Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes SKU 43400 Format Spin Column Size 50 Preps Contact Distributor Overview Details Documentation Citations Overview Compatible with Tempus™ Blood RNA Tubes Isolate true total RNA including siRNA and microRNA No phenol extractions Purify high-quality RNA in 30 minutes Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Preserved Blood RNA Purification Kit I provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering rNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing. * Tempus™ is a registered trademark of Life Technologies. Tempus™ Blood RNA Tubes are not provided. Details Supporting Data Previous Figure 1. Isolation of High Quality Total RNA. Total RNA was isolated in duplicate from 3 mL hamster blood samples collected into Tempus™ RNA Blood Tubes using Norgen’s Preserved Blood RNA Purification Kit I and was subsequently analyzed using an Agilent BioAnalzyer. Panel A shows the electropherogram from the Agilent Bioanalyzer, where the y axis represents fluorescence units and the x axis represents the runtime (s). The bands of the 18S and 28S rRNA fragments are clearly visible and the RNA integrity number score is 9.0. Panel B demonstrates the gel banding pattern of the RNA species purified from hamster blood. Abundant blood transcripts such as the globin mRNAs constitute the additional bands to the two major rRNA bands. Figure 2. Isolate a Diversity of RNA Species, from large mRNA to microRNA. Total RNA was isolated in duplicate from 3 mL hamster blood samples collected into Tempus™ RNA Blood Tubes using Norgen’s Preserved Blood RNA Purification Kit I and was subsequently used as the template in a RT-qPCR reaction for the detection of various sized RNA. All sizes of RNA were successfully amplified including large RNA (Beta-Actin = green), small RNA, such as the 5S rRNA (blue) and microRNA, miR-21 (red), indicating both the size diversity of the purified RNA, as well as the high quality of the RNA. No template control is shown in yellow. Figure 3. High Yield of a Diversity of RNA Species. Norgen's Preserved Blood RNA Purification Kit I (for use with Tempus™ Blood RNA Tubes) effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from equal amounts of goat blood collected in Tempus™ Blood RNA Tubes followed by RNA isolation using Norgen's Preserved Blood RNA Purification Kit I and a competitor's kit. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Left Panel) and for detecting miR-21 (Right Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen's kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Right Panel). Also, Norgen's kit successfully isolated similar amount of large RNA to that of the competitor’s kit (Lower Panel) indicating the full diversity of RNA species isolated. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Time to Complete 10 Purifications 20 - 30 minutes Average Yield 5 - 25 μg per 3 mL preserved human blood Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 43400 (50 preps) Tempus™ Blood RNA Tube Diluent 2 x 90 mL Lysis Solution 40 mL Wash Solution 22 mL Elution Solution 6 mL Mini Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (43400) Preserved Blood RNA Purification Kit I - Protocol (50 prep) SAFETY DATA SHEETS (43400) Preserved Blood RNA Purification Kit I - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Title Correlation between gene expression and MRI STIR signals in patients with chronic low back pain and Modic changes indicates immune involvement Journal Scientific Reports . 2022. 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Authors Poulain T, Baber R, Vogel M, Pietzner D, Kirsten T, Jurkutat A, Hiemisch A,, Hilbert A,, Kratzsch J, Thiery J, Fuchs M, Hirsch C, Rauscher FG, Loeffler M,, Körner A, Nüchter M, Kiess W Title Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank Journal BMC Research Notes. 2016. Authors Aarem J, Brunborg G, Aas KK, Harbak K, Taipale MM, Magnus P, Knudsen GP, Duale N. Title Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies Journal PLoS One. 2016. Authors Meyer A, Paroni F, Günther K, Dharmadhikari G, Ahrens W, Kelm S, Maedler K Title Differences in Whole Blood Gene Expression Associated with Infection Time-Course and Extent of Fetal Mortality in a Reproductive Model of Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection Journal PLOS ONE. 2016. Authors Wilkinson JM, Ladinig A, Bao H, Kommadath A, Stothard P, Lunney JK, Harding JC, Plastow GS Title Post-weaning blood transcriptomic differences between Yorkshire pigs divergently selected for residual feed intake Journal BMC Genomics. 2016. Authors Liu H, Nguyen YT, Nettleton D, Dekkers JC, Tuggle CK Title miRNA expression profiles and retinal blood vessel calibers are associated with short-term particulate matter air pollution exposure Journal Environmental Research. 2016. Authors Louwies T, Vuegen C, Panis LI, Cox B, Vrijens K, Nawrot TS, De Boever P Title DICER1 and microRNA regulation in post-traumatic stress disorder with comorbid depression Journal Nature Communications. 2015. Authors Aliza P. Wingo, Lynn M. Almli, Jennifer J. Stevens, Torsten Klengel, Monica Uddin, Yujing Li, Angela C. Bustamante, Adriana Lori, Nastassja Koen, Dan J. Stein, Alicia K. Smith, Allison E. Aiello, Karestan C. Koenen, Derek E. Wildman, Sandro Galea, Bekh Bradley, Elisabeth B. Binder, Peng Jin, Greg Gibson & Kerry J. Ressler Title Genetic variants in the DNA repair gene NEIL3 and the risk of myocardial infarction in a nested case–control study. The HUNT Study Journal DNA Repair. 2015. Authors Tonje Skarpengland, Lars Erik Laugsand, Imre Janszky, Luisa Luna, Bente Halvorsen, Carl G.P. Platou, Wei Wang, Lars J. Vatten, Jan Kristian Damås, Pål Aukrust, Magnar Bjørås, Bjørn O. Åsvold Title Comparison of Whole Blood RNA Preservation Tubes and Novel Generation RNA Extraction Kits for Analysis of mRNA and MiRNA Profiles Journal PLOS ONE. 2014. Authors Madlen Häntzsch, Alexander Tolios, Frank Beutner, Dorothea Nagel, Joachim Thiery, Daniel Teupser, Lesca M. Holdt. Title Anti-Interferon Beta Antibody Titers Strongly Correlate Between Two Bioassays and In Vivo Biomarker Expression, and Indicates That a Titer of 150 TRU/mL Is a Biologically Functional Cut-Point. Journal Journal of Interferon and Cytokine Research. 2014. Authors Hermanrud C, Ryner ML, Engdahl E, Fogdell-Hahn A. Related Services NGS RNA Isolation Related Products Preserved Blood RNA Purification Kit II (for use with Paxgene Blood RNA Tubes)
Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) Rapid isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes SKU 43400 Format Spin Column Size 50 Preps Contact Distributor