Plasma/Serum cfc-RNA Advanced Purification Kit

Overview

Versatile plasma/serum input ranging from 1 mL to 6 mL
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL
Purify superior-quality and superior quantity RNA in 45 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit provides a fast, reliable and simple procedure for isolating the highest quality and quantity of all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal DNA contamination. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-RNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-RNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Details

Supporting Data

Figure 1. A representative Bioanalyzer RNA Pico Chip trace showing the quality/quantity of the RNA purified from 4 mL K2 EDTA plasma using A) Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit (Automated Isolation), B) Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit (Manual Isolation), C) Competitor Q's cfc-DNA/RNA Kit and D) Competitor Q's Circulating Nucleic Acid Kit. All purification methods showed the correct cf-RNA size on the bioanalyzer trace however the amount of the cf-RNA recovered using Norgen’s technology (as can be noticed by the height of the cf-RNA peak) was significantly higher than that for the cf-RNA purified using the silica-based technology.

Figure 2. rt-qPCR amplification of miR-21 and miR-16 from RNA purified from K2EDTA plasma using different cfc-RNA purification methods. Norgen’s SiC technology, manual and automated procedure, was superior as compared to the amplification of the same miRNA targets amplified from RNA purified from K2EDTA plasma using the Silica-based technology represented by Qiagen’s purification kits.

Kit Specifications
Minimum Plasma/Serum Input	1 mL
Maximum Plasma/Serum Input	6 mL
Size of cfc-RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	25-50 µL
Time to Complete 10 Purifications	45 minutes
Average Yields*	Variable depending on specimen

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Kit Components	Cat. 68200 (50 preps)
Binding Buffer A	20 mL
Proteinase K	6.5 mL
Slurry E	12.5 mL
Lysis Buffer A	130 mL
Wash Solution A	38 mL
Elution Buffer C	2 x 8 mL
Elution Solution A	20 mL
Micro Spin Columns	50
Mini Filter Spin Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	100
Product Insert	1

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Plasma/Serum cfc-RNA Advanced Purification Kit