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Total RNA Purification Micro Kit

Cat. 35300, 35350
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Total RNA Purification Micro Kit

Cat. 35300, 35350

Rapid purification of total RNA - including microRNA - from small input amounts

  • Extract high quality & quantity total RNA including miRNA
  • No phenol step required - isolate all RNA in one fraction
  • Elute extracted total RNA in small volume (20 µL)
  • Bind & elute all RNA irrespective of size or GC content, without bias.
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Total RNA Purification Micro Kit
(Cat. 35300)
50 preps
Total RNA Purification Micro Kit (250)
(Cat. 35350)
250 Preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 35300
Kit Specifications
Maximum Column Binding Capacity
35 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume
20 μL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)

Maximum Amount of Starting Material:

Animal Cells

Animal Tissues (Brain, Kidney, Liver, Lung, Spleen)

Heart, muscle 

Laser-Captured Microdissection (LCM)



5 x 105 cells

3 mg (for most tissues*)


up to 7.5 mL

Up to 5 x 105 cells

Time to Complete 10 Purifications
20 minutes
Average Yield
HeLa Cells (1 x 105 cells)
 
~1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 35350
Kit Specifications
Maximum Column Binding Capacity
35 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume
20 μL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)

Maximum Amount of Starting Material:
Animal Cells
Animal Tissues (Brain, Kidney, Liver, Lung, Spleen)

Heart, muscle 
Laser-Captured Microdissection (LCM)

5 x 105 cells
 

3 mg (for most tissues*)
up to 7.5 mL
Up to 5 x 105 cells
Time to Complete 10 Purifications
20 minutes
Average Yield
HeLa Cells (1 x 105 cells)
 
~1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Micro (35300, 35350)

Title Molecular diagnosis of SARS-CoV-2 in seminal fluid
Journal Journal of Endocrinological Investigation. 2021.
Authors D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo
Title Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications
Journal Journal of Biomedical Materials Research. 2017.
Authors Blanquer A, Musilkova J, Barrios L, Ibáñez E, Vandrovcova M, Pellicer E, Sort J, Bacakova L, Nogués C
Title Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome
Journal Journal of Clinical Investigation. 2016.
Authors Burnett LC, LeDuc CA, Sulsona CR, Paull D, Rausch R, Eddiry S, Carli JF, Morabito MV, Skowronski AA, Hubner G, Zimmer M, Wang L, Day R, Levy B, Fennoy I, Dubern B, Poitou C, Clement K, Butler MG, Rosenbaum M, Salles JP, Tauber M, Driscoll DJ, Egli D, Leibel RL
Title Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies
Journal International Journal of Molecular Sciences. 2016.
Authors Ma Y, Luk A, Young FP, Lynch D, Chua W, Balakrishnar B, de Souza P, Becker TM
Title Tumor necrosis factor / sphingosine-1-phosphate signaling augments resistance artery myogenic tone in diabetes
Journal Diabetes. 2016.
Authors Sauvé M, Hui SK, Dinh DD, Foltz WD, Momen A, Nedospasov SA, Offermanns S, Husain M, Kroetsch JT, Lidington D, Bolz SS
Title The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
Journal Int J Nanomedicine. 2016.
Authors Bacakova, M., Musilkova, J., Riedel, T., Stranska, D., Brynda, E., Zaloudkova, M., & Bacakova, L
Title Sphingosine-1-Phosphate Signaling Regulates Myogenic Responsiveness in Human Resistance Arteries
Journal PLOS ONE. 2015.
Authors Sonya Hui, Andrew S. Levy, Daniel L. Slack, Marcus J. Burnstein, Lee Errett, Daniel Bonneau, David Latter, Ori D. Rotstein, Steffen-Sebastian Bolz , Darcy Lidington, Julia Voigtlaender-Bolz
Title Scleraxis is required for the development of a functional tendon enthesis
Journal FASEB Journal. 2015.
Authors Killian ML, Thomopoulos S
Title Cell adhesion and growth enabled by biomimetic oligopeptide modification of a polydopamine-poly(ethylene oxide) protein repulsive surface
Journal Journal of Materials Science: Materials in Medicine. 2015.
Authors Jana Musilkova, Ilya Kotelnikov, Katarina Novotna, Ognen Pop-Georgievski, Frantisek Rypacek, Lucie Bacakova, Vladimir Proks
Title Hypoxia-inducible factor 3-alpha expression is associated with the stable chondrocyte phenotype
Journal Journal of Orthopaedic Research. 2015.
Authors Brandon D. Markway, Holly Cho, Jevgenia Zilberman-Rudenko, Paul Holden, Audrey McAlinden and Brian Johnstone
Title Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion
Journal Nature Medicine. 2015.
Authors Caroline Bonner, Julie Kerr-Conte, Valéry Gmyr, Gurvan Queniat, Ericka Moerman, Julien Thévenet, Cédric Beaucamps, Nathalie Delalleau, Iuliana Popescu, Willy J Malaisse, Abdullah Sener, Benoit Deprez, Amar Abderrahmani, Bart Staels & François Pattou
Title Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum free embryoid bodies.
Journal American Journal of Physiology - Cell Physiology. 2014.
Authors Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Pre, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle.
Title Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks.
Journal Neuropeptides. 2014.
Authors Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline.
Title Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks.
Journal Neuropeptides. 2014.
Authors Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline.
Title Immunoresolving actions of oral resolvin D1 include selective regulation of the transcription machinery in resolution-phase mouse macrophages.
Journal FASEB Journal. 2014.
Authors Recchiuti A, Codagnone M, Pierdomenico AM, Rossi C, Mari VC, Cianci E, Simiele F, Gatta V, Romano M.
Title Differentially Expressed MicroRNAs in Chondrocytes from Distinct Regions of Developing Human Cartilage.
Journal PLoS One. 2013.
Authors McAlinden A, Varghese N, Wirthlin L, Chang LW.

