Rapid isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes
| Items | Cat. # | Size | |
|---|---|---|---|
| Preserved Blood RNA Purification Kit II (for use with Paxgene Blood RNA Tubes) | 43500 | 50 preps |
Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes)
Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.
* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH. PAXgene Blood RNA Tubes are not provided.
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Kit Specifications
|
|
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Maximum Column Binding Capacity
|
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
| Size of RNA Purified |
All sizes, including
small RNA (< 200 nt) |
| Time to Complete 10 Purifications |
30 minutes
|
| Average Yield |
5 - 20 μg per 2.5 mL
preserved human blood |
Storage Conditions and Product Stability
The DNase I should be stored at -20oC. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers
| Title | The Development of Translational Biomarkers as a Tool for Improving the Understanding, Diagnosis and Treatment of Chronic Neuropathic Pain |
| Journal | Molecular Neurobiology. 2017 |
| Authors | Buckley DA, Jennings EM, Burke NN, Roche M, McInerney V, Wren JD, Finn DP, McHugh PC. |
| Title | Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies |
| Journal | PLoS One. 2016. |
| Authors | Meyer A, Paroni F, Günther K, Dharmadhikari G, Ahrens W, Kelm S, Maedler K |
Norgen Biotek is an innovative biotechnology company providing RNA, DNA and protein purification kits of exceptional quality, ease-of-use and sensitivity.
Copyright © 2019 - Norgen Biotek Corp.
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