High throughput extraction and purification of RNA (including microRNA) from FFPE samples
|FFPE RNA Purification 96-Well Kit||25400||2 x 96-well plates|
FFPE RNA Purification 96-Well Kit
Norgen’s FFPE RNA Purification 96-Well Kit provides a rapid method for high throughput isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to cross linking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification 96-Well Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. Purification can be performed using either a vacuum manifold or centrifugation.
|Maximum Binding Capacity Per Well||50 μg|
|Maximum Loading Volume Per Well||500 μL|
|Size of RNA Purified||All sizes, including small RNA (< 200 nt)|
|Maximum Amount of Starting Material:
|| 5 slices of < 20 µm thick paraffin
25 mg of unsectioned block
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNase I should be stored at -20°C upon arrival. The Proteinase K should be stored in aliquots at -20oC upon reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.|
|Journal||BMC Research Notes. 2012.|
|Authors||Patnaik SK, Kannisto E, Yendamuri S.|
|Title||Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.|
|Journal||Virchows Arch. 2012.|
|Authors||Ludyga N, Grônwald B, Azimzadeh O, Englert S, H?fler H, Tapio S, Aubele M.|
|Title||Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells.|
|Journal||PLoS One. 2011.|
|Authors||Patnaik S, Kannisto E, Mallick R, Yendamuri S.|
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