Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes)

Figure 2.  Isolate a Diversity of RNA Species, from large mRNA to microRNA. Total RNA was isolated in duplicate from 3 mL hamster blood samples collected into Tempus™ RNA Blood Tubes using Norgen’s Preserved Blood RNA Purification Kit I and was subsequently used as the template in a RT-qPCR reaction for the detection of various sized RNA.  All sizes of RNA were successfully amplified including large RNA (Beta-Actin = green), small RNA, such as the 5S rRNA (blue) and microRNA, miR-21 (red), indicating both the size diversity of the purified RNA, as well as the high quality of the RNA.  No template control is shown in yellow.

Figure 3. High Yield of a Diversity of RNA Species.  Norgen's Preserved Blood RNA Purification Kit I (for use with Tempus™ Blood RNA Tubes) effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from equal amounts of goat blood collected in Tempus™ Blood RNA Tubes followed by RNA isolation using Norgen's Preserved Blood RNA Purification Kit I and a competitor's kit. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Left Panel) and for detecting miR-21 (Right Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen's kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Right Panel). Also, Norgen's kit successfully isolated similar amount of large RNA to that of the competitor’s kit (Lower Panel) indicating the full diversity of RNA species isolated.