FFPE RNA Purification 96-Well Kit (Cat. 25400)

Norgen’s FFPE RNA Purification 96-Well Kit provides a rapid method for high throughput isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to cross linking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification 96-Well Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. Purification can be performed using either a vacuum manifold or centrifugation.