For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples

  • Extract total RNA (including microRNA) from FFPE samples
  • No phenol extraction step
  • Includes DNase for optional on-column DNA removal
  • Isolated RNA is of the highest quality and integrity
  • Isolate a diversity of RNA species
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

Related Services

NGS Service RNA Isolation Services

ItemsCat. #Size
FFPE RNA Purification Kit 25300 50 preps

FFPE RNA Purification Kit

Norgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time.

Kit Specifications
Maximum Column Binding Capacity
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications
1-4 hours*
Maximum Amount of Starting Material
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block
Average Yield
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

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