Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format)

Cat. 42800, 51000, 29500, 50900
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Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format)

Cat. 42800, 51000, 29500, 50900

For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma and serum input volumes
  • Concentrate circulating and exosomal RNA into small elution volumes
  • Isolate inhibitor-free circulating and exosomal RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Available in Spin Column or 96-well plate formats
  • Process 96-well plates using vacuum or centrifugation
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Compatible with Streck Cell-Free RNA BCT® Tubes
  • Purification is based on Norgen’s proprietary resin matrix

For research use only and NOT intended for in vitro diagnostics.

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Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format)
(Cat. 42800)
50 Preps
Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry)
(Cat. 51000)
50 preps
Plasma/Serum Circulating and Exosomal RNA Purification 96-well Kit (Slurry Format)
(Cat. 29500)
1 x 96-well plate
Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format)
(Cat. 50900)
25 Preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 42800
Kit Specifications
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete 10 Purifications 
< 40 minutes

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Slurry C2 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry C2 contains a grey resin which will not disappear with warming up.

You have selected: Cat. 51000
Kit Specifications - Spin Column
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
2 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 40 minutes

Cat.51000 Storage Conditions and Product Stability
It is recommended to warm up Slurry C1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation (crystallization) is observed. Slurry C1 contains grey resin that will not disappear by warming up.

You have selected: Cat. 29500
Kit Specifications - 96-well
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
2 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 1 hour

 

Cat.29500 Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 50900
Kit Specifications
Minimum Plasma/Serum Input
2 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt) 
Time to Complete Purification
< 40 minutes

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Mini, High Throughput (51000, 29500)

Title Lateral Flow Test (LFT) Detects Cell-Free MicroRNAs Predictive of Preterm Birth Directly from Human Plasma
Journal Advanced NanoBiomed Research. 2022.
Authors Loukia Petrou, Elmeri Latvanen, Florent Seichepine, Sung Hye Kim, Phillip R. Bennett, Lynne Sykes, David A. MacIntyre, Vasso Terzidou, Sylvain Ladame
Title A pilot study to troubleshoot quality control metrics when assessing circulating miRNA expression data reproducibility across study sites
Journal Cancer Biomarkers. 2022.
Authors Permuth, Jennifer B. et al.
Title Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts
Journal PNAS . 2022.
Authors Krishna Chaitanya Pavani, Tim Meese, Osvaldo Bogado Pascottini, XueFeng Guan, Xiaoyuan Lin, Luc Peelman, Joachim Hamacher, Filip Van Nieuwerburgh, Dieter Deforce, Annekatrien Boel, Bjorn Heindryckx, Kelly Tilleman, Ann Van Soom, Bart M. Gadella, An Hendrix and Katrien Smits
Title Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration
Journal JCI Insight. . 2022.
Authors Kyle V Marra, Edith Aguilar, Wei Guoqin, Ayumi Usui-Ouchi, Yochiro Ideguchi, Susumu Sakimoto, Martin Friedlander
Title Clinical utility of cerebrospinal fluid-derived circular RNAs in lung adenocarcinoma patients with brain metastases
Journal Journal of Translational Medicine. 2022.
Authors Zhen Wang, Ruoying Yu, Xiaoxi Chen, Hua Bao, Ran Cao, An-Na Li, Qiuxiang Ou, Hai-Yan Tu, Qing Zhou, Xue Wu, Zhi-Bo Lin & Yi-Long Wu
Title Identification of key microRNAs in exosomes derived from patients with the severe acute pancreatitis
Journal Asian Journal of Surgery. 2022.
Authors Yuanxu Qu, Yixuan Ding, Jiongdi Lu, Yuchen Jia, Chunjing Bian, Yulin Guo, Zhi Zheng, Wentong Mei, Feng Cao, Fei Li
Title Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts
Journal PNAS. 2022.
Authors Krishna Chaitanya Pavani, Tim Meese, Osvaldo Bogado Pascottini, XueFeng Guan, Xiaoyuan Lin, Luc Peelman, Joachim Hamacher, Filip Van Nieuwerburgh, Dieter Deforce, Annekatrien Boel, Bj€orn Heindryckx, Kelly Tilleman, Ann Van Soom, Bart M. Gadella, An Hendrix, and Katrien Smits
Title Deep sequencing of circulating exosomal microRNA allows non-invasive glioblastoma diagnosis
Journal NPJ Precision Oncology. 2018.
Authors Saeideh Ebrahimkhani, Fatemeh Vafaee, Susannah Hallal, Heng Wei, Maggie Yuk T. Lee, Paul E. Young, Laveniya Satgunaseelan, Heidi Beadnall, Michael H. Barnett, Brindha Shivalingam, Catherine M. Suter, Michael E. Buckland, Kimberley L. Kaufman
Title Exosomal microRNA signatures in multiple sclerosis reflect disease status
Journal Scientific Reports. 2017.
Authors Saeideh Ebrahimkhani, Fatemeh Vafaee, Paul E. Young, Suzy S. J. Hur, Simon Hawke, Emma Devenney, Heidi Beadnall, Michael H. Barnett, Catherine M. Suter & Michael E. Buckland
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal Onco Targets Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Schistosomal miRNAs isolated from Extracellular Vesicles in sera of infected patients; a new tool for diagnosis and follow-up of human schistosomiasis
Journal The Journal of Infectious Disease. 2016.
Authors Tal Meningher, Galya Lerman, Neta Regev-Rudzki, Daniel Gold, Iddo Ben-Dov, Yechezkel Sidi, Dror Avni, and Eli Schwartz
Title Plasma DNA and RNA differentially impact coagulation during abdominal sepsis – an explorative study
Journal Journal of Surgical Research. 2016.
Authors Emmanuel Schneck, Omar Samara, Christian Koch, Andreas Hecker, Winfried Padberg, Christoph Lichtenstern, Markus Alexander Weigand, Florian Uhle
Title DNA Clutch Probes for Circulating Tumor DNA Analysis
Journal Journal of the American Chemical Society. 2016.
Authors Das, J., Ivanov, I., Sargent, E. H., & Kelley, S. O
Title Variability in microRNA recovery from plasma: comparison of five commercial kits
Journal Analytical Biochemistry. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum
Journal Nature Chemistry. 2015.
Authors Jagotamoy Das, Ivaylo Ivanov, Laura Montermini, Janusz Rak, Edward H. Sargent & Shana O. Kelley
Title The JCPYV DNA load inversely correlates with the viral microrna expression in blood and cerebrospinal fluid of patients at risk of PML
Journal Journal of Clinical Virology. 2015.
Authors Arianna Rocca, Francesco Martelli, Serena Delbue, Pasquale Ferrante, Dario Bartolozzi, Alberta Azzi, Simone Giannecchini
Title Expression Profiling Of MiRNAs In Pediatric Bronchopulmonary Dysplasia
Journal American Thoracic Society International Conference Abstracts. 2015.
Authors Judie A. Howrylak, Melody Pham, Christiana Oji-Mmuo, Susan DiAngelo, Debra Spear, Kimberly Group, Roopa Siddaiah, Neal Thomas , Patricia Silveyra
Title Dose-dependent Modulation of Lipid Parameters, Cytokines and RNA by δ-tocotrienol in Hypercholesterolemic Subjects Restricted to AHA Step-1 Diet
Journal British Journal of Medicine and Medical Research. 2014.
Authors Asaf A. Qureshi, Dilshad A. Khan , Wajiha Mahjabeen and Nilofer Qureshi
Title Prognostic serum miRNA biomarkers associated with Alzheimer’s disease shows concordance with neuropsychological and neuroimaging assessment.
Journal Molecular Psychiatry. 2014.
Authors L Cheng, J D Doecke, R A Sharples, V L Villemagne, C J Fowler, A Rembach, R N Martins, C C Rowe, S L Macaulay, C L Masters, A F Hill.

Spin Column (42800)

