Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes Price USD$525.00 SKU 43500 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Compatible with PAXgene Blood RNA Tubes Isolate true total RNA including siRNA and microRNA No phenol extractions Purify high-quality RNA in 30 minutes Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing. * PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH. PAXgene Blood RNA Tubes are not provided. Details Supporting Data Previous Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitor’s kits, Norgen's Preserved Blood RNA Purification Kit II (for use with Paxgene Blood RNA Tubes) allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA without the use of phenol. Total RNA was isolated from preserved blood using Norgen's Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes) and a leading competitors kit. The purified RNA was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, only Norgen's kit was able to isolate the full diversity of RNA species, including all the small RNA species. Figure 2. High Yield of a Diversity of RNA Species. Norgen's Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes) effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from human blood collected in a PAXgene Blood RNA Tube followed by RNA isolation using Norgen's Preserved Blood RNA Purification Kit. II The purified RNA was then used as the template in a RT-qPCR for detecting the S15 gene (blue lines, in duplicate) and for detecting miR-21 (red lines, in duplicate). As it can be seen, Norgen's kit isolated complete range of RNA including high yields of mRNA and microRNA. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Time to Complete 10 Purifications 30 minutes Average Yield 5 - 20 μg per 2.5 mL preserved human blood Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C. Component Cat. 43500 (50 preps) NPX1 2 x 110 NPX2 40 mL NPX3 22 mL NPX4 6 mL NPX5 6 mL DNase I 1 vial Mini Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (43500) Preserved Blood RNA Purification Kit II - Protocol (50 prep) SAFETY DATA SHEETS (43500) Preserved Blood RNA Purification Kit II - Safety Data Sheet POSTERS FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Services NGS RNA Isolation Related Products Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) Preserved Blood RNA Purification Kit (for use with Norgen Blood RNA Preservative Tubes)
Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes Price USD$525.00 SKU 43500 Format Spin Column Size 50 Preps Quantity