Total RNA Purification Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts Price USD$439.00 SKU 35300 Format Micro Size 50 Preps 250 Preps Quantity Overview Details Documentation Citations Overview Extract high quality & quantity total RNA including miRNA No phenol step required - isolate all RNA in one fraction Elute extracted total RNA in small volume (20 µL) Bind & elute all RNA irrespective of size or GC content, without bias. Efficiently extract small RNA irrespective of GC content Very sensitive & linear down to a few cells without the need for carrier RNA Convenient & fast spin column format Isolate from a wide variety of specimens Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications. This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content. Isolate RNA after Purifying EVs and Exosomes Ultracentrifugation, Exoquick, Filtration Cat. # Name Elution Volume Plasma/Serum Urine Cell-Culture Media 55000 Plasma/Serum RNA Purification Mini Kit 10 - 25 µL 50 µL - 1 mL 250 µL - 1 mL 5 - 10 mL 35300 Total RNA Purification Micro Kit 20 - 50 µL 1 - 4 mL 2 - 10 mL 10 - 20 mL 17200 Total RNA Purification Kit 50 - 100 µL 4 - 10 mL 11 - 30 mL 20 - 35 mL Details Supporting Data Previous Figure 1. Great Isolation Sensitivity. Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells. Five µL of the eluted RNA was used as the template in a 20 µL RT-qPCR reaction to detect the human S14 transcript. The S14 was detected from as little as a single cell. Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM). Norgen's Total RNA Purification Micro Kit allows sensitive but high quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen's Total RNA Purification Micro Kit recovered good amounts of RNA with excellent 260/280 ratio. Figure 3. Better Efficiency of RNA Recovery at Single Cell Input. Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor's kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen's Total RNA Purification Micro Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript transcript in the isolated RNA. RNA isolated by Norgen's Total RNA Purification Micro Kit showed much higher relative expression of each RNA transcript tested at all cell input number down to single cell. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 35 μg Maximum Column Loading Volume 650 μL Minimum Elution Volume 20 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) 5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells Time to Complete 10 Purifications 20 minutes Average Yield HeLa Cells (1 x 105 cells) ~1.5 μg * for fibrous tissue, an additional Proteinase K treatment is required Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 35300 (50 preps) Cat. 35350 (250 preps) Buffer RL 40 mL 5 x 40 mL Wash Solution A 38 mL 5 x 38 mL Elution Solution A 6 mL 5 x 6 mL Micro Spin Columns 50 250 Collection Tubes 50 250 Elution Tubes (1.7 mL) 50 250 Product Insert 1 1 Documentation PROTOCOLS (35300, 35350) Total RNA Purification Micro Kit - Protocol (50 and 250 prep) SUPPLEMENTARY PROTOCOLS Total RNA Purification Micro Kit Supplementary Protocol For Dried Blood Spot RNA Isolation - Supplementary Protocol Total RNA Purification Micro Kit - Supplementary Protocol for Exosomal RNA Purification from Exosomes Already Purified SAFETY DATA SHEETS (35300, 35350) Total RNA Purification Micro Kit - Safety Data Sheet POSTERS Explore the World of RNA - Silicon-Carbide vs. Silica-min.pdf FLYERS Total RNA Purification Micro Kit RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Services NGS RNA Isolation Related Products Animal Tissue RNA Purification Kit Leukocyte RNA Purification Kit 96-Well Kit Plant/Fungi Total RNA Purification Kit RNase-Free DNase I Kit Total RNA Purification Kit Total RNA Purification Plus Kit Total RNA Purification Plus Micro Kit
Total RNA Purification Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts Price USD$439.00 SKU 35300 Format Micro Size 50 Preps 250 Preps Quantity
