For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples

  • Extract total RNA (including microRNA) from FFPE samples
  • No phenol extraction step
  • Includes DNase for optional on-column DNA removal
  • Isolated RNA is of the highest quality and integrity

Items Cat. # Size
FFPE RNA Purification Kit 25300 50 preps

FFPE RNA Purification Kit

Norgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The process first involves deparaffinization of the FFPE samples through a series of xylene and ethanol washes. Next, the FFPE samples are digested with the provided Proteinase K and Digestion Buffer. Binding Solution and ethanol are then added to the lysate, and the solution is loaded onto a spin-column (BIND)Under these conditions only the RNA will bind while most of the contaminants will be removed in the flowthrough. At this point, any remaining traces of genomic DNA can be digested using an optional protocol, allowing for pure RNA samples to be isolated. The bound RNA is then washed (WASH) in order to remove any impurities.  Lastly, the purified total RNA is eluted in the provided Elution Buffer or water (ELUTE).  Please see the procedure flowchart to the right. 

  • High quality and integrity of the isolated RNA - The purified total RNA is of the highest quality and integrity, and can be used in any sensitive downstream applications.
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
  • High yields - Norgen’s FFPE RNA Purification Kit allows for the purification of high yields of total RNA.
  • No phenol:chloroform extractions - Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform.
  • Rapid procedure - Isolate total RNA from FFPE tissue sections using a rapid spin column format in as little as 1 hour

Kit Specifications
Binding Capacity Per Column
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including small RNA and microRNA (< 200 nt)
Time to Complete 10 Purifications
1-4 hours*
Maximum Amount of Starting Materila
5 slices of 20 µm thick paraffin slices
25 mg of unsectioned block
Average Yield
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 25300 (50 preps)
Digestion Buffer A 25 mL
Buffer RL 30 mL
Enzyme Incubation Buffer 6 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Proteinase K 12 mg
DNase I 1 vial
Mini Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies
Journal Journal of Thoracic Oncology. 2015.
Authors Patnaik, Santosh, Mallick, Reema, Kannisto, Eric MS, Sharma, Rohit, Bshara, Wiam, Yendamuri, Sai M, Dhillon, Samjot Singh
Title LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis
Journal The Journal of Clinical Investigation. 2014.
Authors Shi-Dong Ma, Xuequn Xu, Julie Plowshay, Erik A. Ranheim, William J. Burlingham, Jeffrey L. Jensen, Fotis Asimakopoulos, Weihua Tang, Margaret L. Gulley, Ethel Cesarman, Jenny E. Gumperz and Shannon C. Kenney
Title Robust global microRNA expression profiling using next-generation sequencing technologies.
Journal Laboratory Investigation. 2014.
Authors Tam S, Borja R, Tsao M, and McPherson J.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grônwald B, Azimzadeh O, Englert S, H?fler H, Tapio S, Aubele M.
Title Simultaneous recovery of DNA and RNA from formalin-fixed paraffin-embedded tissue and application in epidemiologic studies
Journal Cancer Epidemiology, Biomarkers & Prevention. 2010.
Authors Huang WY, Sheehy TM, Moore LE, Hsing AW, Purdue MP.