Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples for in vitro diagnostic use Note: This product is not available for sale in the United States Powered by Bioz Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples for in vitro diagnostic use Price USD$615.00 SKU Dx42800 Format Slurry Size 50 Preps Quantity Overview Details Documentation Citations Overview Isolate all sizes of circulating and exosomal RNA, including microRNA Versatile plasma and serum input volumes (0.25 mL to 5 mL) Concentrate circulating and exosomal RNA into small elution volumes Isolate inhibitor-free circulating RNA Bind and elute all RNA irrespective of size or GC content, without bias Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Compatible with Streck Cell-Free RNA BCT® Tubes Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma/serum samples for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation. Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay. Details Supporting Data Previous Figure 1. Isolation and Detection of Circulating RNA from 1 mL Samples of Serum and Plasma Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) was used to isolate circulating RNA from 1 mL samples of serum and plasma. Five microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene and the miR-21 gene. Both the 5S housekeeping gene and the miR-21 gene could be detected from plasma (red) and serum (green), indicating the high quality of the purified RNA. The black line corresponds to the no template control. Figure 2. Effective and Consistent Detection of Plasma Exosome RNA. Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kits can effectively isolate RNA from plasma. Plasma Exosome RNA was isolated from 500 µL of human plasma prepared from blood collected on citrate, EDTA or Heparin in triplicates using Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kit Dx (blue), a ExoQuick Exosome Precipitation Reagent (green) and Qiagen’s QIAzol extraction followed by a modified Qiagen’s RNeasy Mini Kit cleanup (red). Stem loop RT-qPCR using primers specific to miR-21 and miR-16 as well as the housekeeping 5S rRNA was performed. In brief, three microliters of the 100 µL isolated RNA was then subjected to a 20 µL reverse transcription using 5S rRNA, miR-21 and miR-16 stem-loop reverse primer or reveres primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21, human miR-16 and the 5S rRNA. Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kit Dx is the only product that showed consistent detection of all tested transcripts with the highest quality regardless the type of the anti-coagulant used for blood collection. Next Click for expanded view Kit Specifications Minimum Plasma/Serum Input 0.25 mL Maximum Plasma/Serum Input 5 mL Size of RNA Purified All sizes, including microRNA Time to Complete 10 Purifications < 40 minutes Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed. Component Cat. Dx42800 (50 preps) PS Solution A 12 mL PS Solution B 100 mL PS Solution C 18 mL Wash Solution 22 mL RNA Elution Solution 6 mL Mini Filter Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (Dx42800) Plasma/Serum Circulating RNA and Exosomal Purification Kit Dx (Slurry Format) - Protocol (50 preps) SUPPLEMENTARY PROTOCOLS Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) - Supplementary Protocol PRODUCT INFORMATION SHEETS Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) - Product Information Sheet APPLICATION NOTES Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) Effect of Microrna Gc Content On RNA Purification Efficiency - Application Note SAFETY DATA SHEETS (Dx42800) Plasma/Serum Circulating RNA and Exosomal Purification Kit Dx (Slurry Format) - Safety Data Sheet RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Title A lung targeted miR-29 mimic as a therapy for pulmonary fibrosis Journal EBioMedicine. 2022. Authors Maurizio Chioccioli, Subhadeep Roy, Rachel Newell, Linda Pestano, Brent Dickinson, Kevin Rigby, Jose Herazo-Maya, et al. Title Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births Journal EBioMedicine. 2020. Authors Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V. Title Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles Journal Thrombosis Research. 2017. Authors Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma Journal The Journal of Molecular Diagnostics. 2017. Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano Title MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia Journal International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017. Authors Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role Journal OncoTargets and Therapy. 2016. Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E. Title Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation Journal Mediators of Inflammation. 2016. Authors Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue Title microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection Journal Scientific Reports. 2016. Authors Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR Journal Oncology Letters. 2016. Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA Title Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium Journal Journal of Extracellular Vesicles. 2015. Authors Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S. Title Data on individual PCR efficiency values as quality control for circulating miRNAs Journal Data in Brief. 2015. Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas Journal Cancer Prevention Research. 2015. Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa Title Blocking of targeted microRNAs from next-generation sequencing libraries Journal Nucleic Acids Research. 2015. Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes. Journal Modern Pathology. 2014. Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos. Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood. Journal Journal of Extracellular Vesicles. 2014. Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF. Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels. Journal Journal of the American Chemical Society. 2013. Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV. Related Services NGS RNA Isolation Related Products Blood DNA Isolation Mini Kit RNase-Free DNase I Kit Total RNA Purification 96-Well Kit Plasma/Serum Exosome Purification and RNA Isolation Mini Kit Urine Exosome Purification and RNA Isolation Mini Kit
Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples for in vitro diagnostic use Note: This product is not available for sale in the United States Powered by Bioz Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples for in vitro diagnostic use Price USD$615.00 SKU Dx42800 Format Slurry Size 50 Preps Quantity