Total RNA Purification Micro Kit

This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications.

This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. # Name Elution Volume Plasma/Serum Urine Cell-Culture Media
55000 Plasma/Serum RNA Purification Mini Kit 10 - 25 µL 50 µL - 1 mL 250 µL - 1 mL 5 - 10 mL
35300 Total RNA Purification Micro Kit 20 - 50 µL 1 - 4 mL 2 - 10 mL 10 - 20 mL
17200 Total RNA Purification Kit 50 - 100 µL 4 - 10 mL 11 - 30 mL 20 - 35 mL

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 35300
Kit Specifications
Maximum Column Binding Capacity
35 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume
20 μL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)

Maximum Amount of Starting Material:

Animal Cells

Animal Tissues (Brain, Kidney, Liver, Lung, Spleen)

Heart, muscle 

Laser-Captured Microdissection (LCM)



5 x 105 cells

3 mg (for most tissues*)


up to 7.5 mL

Up to 5 x 105 cells

Time to Complete 10 Purifications
20 minutes
Average Yield
HeLa Cells (1 x 105 cells)
 
~1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 35350
Kit Specifications
Maximum Column Binding Capacity
35 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume
20 μL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)

Maximum Amount of Starting Material:
Animal Cells
Animal Tissues (Brain, Kidney, Liver, Lung, Spleen)

Heart, muscle 
Laser-Captured Microdissection (LCM)

5 x 105 cells
 

3 mg (for most tissues*)
up to 7.5 mL
Up to 5 x 105 cells
Time to Complete 10 Purifications
20 minutes
Average Yield
HeLa Cells (1 x 105 cells)
 
~1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Micro (35300, 35350)

