Urine Exosome Purification Kit

Cat. 57700, 57800, 57900
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Urine Exosome Purification Kit

Cat. 57700, 57800, 57900

All-in-one system for the purification of urinary exosomes from different urine sample volumes

  • Purification and enrichment of intact urinary exosomes for functional studies
  • Versatile sample input ranging from 250 µL to 30 mL
    • Urine Exosome Purification Mini Kit (250 µL - 1 mL urine)
    • Urine Exosome Purification Midi Kit (2 mL - 10 mL urine)
    • Urine Exosome Purification Maxi Kit (11 mL - 30 mL urine)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • No protease treatment required
  • Compatible with urine from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Exosome Purification Mini Kit
(Cat. 57700)
50 preps
Urine Exosome Purification Midi Kit
(Cat. 57800)
25 preps
Urine Exosome Purification Maxi Kit
(Cat. 57900)
15 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

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  • Purification and enrichment of intact urinary exosomes for functional studies
  • Versatile sample input ranging from 250 µL to 30 mL
    • Urine Exosome Purification Mini Kit (250 µL - 1 mL urine)
    • Urine Exosome Purification Midi Kit (2 mL - 10 mL urine)
    • Urine Exosome Purification Maxi Kit (11 mL - 30 mL urine)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • No protease treatment required
  • Compatible with urine from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Related Services

Urine Exosome Purification Kit

The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis
Exosomes - Nanosight

Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.

Urine Exosome Purification Tutorial

Urine Exosome Purification Midi Kit Tutorial (Cat. 57800)

Kit Specifications

You have selected: Cat. 57700
Kit Specifications
Urine Input (Cat. 57700)
250 μL - 1 mL
Urine Input (Cat. 57800)
2 mL - 10 mL
Urine Input (Cat. 57900) 11 mL - 30 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume Variable depending on the
urine input volume
Time to Complete 10 Purifications
15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 57800
Kit Specifications
Urine Input (Cat. 57700)
250 μL - 1 mL
Urine Input (Cat. 57800)
2 mL - 10 mL
Urine Input (Cat. 57900) 11 mL - 30 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume Variable depending on the
urine input volume
Time to Complete 10 Purifications
15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 57900
Kit Specifications
Urine Input (Cat. 57700)
250 μL - 1 mL
Urine Input (Cat. 57800)
2 mL - 10 mL
Urine Input (Cat. 57900) 11 mL - 30 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume Variable depending on the
urine input volume
Time to Complete 10 Purifications
15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Supporting Data

Click for expanded view

Component Cat. 57700 (50 preps) Cat. 57800 (25 preps) Cat. 57900 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Mini Filter Spin Columns inserted into 2 mL tubes 50 25 15
Product Insert 1 1 1

 

Title Engineered extracellular vesicles antagonize SARS-CoV-2 infection by inhibiting mTOR signaling
Journal Biomaterials and Biosystems. 2021.
Authors A.G. Ibrahim, A. Ciullo, C.Li, G.Garcia, K. Peck, K. Miyamoto, V. Arumugaswami, E. Marbán
Title Coffee-Derived Exosome-Like Nanoparticles: Are They the Secret Heroes?
Journal LIVER. 2021.
Authors Murat Kantarcıoğlu , Gülşen Yıldırım , Pınar Akpınar Oktar , Serpil Yanbakan , Zeynep Büşra Özer , Deniz Yurtsever Sarıca ,Serpil Taşdelen, Emel Bayrak , Dilara Fatma Akın Balı , Seçkin Öztürk , Kamil Can Akçalı , Üstün Ezer ,Ahmet Emin Kürekçi
Title Profiling Tissue and Biofluid miR-155-5p, miR-155*, and miR-146a-5p Expression in Graft vs. Host Disease
Journal Frontiers in Immunology. 2021.
Authors Crossland RE, Norden J, Ghimire S, Juric MK, Pearce KF, Lendrem C, Collin M, Mischak-Weissinger E, Holler E, Greinix HT and Dickinson AM
Title Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer
Journal Diagnostics. 2021.
Authors Peng, Q.; Chiu, P.K.-F.; Wong, C.Y.-P.; Cheng, C.K.-L.; Teoh, J.Y.-C.; Ng, C.-F
Title A Urine Exosome Gene Expression Panel Distinguishes Between Indolent and Aggressive Prostate Cancers at Biopsy
Journal The Journal of Urology. 2020.
Authors Kohaar, I., Chen, Y., Banerjee, S., Borbiev, T., Kuo, H.-C., Ali, A., Kagan, J., Srivastava, S., Dobi, A., Sesterhenn, I. A., Rosner, I. L., Cullen, J., Srivastava, S., & Petrovics, G.

