FFPE RNA Purification Kits

Cat. 25300, 25400
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FFPE RNA Purification Kits

Cat. 25300, 25400

For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples

  • Extract total RNA (including microRNA) from FFPE samples
  • No phenol extraction step
  • Includes DNase for optional on-column DNA removal
  • Isolated RNA is of the highest quality and integrity
  • Isolate a diversity of RNA species
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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FFPE RNA Purification Kit
(Cat. 25300)
50 preps
FFPE RNA Purification 96-Well Kit
(Cat. 25400)
2 x 96-well plates

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Extract total RNA (including microRNA) from FFPE samples
  • No phenol extraction step
  • Includes DNase for optional on-column DNA removal
  • Isolated RNA is of the highest quality and integrity
  • Isolate a diversity of RNA species
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Related Services

FFPE RNA Purification Kits

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

FFPE RNA Purification Kit

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

FFPE RNA Purification 96-Well Kit

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Kit Specifications

You have selected: Cat. 25300
Kit Specifications
Maximum Column Binding Capacity
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications
1-4 hours*
Maximum Amount of Starting Material
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block
Average Yield
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
You have selected: Cat. 25400
Kit Specifications
Maximum Binding Capacity Per Well 50 μg
Maximum Loading Volume Per Well 400 μL
Size of RNA Purified All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:
 
 5 slices of < 20 µm thick paraffin 
25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 25300 (50 preps) Cat. 25400 (2 x 96 preps)
Digestion Buffer A 25 mL 2 x 25 mL
Buffer RL 30 mL 2 x 30 mL
Enzyme Incubation Buffer 6 mL 2 x 6 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Solution A 6 mL 2 x 20 mL
Proteinase K 12 mg 2 x 20 mg
DNase I 1 vial 2 x 500μL
Micro Spin Columns 50 -
96-Well Incubation Plate - 2
96-Well Plate - 2
Adhesive Tape - 8
Collection Tubes 50 -
96-Well Collection Plate - 2
Elution Tubes (1.7 mL) 50 -
96-Well Elution Plate - 2
Product Insert 1 1

Regular (25300)

