RNA Clean-Up and Concentration Kits For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA Clean-Up and Concentration Kits For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol SKU 23600 Format Spin Column High Throughput Micro-Elute Size 50 Preps 100 Preps Quantity Overview Details Documentation Citations Overview Clean-up & concentrate total RNA (including miRNA) in minutes Clean-up & concentrate RNA from TRIzol®, TRI Reagent®, etc Clean-up RNA from contaminants including enzymes, primers, nucleotides Rapid spin-column protocol, elute in 20 µL 96-well format for high throughput processing & Micro-Elute spin column formats for 8 µL are available Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA. RNA Clean-Up and Concentration Kit (Spin Column) Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes. RNA Clean-Up and Concentration 96-Well Kit (High Throughput) Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes. RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute) Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes. Protocol Details Supporting Data Previous Figure 1. Effective Clean-Up to Produce High Quality Total RNA with Complete Size Diversity. Norgen's RNA Clean-Up and Concentration Kit effectively cleans up RNA isolated from phenol-based extractions without the loss of RNA diversity by retaining all RNA species including small RNAs. Total RNA was isolated from 5 x 108 E. coli using a competitor's phenol-based RNA extraction reagent. The resulting RNA was then purified using Norgen’s RNA Clean-Up and Concentration Kit. As controls, total RNA was extracted using both Norgen's Total RNA Purification Kit (#17200, no phenol required) and the phenol-based RNA extraction reagent only without any clean-up. Resolution of 7 µL of the 50 µL purified RNA on a 1X MOPS, 1.5% formaldehyde-agarose gel showed the RNA extracted with phenol was successfully cleaned-up by Norgen's RNA Clean-Up and Concentration Kit without the loss of small RNA species. Figure 2. Effective Clean-Up to Produce High Quality Total RNA Compatible to Bioanalyzer Analysis. Total RNA was isolated from 0.75 million HeLa cells using a competitor's phenol-based RNA extraction reagent. The resulting RNA was then purified using Norgen's RNA Clean-Up and Concentration Kit. As controls, total RNA was extracted using both Norgen's Total RNA Purification Kit (#17200, no phenol required) or the phenol-based RNA extraction reagent only without clean-up. Resolution of 1 µL of the 50 µL purified RNA on an Agilent RNA Nano 6000 Chip showed that without clean-up, the RNA sample isolated using the phenol-based RNA extraction reagent only resolved poorly on a bioanalyzer. In contrast, RNA extracted with the phenol-based RNA extraction reagent followed by clean-up by Norgen's RNA Clean-Up and Concentration Kit resolved properly on the bioanalyzer showing excellent quality. Figure 3. Effective Concentration and Detection of Low Amounts of RNA Input. Norgen's RNA Clean-Up and Concentration Kit can effectively concentration RNA inputs in the picogram range. Increasing amounts of HeLa RNA in 50 µL input volumes were concentrated to 20 µL using Norgen's RNA Clean-Up and Concentration Kit. A 10 µL aliquot of the purified RNA was then used as the template in a qRT-PCR reaction to detect the S14 gene. The amounts indicated on the graph correspond to the amount of RNA that was used as the input for the qRT-PCR reaction, demonstrating the consistent performance of the kit even in the picogram range. Figure 4. Effective Clean-Up to Produce High Quality RNA Transcripts. Norgen's RNA Clean-Up and Concentration Kit