Biofilm DNA Isolation Kit
For the rapid preparation of genomic DNA from biofilm
For research use only and NOT intended for in vitro diagnostics.
Biofilm DNA Isolation Kit
For the rapid preparation of genomic DNA from biofilm
Overview
- Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
- No phenol or chloroform extractions
- Yields high quality DNA that is ready for PCR and other downstream applications
Norgen’s Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.
Details
Supporting Data
Kit Specifications
|
|
Maximum Column Binding Capacity |
50 µg
|
Maximum Column Loading Volume |
650 µL
|
Minimum Biofilm Input (wet weight) | 50 mg |
Maximum Biofilm Input (wet weight) |
200 mg
|
Average Yield from 200 mg of biofilm* |
2-4 µg
|
Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 62300 (50 preps) |
---|---|
Lysis Buffer L | 30 mL |
Lysis Additive A | 6 mL |
Binding Buffer I | 7 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 8 mL |
Bead Tubes | 50 |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Documentation
Citations
Title | Candida albicans extracellular vesicles trigger type I IFN signalling via cGAS and STING |
Citation | Nature Microbiology 2023. |
Authors | Hannah Brown Harding et al and Jatin M. Vyas |