Urine Exosome Purification Kit

The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis
Exosomes - Nanosight

Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.

Urine Exosome Purification Tutorial

Urine Exosome Purification Midi Kit Tutorial (Cat. 57800)

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 57700
Kit Specifications
Urine Input (Cat. 57700)
250 μL - 1 mL
Urine Input (Cat. 57800)
2 mL - 10 mL
Urine Input (Cat. 57900) 11 mL - 30 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume Variable depending on the
urine input volume
Time to Complete 10 Purifications
15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 57800
Kit Specifications
Urine Input (Cat. 57700)
250 μL - 1 mL
Urine Input (Cat. 57800)
2 mL - 10 mL
Urine Input (Cat. 57900) 11 mL - 30 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume Variable depending on the
urine input volume
Time to Complete 10 Purifications
15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 57900
Kit Specifications
Urine Input (Cat. 57700)
250 μL - 1 mL
Urine Input (Cat. 57800)
2 mL - 10 mL
Urine Input (Cat. 57900) 11 mL - 30 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume Variable depending on the
urine input volume
Time to Complete 10 Purifications
15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component Cat. 57700 (50 preps) Cat. 57800 (25 preps) Cat. 57900 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Mini Filter Spin Columns inserted into 2 mL tubes 50 25 15
Product Insert 1 1 1

 

Title Engineered extracellular vesicles antagonize SARS-CoV-2 infection by inhibiting mTOR signaling
Journal Biomaterials and Biosystems. 2021.
Authors A.G. Ibrahim, A. Ciullo, C.Li, G.Garcia, K. Peck, K. Miyamoto, V. Arumugaswami, E. Marbán
Title Coffee-Derived Exosome-Like Nanoparticles: Are They the Secret Heroes?
Journal LIVER. 2021.
Authors Murat Kantarcıoğlu , Gülşen Yıldırım , Pınar Akpınar Oktar , Serpil Yanbakan , Zeynep Büşra Özer , Deniz Yurtsever Sarıca ,Serpil Taşdelen, Emel Bayrak , Dilara Fatma Akın Balı , Seçkin Öztürk , Kamil Can Akçalı , Üstün Ezer ,Ahmet Emin Kürekçi
Title Profiling Tissue and Biofluid miR-155-5p, miR-155*, and miR-146a-5p Expression in Graft vs. Host Disease
Journal Frontiers in Immunology. 2021.
Authors Crossland RE, Norden J, Ghimire S, Juric MK, Pearce KF, Lendrem C, Collin M, Mischak-Weissinger E, Holler E, Greinix HT and Dickinson AM
Title Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer
Journal Diagnostics. 2021.
Authors Peng, Q.; Chiu, P.K.-F.; Wong, C.Y.-P.; Cheng, C.K.-L.; Teoh, J.Y.-C.; Ng, C.-F
Title A Urine Exosome Gene Expression Panel Distinguishes Between Indolent and Aggressive Prostate Cancers at Biopsy
Journal The Journal of Urology. 2020.
Authors Kohaar, I., Chen, Y., Banerjee, S., Borbiev, T., Kuo, H.-C., Ali, A., Kagan, J., Srivastava, S., Dobi, A., Sesterhenn, I. A., Rosner, I. L., Cullen, J., Srivastava, S., & Petrovics, G.

 


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