effectively cleans up RNA transcripts with high recovery. Three RNA transcripts (1-3) of different sizes (266n, 161n and 419n, respectively) were produced using Norgen's RNA Clean-Up and Concentration Kit. The resulting RNA transcripts were then purified using Norgen's RNA Clean-Up and Concentration Kit. Resolution of 7.5 µL of the 50 µL cleaned RNA (C) as well as an equal volume of the uncleaned input (I) on a 1X MOPS, 1.5% formaldehyde-agarose gel showed the RNA transcript was successfully cleaned-up by Norgen's RNA Clean-Up and Concentration Kit. Figure 5. Effective Clean-Up to Produce High Quality RNA Transcripts Compatible to Bioanalyzer Analysis. Norgen's RNA Clean-Up and Concentration Kit effectively cleans up RNA transcripts with high recovery. Three RNA transcripts (1-3) of different sizes (266n, 161n and 419n, respectively) were produced using Norgen's RNA Clean-Up and Concentration Kit. Resolution of 1 µL of the 50 µL cleaned RNA transcripts on an Agilent RNA Nano 6000 Chip showed that RNA transcripts cleaned by Norgen's RNA Clean-Up and Concentration Kit resolved properly on the bioanalyzer showing excellent quality. Figure 6. Excellent Quality of Concentrated RNA. Total RNA isolated from HeLa cells (2 µg) was concentrated to 8 µL using the RNA Clean-Up and Concentration Micro-Elute Kit. The excellent quality is indicated by the electropherogram generated using the Agilent 2100 Bioanalyzer (RIN > 9). The concentrated RNA is a true 'total RNA' as can be observed by the presence of small RNA species. Figure 7. High Recovery of Concentrated RNA. Total RNA isolated from HeLa cells (17.5 µL) was concentrated to 8 µL using the RNA Clean-Up and Concentration Micro-Elute Kit resulting in a 2-2.2 fold increase in RNA concentration, as was measured by Agilent 2100 Bioanalyzer quantification. Figure 8. Concentration of Total RNA. The indicated amounts of HeLa RNA were concentrated using the RNA Clean-Up and Concentration Micro-Elute Kit. For each input amount, a 17.5 µL volume was processed and the RNA eluted in 8 µL, resulting in a 2.2 fold concentration. Three microliters of each eluate were used in 10 µL RT reactions with the oligo-dT primer, followed by real-time PCR using 3 µL of the resulting cDNA and primers specific for the human RPS15 gene. On average, amplifications of the concentrated RNA reached threshold 1 Ct value before the input RNA samples, which was expected based on the concentration factor. The lowest input amount used (240 pg) was only amplified when concentrated (most right sample in figure). Figure 9. Concentration of miRNA. The indicated amounts of HeLa RNA were concentrated using the RNA Clean-Up and Concentration Micro-Elute Kit. Three microliters of each 8 µL eluate were used in 10 µL RT reactions with the miR-21-SLR primer, followed by real-time PCR using 3 µL of the resulting cDNA and the forward primer specific for miR-21. Figure 10. Concentration of RNA prior to Next Generation Sequencing (NGS) applications. Total RNA was purified from 200 µL of plasma collected on EDTA blood tubes using Norgen's Total RNA Purification Kit (Cat. 17200) and eluted in 50 µL of elution solution. The same RNA was also concentrated two-fold using the Micro-Elute RNA Column by eluting in 25 µL of elution solution. Five microliters of both the RNA without additional concentration and the 2X concentrated RNA were used as inputs to generate RNA libraries (using the NEBNext® Small RNA Library Prep Set for Illumina® and following manufacturer’s instructions) for small RNA NGS on the MiSeq (Illumina) platform. A) The prepared small RNA libraries were visualized on a 6% TBE polyacrylamide gel, where the library prepared with 2X concentrated RNA contained more ligated/indexed miRNA cDNA (147-160 bp) products than the library prepared using the RNA without concentration. B) The cDNA was extracted from excised gel bands and interrogated using the Agilent 2100 Bioanalyzer (High Sensitivity DNA Assay). As would be expected based on input, the small RNA library prepared with the 2X concentrated RNA sample was approximately two times more concentrated than the library prepared with RNA without prior concentration (39.8 vs 21.2 nM, respectively). Next Click for expanded view Kit Specifications (Spin Column) Maximum Column Binding Capacity 35 μg Size of RNA Purified All sizes, including small RNA (<200 nt) Maximum Amount of Starting Material 35 μg of RNA Minimum Elution Volume 20 μL Time to Complete 10 Purifications 20 minutes Average Recovery ≥ 90% Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 23600 (50 preps) Cat. 43200 (100 preps) Cat. 25100 (192 preps) Cat. 61000 (50 preps) Buffer RL 40 mL 2 x 40 mL 2 x 40 mL 40 mL Wash Solution A 38 mL 2 x 38 mL 2 x 38 mL 38 mL Elution Solution A 6 mL 2 x 6 mL 2 x 20 mL 6 mL Column Activation Solution - - - 30 mL Micro Spin Columns 50 100 - - Micro-Elute RNA Spin Columns - - - 50 96-Well Plate - - 2 - Adhesive Tape - - 4 - Collection Tubes 50 100 - 50 96-Well Collection Plate - - 2 - Elution Tubes (1.7 mL) 50 100 - 50 96-Well Elution Plate - - 2 - Product Insert 1 1 1 1 Documentation PROTOCOLS (23600) RNA Clean-Up and Concentration Kit (Spin Column)- Protocol (50 prep) (43200) RNA Clean-Up and Concentration Kit (Spin Column)- Protocol (100 prep) (25100) RNA Clean-Up and Concentration 96-Well Kit (High Throughput) - Protocol (96-well) (61000) RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute) - Protocol (50 prep) SUPPLEMENTARY PROTOCOLS Supplementary Protocol - NGS Library Clean-Up and Concentration APPLICATION NOTES RNA Clean-Up and Concentration Kit Effect of Microrna Gc Content On RNA Purification Efficiency - Application Note Effect of microRNA GC Content on RNA Purification Efficiency SAFETY DATA SHEETS (23600, 43200) RNA Clean-Up and Concentration Kit - Safety Data Sheet (25100) RNA Clean-Up and Concentration 96-Well Kit - Safety Data Sheet (61000) RNA Clean-Up and Concentration Micro-Elute Kit - Safety Data Sheet POSTERS Effects of The Adenovirus Type 5 Tripartite Leader Sequence With Partial and Complete Exons - ASM 2008 High Expression Level of Hepatitis C Virus E1 and Ns3 Genes - ASM 2009 Effects of Adenovirus Superinfection On Transgene Expression - ASM 2009 FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Spin Column, High Throughput (23600, 43200, 25100) Title Two-Month Voluntary Ethanol Consumption Promotes Mild Neuroinflammation in the Cerebellum but Not in the Prefrontal Cortex, Hippocampus, or Striatum of Mice Journal International Journal of Molecular Sciences. 2024. Authors Pablo Berríos-Cárcamo, Sarah Núñez, Justine Castañeda, Javiera Gallardo, María Rosa Bono, and Fernando Ezquer Title MicroRNA profiling of paediatric AML with FLT-ITD or MLL-rearrangements: Expression signatures and in vitro modulation of miR-221-3p and miR-222-3p with BRD4/HATs inhibitors Journal Oncology Reports. 2022. Authors Pier Paolo Leoncini Patrizia Vitullo Sofia Reddel Valeria Tocco Valeria Paganelli Francesca Stocchi Elena Mariggiò Michele Massa Giovanni Nigita Dario Veneziano Paolo Fadda Mario Scarpa Martina Pigazzi Alice Bertaina Rossella Rota Daria Pagliara Pietro Merli Title Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes Journal Nature Communications. 2022. Authors Norman L. Lehman, Nathalie Spassky, Müge Sak, Amy Webb, Cory T. Zumbar, Aisulu Usubalieva, Khaled J. Alkhateeb, Joseph P. McElroy, Kirsteen H. Maclean, Paolo Fadda, Tom Liu, Vineela Gangalapudi, Jamie Carver, Zied Abdullaev, Cynthia Timmers,et al. Title Novel role for tumor suppressor gene, liver kinase B1, in epithelial–mesenchymal transition leading to chronic lung allograft dysfunction Journal Am J Transplant. 2021. Authors Mohammad Rahman, Ranjithkumar Ravichandran, Sandhya Bansal, Kristina Sanborn, Sara Bowen, Jennifer Eschbacher, Angara Sureshbabu, Timothy Fleming, Ankit Bharat, Rajat Walia, Ramsey Hachem, Ross M. Bremner, Michael A. Smith, Thalachallour Mohanakumar Title Effect of adenovirus infection on transgene expression under the adenoviral MLP/TPL and the CMVie promoter/enhancer in CHO cells Journal Journal of Genetic Engineering and Biotechnology. 2017. Authors Mohamed A. El-Mogy, Moemen A.K. Abdalla, Vanja Misic, Yousef Haj-Ahmad Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma Journal The Journal of Molecular Diagnostics. 2017. Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano Title Renal Fibrosis mRNA Classifier: Validation in Experimental Lithium-Induced Interstitial Fibrosis in the Rat Kidney Journal PLOS One. 2016. Authors Marti HP, Jeffs A, Scherer A, Leader J, Leader C, Bedford J, Walker R. Title The double-domain cytidine deaminase APOBEC3G is a cellular site-specific RNA editing enzyme Journal Science Reports. 2016. Authors Sharma S, Patnaik SK, Taggart RT, Baysal BE Title Circulating miRNAs in Murine Experimental Endometriosis Decreased Abundance of let-7a Journal Reproductive Sciences. 2016. Authors Seifer, B. J., Su, D., & Taylor, H. S Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR Journal Oncology Letters. 2016. Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA Title The RNA-binding protein LARP1 is a post-transcriptional regulator of survival and tumorigenesis in ovarian cancer Journal Nucleic Acids Research. 2015. Authors Hopkins TG, Mura M, Al-Ashtal HA, Lahr RM, Abd-Latip N, Sweeney K, Lu H, Weir J, El-Bahrawy M, Steel JH, Ghaem-Maghami S, Aboagye EO, Berman AJ, Blagden SP Title Synthetically modified mRNA for efficient and fast human iPS cell generation and direct transdifferentiation to myoblasts Journal Biochemical and Biophysical Research Communications. 2015. Authors David Preskey, Thomas F. Allison, Mark Jones, Kamel Mamchaou, and Christian Unger Title A differentially expressed set of micrornas in cerebro-spinal fluid (CSF) can diagnose CNS malignancies Journal Oncotarget. 2015. Authors Alessandra Drusco, Arianna Bottoni, Alessandro Lagana, Mario Acunzo, Matteo Fassan, Luciano Cascione, Anna Antenucci, Prasanthi Kumchala, Caterina Vicentini, Marina P Gardinam, Hansjuerg Alder, Mariantonia A Carosi, Mario Ammirati, Stefano Gherardi, Marilena Luscri, Carmine Carapella, Nicola Zanesi, Carlo M Croce Title Blocking of targeted microRNAs from next-generation sequencing libraries Journal Nucleic Acids Research. 2015. Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers Title Expression of miRNAs in Bull Spermatozoa Correlates with Fertility Rates. Journal Reproduction in Domestic Animals. 2015. Authors Fagerlind M, Stålhammar H, Olsson B, Klinga-Levan K Title The Streptococcus pyogenes Orphan Protein Tyrosine Phosphatase, SP-PTP, Possesses Dual Specificity, and Essential Virulence Regulatory Functions Journal Molecular Microbiology. 2015. Authors Sashi Kant, Shivani Agarwal, Preeti Pancholi and Vijay Pancholi Title Tracking the Elusive Function of Bacillus subtilis Hfq Journal PLOS ONE. 2015. Authors Tatiana Rochat, Olivier Delumeau, Nara Figueroa-Bossi, Philippe Noirot, Lionello Bossi, Etienne Dervyn, Philippe Bouloc Title A Multi-Omics Approach Identifies Key Hubs Associated with Cell Type-Specific Responses of Airway Epithelial Cells to Staphylococcal Alpha-Toxin Journal PLOS ONE. 2015. Authors Erik Richter, Manuela Harms, Katharina Ventz, Philipp Gierok, Ravi Kumar Chilukoti, Jan-Peter Hildebrandt, Jörg Mostertz, Falko Hochgräfe Title Atrial fibrillation and rapid acute pacing regulate adipocyte/adipositas-related gene expression in the atria Journal International Journal of Cardiology. 2015. Authors R.K. Chilukoti, A. Giese, W. Malenke, G. Homuth, A. Bukowska, A., Goette, S.B. Felix, J. Kanaan, H.-G. Wollert, K. Evert, S. Verheule, P. Jais, S.N. Hatem, U. Lendeckel, C. Wolke Title The elucidation of stress memory inheritance in Brassica rapa plants Journal Frontiers in Plant Science. 2015. Authors Andriy Bilichak, Yaroslav Ilnytskyy, Rafal Wóycicki, Nina Kepeshchuk, Dawson Fogen and Igor Kovalchuk Title LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression Journal Oncogene. 2014. Authors M Mura, T G Hopkins, T Michael, N Abd-Latip, J Weir, E Aboagye, F Mauri, C Jameson, J Sturge, H Gabra, M Bushell, A E Willis, E Curry and S P Blagden Title Adipocyte-derived exosomal miRNAs: a novel mechanism for obesity-related disease Journal Pediatric Research. 2014. Authors Sarah C. Ferrante, Evan P. Nadler, Dinesh K. Pillai, Monica J. Hubal, Zuyi Wang, Justin M. Wang, Heather Gordish-Dressman, Emily Koeck, Samantha Sevilla, Andrew A. Wiles and Robert J. Freishtat Title Impact of priming on the response of neutrophils to human neutrophil alloantigen-3a antibodies. Journal Transfusion. 2014. Authors Berthold T, Muschter S, Schubert N, Wesche J, Ameling S, Teumer A, Reil A, Bux J, Bakchoul T, Greinacher A. Title RNA at 92 °C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi. Journal RNA Biology. 2013. Authors Toffano-Nioche C, Ott A, Crozat E, Nguyen AN, Zytnicki M, Leclerc F, Forterre P, Bouloc P, Gautheret D. Title Definition of the σW Regulon of Bacillus subtilis in the Absence of Stress. Journal PLOS One. 2012. Authors Zweers JC, Nicolas P, Wiegert T, van Dijl JM, Denham EL. Title Dronedarone prevents microcirculatory abnormalities in the left ventricle during atrial tachypacing. Journal British Journal of Pharmacology. 2011. Authors Bukowska A, Hammw?hner M, Sixdorf A, Schild L, Wiswedel I, R?hl FW, Wolke C, Lendeckel U, Aderkast C, Bochmann S, Chilukoti RK, Mostertz J, Bramlage P, Goette A. Title Transcriptional analysis of molecular interactions between Pseudomonas fluorescens strain TDK1, Oryza sativa and Cnaphalocrocis medinalis Journal Journal of Applied Entomology. 2010. Authors K. Saveetha, L. Karthiba, M. Raveendran, D. Saravanakumar, T. Raguchander and R. Samiyappan Title Shedding light on an extremophile lifestyle through transcriptomics Journal New Phytologist. 2009. Authors M. Dassanayake, J. S. Haas, H. J. Bohnert and J. M. Cheeseman Title Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032 Journal PLoS ONE. 2007. Authors Gioia J, Yerrapragada S, Qin X, Jiang H, Igboeli OC, Muzny D, Dugan-Rocha S, Ding Y, Hawes A, Liu W, Perez L, Kovar C, Dinh H, Lee S, Nazareth L, Blyth P, Holder M, Buhay C, Tirumalai MR, Liu Y, Dasgupta I, Bokhetache L, Fujita M, Karouia F, Eswara Moorthy P, Siefert J, Uzman A, Buzumbo P, Verma A, Zwiya H, McWilliams BD, Olowu A, Clinkenbeard KD, Newcombe D, Golebiewski L, Petrosino JF, Nicholson WL, Fox GE, Venkateswaran K, Highlander SK, Weinstock GM. Title Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile. Journal International Journal of Molecular Sciences. 