Preserved Blood RNA Purification Kit II (for use with Paxgene Blood RNA Tubes) (Cat. 43500)50 preps
Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Price is dependent on a variety of factors including location and account type. Please sign in to your account to view more accurate pricing.
Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes)
Compatible with PAXgene Blood RNA Tubes
Isolate true total RNA including siRNA and microRNA
No phenol extractions
Purify high-quality RNA in 30 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.
* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH. PAXgeneBlood RNA Tubes are not provided.
Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors kits, Norgen's Preserved Blood RNA Purification Kit II (for use with PaxgeneBlood RNATubes) allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA without the use of phenol. Total RNA was isolated from preserved blood using Norgen's Preserved Blood RNA Purification Kit II(for use with PAXgeneBlood RNATubes) and a leading competitors kit. The purified RNA was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, only Norgen's kit was able to isolate the full diversity of RNA species, including all the small RNA species.
Figure 2. High Yield of a Diversity of RNA Species.Norgen's Preserved Blood RNA Purification Kit II (for use with PAXgeneBlood RNA Tubes) effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from human blood collected in aPAXgeneBlood RNA Tube followed by RNA isolation using Norgen's Preserved Blood RNA Purification Kit. IIThe purified RNA was then used as the template in a RT-qPCR for detecting the S15 gene (blue lines, in duplicate) and for detecting miR-21 (red lines, in duplicate). As it can be seen, Norgen's kit isolated complete range of RNA including high yields of mRNA and microRNA.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C.