For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma and serum input volumes (0.25 mL to 5 mL)
  • Concentrate circulating and exosomal RNA into small elution volumes
  • Isolate inhibitor-free circulating RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias

ItemsCat. #Size
Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) 42800 50 Preps

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL. This kit is able to isolate all sizes of circulating and exosmal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.

Preparation time for a single sample is less than 40 minutes. The purified plasma/serum free-circulating and exosmal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

Kit Specifications
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 40 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed.


Kit Components
Component Cat. 42800 (50 preps)
Slurry C2 12 mL
Lysis Buffer A 2 x 130 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
Journal Journal of Extracellular Vesicles. 2017.
Authors Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S.
Title Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles
Journal Thrombosis Research. 2017.
Authors Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz
Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma
Journal The Journal of Molecular Diagnostics. 2017.
Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano
Title MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia
Journal International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017.
Authors Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal OncoTargets and Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation
Journal Mediators of Inflammation. 2016.
Authors Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue
Title microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection
Journal Scientific Reports. 2016.
Authors Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM
Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR
Journal Oncology Letters. 2016.
Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA
Title Data on individual PCR efficiency values as quality control for circulating miRNAs
Journal Data in Brief. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas
Journal Cancer Prevention Research. 2015.
Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes.
Journal Modern Pathology. 2014.
Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos.
Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood.
Journal Journal of Extracellular Vesicles. 2014.
Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF.
Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels.
Journal Journal of the American Chemical Society. 2013.
Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV.