Rapid isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes

  • Compatible with Tempus™ Blood RNA Tubes
  • Isolate true total RNA including siRNA and microRNA
  • No phenol extractions
  • Purify high-quality RNA in 30 minutes

ItemsCat. #Size
Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) 43400 50 preps

Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes)

Norgen’s Preserved Blood RNA Purification Kit I provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.

* Tempus™ is a registered trademark of Life Technologies. Tempus™ Blood RNA Tubes are not provided.

Kit Specifications
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Time to Complete 10 Purifications
20 minutes
Average Yield
5 - 25 μg per 3 mL
preserved human blood

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Title The LIFE Child study: a population-based perinatal and pediatric cohort in Germany
Journal European Journal of Epidemiology. 2017.
Authors Poulain T, Baber R, Vogel M, Pietzner D, Kirsten T, Jurkutat A, Hiemisch A,, Hilbert A,, Kratzsch J, Thiery J, Fuchs M, Hirsch C, Rauscher FG, Loeffler M,, Körner A, Nüchter M, Kiess W
Title Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank
Journal BMC Research Notes. 2016.
Authors Aarem J, Brunborg G, Aas KK, Harbak K, Taipale MM, Magnus P, Knudsen GP, Duale N.
Title Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies
Journal PLoS One. 2016.
Authors Meyer A, Paroni F, Günther K, Dharmadhikari G, Ahrens W, Kelm S, Maedler K
Title Post-weaning blood transcriptomic differences between Yorkshire pigs divergently selected for residual feed intake
Journal BMC Genomics. 2016.
Authors Liu H, Nguyen YT, Nettleton D, Dekkers JC, Tuggle CK
Title miRNA expression profiles and retinal blood vessel calibers are associated with short-term particulate matter air pollution exposure
Journal Environmental Research. 2016.
Authors Louwies T, Vuegen C, Panis LI, Cox B, Vrijens K, Nawrot TS, De Boever P
Title DICER1 and microRNA regulation in post-traumatic stress disorder with comorbid depression
Journal Nature Communications. 2015.
Authors Aliza P. Wingo, Lynn M. Almli, Jennifer J. Stevens, Torsten Klengel, Monica Uddin, Yujing Li, Angela C. Bustamante, Adriana Lori, Nastassja Koen, Dan J. Stein, Alicia K. Smith, Allison E. Aiello, Karestan C. Koenen, Derek E. Wildman, Sandro Galea, Bekh Bradley, Elisabeth B. Binder, Peng Jin, Greg Gibson & Kerry J. Ressler
Title Genetic variants in the DNA repair gene NEIL3 and the risk of myocardial infarction in a nested case–control study. The HUNT Study
Journal DNA Repair. 2015.
Authors Tonje Skarpengland, Lars Erik Laugsand, Imre Janszky, Luisa Luna, Bente Halvorsen, Carl G.P. Platou, Wei Wang, Lars J. Vatten, Jan Kristian Damås, Pål Aukrust, Magnar Bjørås, Bjørn O. Åsvold
Title Comparison of Whole Blood RNA Preservation Tubes and Novel Generation RNA Extraction Kits for Analysis of mRNA and MiRNA Profiles
Journal PLOS ONE. 2014.
Authors Madlen Häntzsch, Alexander Tolios, Frank Beutner, Dorothea Nagel, Joachim Thiery, Daniel Teupser, Lesca M. Holdt.