Contract Manufacturing

Have a product in mind? Ask the experts! Request a Quote

Leukocyte RNA Purification Plus Kit (Cat. 21250)

 

Purchasing Information

  • Process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL
  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • No phenol or chloroform extractions
  • Isolate total leukocyte RNA, including all small RNA species
  • Rapid and convenient spin-column format
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Related Services


NGS

RNA Isolation

For the rapid extraction and purification of total RNA from leukocytes

Leukocyte RNA Purification Plus Kit (Cat. 21250) 50 preps

  • Process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL
  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • No phenol or chloroform extractions
  • Isolate total leukocyte RNA, including all small RNA species
  • Rapid and convenient spin-column format
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Related Services


NGS

RNA Isolation


Leukocyte RNA Purification Plus Kit

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. RNA isolated from blood can be used in various expression studies including those focusing on diseases. However, a major problem with blood RNA isolation is that a large portion of the RNA present is globin mRNA, which is found primarily in red blood cells. In fact, up to 70% of the mass of mRNA in whole blood total RNA is globin transcripts. Therefore, it is desirable to be able to remove the red blood cells from the sample and isolate only the RNA associated with the leukocytes, which will result in improved expression profiling and other applications by removing the masking effects of this abundant globin mRNA. Norgen’s Leukocyte RNA Purification Plus Kit can be used to isolate and purify total leukocyte RNA, including all small RNAs, from mammalian blood samples.

Kit Specifications
Maximum Column Binding Capacity
10 µg
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small  RNA (<200 nt)
Maximum Blood Input Volume
3 mL
Time to Complete 10 Purifications
40 minutes

Storage Conditions and Product Stability 

All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.


Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

Leukocyte RNA Purification Plus Kit (Cat. 21250) 50 preps

  • Process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 µL
  • Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
  • No phenol or chloroform extractions
  • Isolate total leukocyte RNA, including all small RNA species
  • Rapid and convenient spin-column format
  • Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Related Services


NGS

RNA Isolation


Leukocyte RNA Purification Plus Kit

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. RNA isolated from blood can be used in various expression studies including those focusing on diseases. However, a major problem with blood RNA isolation is that a large portion of the RNA present is globin mRNA, which is found primarily in red blood cells. In fact, up to 70% of the mass of mRNA in whole blood total RNA is globin transcripts. Therefore, it is desirable to be able to remove the red blood cells from the sample and isolate only the RNA associated with the leukocytes, which will result in improved expression profiling and other applications by removing the masking effects of this abundant globin mRNA. Norgen’s Leukocyte RNA Purification Plus Kit can be used to isolate and purify total leukocyte RNA, including all small RNAs, from mammalian blood samples.

Kit Specifications
Maximum Column Binding Capacity
10 µg
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small  RNA (<200 nt)
Maximum Blood Input Volume
3 mL
Time to Complete 10 Purifications
40 minutes

Storage Conditions and Product Stability 

All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title Novel Noncompetitive IL-1 Receptor-Biased Ligand Prevents Infection- and Inflammation-Induced Preterm Birth
Journal Journal of Immunology. 2015.
Authors Nadeau-Vallée M, Quiniou C, Palacios J, Hou X, Erfani A, Madaan A, Sanchez M, Leimert K, Boudreault A, Duhamel F, Rivera JC, Zhu T, Noueihed B, Robertson SA, Ni X, Olson DM, Lubell W, Girard S, Chemtob S
Title Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
Journal Molecules. 2014.
Authors Balcerczyk, Gajewska, Macierzyńska-Piotrowska, Pawelczyk, Bartosz, Szemraj.
Title Increased TNF α, IL-6 and ErbB2 mRNA expression in peripheral blood leukocytes from breast cancer patients.
Journal Med Oncol. 2014.
Authors Alokail MS, Al-Daghri NM, Mohammed AK, Vanhoutte P, Alenad A.
Title Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Journal BMC Pediatrics. 2014.
Authors Al-Daghri NM, Abd-Alrahman S, Draz H, Alkharfy K, Mohammed AK, Clerici MS, Alokail MS.
Title Targeted Inhibition of Serotonin Type 7 (5-HT7) Receptor Function Modulates Immune Responses and Reduces the Severity of Intestinal Inflammation.
Journal Journal of Immunology. 2013.
Authors Kim JJ, Bridle BW, Ghia JE, Wang H, Syed SN, Manocha MM, Rengasamy P, Shajib MS, Wan Y, Hedlund PB, Khan WI.
Title Biodosimetry on small blood volume using gene expression assay.
Journal Health Physics, 98(2):179-85. 2010.
Authors Brengues M, Paap B, Bittner M, Amundson S, Seligmann B, Korn R, Lenigk R, Zenhausern F.

 

Related Products