Title Identification of Differentially Expressed Intronic Transcripts in Osteosarcoma
Journal Non-Coding RNA. 2022.
Authors Emel Rothzerg, Jiake Xu, and David Wood
Title Zona Pellucida sperm-binding protein 3 receptor distribution during Gopc−/− globozoospermic spermatogenesis
Journal Microscopy Research & Technique. 2021.
Authors Maider Bizkarguenaga,Laura Gomez-Santos,Juan Francisco Madrid,Francisco José Sáez,Edurne Alonso
Title The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues
Journal BMC Cancer. 2019.
Authors Michal Marczyk, Chunxiao Fu, Rosanna Lau, Lili Du, Alexander J. Trevarton, Bruno V. Sinn, Rebekah E. Gould, Lajos Pusztai, Christos Hatzis & W. Fraser Symmans
Title miR-17-92a-1 cluster host gene (MIR17HG) evaluation and response to neoadjuvant chemoradiotherapy in rectal cancer
Journal OncoTargets and Therapy. 2016.
Authors Molinari C, Salvi S, Foca F, Teodorani N, Saragoni L, Puccetti M, Passardi A, Tamberi S, Avanzolini A, Lucci E, Calistri D
Title Involucrin Expression and Association with Ki-67 in Paraffin Embedded Tissue of Canine Skin Tumors: A Retrospective Study
Journal The Thai Journal of Veterinary Medicine. 2016.
Authors Assawawongkasem, N., Suriyaphol, G., Srisuwatanasagul, S., Theerawattanasirikul, S., & Sailasuta, A
Title Correlative Analysis of miRNA Expression and Oncotype Dx Recurrence Score in Estrogen Receptor Positive Breast Carcinomas
Journal PLOS ONE. 2015.
Authors Rajyasree Emmadi , Emanuele Canestrari, Zarema H. Arbieva, Wenbo Mu, Yang Dai, Jonna Frasor, Elizabeth Wiley
Title Ocoxin® oral solution slows down tumor growth in an experimental model of colorectal cancer metastasis to the liver in Balb/c mice.
Journal Oncology Reports. 2015.
Authors Márquez J, Mena J, Hernandez-Unzueta I, Benedicto A, Sanz E, Arteta B, Olaso E
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Telomeric repeat-containing RNA (TERRA) constitutes a nucleoprotein component of extracellular inflammatory exosomes
Journal PNAS. 2015.
Authors Zhuo Wang, Zhong Deng, Nadia Dahmane, Kevin Tsai, Pu Wang, Dewight R. Williams, Andrew V. Kossenkov, Louise C. Showe, Rugang Zhang, Qihong Huang, José R. Conejo-Garcia, and Paul M. Lieberman
Title A phase II and pharmacodynamic study of sunitinib in relapsed/refractory oesophageal and gastro-oesophageal cancers
Journal British Journal of Cancer. 2015.
Authors C Wu, S Mikhail, L Wei, C Timmers, S Tahiri, A Neal, J Walker, S El-Dika, M Blazer, J Rock, D J Clark, X Yang, J L Chen, J Liu, M V Knopp and T Bekaii-Saab
Title MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies
Journal Journal of Thoracic Oncology. 2015.
Authors Patnaik, Santosh, Mallick, Reema, Kannisto, Eric MS, Sharma, Rohit, Bshara, Wiam, Yendamuri, Sai M, Dhillon, Samjot Singh
Title LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis
Journal The Journal of Clinical Investigation. 2014.
Authors Shi-Dong Ma, Xuequn Xu, Julie Plowshay, Erik A. Ranheim, William J. Burlingham, Jeffrey L. Jensen, Fotis Asimakopoulos, Weihua Tang, Margaret L. Gulley, Ethel Cesarman, Jenny E. Gumperz and Shannon C. Kenney
Title Robust global microRNA expression profiling using next-generation sequencing technologies.
Journal Laboratory Investigation. 2014.
Authors Tam S, Borja R, Tsao M, and McPherson J.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grônwald B, Azimzadeh O, Englert S, H?fler H, Tapio S, Aubele M.
Title Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells.
Journal PLoS One. 2011.
Authors Patnaik S, Kannisto E, Mallick R, Yendamuri S.

96-Well (25400)

Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grônwald B, Azimzadeh O, Englert S, H?fler H, Tapio S, Aubele M.
Title Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells.
Journal PLoS One. 2011.
Authors Patnaik S, Kannisto E, Mallick R, Yendamuri S.

FFPE RNA Purification Kits

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

FFPE RNA Purification Kit

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

FFPE RNA Purification 96-Well Kit

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 25300
Kit Specifications
Maximum Column Binding Capacity
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications
1-4 hours*
Maximum Amount of Starting Material
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block
Average Yield
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
You have selected: Cat. 25400
Kit Specifications
Maximum Binding Capacity Per Well 50 μg
Maximum Loading Volume Per Well 400 μL
Size of RNA Purified All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:
 
 5 slices of < 20 µm thick paraffin 
25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component Cat. 25300 (50 preps) Cat. 25400 (2 x 96 preps)
Digestion Buffer A 25 mL 2 x 25 mL
Buffer RL 30 mL 2 x 30 mL
Enzyme Incubation Buffer 6 mL 2 x 6 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Solution A 6 mL 2 x 20 mL
Proteinase K 12 mg 2 x 20 mg
DNase I 1 vial 2 x 500μL
Micro Spin Columns 50 -
96-Well Incubation Plate - 2
96-Well Plate - 2
Adhesive Tape - 8
Collection Tubes 50 -
96-Well Collection Plate - 2
Elution Tubes (1.7 mL) 50 -
96-Well Elution Plate - 2
Product Insert 1 1

Regular (25300)

