Leukocyte RNA Purification Kits

Cat. 21200, 21250, 37800
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Leukocyte RNA Purification Kits

Cat. 21200, 21250, 37800

For the rapid extraction and purification of total RNA from leukocytes

  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • Isolate total RNA, including microRNA, without phenol
  • Rapid and convenient spin-column format and 96-well plate for high throughput applications
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Leukocyte RNA Purification Kit
(Cat. 21200)
50 preps
Leukocyte RNA Purification Plus Kit
(Cat. 21250)
50 preps
Leukocyte RNA Purification Kit 96-Well Kit
(Cat. 37800)
2 x 96-well plates

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • Isolate total RNA, including microRNA, without phenol
  • Rapid and convenient spin-column format and 96-well plate for high throughput applications
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Related Services

Leukocyte RNA Purification Kits

These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Leukocyte RNA Purification Kit

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes

Leukocyte RNA Purification Plus Kit

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.

Leukocyte RNA Purification 96-Well Kit

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen's kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.

Kit Specifications

You have selected: Cat. 21200
Kit Specifications
Maximum Column Binding Capacity 
Up to 50 μg RNA
Maximum Loading Volume 
650 μL
Size of RNA Purified
All sizes, including small RNA <200 nt
Minimum Blood Input 
10 μL
Maximum Blood Input 
2 mL of 3 x 106 leukocytes
Time to Complete 10 Purifications
40 minutes
Input Type
Blood from Human, Hamster, Mouse, Rabbit, and Rat
Average Yield
Human Blood (500 μL)
Hamster Blood (100 μL)
 
1.5 μg
2.5 μg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The RBC Lysis Buffer should be stored at 4°C upon arrival.

You have selected: Cat. 21250
Kit Specifications
Maximum Column Binding Capacity
10 µg
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small  RNA (<200 nt)
Maximum Blood Input Volume
3 mL
Time to Complete 10 Purifications
40 minutes

Storage Conditions and Product Stability 

All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. 37800
Kit Specifications
Maximum Binding Capacity Per Well 
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including
small RNA < 200 nt
Maximum Blood Input*
1 mL or 1.5 x 106 Leukocytes
Standard Blood Input
150 μL
Minimum Blood Input
10 μL
Average Yield:
500 μL human blood

1.5 μg
Time to Complete 10 Purification
40 minutes

*Additional RBC Lysis Solution is required for input Volumes >150 µL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.

Supporting Data

Click for expanded view

Component Cat. 21200 (50 preps) Cat. 21250 (50 preps) Cat. 37800 (2 x 96 preps)
RBC Lysis Buffer 2 x 100 mL 2 x 1 L 2 x 90 mL
Buffer RL 30 mL 30 mL -
Binding Solution - - 2 x 40 mL
Wash Solution A 38 mL 38 mL -
Wash Solution - - 2 x 20 mL
Elution Solution A 6 mL 6 mL -
Elution Solution - - 2 x 20 mL
Enzyme Incubation Buffer - 6 mL 1
DNase I - 1 Vial -
Mini Spin Columns 50 - -
Lysate Homogenization Column - 50 -
Single Cell RNA Column - 50 -
RBC Lysis 96-Well Plate - - 2
96-Well Filter Plate - - 2
Adhesive Tape - - 4
Collection Tubes 50 100 -
96-Well Collection Plate - - 2
Elution Tubes (1.7 mL) 50 50 -
96-Well Elution Plate - - 2
Product Insert 1 1 1

Regular (21200)

Title Effects of Omega-3 Supplementation on Components of the Endocannabinoid System and Metabolic and Inflammatory Responses in Adipose and Liver of Peripartum Dairy Cows
Journal Research Square. 2022.
Authors Gitit Kra, Jayasimha Rayalu Daddam, Uzi Moallem, Hadar Kamer, Radka Kočvarová, Alina Nemirovski, G. Andres Contreras, Joseph Tam and Maya Zachut
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

Plus (21250)

Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

Leukocyte RNA Purification Kits

These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Leukocyte RNA Purification Kit

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes

Leukocyte RNA Purification Plus Kit

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.

Leukocyte RNA Purification 96-Well Kit

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen's kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 21200
Kit Specifications
Maximum Column Binding Capacity 
Up to 50 μg RNA
Maximum Loading Volume 
650 μL
Size of RNA Purified
All sizes, including small RNA <200 nt
Minimum Blood Input 
10 μL
Maximum Blood Input 
2 mL of 3 x 106 leukocytes
Time to Complete 10 Purifications
40 minutes
Input Type
Blood from Human, Hamster, Mouse, Rabbit, and Rat
Average Yield
Human Blood (500 μL)
Hamster Blood (100 μL)
 
1.5 μg
2.5 μg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The RBC Lysis Buffer should be stored at 4°C upon arrival.

You have selected: Cat. 21250
Kit Specifications
Maximum Column Binding Capacity
10 µg
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small  RNA (<200 nt)
Maximum Blood Input Volume
3 mL
Time to Complete 10 Purifications
40 minutes

Storage Conditions and Product Stability 

All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. 37800
Kit Specifications
Maximum Binding Capacity Per Well 
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including
small RNA < 200 nt
Maximum Blood Input*
1 mL or 1.5 x 106 Leukocytes
Standard Blood Input
150 μL
Minimum Blood Input
10 μL
Average Yield:
500 μL human blood

1.5 μg
Time to Complete 10 Purification
40 minutes

*Additional RBC Lysis Solution is required for input Volumes >150 µL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.

Component Cat. 21200 (50 preps) Cat. 21250 (50 preps) Cat. 37800 (2 x 96 preps)
RBC Lysis Buffer 2 x 100 mL 2 x 1 L 2 x 90 mL
Buffer RL 30 mL 30 mL -
Binding Solution - - 2 x 40 mL
Wash Solution A 38 mL 38 mL -
Wash Solution - - 2 x 20 mL
Elution Solution A 6 mL 6 mL -
Elution Solution - - 2 x 20 mL
Enzyme Incubation Buffer - 6 mL 1
DNase I - 1 Vial -
Mini Spin Columns 50 - -
Lysate Homogenization Column - 50 -
Single Cell RNA Column - 50 -
RBC Lysis 96-Well Plate - - 2
96-Well Filter Plate - - 2
Adhesive Tape - - 4
Collection Tubes 50 100 -
96-Well Collection Plate - - 2
Elution Tubes (1.7 mL) 50 50 -
96-Well Elution Plate - - 2
Product Insert 1 1 1

Regular (21200)

Title Effects of Omega-3 Supplementation on Components of the Endocannabinoid System and Metabolic and Inflammatory Responses in Adipose and Liver of Peripartum Dairy Cows
Journal Research Square. 2022.
Authors Gitit Kra, Jayasimha Rayalu Daddam, Uzi Moallem, Hadar Kamer, Radka Kočvarová, Alina Nemirovski, G. Andres Contreras, Joseph Tam and Maya Zachut
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

Plus (21250)

Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

 


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