2014. Authors Jessica Mühlhaus, Juliane Dinter, Daniela Nürnberg, Maren Rehders, Maren Depke, Janine Golchert, Georg Homuth, Chun-Xia Yi, Silke Morin, Josef Köhrle, Klaudia Brix, Matthias Tschöp, Gunnar Kleinau and Heike Biebermann. Title Alterations in Insulin-Signaling and Coagulation Pathways in Platelets during Hyperglycemia-Hyperinsulinemia in Healthy Non-Diabetic Subject. Journal JThrombosis Research. 2014. Authors A. Koneti Rao, Robert J. Freishtat, Gauthami Jalagadugula, Anamika Singh, Guangfen Mao, Andrew Wiles, Peter Cheung, Guenther Boden. Title Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. Journal Journal of Virology Methods. 2014. Authors Shulu Zhang, Michel Ravelonandro, Paul Russell, Nathan McOwen, Pascal Briard, Seven Bohannon, Albert Vrient. Title Analysis of Double-Stranded RNA from Microbial Communities Identifies Double-Stranded RNA Virus-like Elements. Journal Cell Reports. 2014. Authors Decker C, Parker R. Title Bacillus pumilus Reveals a Remarkably High Resistance to Hydrogen Peroxide Provoked Oxidative Stress. Journal PLoS One. 2014. Authors Handtke S, Schroeter R, Jürgen B, Methling K, Schlüter R, Albrecht D, Van Hijum S, Bongaerts J, Maurer K, Lalk M, Schweder T, Hecker M, Voigt B Title Activation of the alternative sigma factor SigB of Staphylococcus aureus following internalization by epithelial cells - An in vivo proteomics perspective. Journal International Journal of Medical Microbiology. 2013. Authors Pförtnera H, Buriana M, Michalik S, Depke M, Hildebrandt P, Dhople V, Pané-Farré J, Hecker M, Schmidt F, Völker U Title Interactions between Blood-Borne Streptococcus pneumoniae and the Blood-Brain Barrier Preceding Meningitis. Journal PLoS One. 2013. Authors Iovino F, Orihuela CJ, Moorlag HE, Molema G, Bijlsma JJ. Title Metabolic engineering of Bacillus subtilis for growth on overflow metabolites. Journal Microbial Cell Factories. 2013. Authors Kabisch J, Pratzka I, Meyer H, Albrecht D, Lalk M, Ehrenreich A, Schweder T. Title Ocular fibroblast types differ in their mRNA profiles—implications for fibrosis prevention after aqueous shunt implantation. Journal Comparative Biochemistry and Physiology. 2013. Authors Löbler M, Buß D, Kastner C, Mostertz J, Homuth G, Ernst M, Guthoff R, Wree A, Stahnke T, Fuellen G, Voelker U, Schmitz P. Title Effects of irbesartan on gene expression revealed by transcriptome analysis of left atrial tissue in a porcine model of acute rapid pacing in vivo. Journal International Journal of Cardiology. 2013. Authors Chilukoti RK, Mostertz J, Bukowska A, Aderkast C, Felix SB, Busch M, Völker U, Goette A, Wolke C, Homuth G, Lendeckel U. Title Circulating microRNA expression profiles associated with systemic lupus erythematosus. Journal Arthritis and Rheumatism. 2013. Authors Carlsen AL, Schetter AJ, Nielsen CT, Lood C, Knudsen S, Voss A, Harris CC, Hellmark T, Segelmark M, Jacobsen S, Bengtsson AA, Heegaard NH. Title Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-Interferon Activity. Journal The Journal of Biological Chemistry. 2013. Authors Harmon B, Kozina C, Maar D, Carpenter TS, Branda CS, Negrete OA, Carson BD. Title Ecological functions and differentially expressed transcripts of translucent bracts in an alpine 'glasshouse' plant Rheum nobile (Polygonaceae). Journal Planta. 2012. Authors Zhang D, Liu B, Zhao C, Lu X, Wan D, Ma F, Chen L, Liu J. Title Dronedarone prevents microcirculatory abnormalities in the left ventricle during atrial tachypacing in pigs. Journal British Journal of Pharmacology. 