Total RNA Purification Micro Kit (Cat. 35300, 35350)
Total RNA Purification Micro Kit
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*Inquire by phone or email for more information.
What Scientists are Saying About this Product
In general, we struggled to find the best RNA purification kit for cells cultured in or within biomaterials. The Total RNA Purification Micro Kit has consistently been a great product for us! We are able to get good quality RNA for smaller samples enabling us to run PCR on those samples. Other kits have resulted in really low concentration/quantity of RNA or really poor quality RNA. This kit also saves us time because we are typically able to lysis the cell-material construct directly instead of needing to try to collect the cells first before lysing. All in all, I've been using these kits since I was a postdoc and am very happy to continue using them in my own research group.
- Jessica Gluck
Total RNA Purification Micro Kit
Rapid purification of total RNA - including microRNA - from small input amounts
- Extract high quality & quantity total RNA including miRNA
- No phenol step required - isolate all RNA in one fraction
- Elute extracted total RNA in small volume (20 µL)
- Bind & elute all RNA irrespective of size or GC content, without bias.
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 35300) 50 preps
(Cat. 35350) 250 Preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
35 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
| Minimum Elution Volume |
20 μL
|
| Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
|
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) |
5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells |
| Time to Complete 10 Purifications |
20 minutes
|
|
Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
35 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
| Minimum Elution Volume |
20 μL
|
| Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
|
Maximum Amount of Starting Material: Heart, muscle |
5 x 105 cells
3 mg (for most tissues*) up to 7.5 mL
Up to 5 x 105 cells |
| Time to Complete 10 Purifications |
20 minutes
|
|
Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
Micro (35300, 35350)
| Title | Molecular diagnosis of SARS-CoV-2 in seminal fluid |
| Journal | Journal of Endocrinological Investigation. 2021. |
| Authors | D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo |
| Title | Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications |
| Journal | Journal of Biomedical Materials Research. 2017. |
| Authors | Blanquer A, Musilkova J, Barrios L, Ibáñez E, Vandrovcova M, Pellicer E, Sort J, Bacakova L, Nogués C |
| Title | Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome |
| Journal | Journal of Clinical Investigation. 2016. |
| Authors | Burnett LC, LeDuc CA, Sulsona CR, Paull D, Rausch R, Eddiry S, Carli JF, Morabito MV, Skowronski AA, Hubner G, Zimmer M, Wang L, Day R, Levy B, Fennoy I, Dubern B, Poitou C, Clement K, Butler MG, Rosenbaum M, Salles JP, Tauber M, Driscoll DJ, Egli D, Leibel RL |
| Title | Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies |
| Journal | International Journal of Molecular Sciences. 2016. |
| Authors | Ma Y, Luk A, Young FP, Lynch D, Chua W, Balakrishnar B, de Souza P, Becker TM |
| Title | Tumor necrosis factor / sphingosine-1-phosphate signaling augments resistance artery myogenic tone in diabetes |
| Journal | Diabetes. 2016. |
| Authors | Sauvé M, Hui SK, Dinh DD, Foltz WD, Momen A, Nedospasov SA, Offermanns S, Husain M, Kroetsch JT, Lidington D, Bolz SS |
| Title | The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering |
| Journal | Int J Nanomedicine. 2016. |
| Authors | Bacakova, M., Musilkova, J., Riedel, T., Stranska, D., Brynda, E., Zaloudkova, M., & Bacakova, L |
| Title | Sphingosine-1-Phosphate Signaling Regulates Myogenic Responsiveness in Human Resistance Arteries |
| Journal | PLOS ONE. 2015. |
