Name: Stool Nucleic Acid Collection and Preservation Tubes
Brand: Norgen Biotek Corp.
SKU: 45660
Price: 735.000000 USD
Availability: InStock

Features and Benefits

Sample Homogeneity:

Eliminates sample variability.
Inactivating:

Renders the sample non-infectious by preventing bacterial/fungal growth and inactivating viruses; eliminates odor.
Ambient Temperature Preservation:

Preservation for over 2 years for DNA and 7 days for RNA at room temperature.
Cost-Effective:

No cold shipping or storage required.
Robust Preservation:

Effective over a range of temperatures.
Freezer Compatible:

The tubes can be stored at -80°C to extend the stabilization of nucleic acids for longer periods.

Applications:

DNA and RNA Isolation: Compatible with multiple isolation methods, including Norgen's Stool DNA, RNA and total nucleic acid isolation kits.

Microbiome profiling: A reliable and reproducible source of sample for microbial detection or metagenomics analysis via NGS, qPCR, ddPCR and other applications.

Metabolomics: Preserved stool samples are compatible with SCFA and metabolomic analysis according to literature.

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Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV's from fecal samples

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Details

Supporting Data

Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods.

Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples. DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).

Figure 3. Monitoring the DNA stability of Adenovirus (Ad5subE1) Spiked into Preserved Stool Samples using TaqMan Real-Time PCR Assay. Preserved stool samples were spiked with 6 x 10⁹ pfu/mL of Adenovirus, and 200 µL samples were removed at the indicated time points. The stool DNA was then isolated using Norgen's Stool DNA Isolation Kit (Cat# 27600). Next, 2 µL of DNA template from each 50 µL of elution was used in a 20 µL PCR reaction to detect the Adenovirus DNA. The stool preservative was shown to stabilize Adenovirus DNA up to 21 weeks at ambient temperature, indicating that Norgen's Stool Nucleic Acid Collection and Preservation Tubes provide a safe and convenient sample transportation system.

Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions. The microbial composition from the stool samples preserved in Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.

Figure 5. Difference In Top 5 Abundant Microorganisms at Phylum Level Compared to Fresh Stool (Day 0). Abundance of Firmicuties, Bacteroidetes and proteobacteria from the preserved stool sample was higher than non-preserved (NP) to the fresh stool after 2 days storage at RT, indicating that the originality of microorganism profile from the preserved are much closer to the fresh stool sample (Day 0).

Figure 6. Relative Abundance of Bacterial Genera Between Preserved And Non-preserved Stool Sample Stored at RT For 2 Days. Preserved stool sample using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) showed closed relativeness to the fresh stool sample (Day 0).

Figure 7. Correlation between duplicate bacterial metagenomic reads using (A) Fresh stool (day 0) and preserved stool at RT for 2 days and (B) Fresh stool (day 0) and non-preserved stool at RT for 2 days. A greater R2 was observed for Fresh stool (day 0) and preserved stool, suggestive of higher consistency between fresh stool sample and preserved stool sample

Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for 2 years after the date of shipment without any reduction in performance.

Kit Components	Cat. 45660
Stool Nucleic Acid Collection and Preservation Tubes	50
Product Insert	1

Documentation

FAQs

What does each stool collection tube contain?

Each tube contains 4 mL (~4.56 grams) of preservative. The scoop is attached to the tube cap for easily collecting and depositing the sample.

What are the dimensions of each stool collection tube?

The dimensions of each stool collection tube are 76mm (height) x 20mm (diameter).

What is the material of the stool collection tube?

Tube material is as follows:

Cap: High Density Polyethylene (HD-PE)
Stem: Polystyrene (PS)
Tube: Polypropylene (PP)

Which Norgen kits are most compatible with stool collection tubes?

Norgen's Stool DNA Isolation Kit, Stool Total RNA Purification Kit, and Stool Nucleic Acid Isolation Kit can be used with fresh stool samples or samples preserved with Stool Collection and Preservation Tubes.

Is stool preserved with stool nucleic acid preservative device compatible with metabolomics testing, such as short chain fatty acids (SCFAs) analysis, organic acid analysis, non-targeted fatty acid analysis etc.?

Yes, the preserved stool is compatible for analyses of all these metabolites. This answer is based on several independent academic research articles that used our preservation system and then analyzed the samples for various metabolites. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.

Have the Stool Nucleic Acid Collection and Preservation Tubes been tested to work with the feces of infants (birth to 6 months)?

Yes, Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.

Do the stool collection tubes also preserve microRNA?

Yes, microRNA is also preserved after stool is properly homogenized with the preservative. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications.

Can the sample preserved in stool collection tubes be frozen?

According to protocol, the tube can be store between -20°C to -70°C in order to stabilize the RNA for longer period of time. Alternatively, the end-user can aliquot the small volume of homogeneous mixture (after sample collection and mixing) from the stool tube to microcentrifuge tube and then store at -80°C. This aliquoting method also prevents the multiple freeze and thaw cycles that may impact on RNA quality.

Citations

Title	Bacteroidales markers for microbial source tracking in Southeast Asia
Citation	Water Research 2017.
Authors	Nshimyimana, J. P., Cruz, M. C., Thompson, R. J., & Wuertz, S. (2017)

Stool Nucleic Acid Collection and Preservation Tubes