Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) (Cat. 43400)50 preps
Format: Spin Column
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Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes)
Compatible with Tempus™ Blood RNA Tubes
Isolate true total RNA including siRNA and microRNA
No phenol extractions
Purify high-quality RNA in 30 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Preserved Blood RNA Purification Kit I provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering rNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.
* Tempus™ is a registered trademark of Life Technologies. Tempus™ Blood RNA Tubes are not provided.
Figure 1. Isolation of High Quality Total RNA. Total RNA was isolated in duplicate from 3 mL hamster blood samples collected into Tempus™RNA Blood Tubes using NorgensPreserved Blood RNA Purification Kit I and was subsequently analyzed using an Agilent BioAnalzyer. Panel A shows the electropherogram from the Agilent Bioanalyzer, where the y axis represents fluorescence units and the x axis represents the runtime (s). The bands of the 18S and 28S rRNA fragments are clearly visible and the RNA integrity number score is 9.0. Panel B demonstrates the gel banding pattern of the RNA species purified from hamster blood. Abundant blood transcripts such as the globin mRNAs constitute the additional bands to the two major rRNA bands.
Figure 2.Isolate a Diversity of RNA Species, from large mRNAto microRNA.Total RNA was isolated in duplicate from 3 mL hamster blood samples collected into Tempus™RNA Blood Tubes using NorgensPreserved Blood RNA Purification Kit I and was subsequently used as the template in a RT-qPCR reaction for the detection of various sized RNA. All sizes of RNA were successfully amplified including large RNA (Beta-Actin = green), small RNA, such as the 5S rRNA (blue) and microRNA, miR-21 (red), indicating both the size diversity of the purified RNA, as well as the high quality of the RNA. No template control is shown in yellow.
Figure 3. High Yield of a Diversity of RNA Species.Norgen's Preserved Blood RNA Purification Kit I (for use with Tempus™Blood RNA Tubes) effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from equal amounts of goat blood collected in Tempus™Blood RNA Tubes followed by RNA isolation using Norgen's Preserved Blood RNA Purification Kit I and a competitor's kit. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Left Panel) and for detecting miR-21 (Right Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen's kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Right Panel). Also, Norgen's kit successfully isolated similar amount of large RNA to that of the competitors kit (Lower Panel) indicating the full diversity of RNA species isolated.