| Authors | Sonya Hui, Andrew S. Levy, Daniel L. Slack, Marcus J. Burnstein, Lee Errett, Daniel Bonneau, David Latter, Ori D. Rotstein, Steffen-Sebastian Bolz , Darcy Lidington, Julia Voigtlaender-Bolz |
| Title | Scleraxis is required for the development of a functional tendon enthesis |
| Journal | FASEB Journal. 2015. |
| Authors | Killian ML, Thomopoulos S |
| Title | Cell adhesion and growth enabled by biomimetic oligopeptide modification of a polydopamine-poly(ethylene oxide) protein repulsive surface |
| Journal | Journal of Materials Science: Materials in Medicine. 2015. |
| Authors | Jana Musilkova, Ilya Kotelnikov, Katarina Novotna, Ognen Pop-Georgievski, Frantisek Rypacek, Lucie Bacakova, Vladimir Proks |
| Title | Hypoxia-inducible factor 3-alpha expression is associated with the stable chondrocyte phenotype |
| Journal | Journal of Orthopaedic Research. 2015. |
| Authors | Brandon D. Markway, Holly Cho, Jevgenia Zilberman-Rudenko, Paul Holden, Audrey McAlinden and Brian Johnstone |
| Title | Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion |
| Journal | Nature Medicine. 2015. |
| Authors | Caroline Bonner, Julie Kerr-Conte, Valéry Gmyr, Gurvan Queniat, Ericka Moerman, Julien Thévenet, Cédric Beaucamps, Nathalie Delalleau, Iuliana Popescu, Willy J Malaisse, Abdullah Sener, Benoit Deprez, Amar Abderrahmani, Bart Staels & François Pattou |
| Title | Enthesis fibrocartilage cells originate from a population of Hedgehog-responsive cells modulated by the loading environment |
| Journal | Development. 2015. |
| Authors | Schwartz AG, Long F, Thomopoulos S |
| Title | Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum free embryoid bodies. |
| Journal | American Journal of Physiology - Cell Physiology. 2014. |
| Authors | Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Pre, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle. |
| Title | Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline. |
| Title | Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline. |
| Title | Immunoresolving actions of oral resolvin D1 include selective regulation of the transcription machinery in resolution-phase mouse macrophages. |
| Journal | FASEB Journal. 2014. |
| Authors | Recchiuti A, Codagnone M, Pierdomenico AM, Rossi C, Mari VC, Cianci E, Simiele F, Gatta V, Romano M. |
| Title | Differentially Expressed MicroRNAs in Chondrocytes from Distinct Regions of Developing Human Cartilage. |
| Journal | PLoS One. 2013. |
| Authors | McAlinden A, Varghese N, Wirthlin L, Chang LW. |
Total RNA Purification Micro Kit
This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications.
This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
| Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
|---|---|---|---|---|---|
| 55000 | Plasma/Serum RNA Purification Mini Kit | 10 - 25 µL | 50 µL - 1 mL | 250 µL - 1 mL | 5 - 10 mL |
| 35300 | Total RNA Purification Micro Kit | 20 - 50 µL | 1 - 4 mL | 2 - 10 mL | 10 - 20 mL |
| 17200 | Total RNA Purification Kit | 50 - 100 µL | 4 - 10 mL | 11 - 30 mL | 20 - 35 mL |
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
35 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
| Minimum Elution Volume |
20 μL
|
| Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
|
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) |
5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells |
| Time to Complete 10 Purifications |
20 minutes
|
|
Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
35 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
| Minimum Elution Volume |
20 μL
|
| Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
|
Maximum Amount of Starting Material: Heart, muscle |
5 x 105 cells
3 mg (for most tissues*) up to 7.5 mL
Up to 5 x 105 cells |
| Time to Complete 10 Purifications |
20 minutes
|
|
Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Micro (35300, 35350)
| Title | Molecular diagnosis of SARS-CoV-2 in seminal fluid |
| Journal | Journal of Endocrinological Investigation. 2021. |
| Authors | D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo |
