Rapid high throughput extraction and purification of total RNA and microRNA from leukocytes

  • Fractionate leukocytes from whole blood in minutes
  • Isolate total RNA, including microRNA, without phenol
  • Rapid and convenient 96-well plate format for high throughput applications
  • Recovered RNA is suitable for downstream applications
  • No phenol:chloroform extractions
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

Related Services


NGS Service RNA Isolation Services

ItemsCat. #Size
Leukocyte RNA Purification Kit 96-Well Kit 37800 2 x 96-well plates

Leukocyte RNA Purification 96-Well Kit

Norgen’s Leukocyte RNA Purification Kit provides a rapid method for the high throughput isolation and purification of total leukocyte RNA from mammalian blood samples. Selective isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The kit is able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation.  Norgen's kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Maximum Binding Capacity Per Well 
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including
small RNA < 200 nt
Maximum Blood Input*
1 mL or 1.5 x 106 Leukocytes
Standard Blood Input
150 μL
Minimum Blood Input
10 μL
Average Yield:
500 μL human blood
1.5 μg
Time to Complete 10 Purification
40 minutes

*Additional RBC Lysis Solution is required for input Volumes >150 µL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.