Rapid purification of total RNA -including microRNA - from small input amounts

  • Extract high quality & purity total RNA including miRNA
  • No phenol step required - isolate all RNA in one fraction
  • Elute extracted total RNA in small volume (20 µL)
  • Bind & elute all RNA irrespective of size or GC content, without bias.
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens

ItemsCat. #Size
Total RNA Purification Micro Kit 35300 50 preps
Total RNA Purification Micro Kit (250) 35350 250 Preps

Total RNA Purification Micro Kit

This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications.

This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Kit Specifications
Maximum Column Binding Capacity
35 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume
20 μL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)
Maximum amount of starting material:
Animal Cells
Animal Tissues
Laser-Captured Microdissection (LCM)
5 x 105 cells
3 mg (for most tissues*)
Up to 5 x 105 cells
Time to Complete 10 Purifications
20 minutes
Average Yields
HeLa Cells (1 x 105 cells)
1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 35300 (50 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Component Cat. 35350 (250 preps)
Buffer RL 5 x 40 mL
Wash Solution A 5 x 38 mL
Elution Solution A 5 x 6 mL
Micro Spin Columns 250
Collection Tubes 250
Elution Tubes (1.7 mL) 250
Product Insert 1

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