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Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)

Cat. Dx42800
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Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)

Cat. Dx42800

For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples for in vitro diagnostic use

  • Isolate all sizes of circulating and exosomal RNA, including microRNA 
  • Versatile plasma and serum input volumes (0.25 mL to 5 mL)
  • Concentrate circulating and exosomal RNA into small elution volumes
  • Isolate inhibitor-free circulating RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Compatible with Streck Cell-Free RNA BCT® Tubes
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Note: This product is not available for sale in the United States

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Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)
(Cat. Dx42800)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. Dx42800
Kit Specifications
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete 10 Purifications 
< 40 minutes

 

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed.

Supporting Data

Click for expanded view

Title A lung targeted miR-29 mimic as a therapy for pulmonary fibrosis
Journal EBioMedicine. 2022.
Authors Maurizio Chioccioli, Subhadeep Roy, Rachel Newell, Linda Pestano, Brent Dickinson, Kevin Rigby, Jose Herazo-Maya, et al.
Title Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births
Journal EBioMedicine. 2020.
Authors Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V.
Title Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles
Journal Thrombosis Research. 2017.
Authors Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz
Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma
Journal The Journal of Molecular Diagnostics. 2017.
Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano
Title MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia
Journal International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017.
Authors Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal OncoTargets and Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation
Journal Mediators of Inflammation. 2016.
Authors Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue
Title microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection
Journal Scientific Reports. 2016.
Authors Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM
Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR
Journal Oncology Letters. 2016.
Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA
Title Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
Journal Journal of Extracellular Vesicles. 2015.
Authors Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S.
Title Data on individual PCR efficiency values as quality control for circulating miRNAs
Journal Data in Brief. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas
Journal Cancer Prevention Research. 2015.
Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes.
Journal Modern Pathology. 2014.
Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos.
Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood.
Journal Journal of Extracellular Vesicles. 2014.
Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF.
Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels.
Journal Journal of the American Chemical Society. 2013.
Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV.

Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma/serum samples for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.


 

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. Dx42800
Kit Specifications
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete 10 Purifications 
< 40 minutes

 

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed.

Title A lung targeted miR-29 mimic as a therapy for pulmonary fibrosis
Journal EBioMedicine. 2022.
Authors Maurizio Chioccioli, Subhadeep Roy, Rachel Newell, Linda Pestano, Brent Dickinson, Kevin Rigby, Jose Herazo-Maya, et al.
Title Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births
Journal EBioMedicine. 2020.
Authors Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V.
Title Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles
Journal Thrombosis Research. 2017.
Authors Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz
Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma
Journal The Journal of Molecular Diagnostics. 2017.
Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano
Title MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia
Journal International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017.
Authors Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal OncoTargets and Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation
Journal Mediators of Inflammation. 2016.
Authors Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue
Title microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection
Journal Scientific Reports. 2016.
Authors Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM
Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR
Journal Oncology Letters. 2016.
Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA
Title Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
Journal Journal of Extracellular Vesicles. 2015.
Authors Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S.
Title Data on individual PCR efficiency values as quality control for circulating miRNAs
Journal Data in Brief. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas
Journal Cancer Prevention Research. 2015.
Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes.
Journal Modern Pathology. 2014.
Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos.
Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood.
Journal Journal of Extracellular Vesicles. 2014.
Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF.
Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels.
Journal Journal of the American Chemical Society. 2013.
Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV.

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