Exosome Purification

Norgen’s Exosome RNA Isolation Kits constitute an all-in-one system for the concentration and isolation of exosomal RNA from plasma, serum, ascitic fluid, urine and culture media.

There are several options:

As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids. Norgen’s Exosome RNA Isolation Kits therefore makes exosomal RNA discovery simple, rapid and reliable. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays.

Background

Exosomes are 40 - 150 nm membrane vesicles which are secreted by most cell types. Exosomes can be found in cell culture media, plasma, serum, saliva, urine, amniotic fluid and malignant ascite fluids, among other biological fluids.

Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes and other extracellular vesicles (EVs) which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNA may serve as biomarkers for various diseases including cancer. Another subset of RNA that is found in cell culture media is the free-circulating RNA (fc-RNA). These fc-RNA are usually protein-bound RNA that are leaked from cells either during apoptosis or necrosis. As the RNA molecules encapsulated within exosomes or bound to proteins (fc-RNA) are protected from degradation by RNAses, they can be efficiently recovered from cell culture media. In general, these two RNA groups contain valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status.

Ultracentrifugation, Exoquick, Filtration
Cat. # Name Elution Volume
55000 Plasma/Serum RNA Purification Mini Kit 10-25 µL
35300 Total RNA Purification Micro Kit 20-50 µL
17200 Total RNA Purification Kit 50-100 µL
Fetal Bovine Serum

An all-in-one system for the depletion of cow's exosomes from FBS prior to using it as a growth supplement in your culture medium.

Cat. # Name Size Input Volume
61100 FBS Exosome Depletion Kit I (Slurry Format) 6 preps Up to 140 mL
61400 FBS Exosome Depletion Kit II (Slurry Format) 12 preps Up to 280 mL
61200 FBS Exosome Depletion Kit I (Column Format) 6 preps Up to 140 mL
61300 FBS Exosome Depletion Kit II (Column Format) 12 preps Up to 280 mL