FFPE RNA Purification Kit Dx For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples for in vitro diagnostic use Note: This product is not available for sale in the United StatesResearch versions available here Powered by Bioz FFPE RNA Purification Kit Dx For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples for in vitro diagnostic use Price USD$455.00 SKU Dx25300 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview CE-IVD marked in accordance with EU Directive 98/79/EC Ideal for use in in vitro diagnostic workflows Extract total RNA (including microRNA) from FFPE samples No phenol extraction step Includes DNase for optional on-column DNA removal Isolated RNA is of the highest quality and integrity Isolate a diversity of RNA species Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s FFPE RNA Purification Kit Dx provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit Dx provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. Details Supporting Data Previous Figure 1. High Quality and Yield of Total RNA. Norgen's FFPE RNA Purification Kit Dx isolates FFPE RNA that exceeds both yield and quality of competitors. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen's FFPE RNA Purification Kit Dx and a leading competitor’s kit. One microliter of the 50 µL purified RNA was then resolved on an Agilent 2100 BioAnalzyer using an RNA Nano 6000 chip. As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen's RNA. Figure 2. Higher Yield of FFPE RNA Isolated by Norgen's FFPE RNA Purification Kit Dx. Norgen's FFPE RNA Purification Kit Dx isolates FFPE RNA that exceeds the yield of competitors. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen's FFPE RNA Purification Kit Dx and two leading competitor's kits. Eighteen isolations were performed for each product. The graph demonstrates the mean yield of RNA according to spectrophotometry for 18 sample replicates. Vertical bars represent the standard deviation. Norgen's kit consistently purified total RNA with a higher yield than for those obtained using the market competitor's kits. Figure 3. High Yield of a Diversity of RNA Species. Norgen's FFPE RNA Purification Kit Dx effectively recovers all sizes of RNA from large mRNA to small RNA including microRNAs. Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen's FFPE RNA Purification Kit Dx and a leading competitor’s kit. Five microliters of the 50 µL purified RNA was then used as the template in a 20 µL reverse transcription reaction with oligo dT primer or miR-21 stem-loop reverse primer. Two microliters of the RT reaction was then used in a 15 µL qPCR reaction for detecting the beta-actin gene (Panel A) and for detecting miR-21(Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the green lines correspond to competitor-isolated RNA. As it can be seen, Norgen's kit isolated higher yields of RNA in both cases, as indicated by the lower Ct values of the blue lines. Also, Norgen's kit successfully isolated not only large RNA (Panel A) but also microRNA (Panel B), indicating the diversity of RNA species isolated. Figure 4. Excellent Performance in Downstream Application such as RT-qPCR. Norgen's FFPE RNA Purification Kit Dx effectively recovers all sizes of RNA, from large mRNA to small RNA including microRNA, that perform effectively in sensitive applications such as RT-qPCR. Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen's FFPE RNA Purification Kit and two market competitors. The graph demonstrates the mean delta Ct value for 18 sample replicates. Vertical bars represent the standard deviation. Norgen's kit consistently isolates RNA of a higher yield and quality than that obtained by the two leading market competitors as indicated by the larger delta Ct values. Not only were the large RNA species detected including rRNA and mRNA, but small RNA species (microRNA) were also detected from the sample, indicating the diversity of RNA species isolated. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity Up to 50 µg RNA Maximum Loading Volume Per Spin Column 650 µL Size of RNA Purified All sizes, including small RNA (< 200 nt) Time to Complete 10 Purifications 1-4 hours* Maximum Amount of Starting Material 5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block Average Yield Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney * Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 2 years in their unopened containers. Component Cat. 25300 (50 preps) Digestion Buffer 20 mL Binding Solution 20 mL Enzyme Incubation Buffer 6 mL Wash Solution 22 mL Elution Solution 6 mL Proteinase K 12 mg DNase I 200 μL Micro Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (Dx25300) FFPE RNA Purification Kit Dx - Protocol (50 prep) APPLICATION NOTES Rapid Method for the Purification of Total RNA from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Samples Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input SAFETY DATA SHEETS (Dx25300) FFPE RNA Purification Kit Dx - Safety Data Sheet POSTERS FFPE RNA Purification Kit Evaluation of Different Extraction Methods For Recovering Nucleic Acids From Formalinfixed Paraffin Embedded Tissue Samples - Poster FFPE RNA Purification Kit Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - Poster RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Services NGS RNA Isolation Related Products 100b RNA Ladder FFPE RNA Purification 96-Well Kit FFPE RNA/DNA Purification Plus Kit RNase-Free DNase I Kit
FFPE RNA Purification Kit Dx For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples for in vitro diagnostic use Note: This product is not available for sale in the United StatesResearch versions available here Powered by Bioz FFPE RNA Purification Kit Dx For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples for in vitro diagnostic use Price USD$455.00 SKU Dx25300 Format Spin Column Size 50 Preps Quantity