Total RNA Purification Micro Kit
Rapid purification of total RNA - including microRNA - from small input amounts
For research use only and NOT intended for in vitro diagnostics.
Total RNA Purification Micro Kit
Rapid purification of total RNA - including microRNA - from small input amounts
Overview
- Extract high quality & quantity total RNA including miRNA
- No phenol step required - isolate all RNA in one fraction
- Elute extracted total RNA in small volume (20 µL)
- Bind & elute all RNA irrespective of size or GC content, without bias.
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications.
This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
---|---|---|---|---|---|
55000 | Plasma/Serum RNA Purification Mini Kit | 10 - 25 µL | 50 µL - 1 mL | 250 µL - 1 mL | 5 - 10 mL |
35300 | Total RNA Purification Micro Kit | 20 - 50 µL | 1 - 4 mL | 2 - 10 mL | 10 - 20 mL |
17200 | Total RNA Purification Kit | 50 - 100 µL | 4 - 10 mL | 11 - 30 mL | 20 - 35 mL |
Details
Supporting Data
Kit Specifications
|
|
Maximum Column Binding Capacity
|
35 μg
|
Maximum Column Loading Volume
|
650 μL
|
Minimum Elution Volume |
20 μL
|
Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) |
5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells |
Time to Complete 10 Purifications |
20 minutes
|
Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 35300 (50 preps) | Cat. 35350 (250 preps) |
---|---|---|
Buffer RL | 40 mL | 5 x 40 mL |
Wash Solution A | 38 mL | 5 x 38 mL |
Elution Solution A | 6 mL | 5 x 6 mL |
Micro Spin Columns | 50 | 250 |
Collection Tubes | 50 | 250 |
Elution Tubes (1.7 mL) | 50 | 250 |
Product Insert | 1 | 1 |