| Title | Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications |
| Journal | Journal of Biomedical Materials Research. 2017. |
| Authors | Blanquer A, Musilkova J, Barrios L, Ibáñez E, Vandrovcova M, Pellicer E, Sort J, Bacakova L, Nogués C |
| Title | Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome |
| Journal | Journal of Clinical Investigation. 2016. |
| Authors | Burnett LC, LeDuc CA, Sulsona CR, Paull D, Rausch R, Eddiry S, Carli JF, Morabito MV, Skowronski AA, Hubner G, Zimmer M, Wang L, Day R, Levy B, Fennoy I, Dubern B, Poitou C, Clement K, Butler MG, Rosenbaum M, Salles JP, Tauber M, Driscoll DJ, Egli D, Leibel RL |
| Title | Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies |
| Journal | International Journal of Molecular Sciences. 2016. |
| Authors | Ma Y, Luk A, Young FP, Lynch D, Chua W, Balakrishnar B, de Souza P, Becker TM |
| Title | Tumor necrosis factor / sphingosine-1-phosphate signaling augments resistance artery myogenic tone in diabetes |
| Journal | Diabetes. 2016. |
| Authors | Sauvé M, Hui SK, Dinh DD, Foltz WD, Momen A, Nedospasov SA, Offermanns S, Husain M, Kroetsch JT, Lidington D, Bolz SS |
| Title | The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering |
| Journal | Int J Nanomedicine. 2016. |
| Authors | Bacakova, M., Musilkova, J., Riedel, T., Stranska, D., Brynda, E., Zaloudkova, M., & Bacakova, L |
| Title | Sphingosine-1-Phosphate Signaling Regulates Myogenic Responsiveness in Human Resistance Arteries |
| Journal | PLOS ONE. 2015. |
| Authors | Sonya Hui, Andrew S. Levy, Daniel L. Slack, Marcus J. Burnstein, Lee Errett, Daniel Bonneau, David Latter, Ori D. Rotstein, Steffen-Sebastian Bolz , Darcy Lidington, Julia Voigtlaender-Bolz |
| Title | Scleraxis is required for the development of a functional tendon enthesis |
| Journal | FASEB Journal. 2015. |
| Authors | Killian ML, Thomopoulos S |
| Title | Cell adhesion and growth enabled by biomimetic oligopeptide modification of a polydopamine-poly(ethylene oxide) protein repulsive surface |
| Journal | Journal of Materials Science: Materials in Medicine. 2015. |
| Authors | Jana Musilkova, Ilya Kotelnikov, Katarina Novotna, Ognen Pop-Georgievski, Frantisek Rypacek, Lucie Bacakova, Vladimir Proks |
| Title | Hypoxia-inducible factor 3-alpha expression is associated with the stable chondrocyte phenotype |
| Journal | Journal of Orthopaedic Research. 2015. |
| Authors | Brandon D. Markway, Holly Cho, Jevgenia Zilberman-Rudenko, Paul Holden, Audrey McAlinden and Brian Johnstone |
| Title | Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion |
| Journal | Nature Medicine. 2015. |
| Authors | Caroline Bonner, Julie Kerr-Conte, Valéry Gmyr, Gurvan Queniat, Ericka Moerman, Julien Thévenet, Cédric Beaucamps, Nathalie Delalleau, Iuliana Popescu, Willy J Malaisse, Abdullah Sener, Benoit Deprez, Amar Abderrahmani, Bart Staels & François Pattou |
| Title | Enthesis fibrocartilage cells originate from a population of Hedgehog-responsive cells modulated by the loading environment |
| Journal | Development. 2015. |
| Authors | Schwartz AG, Long F, Thomopoulos S |
| Title | Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum free embryoid bodies. |
| Journal | American Journal of Physiology - Cell Physiology. 2014. |
| Authors | Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Pre, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle. |
| Title | Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline. |
| Title | Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline. |
| Title | Immunoresolving actions of oral resolvin D1 include selective regulation of the transcription machinery in resolution-phase mouse macrophages. |
| Journal | FASEB Journal. 2014. |
| Authors | Recchiuti A, Codagnone M, Pierdomenico AM, Rossi C, Mari VC, Cianci E, Simiele F, Gatta V, Romano M. |
| Title | Differentially Expressed MicroRNAs in Chondrocytes from Distinct Regions of Developing Human Cartilage. |
| Journal | PLoS One. 2013. |
| Authors | McAlinden A, Varghese N, Wirthlin L, Chang LW. |