2012. Authors Bukowska A, Hammwöhner M, Sixdorf A, Schild L, Wiswedel I, Röhl FW, Wolke C, Lendeckel U, Aderkast C, Bochmann S, Chilukoti RK, Mostertz J, Bramlage P, Goette A. Title Temporal Profiling of Gene Networks Associated with the Late Phase of Long-Term Potentiation In Vivo. Journal PLoS ONE. 2012. Authors Ryan MM, Ryan B, Kyrke-Smith M, Logan B, Tate WP, Abraham W, Williams J. Title Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis. Journal Proceedings of the National Academy of Sciences of the United States of America. 2012. Authors Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U. Title Incorporation of Transmembrane Hydrophobic Mutations in the TCR Enhance Its Surface Expression and T Cell Functional Avidity. Journal The Journal of Immunology. 2012. Authors Haga-Friedman A, Horovitz-Fried M, J Cohen C. Title Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. Journal PLoS One. 2012. Authors Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. Title Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by the Escherichia coli signal recognition particle. Journal Molecular Biology of the Cell. 2012. Authors Welte T, Kudva R, Kuhn P, Sturm L, Braig D, Müller M, Warscheid B, Drepper F, Koch HG. Title The alternative sigma factor B modulates virulence gene expression in a murine Staphylococcus aureus infection model but does not influence kidney gene expression pattern of the host. Journal International Journal of Medicine Microbiology. 2011. Authors Depke M, Burian M, Schäfer T, Bröker BM, Ohlsen K, Völker U. Title Phenotype Enhancement Screen of a Regulatory spx Mutant Unveils a Role for the ytpQ Gene in the Control of Iron Homeostasis. Journal PLoS One. 2011. Authors Zuber P, Chauhan S, Pilaka P, Nakano M, Gurumoorthy S, Lin A, Barendt S, Chi B, Antelmann H and Mäder U. Title RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Journal Molecular Microbiology 2011. Authors Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J. Title S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics. Journal Molecular and Cellular Proteomics 2011. Authors Chi BK, Gronau K, Maeder U, Hessling B, Becher D, Antelmann H. Title Investigation of the Staphylococcus aureus GraSR Regulon Reveals Novel Links to Virulence, Stress Response and Cell Wall Signal Transduction Pathway. Journal PLoS One 2011. Authors Falord M, Mader U, Hiron A, Dbarbouille M, Msadek T. Title Characterization of the global impact of low temperature gas plasma on vegetative microorganisms. Journal Proteomics 2011. Authors Winter T, Winter J, Polak M, Kusch K, Mäder U, Sietmann R, Ehlbeck J, Hijum S, Weltmann K, Hecker M, Kusch H. Micro-Elute (61000) Title Downregulation of Talin‐1 is associated with the increased expression of miR‐182‐5p and miR‐9‐5p in coronary artery disease Journal J Clin Lab Anal. 2022 Authors Akram Gholipour, Farshad Shakerian, Ali Zahedmehr, Shiva Irani, Seyed Javad Mowla, and Mahshid Malakootian Title (Pro)renin receptor (ATP6AP2) depletion arrests As4.1 cells in the G0/G1 phase thereby increasing formation of primary cilia Journal Journal of Cellular and Molecular Medicine. 2017 Authors Wanka H, Lutze P, Staar D, Peters B, Morch A, Vogel L, Chilukoti RK, Homuth G, Sczodrok J, Bäumgen I, Peters J. Related Services NGS RNA Isolation Related Products 100b RNA Ladder 1kb RNA Ladder CleanAll DNA/RNA Clean-Up and Concentration Micro Kit RNase-Free DNase I Kit Total RNA Purification Kit
RNA Clean-Up and Concentration Kits For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA Clean-Up and Concentration Kits For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol SKU 23600 Format Spin Column High Throughput Micro-Elute Size 50 Preps 100 Preps Quantity