FFPE RNA Purification Kits (Cat. 25300, 25400)
FFPE RNA Purification Kits
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What Scientists are Saying About this Product
The Norgen FFPE RNA Purification Kit 25300 enable our laboratory to access the RNA and transcriptomic information from the archived paraffin blocks. We have implemented this technology as part of our genomic pathology routine and it has become indispensable. This approach gives us an opportunity to increase the number of samples for rare diseases and improves the power of the studies using genomic pathology.
- Murdoch university
My customer used the [FFPE Purification] kit with microdissection samples (containing about 100 cells) and she obtained very good results with microRNA...
- Elletrofor, Italy
FFPE RNA Purification Kits
For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples
- Extract total RNA (including microRNA) from FFPE samples
- No phenol extraction step
- Includes DNase for optional on-column DNA removal
- Isolated RNA is of the highest quality and integrity
- Isolate a diversity of RNA species
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
CEIVDD marked diagnostic version available here
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(Cat. 25300) 50 preps
(Cat. 25400) 2 x 96-well plates
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Please note that Norgen Biotek provides sample kits exclusively to residents of North America. Customers outside of North America may request a sample from one of our global distributors. Find your local distributor.
Kit Specifications
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
Up to 50 µg RNA
|
| Maximum Loading Volume Per Spin Column |
650 µL
|
| Size of RNA Purified |
All sizes, including small RNA (< 200 nt)
|
| Time to Complete 10 Purifications |
1-4 hours*
|
| Maximum Amount of Starting Material |
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block |
| Average Yield |
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
|
Kit Specifications
|
|
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 400 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: |
5 slices of < 20 µm thick paraffin 25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
Supporting Data
Spin Column (25300)
| Title | Identification of Differentially Expressed Intronic Transcripts in Osteosarcoma |
| Journal | Non-Coding RNA. 2022. |
| Authors | Emel Rothzerg, Jiake Xu, and David Wood |
| Title | Zona Pellucida sperm-binding protein 3 receptor distribution during Gopc−/− globozoospermic spermatogenesis |
| Journal | Microscopy Research & Technique. 2021. |
| Authors | Maider Bizkarguenaga,Laura Gomez-Santos,Juan Francisco Madrid,Francisco José Sáez,Edurne Alonso |
| Title | The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues |
| Journal | BMC Cancer. 2019. |
| Authors | Michal Marczyk, Chunxiao Fu, Rosanna Lau, Lili Du, Alexander J. Trevarton, Bruno V. Sinn, Rebekah E. Gould, Lajos Pusztai, Christos Hatzis & W. Fraser Symmans |
| Title | miR-17-92a-1 cluster host gene (MIR17HG) evaluation and response to neoadjuvant chemoradiotherapy in rectal cancer |
| Journal | OncoTargets and Therapy. 2016. |
| Authors | Molinari C, Salvi S, Foca F, Teodorani N, Saragoni L, Puccetti M, Passardi A, Tamberi S, Avanzolini A, Lucci E, Calistri D |
| Title | Involucrin Expression and Association with Ki-67 in Paraffin Embedded Tissue of Canine Skin Tumors: A Retrospective Study |
| Journal | The Thai Journal of Veterinary Medicine. 2016. |
| Authors | Assawawongkasem, N., Suriyaphol, G., Srisuwatanasagul, S., Theerawattanasirikul, S., & Sailasuta, A |
| Title | Correlative Analysis of miRNA Expression and Oncotype Dx Recurrence Score in Estrogen Receptor Positive Breast Carcinomas |
| Journal | PLOS ONE. 2015. |
| Authors | Rajyasree Emmadi , Emanuele Canestrari, Zarema H. Arbieva, Wenbo Mu, Yang Dai, Jonna Frasor, Elizabeth Wiley |
| Title | Ocoxin® oral solution slows down tumor growth in an experimental model of colorectal cancer metastasis to the liver in Balb/c mice. |
| Journal | Oncology Reports. 2015. |
| Authors | Márquez J, Mena J, Hernandez-Unzueta I, Benedicto A, Sanz E, Arteta B, Olaso E |
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | Telomeric repeat-containing RNA (TERRA) constitutes a nucleoprotein component of extracellular inflammatory exosomes |
| Journal | PNAS. 2015. |
| Authors | Zhuo Wang, Zhong Deng, Nadia Dahmane, Kevin Tsai, Pu Wang, Dewight R. Williams, Andrew V. Kossenkov, Louise C. Showe, Rugang Zhang, Qihong Huang, José R. Conejo-Garcia, and Paul M. Lieberman |
| Title | A phase II and pharmacodynamic study of sunitinib in relapsed/refractory oesophageal and gastro-oesophageal cancers |
| Journal | British Journal of Cancer. 2015. |
| Authors | C Wu, S Mikhail, L Wei, C Timmers, S Tahiri, A Neal, J Walker, S El-Dika, M Blazer, J Rock, D J Clark, X Yang, J L Chen, J Liu, M V Knopp and T Bekaii-Saab |
| Title | MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies |
| Journal | Journal of Thoracic Oncology. 2015. |
| Authors | Patnaik, Santosh, Mallick, Reema, Kannisto, Eric MS, Sharma, Rohit, Bshara, Wiam, Yendamuri, Sai M, Dhillon, Samjot Singh |
| Title | LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis |
| Journal | The Journal of Clinical Investigation. 2014. |
| Authors | Shi-Dong Ma, Xuequn Xu, Julie Plowshay, Erik A. Ranheim, William J. Burlingham, Jeffrey L. Jensen, Fotis Asimakopoulos, Weihua Tang, Margaret L. Gulley, Ethel Cesarman, Jenny E. Gumperz and Shannon C. Kenney |
| Title | Robust global microRNA expression profiling using next-generation sequencing technologies. |
| Journal | Laboratory Investigation. 2014. |
| Authors | Tam S, Borja R, Tsao M, and McPherson J. |
| Title | Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. |
| Journal | BMC Research Notes. 2012. |
| Authors | Patnaik SK, Kannisto E, Yendamuri S. |
| Title | Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. |
| Journal | PLoS One. 2011. |
| Authors | Patnaik S, Kannisto E, Mallick R, Yendamuri S. |
High Throughput (25400)
| Title | Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. |
| Journal | BMC Research Notes. 2012. |
| Authors | Patnaik SK, Kannisto E, Yendamuri S. |
| Title | Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. |
| Journal | PLoS One. 2011. |
| Authors | Patnaik S, Kannisto E, Mallick R, Yendamuri S. |
FFPE RNA Purification Kits
Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.
FFPE RNA Purification Kit (Spin Column)
Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
FFPE RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
Up to 50 µg RNA
|
| Maximum Loading Volume Per Spin Column |
650 µL
|
| Size of RNA Purified |
All sizes, including small RNA (< 200 nt)
|
| Time to Complete 10 Purifications |
1-4 hours*
|
| Maximum Amount of Starting Material |
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block |
| Average Yield |
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
|
Kit Specifications
|
|
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 400 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: |
5 slices of < 20 µm thick paraffin 25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
Spin Column (25300)
| Title | Identification of Differentially Expressed Intronic Transcripts in Osteosarcoma |
| Journal | Non-Coding RNA. 2022. |
| Authors | Emel Rothzerg, Jiake Xu, and David Wood |
| Title | Zona Pellucida sperm-binding protein 3 receptor distribution during Gopc−/− globozoospermic spermatogenesis |
| Journal | Microscopy Research & Technique. 2021. |
| Authors | Maider Bizkarguenaga,Laura Gomez-Santos,Juan Francisco Madrid,Francisco José Sáez,Edurne Alonso |
| Title | The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues |
| Journal | BMC Cancer. 2019. |
| Authors | Michal Marczyk, Chunxiao Fu, Rosanna Lau, Lili Du, Alexander J. Trevarton, Bruno V. Sinn, Rebekah E. Gould, Lajos Pusztai, Christos Hatzis & W. Fraser Symmans |
| Title | miR-17-92a-1 cluster host gene (MIR17HG) evaluation and response to neoadjuvant chemoradiotherapy in rectal cancer |
| Journal | OncoTargets and Therapy. 2016. |
| Authors | Molinari C, Salvi S, Foca F, Teodorani N, Saragoni L, Puccetti M, Passardi A, Tamberi S, Avanzolini A, Lucci E, Calistri D |
| Title | Involucrin Expression and Association with Ki-67 in Paraffin Embedded Tissue of Canine Skin Tumors: A Retrospective Study |
| Journal | The Thai Journal of Veterinary Medicine. 2016. |
| Authors | Assawawongkasem, N., Suriyaphol, G., Srisuwatanasagul, S., Theerawattanasirikul, S., & Sailasuta, A |
| Title | Correlative Analysis of miRNA Expression and Oncotype Dx Recurrence Score in Estrogen Receptor Positive Breast Carcinomas |
| Journal | PLOS ONE. 2015. |
| Authors | Rajyasree Emmadi , Emanuele Canestrari, Zarema H. Arbieva, Wenbo Mu, Yang Dai, Jonna Frasor, Elizabeth Wiley |
| Title | Ocoxin® oral solution slows down tumor growth in an experimental model of colorectal cancer metastasis to the liver in Balb/c mice. |
| Journal | Oncology Reports. 2015. |
| Authors | Márquez J, Mena J, Hernandez-Unzueta I, Benedicto A, Sanz E, Arteta B, Olaso E |
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | Telomeric repeat-containing RNA (TERRA) constitutes a nucleoprotein component of extracellular inflammatory exosomes |
| Journal | PNAS. 2015. |
| Authors | Zhuo Wang, Zhong Deng, Nadia Dahmane, Kevin Tsai, Pu Wang, Dewight R. Williams, Andrew V. Kossenkov, Louise C. Showe, Rugang Zhang, Qihong Huang, José R. Conejo-Garcia, and Paul M. Lieberman |
| Title | A phase II and pharmacodynamic study of sunitinib in relapsed/refractory oesophageal and gastro-oesophageal cancers |
| Journal | British Journal of Cancer. 2015. |
| Authors | C Wu, S Mikhail, L Wei, C Timmers, S Tahiri, A Neal, J Walker, S El-Dika, M Blazer, J Rock, D J Clark, X Yang, J L Chen, J Liu, M V Knopp and T Bekaii-Saab |
| Title | MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies |
| Journal | Journal of Thoracic Oncology. 2015. |
| Authors | Patnaik, Santosh, Mallick, Reema, Kannisto, Eric MS, Sharma, Rohit, Bshara, Wiam, Yendamuri, Sai M, Dhillon, Samjot Singh |
| Title | LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis |
| Journal | The Journal of Clinical Investigation. 2014. |
| Authors | Shi-Dong Ma, Xuequn Xu, Julie Plowshay, Erik A. Ranheim, William J. Burlingham, Jeffrey L. Jensen, Fotis Asimakopoulos, Weihua Tang, Margaret L. Gulley, Ethel Cesarman, Jenny E. Gumperz and Shannon C. Kenney |
| Title | Robust global microRNA expression profiling using next-generation sequencing technologies. |
| Journal | Laboratory Investigation. 2014. |
| Authors | Tam S, Borja R, Tsao M, and McPherson J. |
| Title | Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. |
| Journal | BMC Research Notes. 2012. |
| Authors | Patnaik SK, Kannisto E, Yendamuri S. |
| Title | Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. |
| Journal | PLoS One. 2011. |
| Authors | Patnaik S, Kannisto E, Mallick R, Yendamuri S. |
High Throughput (25400)
| Title | Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. |
| Journal | BMC Research Notes. 2012. |
| Authors | Patnaik SK, Kannisto E, Yendamuri S. |
| Title | Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. |
| Journal | PLoS One. 2011. |
| Authors | Patnaik S, Kannisto E, Mallick R, Yendamuri S. |
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