Melania Pintilie, Jeff P. Bruce, Angela B. Y. Hui, Blaise A. Clarke, Philip Wong, Shaoming Yin, Rui Yan, Daryl Waggott, Paul C. Boutros, Anthony Fyles, David W. Hedley, Richard P. Hill, Michael Milosevic, Fei-Fei Liu

Title	Na⁺/H⁺ Exchanger Isoform 1-Induced Osteopontin Expression Facilitates Cardiomyocyte Hypertrophy
Journal	PLOS One. 2015.
Authors	Iman A. Mohamed, Alain-Pierre Gadeau, Larry Fliegel, Gary Lopaschuk, Mohamed Mlih, Nabeel Abdulrahman, Natasha Fillmore, Fatima Mraiche

Title	Identification of molecular determinants of primary and metastatic tumour re-initiation in breast cancer
Journal	Nature Cell Biology. 2015.
Authors	Jason B. Ross, Doowon Huh, Lisa B. Noble and Sohail F. Tavazoie

Title	Multi-Lineage Differentiation of Human Umbilical Cord Wharton’s Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers
Journal	PLOS ONE. 2015.
Authors	Hamad Ali, Majda K. Al-Yatama, Mohamed Abu-Farha, Kazem Behbehani, Ashraf Al Madhoun

Title	Induction of lcc2 expression and activity by Agaricus bisporus provides defence against Trichoderma aggressivum toxic extracts
Journal	Microbial Biotechnology. 2015.
Authors	Calvin P. Sjaarda, Kamal S. Abubaker and Alan J. Castle

Title	Early handling and repeated cross-fostering have opposite effect on mouse emotionality
Journal	Frontiers in Behavioral Science. 2015.
Authors	Alessandra Luchetti, Diego Oddi, Valentina Lampis, Eleonora Centofante, Armando Felsani, Marco Battaglia and Francesca R D_Amato

Title	Na⁺/H⁺ Exchanger Isoform 1 Induced Cardiomyocyte Hypertrophy Involves Activation of p90 Ribosomal S6 Kinase
Journal	PLOS ONE. 2015.
Authors	Maiy Jaballah, Iman A. Mohamed, Bayan Alemrayat, Fatima Al-Sulaiti, Mohamed Mlih, Fatima Mraiche

Title	Silver-Zinc Redox-Coupled Electroceutical Wound Dressing Disrupts Bacterial Biofilm
Journal	PLoS ONE. 2015.
Authors	Jaideep Banerjee, Piya Das Ghatak, Sashwati Roy, Savita Khanna, Craig Hemann, Binbin Deng, Amitava Das, Jay L. Zweier, Daniel Wozniak, Chandan K. Sen

Title	MiR-377 targets E2F3 and alters the NF-kB signaling pathway through MAP3K7 in malignant melanoma
Journal	Molecular Cancer. 2015.
Authors	Liron Zehavi1, Hagit Schayek, Jasmine Jacob-Hirsch, Yechezkel Sidi, Raya Leibowitz-Amit and Dror Avni

Title	Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung
Journal	Development. 2015.
Authors	Laura A. Galvis, Aliaksei Z. Holik, Kieran M. Short, Julie Pasquet, Aaron T. L. Lun, Marnie E. Blewitt, Ian M. Smyth, Matthew E. Ritchie and Marie-Liesse Asselin-Labat

Title	Phosphorylation-dependent degradation of MEF2C contributes to regulate G2/M transition
Journal	Cell Cycle. 2015.
Authors	Sara Badodi, Fiorenza Baruffaldi, Massimo Ganassi, Renata Battini & Susanna Molinari

Title	Highly specific role of the insulin receptor in breast cancer progression
Journal	Endocrine-Related Cancer. 2015.
Authors	Ran Rostoker, Sagi Abelson, Keren Bitton-Worms, Inna Genkin, Sarit Ben-Shmuel, Maria Dakwar, Zila Shen Orr, Avishay Caspi, Maty Tzukerman and Derek LeRoith

Title	N6-methyladenosine marks primary microRNAs for processing
Journal	Nature. 2015.
Authors	Claudio R. Alarcon, Hyeseung Lee, Hani Goodarzi, Nils Halberg & Sohail F. Tavazoie

Title	eIF6 over-expression increases the motility and invasiveness of cancer cells by modulating the expression of a critical subset of membrane-bound proteins
Journal	BMC Cancer. 2015.
Authors	Michela Pinzaglia, Claudia Montaldo, Dorina Polinari, Mattei Simone, Anna La Teana, Marco Tripodi, Carmine Mancone, Paola Londei, Dario Benelli

Title	Examining the Feasibility of Clinical Grade CD271+ Enrichment of Mesenchymal Stromal Cells for Bone Regeneration
Journal	PLoS ONE. 2015.
Authors	Richard J. Cuthbert, Peter V. Giannoudis, Xiao N. Wang, Lindsay Nicholson, David Pawson, Anatole Lubenko, Hiang B. Tan, Anne Dickinson, Dennis McGonagle, Elena Jones

Title	Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved
Journal	Journal of Biotechnology. 2015.
Authors	Andrea Bedford, Tao Chen, Evanna Huynh, Cuilan Zhu, Samantha Medeiros, Doug Wey, Cornelis de Lange, Julang Li

Title	Results, Questions, Perspectives of a Study on Human Polyomavirus BK and Molecular Actors in Prostate Cancer Development
Journal	Cancer Genomics Proteomics. 2015.
Authors	Mischitelli M, Bellizzi A, Anzivino E, Rodio DM, Sciarra A, Gentile V, Pietropaolo V

Title	Isolation and expression of a cadmium-resistant gene (SaFer) from Sedum alfredii
Journal	Journal of Zhejiang A and F University. 2015.
Authors	ZHAO Ting, HAN Xiaojiao, LIU Mingying, QIAO Guirong, JIANG Jing, JIANG Yancheng,ZHUO Renying

Title	Epigenetic biomarkers in the blood of patients with urological malignancies
Journal	Expert Review of Molecular Diagnostics. 2015.
Authors	Jörg Ellinger, Stefan C Müller, and Dimo Dietrich

Title	In vitro cytotoxicity analysis of doxorubicin-loaded/superparamagnetic iron oxide colloidal nanoassemblies on MCF7 and NIH3T3 cell lines
Journal	International Journal of Nanomedicine. 2015.
Authors	Katerina Tomankova, Katerina Polakova, Klara Pizova, Svatopluk Binder, Marketa Havrdova, Mary Kolarova, Eva Kriegova, Jana Zapletalova, Lukas Malina, Jana Horakova, Jakub Malohlava, Argiris Kolokithas-Ntoukas, Aristides Bakandritsos, Hana

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Total RNA Purification Kits Dx (Cat. Dx17200, Dx24300, Dx24350, Dx24380)

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.