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For the rapid extraction and purification of total RNA from leukocytes
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Maximum Column Binding Capacity |
Up to 50 μg RNA
|
Maximum Loading Volume |
650 μL
|
Size of RNA Purified |
All sizes, including small RNA <200 nt
|
Minimum Blood Input |
10 μL
|
Maximum Blood Input |
2 mL or 3 x 106 leukocytes
|
Time to Complete 10 Purifications |
40 minutes
|
Input Type |
Blood from Human, Hamster, Mouse, Rabbit, and Rat
|
Average Yield Human Blood (500 μL) Hamster Blood (100 μL) |
1.5 μg 2.5 μg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The RBC Lysis Buffer should be stored at 4°C upon arrival.
Kit Specifications
|
|
Maximum Column Binding Capacity |
10 µg
|
Maximum Column Loading Volume |
650 μL
|
Size of RNA Purified |
All sizes, including small RNA (<200 nt)
|
Maximum Blood Input Volume |
3 mL
|
Time to Complete 10 Purifications |
40 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Kit Specifications
|
|
Maximum Binding Capacity Per Well
|
50 μg
|
Maximum Loading Volume Per Well |
500 μL
|
Size of RNA Purified |
All sizes, including
small RNA < 200 nt |
Maximum Blood Input* |
1 mL or 1.5 x 106 Leukocytes
|
Standard Blood Input |
150 μL
|
Minimum Blood Input |
10 μL
|
Average Yield: 500 μL human blood |
1.5 μg |
Time to Complete 10 Purification |
40 minutes
|
*Additional RBC Lysis Solution is required for input Volumes >150 µL
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Supporting Data
Spin Column (21200)
Title | Effects of Omega-3 Supplementation on Components of the Endocannabinoid System and Metabolic and Inflammatory Responses in Adipose and Liver of Peripartum Dairy Cows |
Journal | Research Square. 2022. |
Authors | Gitit Kra, Jayasimha Rayalu Daddam, Uzi Moallem, Hadar Kamer, Radka Kočvarová, Alina Nemirovski, G. Andres Contreras, Joseph Tam and Maya Zachut |
Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
Journal | Molecular Cancer. 2015. |
Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
Journal | Journal of Immunology. 2015. |
Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
Journal | Molecules. 2014. |
Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
Journal | Med Oncol. 2014. |
Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
Journal | BMC Pediatrics. 2014. |
Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
Journal | Journal of Immunology. 2013. |
Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
Title | Biodosimetry on small blood volume using gene expression assay. |
Journal | Health Physics, 98(2):179-85. 2010. |
Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
Plus (21250)
Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
Journal | Molecular Cancer. 2015. |
Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
Journal | Journal of Immunology. 2015. |
Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
Journal | Molecules. 2014. |
Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
Journal | Med Oncol. 2014. |
Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
Journal | BMC Pediatrics. 2014. |
Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
Journal | Journal of Immunology. 2013. |
Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
Title | Biodosimetry on small blood volume using gene expression assay. |
Journal | Health Physics, 98(2):179-85. 2010. |
Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
Leukocyte RNA Purification Kits
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen's kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Maximum Column Binding Capacity |
Up to 50 μg RNA
|
Maximum Loading Volume |
650 μL
|
Size of RNA Purified |
All sizes, including small RNA <200 nt
|
Minimum Blood Input |
10 μL
|
Maximum Blood Input |
2 mL or 3 x 106 leukocytes
|
Time to Complete 10 Purifications |
40 minutes
|
Input Type |
Blood from Human, Hamster, Mouse, Rabbit, and Rat
|
Average Yield Human Blood (500 μL) Hamster Blood (100 μL) |
1.5 μg 2.5 μg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The RBC Lysis Buffer should be stored at 4°C upon arrival.
Kit Specifications
|
|
Maximum Column Binding Capacity |
10 µg
|
Maximum Column Loading Volume |
650 μL
|
Size of RNA Purified |
All sizes, including small RNA (<200 nt)
|
Maximum Blood Input Volume |
3 mL
|
Time to Complete 10 Purifications |
40 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Kit Specifications
|
|
Maximum Binding Capacity Per Well
|
50 μg
|
Maximum Loading Volume Per Well |
500 μL
|
Size of RNA Purified |
All sizes, including
small RNA < 200 nt |
Maximum Blood Input* |
1 mL or 1.5 x 106 Leukocytes
|
Standard Blood Input |
150 μL
|
Minimum Blood Input |
10 μL
|
Average Yield: 500 μL human blood |
1.5 μg |
Time to Complete 10 Purification |
40 minutes
|
*Additional RBC Lysis Solution is required for input Volumes >150 µL
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Spin Column (21200)
Title | Effects of Omega-3 Supplementation on Components of the Endocannabinoid System and Metabolic and Inflammatory Responses in Adipose and Liver of Peripartum Dairy Cows |
Journal | Research Square. 2022. |
Authors | Gitit Kra, Jayasimha Rayalu Daddam, Uzi Moallem, Hadar Kamer, Radka Kočvarová, Alina Nemirovski, G. Andres Contreras, Joseph Tam and Maya Zachut |
Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
Journal | Molecular Cancer. 2015. |
Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
Journal | Journal of Immunology. 2015. |
Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
Journal | Molecules. 2014. |
Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
Journal | Med Oncol. 2014. |
Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
Journal | BMC Pediatrics. 2014. |
Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
Journal | Journal of Immunology. 2013. |
Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
Title | Biodosimetry on small blood volume using gene expression assay. |
Journal | Health Physics, 98(2):179-85. 2010. |
Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
Plus (21250)
Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
Journal | Molecular Cancer. 2015. |
Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
Journal | Journal of Immunology. 2015. |
Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
Journal | Molecules. 2014. |
Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
Journal | Med Oncol. 2014. |
Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
Journal | BMC Pediatrics. 2014. |
Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
Journal | Journal of Immunology. 2013. |
Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
Title | Biodosimetry on small blood volume using gene expression assay. |
Journal | Health Physics, 98(2):179-85. 2010. |
Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |