Urine Total RNA Purification Maxi Kit (Slurry Format) Rapid purification of total RNA from 5 mL - 10 mL urine without phenol For research use only and NOT intended for in vitro diagnostics. Diagnostic version available here Powered by Bioz Urine Total RNA Purification Maxi Kit (Slurry Format) Rapid purification of total RNA from 5 mL - 10 mL urine without phenol Price USD$545.00 SKU 29600 Format Maxi Slurry High Throughput Maxi Slurry Size Variable Quantity Overview Details Documentation Citations Overview Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA No phenol extractions Very sensitive and linear without the need for carrier RNA Bind and elute all RNA irrespective of size or GC content, without bias Isolate inhibitor-free urinary RNA Slurry/Spin column and Slurry/96-well format available resin separation matrix Purification is based on Norgen’s proprietary resin matrix Norgen’s Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA from 5 – 10 mL of urine. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies showed that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses. Details Supporting Data Previous Figure 1. Isolation and Detection of Total RNA from 10 mL Urine Samples. Norgen's Urine Total RNA Purification Maxi Kit (Slurry Format) was used to isolate total RNA from two 10 mL urine samples. The purified total RNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene (Red line) and the human 5S gene (Blue line). Five microlitres of the isolated RNA was used as the template in the RT step, and 5 µL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify both the 5S gene and the miR-21 gene in all cases, indicating the high quality of the isolated urine total RNA. The black line in the graph above corresponds to the No Template Control. Next Click for expanded view Kit Specifications Volume of Urine Processed 5 - 10 mL Size of RNA Purified All sizes, including < 200 nt Time to Complete 10 Purifications < 30 minutes Average Yield Variable depending on specimen Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 29600 (variable) Cat. 29650 (96 preps) Slurry C3 20 mL 2 x 24 mL Lysis Buffer A 2 x 130 mL 4 x 130 mL Wash Solution A 38 mL 2 x 20 mL Elution Solution A 6 mL 2 x 6 mL 96-Well Filter Plate - 1 Adhesive Tape - 1 Mini Filter Spin Columns 50 - Collection Tubes 50 - 96-Well Collection Plate - 1 Elution Tubes (1.7 mL) 50 - 96-Well Elution Plate - 1 Product Insert 1 1 Documentation PROTOCOLS (29600) Urine Total RNA Purification Maxi Kit (Slurry Format) - Protocol (variable) (29650) Urine Total RNA Purification Maxi 96-Well Kit (Slurry Format) - Protocol (96-well) APPLICATION NOTES Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input SAFETY DATA SHEETS (29600) Urine Total RNA Purification Maxi Kit (Slurry Format) - Safety Data Sheet (29650) Urine Total RNA Purification Maxi 96-Well Kit (Slurry Format) - Safety Data Sheet POSTERS Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - ASHG 2011 Differential Expression of Micrornas Assayed From Urine of Patients With Prostate Cancer Or Bph - microRNA 2012 Promising Candidate Urinary Microrna Biomarkers - TriCon 2012 RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Products Urine Exosome RNA Isolation Kit Urine microRNA Purification Kit microRNA (cel-miR-39) Spike-In Kit
Urine Total RNA Purification Maxi Kit (Slurry Format) Rapid purification of total RNA from 5 mL - 10 mL urine without phenol For research use only and NOT intended for in vitro diagnostics. Diagnostic version available here Powered by Bioz Urine Total RNA Purification Maxi Kit (Slurry Format) Rapid purification of total RNA from 5 mL - 10 mL urine without phenol Price USD$545.00 SKU 29600 Format Maxi Slurry High Throughput Maxi Slurry Size Variable Quantity