- Products
![#](https://norgenbiotek.com/sites/default/files/styles/original/public/21250-temp.jpg?itok=HtAzahO7" "Leukocyte RNA Purification Kit")
For the rapid extraction and purification of total RNA from leukocytes
| Items | Cat. # | Size | |
|---|---|---|---|
| Leukocyte RNA Purification Plus Kit | 21250 | 50 preps |
Leukocyte RNA Purification Plus Kit
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. RNA isolated from blood can be used in various expression studies including those focusing on diseases. However, a major problem with blood RNA isolation is that a large portion of the RNA present is globin mRNA, which is found primarily in red blood cells. In fact, up to 70% of the mass of mRNA in whole blood total RNA is globin transcripts. Therefore, it is desirable to be able to remove the red blood cells from the sample and isolate only the RNA associated with the leukocytes, which will result in improved expression profiling and other applications by removing the masking effects of this abundant globin mRNA. Norgen’s Leukocyte RNA Purification Plus Kit can be used to isolate and purify total leukocyte RNA, including all small RNAs, from mammalian blood samples.
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
10 µg
|
| Maximum Column Loading Volume |
650 μL
|
| Size of RNA Purified |
All sizes, including small RNA (<200 nt)
|
| Maximum Blood Input Volume |
3 mL
|
| Time to Complete 10 Purifications |
40 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
| Journal | Journal of Immunology. 2015. |
| Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
| Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
| Journal | Molecules. 2014. |
| Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
| Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
| Journal | Med Oncol. 2014. |
| Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
| Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
| Journal | BMC Pediatrics. 2014. |
| Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
| Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
| Journal | Journal of Immunology. 2013. |
| Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
| Title | Biodosimetry on small blood volume using gene expression assay. |
| Journal | Health Physics, 98(2):179-85. 2010. |
| Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
| Items | Cat. # | Size | |
|---|---|---|---|
| Leukocyte RNA Purification Plus Kit | 21250 | 50 preps |
Leukocyte RNA Purification Plus Kit
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. RNA isolated from blood can be used in various expression studies including those focusing on diseases. However, a major problem with blood RNA isolation is that a large portion of the RNA present is globin mRNA, which is found primarily in red blood cells. In fact, up to 70% of the mass of mRNA in whole blood total RNA is globin transcripts. Therefore, it is desirable to be able to remove the red blood cells from the sample and isolate only the RNA associated with the leukocytes, which will result in improved expression profiling and other applications by removing the masking effects of this abundant globin mRNA. Norgen’s Leukocyte RNA Purification Plus Kit can be used to isolate and purify total leukocyte RNA, including all small RNAs, from mammalian blood samples.
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
10 µg
|
| Maximum Column Loading Volume |
650 μL
|
| Size of RNA Purified |
All sizes, including small RNA (<200 nt)
|
| Maximum Blood Input Volume |
3 mL
|
| Time to Complete 10 Purifications |
40 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth |
| Journal | Journal of Immunology. 2015. |
| Authors | Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S |
| Title | Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation. |
| Journal | Molecules. 2014. |
| Authors | Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj. |
| Title | Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients. |
| Journal | Med Oncol. 2014. |
| Authors | Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A. |
| Title | Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. |
| Journal | BMC Pediatrics. 2014. |
| Authors | Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS. |
| Title | Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation. |
| Journal | Journal of Immunology. 2013. |
| Authors | Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI. |
| Title | Biodosimetry on small blood volume using gene expression assay. |
| Journal | Health Physics, 98(2):179-85. 2010. |
| Authors | Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F. |
Copyright © 2020 Norgen Biotek Corp.