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Leukocyte RNA Purification Kit (Cat. 21200)

 

Purchasing Information

  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • Isolate total RNA, including microRNA, without phenol
  • Rapid and convenient spin-column format
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more 
  • To process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL see the Leukocyte RNA Purification Plus Kit (Cat. 21250)
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Related Services


NGS

RNA Isolation

There is a new higher input format for this product: Leukocyte RNA Purification Plus Kit (Cat. 21250)

For the rapid extraction and purification of total RNA from leukocytes

Leukocyte RNA Purification Kit (Cat. 21200) 50 preps

  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • Isolate total RNA, including microRNA, without phenol
  • Rapid and convenient spin-column format
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more 
  • To process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL see the Leukocyte RNA Purification Plus Kit (Cat. 21250)
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Related Services


NGS

RNA Isolation


Leukocyte RNA Purification Kit

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. The kit is supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation.  Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The kit is able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA).   The purified RNA is of the highest quality and can be used in a number of downstream applications.

Kit Specifications
Maximum Column Binding Capacity 
Up to 50 μg RNA
Maximum Loading Volume 
650 μL
Size of RNA Purified
All sizes, including small RNA <200 nt
Minimum Blood Input 
10 μL
Maximum Blood Input 
2 mL of 3 x 106 leukocytes
Time to Complete 10 Purifications
40 minutes
Input Type
Blood from Human, Hamster, Mouse, Rabbit, and Rat
Average Yield
Human Blood (500 μL)
Hamster Blood (100 μL)
 
1.5 μg
2.5 μg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.


Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

Leukocyte RNA Purification Kit (Cat. 21200) 50 preps

  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • Isolate total RNA, including microRNA, without phenol
  • Rapid and convenient spin-column format
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more 
  • To process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL see the Leukocyte RNA Purification Plus Kit (Cat. 21250)
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Related Services


NGS

RNA Isolation


Leukocyte RNA Purification Kit

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. The kit is supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation.  Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The kit is able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA).   The purified RNA is of the highest quality and can be used in a number of downstream applications.

Kit Specifications
Maximum Column Binding Capacity 
Up to 50 μg RNA
Maximum Loading Volume 
650 μL
Size of RNA Purified
All sizes, including small RNA <200 nt
Minimum Blood Input 
10 μL
Maximum Blood Input 
2 mL of 3 x 106 leukocytes
Time to Complete 10 Purifications
40 minutes
Input Type
Blood from Human, Hamster, Mouse, Rabbit, and Rat
Average Yield
Human Blood (500 μL)
Hamster Blood (100 μL)
 
1.5 μg
2.5 μg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.

Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

 

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