ITS2 Library Preparation Kit for Illumina The reagents and components required for library preparation of the ITS2 amplicon libraries to be used for next-generation sequencing on Illumina platforms For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ITS2 Library Preparation Kit for Illumina The reagents and components required for library preparation of the ITS2 amplicon libraries to be used for next-generation sequencing on Illumina platforms Price USD$319.00 SKU 71100 Size 24 Preps 96 Preps Set A 96 Preps Set B 96 Preps Set C 96 Preps Set D Quantity Overview Details Documentation Citations Overview Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 4 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 unique dual-index libraries Sample type purification kit guide The ITS2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS2 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others. The ITS2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS2 region of the fungal DNA. The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing. Details Supporting Data Previous Figure 1. Amplicons generated by ITS2 amplicon primers, cleaned and run on a 2% agarose gel. The figure represents technical replicates of the standard DNA and No template control. Figure 2. Libraries generated by indexing PCR, cleaned and run on a 2% agarose gel. The figure represents technical replicates of the standard DNA. Figure 3. Bioanalyzer traces of ITS2 libraries generated by indexing PCR, cleaned and run on a high sensitivity DNA chip. The figure represents the library generated using standard fungal DNA. Next Click for expanded view Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL) Time to complete library preparation: 4 hours Storage Conditions and Product Stability Norgen’s ITS2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival. All kit components will remain stable for at least 1 year when stored at the specified storage conditions. Step Component Cat. 71100 (24 preps) Cat. 71110, 71120, 71130, 71140 (96 preps) Tube Cap Amplicon PCR (PCR 1) MGX Master Mix 330 μL 1320 μL ITS2 Primer Mix 70 mL 280 μL Index PCR (PCR 2) Indexing Master Mix 660 μL 2 x 1320 μL N7xx Index Primer 50 μL 50 μL S5xx Index Primer 70 μL 70 μL PCR Clean-Up Resuspension Buffer 2 x 1250 μL 2 x 5000 μL Nuclease-free Water 1250 μL 1 x 6000 μL White Documentation PROTOCOLS 71100, 71110, 71120, 71130, 71140 - Protocol Related Services NGS Related Products Bacterial Genomic DNA Isolation Kit Biofilm DNA Isolation Kit Cells and Tissue DNA Isolation Kit Fecal DNA Collection & Preservation Mini Tubes Food DNA Isolation Kit Genomic DNA Isolation Kit Microbiome DNA Isolation Kit Olive Oil DNA Isolation Kit Milk Bacterial DNA Isolation Kit Plant RNA/DNA Purification Kit Plant/Fungi DNA Isolation Kit Saliva DNA Collection, Preservation and Isolation Kit Soil DNA Isolation Kit Stool DNA Isolation Kit Stool DNA Isolation Kit (Magnetic Bead System) Stool Nucleic Acid Collection and Preservation Tubes Synovial Fluid Bacterial Genomic DNA Isolation Kit Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
ITS2 Library Preparation Kit for Illumina The reagents and components required for library preparation of the ITS2 amplicon libraries to be used for next-generation sequencing on Illumina platforms For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ITS2 Library Preparation Kit for Illumina The reagents and components required for library preparation of the ITS2 amplicon libraries to be used for next-generation sequencing on Illumina platforms Price USD$319.00 SKU 71100 Size 24 Preps 96 Preps Set A 96 Preps Set B 96 Preps Set C 96 Preps Set D Quantity
