1kb RNA Ladder For precise sizing of a wide range of RNA molecules using native or denaturing gels For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 1kb RNA Ladder For precise sizing of a wide range of RNA molecules using native or denaturing gels Price USD$149.00 SKU 15003 Size 50 Loads Quantity Overview Details Documentation Citations Overview No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromideHighly stableNine bands from 200 bases to 4000 basesConvenient lyophilized format provides better product stability The Norgen 1 kb RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of a wide range of RNA molecules using native or denaturing agarose gels, and can be visualized under UV. Contents: • 2 vials of lyophilized RNA ladder (25 loads each)• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution• 2x loading buffer Details Supporting Data Previous Figure 1: 1 kb RNA Ladder Next Click for expanded view Specifications: • Nine discrete bands, ranging from 200 to 4,000 bases • Higher intensity band at 1,000 bases for easy reference Contents: • 2 vials of lyophilized RNA ladder (25 loads each) • 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution • 2x loading buffer Instructions: To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently. Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide. Store at -20°C. Documentation PROTOCOLS 15003 - Product Insert APPLICATION NOTES Isolation of Fractionated RNA From Hela Cells Using Norgen's Cytoplasmic Nuclear RNA Purification Kit SAFETY DATA SHEETS 1kb RNA Ladder - MSDS Citations Title Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA Journal Journal of General Plant Pathology. 2009. Authors Hataya T.
1kb RNA Ladder For precise sizing of a wide range of RNA molecules using native or denaturing gels For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 1kb RNA Ladder For precise sizing of a wide range of RNA molecules using native or denaturing gels Price USD$149.00 SKU 15003 Size 50 Loads Quantity