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![Leukocyte RNA Purification Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/21200-Reflection-web.jpg?itok=z9q_9UKv" "Leukocyte RNA Purification Kit")
For the rapid extraction and purification of total RNA from leukocytes
| Items | Cat. # | Size | |
|---|---|---|---|
| Leukocyte RNA Purification Kit | 21200 | 50 preps |
Leukocyte RNA Purification Kit
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. The kit is supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The kit is able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
Up to 50 μg RNA
|
| Maximum Loading Volume |
650 μL
|
| Size of RNA Purified |
All sizes, including small RNA <200 nt
|
| Minimum Blood Input |
10 μL
|
| Maximum Blood Input |
2 mL of 3 x 106 leukocytes
|
| Time to Complete 10 Purifications |
40 minutes
|
| Input Type |
Blood from Human, Hamster, Mouse, Rabbit, and Rat
|
| Average Yield Human Blood (500 μL) Hamster Blood (100 μL) |
1.5 μg 2.5 μg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
| Journal | Journal of Immunology. 2015. |
| Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
| Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
| Journal | Molecules. 2014. |
| Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
| Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
| Journal | Med Oncol. 2014. |
| Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
| Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
| Journal | BMC Pediatrics. 2014. |
| Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
| Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
| Journal | Journal of Immunology. 2013. |
| Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
| Title | Biodosimetry on small blood volume using gene expression assay. |
| Journal | Health Physics, 98(2):179-85. 2010. |
| Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
| Items | Cat. # | Size | |
|---|---|---|---|
| Leukocyte RNA Purification Kit | 21200 | 50 preps |
Leukocyte RNA Purification Kit
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. The kit is supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The kit is able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
Up to 50 μg RNA
|
| Maximum Loading Volume |
650 μL
|
| Size of RNA Purified |
All sizes, including small RNA <200 nt
|
| Minimum Blood Input |
10 μL
|
| Maximum Blood Input |
2 mL of 3 x 106 leukocytes
|
| Time to Complete 10 Purifications |
40 minutes
|
| Input Type |
Blood from Human, Hamster, Mouse, Rabbit, and Rat
|
| Average Yield Human Blood (500 μL) Hamster Blood (100 μL) |
1.5 μg 2.5 μg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
| Journal | Journal of Immunology. 2015. |
| Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
| Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
| Journal | Molecules. 2014. |
| Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
| Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
| Journal | Med Oncol. 2014. |
| Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
| Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
| Journal | BMC Pediatrics. 2014. |
| Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
| Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
| Journal | Journal of Immunology. 2013. |
| Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
| Title | Biodosimetry on small blood volume using gene expression assay. |
| Journal | Health Physics, 98(2):179-85. 2010. |
| Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
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