Urine Exosome Purification Kit

Features and Benefits

Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 250 µL to 30 mL
- Urine Exosome Purification Mini Kit (250 µL - 1 mL urine)
- Urine Exosome Purification Midi Kit (2 mL - 10 mL urine)
- Urine Exosome Purification Maxi Kit (11 mL - 30 mL urine)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.

The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis

Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.

Details

Supporting Data

Figure 1. Intact exosomes purified from 5 mL using different purification methods. Intact exosomes were purified from 5 mL urine using Norgen's Urine Exosome Purification Midi Kit (Cat# 57800), Competitor S's kit, and Competitor L's kit. Exosomes purified using Norgen's kit were resuspended in 400 µL Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit isolated 115 nm exosomes with a recovery of 8.36 x 10⁹ particles/mL urine samples. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification Midi Kit. Additionally, Exosomes with a broader size range covering from 75nm - 250nm were purified from 5 mL urine with a higher concentration as compared to the other two methods.

Figure 2. Intact Exosomes were purified from 10 mL urine using Norgen's Urine Exosome Purification Midi Kit (Cat# 57800) and Ultracentrifugation. Exosomes purified using Norgen's kit and ultracentrifugation were resuspended in 400 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit purified exosomes with sizes ranging from 65 nm to 195 nm, with a total recovery of 7.63 x 10⁸ particles/mL. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification Midi Kit as opposed to the exosomes purified using ultracentrifugation, which purified exosomes with larger particle sizes ranging from 125 nm - 235 nm with a total recovery of 1.56 x 10⁸ particles/mL.

Kit Specifications
Urine Input (Cat. 57700)	250 μL - 1 mL
Urine Input (Cat. 57800)	2 mL - 10 mL
Urine Input (Cat. 57900)	11 mL - 30 mL
Size of Exosomes Purified	40 nm - 150 nm
Elution Volume	Variable depending on the urine input volume
Time to Complete 10 Purifications	15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component	Cat. 57700 (50 preps)	Cat. 57800 (25 preps)	Cat. 57900 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50	25	15
Product Insert	1	1	1

Documentation

PROTOCOLS

(57700) Urine Exosome Purification Kits - Protocol (50 prep)

(57800) Urine Exosome Purification Kits - Protocol (25 prep)

(57900) Urine Exosome Purification Kits - Protocol (15 prep

SAFETY DATA SHEETS

57700 - Urine Exosome Purification Mini Kit - SDS

57800 - Urine Exosome Purification Midi Kit - SDS

57900 - Urine Exosome Purification Maxi Kit - SDS

FLYERS

Flyer

RELATED BLOGS

Videos

Urine Exosome Purification Midi Kit Tutorial (Cat. 57800)

Urine Exosome Purification Maxi Kit Tutorial (Cat. 57900)

Citations

Title	Coffee-Derived Exosome-Like Nanoparticles: Are They the Secret Heroes?
Citation	The Turkish Journal of Gastroenterology 2023.
Authors	Murat Kantarcioglu, Gülsen Yildirim, Pinar Akpinar Oktar, Serpil Yanbakan, Zeynep Büsra Özer, Deniz Yurtsever Sarica, Serpil Tasdelen, Emel Bayrak, Dilara Fatma Akin Bali, Seçkin Öztürk, Kamil Can Akçali, Üstün Ezer, and Ahmet Emin Kürekçi