Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) (Cat. Dx42800)
Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)
Cat. Dx42800
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Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)
Cat. Dx42800
For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples for in vitro diagnostic use
- Isolate all sizes of circulating and exosomal RNA, including microRNA
- Versatile plasma and serum input volumes (0.25 mL to 5 mL)
- Concentrate circulating and exosomal RNA into small elution volumes
- Isolate inhibitor-free circulating RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Compatible with Streck Cell-Free RNA BCT® Tubes
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Note: This product is not available for sale in the United States
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Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)
(Cat. Dx42800) 50 preps
(Cat. Dx42800) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Kit Specifications
You have selected: Cat. Dx42800
|
Kit Specifications
|
|
|
Minimum Plasma/Serum Input
|
0.25 mL
|
| Maximum Plasma/Serum Input |
5 mL
|
| Size of RNA Purified |
All sizes, including
microRNA |
| Time to Complete 10 Purifications |
< 40 minutes
|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed.
Supporting Data
Click for expanded view
| Component | Cat. Dx42800 (50 preps) |
|---|---|
| PS Solution A | 12 mL |
| PS Solution B | 100 mL |
| PS Solution C | 18 mL |
| Wash Solution | 22 mL |
| RNA Elution Solution | 6 mL |
| Mini Filter Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Supplementary Protocols:
Product Information Sheets:
Application Notes:
Safety Data Sheets:
| Title | A lung targeted miR-29 mimic as a therapy for pulmonary fibrosis |
| Journal | EBioMedicine. 2022. |
| Authors | Maurizio Chioccioli, Subhadeep Roy, Rachel Newell, Linda Pestano, Brent Dickinson, Kevin Rigby, Jose Herazo-Maya, et al. |
| Title | Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births |
| Journal | EBioMedicine. 2020. |
| Authors | Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V. |
| Title | Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles |
| Journal | Thrombosis Research. 2017. |
| Authors | Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz |
| Title | Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma |
| Journal | The Journal of Molecular Diagnostics. 2017. |
| Authors | Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano |
| Title | MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia |
| Journal | International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017. |
| Authors | Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu |
| Title | Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role |
| Journal | OncoTargets and Therapy. 2016. |
| Authors | Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E. |
| Title | Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation |
| Journal | Mediators of Inflammation. 2016. |
| Authors | Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue |
| Title | microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection |
| Journal | Scientific Reports. 2016. |
| Authors | Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM |
| Title | Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR |
| Journal | Oncology Letters. 2016. |
| Authors | Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA |
| Title | Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium |
| Journal | Journal of Extracellular Vesicles. 2015. |
| Authors | Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S. |
| Title | Data on individual PCR efficiency values as quality control for circulating miRNAs |
| Journal | Data in Brief. 2015. |
| Authors | Anna Brunet-Vega, Carles Pericay, MarÃa Elisa QuÃlez, MarÃa José RamÃrez-Lázaro, Xavier Calvet, Sergio Lario |
| Title | Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas |
| Journal | Cancer Prevention Research. 2015. |
| Authors | Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa |
| Title | Blocking of targeted microRNAs from next-generation sequencing libraries |
| Journal | Nucleic Acids Research. 2015. |
| Authors | Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers |
| Title | Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes. |
| Journal | Modern Pathology. 2014. |
| Authors | Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos. |
| Title | Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood. |
| Journal | Journal of Extracellular Vesicles. 2014. |
| Authors | Cheng L, Sharples RA, Scicluna BJ, Hill AF. |
| Title | Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels. |
| Journal | Journal of the American Chemical Society. 2013. |
| Authors | Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV. |
Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma/serum samples for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Supporting Data
Click for expanded view
Kit Specifications
You have selected: Cat. Dx42800
|
Kit Specifications
|
|
|
Minimum Plasma/Serum Input
|
0.25 mL
|
| Maximum Plasma/Serum Input |
5 mL
|
| Size of RNA Purified |
All sizes, including
microRNA |
| Time to Complete 10 Purifications |
< 40 minutes
|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. Dx42800 (50 preps) |
|---|---|
| PS Solution A | 12 mL |
| PS Solution B | 100 mL |
| PS Solution C | 18 mL |
| Wash Solution | 22 mL |
| RNA Elution Solution | 6 mL |
| Mini Filter Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Supplementary Protocols:
Product Information Sheets:
Application Notes:
Safety Data Sheets:
| Title | A lung targeted miR-29 mimic as a therapy for pulmonary fibrosis |
| Journal | EBioMedicine. 2022. |
| Authors | Maurizio Chioccioli, Subhadeep Roy, Rachel Newell, Linda Pestano, Brent Dickinson, Kevin Rigby, Jose Herazo-Maya, et al. |
| Title | Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births |
| Journal | EBioMedicine. 2020. |
| Authors | Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V. |
| Title | Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles |
| Journal | Thrombosis Research. 2017. |
| Authors | Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz |
| Title | Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma |
| Journal | The Journal of Molecular Diagnostics. 2017. |
| Authors | Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano |
| Title | MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia |
| Journal | International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017. |
| Authors | Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu |
| Title | Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role |
| Journal | OncoTargets and Therapy. 2016. |
| Authors | Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E. |
| Title | Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation |
| Journal | Mediators of Inflammation. 2016. |
| Authors | Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue |
| Title | microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection |
| Journal | Scientific Reports. 2016. |
| Authors | Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM |
| Title | Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR |
| Journal | Oncology Letters. 2016. |
| Authors | Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA |
| Title | Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium |
| Journal | Journal of Extracellular Vesicles. 2015. |
| Authors | Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S. |
| Title | Data on individual PCR efficiency values as quality control for circulating miRNAs |
| Journal | Data in Brief. 2015. |
| Authors | Anna Brunet-Vega, Carles Pericay, MarÃa Elisa QuÃlez, MarÃa José RamÃrez-Lázaro, Xavier Calvet, Sergio Lario |
| Title | Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas |
| Journal | Cancer Prevention Research. 2015. |
| Authors | Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa |
| Title | Blocking of targeted microRNAs from next-generation sequencing libraries |
| Journal | Nucleic Acids Research. 2015. |
| Authors | Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers |
| Title | Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes. |
| Journal | Modern Pathology. 2014. |
| Authors | Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos. |
| Title | Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood. |
| Journal | Journal of Extracellular Vesicles. 2014. |
| Authors | Cheng L, Sharples RA, Scicluna BJ, Hill AF. |
| Title | Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels. |
| Journal | Journal of the American Chemical Society. 2013. |
| Authors | Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV. |
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