Title Molecular diagnosis of SARS-CoV-2 in seminal fluid
Journal Journal of Endocrinological Investigation. 2021.
Authors D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo
Title Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications
Journal Journal of Biomedical Materials Research. 2017.
Authors Blanquer A, Musilkova J, Barrios L, Ibáñez E, Vandrovcova M, Pellicer E, Sort J, Bacakova L, Nogués C
Title Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome
Journal Journal of Clinical Investigation. 2016.
Authors Burnett LC, LeDuc CA, Sulsona CR, Paull D, Rausch R, Eddiry S, Carli JF, Morabito MV, Skowronski AA, Hubner G, Zimmer M, Wang L, Day R, Levy B, Fennoy I, Dubern B, Poitou C, Clement K, Butler MG, Rosenbaum M, Salles JP, Tauber M, Driscoll DJ, Egli D, Leibel RL
Title Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies
Journal International Journal of Molecular Sciences. 2016.
Authors Ma Y, Luk A, Young FP, Lynch D, Chua W, Balakrishnar B, de Souza P, Becker TM
Title Tumor necrosis factor / sphingosine-1-phosphate signaling augments resistance artery myogenic tone in diabetes
Journal Diabetes. 2016.
Authors Sauvé M, Hui SK, Dinh DD, Foltz WD, Momen A, Nedospasov SA, Offermanns S, Husain M, Kroetsch JT, Lidington D, Bolz SS
Title The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering
Journal Int J Nanomedicine. 2016.
Authors Bacakova, M., Musilkova, J., Riedel, T., Stranska, D., Brynda, E., Zaloudkova, M., & Bacakova, L
Title Sphingosine-1-Phosphate Signaling Regulates Myogenic Responsiveness in Human Resistance Arteries
Journal PLOS ONE. 2015.
Authors Sonya Hui, Andrew S. Levy, Daniel L. Slack, Marcus J. Burnstein, Lee Errett, Daniel Bonneau, David Latter, Ori D. Rotstein, Steffen-Sebastian Bolz , Darcy Lidington, Julia Voigtlaender-Bolz
Title Scleraxis is required for the development of a functional tendon enthesis
Journal FASEB Journal. 2015.
Authors Killian ML, Thomopoulos S
Title Cell adhesion and growth enabled by biomimetic oligopeptide modification of a polydopamine-poly(ethylene oxide) protein repulsive surface
Journal Journal of Materials Science: Materials in Medicine. 2015.
Authors Jana Musilkova, Ilya Kotelnikov, Katarina Novotna, Ognen Pop-Georgievski, Frantisek Rypacek, Lucie Bacakova, Vladimir Proks
Title Hypoxia-inducible factor 3-alpha expression is associated with the stable chondrocyte phenotype
Journal Journal of Orthopaedic Research. 2015.
Authors Brandon D. Markway, Holly Cho, Jevgenia Zilberman-Rudenko, Paul Holden, Audrey McAlinden and Brian Johnstone
Title Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion
Journal Nature Medicine. 2015.
Authors Caroline Bonner, Julie Kerr-Conte, Valéry Gmyr, Gurvan Queniat, Ericka Moerman, Julien Thévenet, Cédric Beaucamps, Nathalie Delalleau, Iuliana Popescu, Willy J Malaisse, Abdullah Sener, Benoit Deprez, Amar Abderrahmani, Bart Staels & François Pattou
Title Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum free embryoid bodies.
Journal American Journal of Physiology - Cell Physiology. 2014.
Authors Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Pre, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle.
Title Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks.
Journal Neuropeptides. 2014.
Authors Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline.
Title Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks.
Journal Neuropeptides. 2014.
Authors Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline.
Title Immunoresolving actions of oral resolvin D1 include selective regulation of the transcription machinery in resolution-phase mouse macrophages.
Journal FASEB Journal. 2014.
Authors Recchiuti A, Codagnone M, Pierdomenico AM, Rossi C, Mari VC, Cianci E, Simiele F, Gatta V, Romano M.
Title Differentially Expressed MicroRNAs in Chondrocytes from Distinct Regions of Developing Human Cartilage.
Journal PLoS One. 2013.
Authors McAlinden A, Varghese N, Wirthlin L, Chang LW.

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