Title Identification of Differentially Expressed Intronic Transcripts in Osteosarcoma
Journal Non-Coding RNA. 2022.
Authors Emel Rothzerg, Jiake Xu, and David Wood
Title Zona Pellucida sperm-binding protein 3 receptor distribution during Gopc−/− globozoospermic spermatogenesis
Journal Microscopy Research & Technique. 2021.
Authors Maider Bizkarguenaga,Laura Gomez-Santos,Juan Francisco Madrid,Francisco José Sáez,Edurne Alonso
Title The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues
Journal BMC Cancer. 2019.
Authors Michal Marczyk, Chunxiao Fu, Rosanna Lau, Lili Du, Alexander J. Trevarton, Bruno V. Sinn, Rebekah E. Gould, Lajos Pusztai, Christos Hatzis & W. Fraser Symmans
Title miR-17-92a-1 cluster host gene (MIR17HG) evaluation and response to neoadjuvant chemoradiotherapy in rectal cancer
Journal OncoTargets and Therapy. 2016.
Authors Molinari C, Salvi S, Foca F, Teodorani N, Saragoni L, Puccetti M, Passardi A, Tamberi S, Avanzolini A, Lucci E, Calistri D
Title Involucrin Expression and Association with Ki-67 in Paraffin Embedded Tissue of Canine Skin Tumors: A Retrospective Study
Journal The Thai Journal of Veterinary Medicine. 2016.
Authors Assawawongkasem, N., Suriyaphol, G., Srisuwatanasagul, S., Theerawattanasirikul, S., & Sailasuta, A
Title Correlative Analysis of miRNA Expression and Oncotype Dx Recurrence Score in Estrogen Receptor Positive Breast Carcinomas
Journal PLOS ONE. 2015.
Authors Rajyasree Emmadi , Emanuele Canestrari, Zarema H. Arbieva, Wenbo Mu, Yang Dai, Jonna Frasor, Elizabeth Wiley
Title Ocoxin® oral solution slows down tumor growth in an experimental model of colorectal cancer metastasis to the liver in Balb/c mice.
Journal Oncology Reports. 2015.
Authors Márquez J, Mena J, Hernandez-Unzueta I, Benedicto A, Sanz E, Arteta B, Olaso E
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Telomeric repeat-containing RNA (TERRA) constitutes a nucleoprotein component of extracellular inflammatory exosomes
Journal PNAS. 2015.
Authors Zhuo Wang, Zhong Deng, Nadia Dahmane, Kevin Tsai, Pu Wang, Dewight R. Williams, Andrew V. Kossenkov, Louise C. Showe, Rugang Zhang, Qihong Huang, José R. Conejo-Garcia, and Paul M. Lieberman
Title A phase II and pharmacodynamic study of sunitinib in relapsed/refractory oesophageal and gastro-oesophageal cancers
Journal British Journal of Cancer. 2015.
Authors C Wu, S Mikhail, L Wei, C Timmers, S Tahiri, A Neal, J Walker, S El-Dika, M Blazer, J Rock, D J Clark, X Yang, J L Chen, J Liu, M V Knopp and T Bekaii-Saab
Title MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies
Journal Journal of Thoracic Oncology. 2015.
Authors Patnaik, Santosh, Mallick, Reema, Kannisto, Eric MS, Sharma, Rohit, Bshara, Wiam, Yendamuri, Sai M, Dhillon, Samjot Singh
Title LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis
Journal The Journal of Clinical Investigation. 2014.
Authors Shi-Dong Ma, Xuequn Xu, Julie Plowshay, Erik A. Ranheim, William J. Burlingham, Jeffrey L. Jensen, Fotis Asimakopoulos, Weihua Tang, Margaret L. Gulley, Ethel Cesarman, Jenny E. Gumperz and Shannon C. Kenney
Title Robust global microRNA expression profiling using next-generation sequencing technologies.
Journal Laboratory Investigation. 2014.
Authors Tam S, Borja R, Tsao M, and McPherson J.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grônwald B, Azimzadeh O, Englert S, H?fler H, Tapio S, Aubele M.
Title Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells.
Journal PLoS One. 2011.
Authors Patnaik S, Kannisto E, Mallick R, Yendamuri S.

96-Well (25400)

Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grônwald B, Azimzadeh O, Englert S, H?fler H, Tapio S, Aubele M.
Title Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells.
Journal PLoS One. 2011.
Authors Patnaik S, Kannisto E, Mallick R, Yendamuri S.

 


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