Title Extracellular vesicle-encapsulated microRNAs as novel biomarkers of lung health
Journal AJRCCM. 2022.
Authors Christina M. Eckhardt, Simone Gambazza, Tessa R. Bloomquist, et al.
Title Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma
Journal Medicina. 2022.
Authors Cosmin Ioan Faur, Rareș Călin Roman, Ancuța Jurj, Lajos Raduly, Oana Almășan, Horațiu Rotaru, Magdalena Chirilă, Mădălina Anca Moldovan, Mihaela Hedeșiu and Cristian Dinu
Title One-Step and Real-Time Detection of microRNA-21 in Human Samples for Lung Cancer Biosensing Diagnosis
Journal Anal. Chem. 2022.
Authors Olalla Calvo-Lozano, Pablo García-Aparicio, Lajos-Zsolt Raduly, Maria Carmen Estévez, Ioana Berindan-Neagoe, Manuela Ferracin, and Laura M. Lechuga
Title Plasma cell-free RNA profiling distinguishes cancers from pre-malignant conditions in solid and hematologic malignancies
Journal npj Precision Oncology. 2022.
Authors Breeshey Roskams-Hieter, Hyun Ji Kim, Pavana Anur, Josiah T. Wagner, Rowan Callahan, Elias Spiliotopoulos, Charles Ward Kirschbaum, Fehmi Civitci, Paul T. Spellman, Reid F. Thompson, Khashayar Farsad, Willscott E. Naugler & Thuy T. M. Ngo
Title Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage
Journal Scientific Reports. 2022.
Authors Hyun Ji Kim, Matthew J. Rames, Samuel Tassi Yunga, Randall Armstrong, Mayu Morita, Anh T. P. Ngo, Owen J. T. McCarty, Fehmi Civitci, Terry K. Morgan & Thuy T. M. Ngo
Title Salivary miRNA Expression in Children With Persistent Post-concussive Symptoms
Journal Front Public Health. 2022.
Authors Katherine E. Miller, James P. MacDonald, Lindsay Sullivan, Lakshmi Prakruthi Rao Venkata, Junxin Shi, Keith Owen Yeates, Su Chen, Enas Alshaikh, H. Gerry Taylor, Amanda Hautmann, Nicole Asa, Daniel M. Cohen, Thomas L. Pommering, Elaine R. Mardis, Jingzhen Yang
Title A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
Journal Sports. 2021.
Authors Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami and Kazuhiro Takekoshi
Title RNA profiles reveal signatures of future health and disease in pregnancy
Journal Nature. 2022.
Authors Rasmussen M, Reddy M, Nolan R, Camunas-Soler J, Khodursky A, Scheller NM, Cantonwine DE, Engelbrechtsen L, Mi JD, Dutta A, Brundage T, Siddiqui F, Thao M, Gee EPS, La J, Baruch-Gravett C, Santillan MK, Deb S, Ame SM, Ali SM, Adkins M, DePristo MA, Lee M, Namsaraev E, Gybel-Brask DJ, Skibsted L, Litch JA, Santillan DA, Sazawal S, Tribe RM, Roberts JM, Jain M, Høgdall E, Holzman C, Quake SR, Elovitz MA, McElrath TF
Title Whole Blood Holding Time Prior to Plasma Processing Alters microRNA Expression Profile
Journal Frontiers Genetics . 2022.
Authors Kim SH, MacIntyre DA, Sykes L, Arianoglou M, Bennett PR, Terzidou V
Title Plasma cell-free RNA profiling distinguishes cancers from pre-malignant conditions in solid and hematologic malignancies
Journal NPJ precision oncology . 2022.
Authors Roskams-Hieter B, Kim HJ, Anur P, Wagner JT, Callahan R, Spiliotopoulos E, Kirschbaum CW, Civitci F, Spellman PT, Thompson RF, Farsad K, Naugler WE, Ngo TTM
Title Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage.
Journal Scientific Reports. 2022.
Authors Kim, H., Rames, M.J., Tassi Yunga, S. et al.
Title Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births
Journal EBioMedicine. 2020.
Authors Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V.
Title Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles
Journal Thrombosis Research. 2017.
Authors Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz
Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma
Journal The Journal of Molecular Diagnostics. 2017.
Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano
Title MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia
Journal International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017.
Authors Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal OncoTargets and Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation
Journal Mediators of Inflammation. 2016.
Authors Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue
Title microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection
Journal Scientific Reports. 2016.
Authors Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM
Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR
Journal Oncology Letters. 2016.
Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA
Title Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
Journal Journal of Extracellular Vesicles. 2015.
Authors Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S.
Title Data on individual PCR efficiency values as quality control for circulating miRNAs
Journal Data in Brief. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas
Journal Cancer Prevention Research. 2015.
Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes.
Journal Modern Pathology. 2014.
Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos.
Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood.
Journal Journal of Extracellular Vesicles. 2014.
Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF.
Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels.
Journal Journal of the American Chemical Society. 2013.
Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV.

Maxi (50900)

Title Loa loa and Onchocerca ochengi miRNAs detected in host circulation
Journal Molecular and Biochemical Parasitology. 2014.
Authors Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary.
Title Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections
Journal PLOS Neglected Tropical Diseases. 2014.
Authors Lucienne Tritten, Erica Burkman, Andrew Moorhead, Mohammed Satti, James Geary, Charles Mackenzie, Timothy Geary.
Title Loa loa and Onchocerca ochengi miRNAs detected in host circulation
Journal Molecular and Biochemical Parasitology. 2014.
Authors Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary.
Title Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections
Journal PLOS Neglected Tropical Diseases. 2014.
Authors Lucienne Tritten, Erica Burkman, Andrew Moorhead, Mohammed Satti, James Geary, Charles Mackenzie, Timothy Geary.

Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format)

These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry)

Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL.

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry)

Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL.

Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry)

Norgen's Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation.

Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry)

Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 42800
Kit Specifications
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete 10 Purifications 
< 40 minutes

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Slurry C2 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry C2 contains a grey resin which will not disappear with warming up.

You have selected: Cat. 51000
Kit Specifications - Spin Column
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
2 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 40 minutes

Cat.51000 Storage Conditions and Product Stability
It is recommended to warm up Slurry C1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation (crystallization) is observed. Slurry C1 contains grey resin that will not disappear by warming up.

You have selected: Cat. 29500
Kit Specifications - 96-well
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
2 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 1 hour

 

Cat.29500 Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 50900
Kit Specifications
Minimum Plasma/Serum Input
2 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt) 
Time to Complete Purification
< 40 minutes

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Mini, High Throughput (51000, 29500)

Title Lateral Flow Test (LFT) Detects Cell-Free MicroRNAs Predictive of Preterm Birth Directly from Human Plasma
Journal Advanced NanoBiomed Research. 2022.
Authors Loukia Petrou, Elmeri Latvanen, Florent Seichepine, Sung Hye Kim, Phillip R. Bennett, Lynne Sykes, David A. MacIntyre, Vasso Terzidou, Sylvain Ladame
Title A pilot study to troubleshoot quality control metrics when assessing circulating miRNA expression data reproducibility across study sites
Journal Cancer Biomarkers. 2022.
Authors Permuth, Jennifer B. et al.
Title Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts
Journal PNAS . 2022.
Authors Krishna Chaitanya Pavani, Tim Meese, Osvaldo Bogado Pascottini, XueFeng Guan, Xiaoyuan Lin, Luc Peelman, Joachim Hamacher, Filip Van Nieuwerburgh, Dieter Deforce, Annekatrien Boel, Bjorn Heindryckx, Kelly Tilleman, Ann Van Soom, Bart M. Gadella, An Hendrix and Katrien Smits
Title Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration
Journal JCI Insight. . 2022.
Authors Kyle V Marra, Edith Aguilar, Wei Guoqin, Ayumi Usui-Ouchi, Yochiro Ideguchi, Susumu Sakimoto, Martin Friedlander
Title Clinical utility of cerebrospinal fluid-derived circular RNAs in lung adenocarcinoma patients with brain metastases
Journal Journal of Translational Medicine. 2022.
Authors Zhen Wang, Ruoying Yu, Xiaoxi Chen, Hua Bao, Ran Cao, An-Na Li, Qiuxiang Ou, Hai-Yan Tu, Qing Zhou, Xue Wu, Zhi-Bo Lin & Yi-Long Wu
Title Identification of key microRNAs in exosomes derived from patients with the severe acute pancreatitis
Journal Asian Journal of Surgery. 2022.
Authors Yuanxu Qu, Yixuan Ding, Jiongdi Lu, Yuchen Jia, Chunjing Bian, Yulin Guo, Zhi Zheng, Wentong Mei, Feng Cao, Fei Li
Title Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts
Journal PNAS. 2022.
Authors Krishna Chaitanya Pavani, Tim Meese, Osvaldo Bogado Pascottini, XueFeng Guan, Xiaoyuan Lin, Luc Peelman, Joachim Hamacher, Filip Van Nieuwerburgh, Dieter Deforce, Annekatrien Boel, Bj€orn Heindryckx, Kelly Tilleman, Ann Van Soom, Bart M. Gadella, An Hendrix, and Katrien Smits
Title Deep sequencing of circulating exosomal microRNA allows non-invasive glioblastoma diagnosis
Journal NPJ Precision Oncology. 2018.
Authors Saeideh Ebrahimkhani, Fatemeh Vafaee, Susannah Hallal, Heng Wei, Maggie Yuk T. Lee, Paul E. Young, Laveniya Satgunaseelan, Heidi Beadnall, Michael H. Barnett, Brindha Shivalingam, Catherine M. Suter, Michael E. Buckland, Kimberley L. Kaufman
Title Exosomal microRNA signatures in multiple sclerosis reflect disease status
Journal Scientific Reports. 2017.
Authors Saeideh Ebrahimkhani, Fatemeh Vafaee, Paul E. Young, Suzy S. J. Hur, Simon Hawke, Emma Devenney, Heidi Beadnall, Michael H. Barnett, Catherine M. Suter & Michael E. Buckland
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal Onco Targets Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Schistosomal miRNAs isolated from Extracellular Vesicles in sera of infected patients; a new tool for diagnosis and follow-up of human schistosomiasis
Journal The Journal of Infectious Disease. 2016.
Authors Tal Meningher, Galya Lerman, Neta Regev-Rudzki, Daniel Gold, Iddo Ben-Dov, Yechezkel Sidi, Dror Avni, and Eli Schwartz
Title Plasma DNA and RNA differentially impact coagulation during abdominal sepsis – an explorative study
Journal Journal of Surgical Research. 2016.
Authors Emmanuel Schneck, Omar Samara, Christian Koch, Andreas Hecker, Winfried Padberg, Christoph Lichtenstern, Markus Alexander Weigand, Florian Uhle
Title DNA Clutch Probes for Circulating Tumor DNA Analysis
Journal Journal of the American Chemical Society. 2016.
Authors Das, J., Ivanov, I., Sargent, E. H., & Kelley, S. O
Title Variability in microRNA recovery from plasma: comparison of five commercial kits
Journal Analytical Biochemistry. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum
Journal Nature Chemistry. 2015.
Authors Jagotamoy Das, Ivaylo Ivanov, Laura Montermini, Janusz Rak, Edward H. Sargent & Shana O. Kelley
Title The JCPYV DNA load inversely correlates with the viral microrna expression in blood and cerebrospinal fluid of patients at risk of PML
Journal Journal of Clinical Virology. 2015.
Authors Arianna Rocca, Francesco Martelli, Serena Delbue, Pasquale Ferrante, Dario Bartolozzi, Alberta Azzi, Simone Giannecchini
Title Expression Profiling Of MiRNAs In Pediatric Bronchopulmonary Dysplasia
Journal American Thoracic Society International Conference Abstracts. 2015.
Authors Judie A. Howrylak, Melody Pham, Christiana Oji-Mmuo, Susan DiAngelo, Debra Spear, Kimberly Group, Roopa Siddaiah, Neal Thomas , Patricia Silveyra
Title Dose-dependent Modulation of Lipid Parameters, Cytokines and RNA by δ-tocotrienol in Hypercholesterolemic Subjects Restricted to AHA Step-1 Diet
Journal British Journal of Medicine and Medical Research. 2014.
Authors Asaf A. Qureshi, Dilshad A. Khan , Wajiha Mahjabeen and Nilofer Qureshi
Title Prognostic serum miRNA biomarkers associated with Alzheimer’s disease shows concordance with neuropsychological and neuroimaging assessment.
Journal Molecular Psychiatry. 2014.
Authors L Cheng, J D Doecke, R A Sharples, V L Villemagne, C J Fowler, A Rembach, R N Martins, C C Rowe, S L Macaulay, C L Masters, A F Hill.

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Title Extracellular vesicle-encapsulated microRNAs as novel biomarkers of lung health
Journal AJRCCM. 2022.
Authors Christina M. Eckhardt, Simone Gambazza, Tessa R. Bloomquist, et al.
Title Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma
Journal Medicina. 2022.
Authors Cosmin Ioan Faur, Rareș Călin Roman, Ancuța Jurj, Lajos Raduly, Oana Almășan, Horațiu Rotaru, Magdalena Chirilă, Mădălina Anca Moldovan, Mihaela Hedeșiu and Cristian Dinu
Title One-Step and Real-Time Detection of microRNA-21 in Human Samples for Lung Cancer Biosensing Diagnosis
Journal Anal. Chem. 2022.
Authors Olalla Calvo-Lozano, Pablo García-Aparicio, Lajos-Zsolt Raduly, Maria Carmen Estévez, Ioana Berindan-Neagoe, Manuela Ferracin, and Laura M. Lechuga
Title Plasma cell-free RNA profiling distinguishes cancers from pre-malignant conditions in solid and hematologic malignancies
Journal npj Precision Oncology. 2022.
Authors Breeshey Roskams-Hieter, Hyun Ji Kim, Pavana Anur, Josiah T. Wagner, Rowan Callahan, Elias Spiliotopoulos, Charles Ward Kirschbaum, Fehmi Civitci, Paul T. Spellman, Reid F. Thompson, Khashayar Farsad, Willscott E. Naugler & Thuy T. M. Ngo
Title Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage
Journal Scientific Reports. 2022.
Authors Hyun Ji Kim, Matthew J. Rames, Samuel Tassi Yunga, Randall Armstrong, Mayu Morita, Anh T. P. Ngo, Owen J. T. McCarty, Fehmi Civitci, Terry K. Morgan & Thuy T. M. Ngo
Title Salivary miRNA Expression in Children With Persistent Post-concussive Symptoms
Journal Front Public Health. 2022.
Authors Katherine E. Miller, James P. MacDonald, Lindsay Sullivan, Lakshmi Prakruthi Rao Venkata, Junxin Shi, Keith Owen Yeates, Su Chen, Enas Alshaikh, H. Gerry Taylor, Amanda Hautmann, Nicole Asa, Daniel M. Cohen, Thomas L. Pommering, Elaine R. Mardis, Jingzhen Yang
Title A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
Journal Sports. 2021.
Authors Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami and Kazuhiro Takekoshi
Title RNA profiles reveal signatures of future health and disease in pregnancy
Journal Nature. 2022.
Authors Rasmussen M, Reddy M, Nolan R, Camunas-Soler J, Khodursky A, Scheller NM, Cantonwine DE, Engelbrechtsen L, Mi JD, Dutta A, Brundage T, Siddiqui F, Thao M, Gee EPS, La J, Baruch-Gravett C, Santillan MK, Deb S, Ame SM, Ali SM, Adkins M, DePristo MA, Lee M, Namsaraev E, Gybel-Brask DJ, Skibsted L, Litch JA, Santillan DA, Sazawal S, Tribe RM, Roberts JM, Jain M, Høgdall E, Holzman C, Quake SR, Elovitz MA, McElrath TF
Title Whole Blood Holding Time Prior to Plasma Processing Alters microRNA Expression Profile
Journal Frontiers Genetics . 2022.
Authors Kim SH, MacIntyre DA, Sykes L, Arianoglou M, Bennett PR, Terzidou V
Title Plasma cell-free RNA profiling distinguishes cancers from pre-malignant conditions in solid and hematologic malignancies
Journal NPJ precision oncology . 2022.
Authors Roskams-Hieter B, Kim HJ, Anur P, Wagner JT, Callahan R, Spiliotopoulos E, Kirschbaum CW, Civitci F, Spellman PT, Thompson RF, Farsad K, Naugler WE, Ngo TTM
Title Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage.
Journal Scientific Reports. 2022.
Authors Kim, H., Rames, M.J., Tassi Yunga, S. et al.
Title Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births
Journal EBioMedicine. 2020.
Authors Kim, S. H., MacIntyre, D. A., Binkhamis, R., Cook, J., Sykes, L., Bennett, P. R., & Terzidou, V.
Title Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles
Journal Thrombosis Research. 2017.
Authors Anoop K. Enjeti, Anita Ariyarajah, Angel D'Crus, Michael Seldon, Lisa F. Lincz
Title Development and Clinical Utility of a Blood-Based Test Service for the Rapid Identification of Actionable Mutations in Non–Small Cell Lung Carcinoma
Journal The Journal of Molecular Diagnostics. 2017.
Authors Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G. Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A. Pestano
Title MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia
Journal International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2017.
Authors Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu
Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal OncoTargets and Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Pancreatic acinar cells employ miRNAs as mediators of intercellular communication to participate in the regulation of pancreatitis-associated macrophage activation
Journal Mediators of Inflammation. 2016.
Authors Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, B. Sun, Weihui Zhang, and Dongbo Xue
Title microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection
Journal Scientific Reports. 2016.
Authors Hamam R, Ali AM, Alsaleh KA, Kassem M, Alfayez M, Aldahmash A, Alajez NM
Title Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR
Journal Oncology Letters. 2016.
Authors Al-Sheikh YA, Ghneim HK, Softa KI, Al-Jobran AA, Al-Obeed O, Mohamed MA, Abdulla M, Aboul-Soud MA
Title Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
Journal Journal of Extracellular Vesicles. 2015.
Authors Laurent LC,, Abdel-Mageed AB, Adelson PD, Arango J, Balaj L,, Breakefield X,,, Carlson E, Carter BS, Majem B, Chen CC, Cocucci E, Danielson K, Courtright A, Das S, Abd Elmageed ZY, Enderle D, Ezrin A, Ferrer M, Freedman J, Galas D,, Gandhi R, Huentelman MJ, Van Keuren-Jensen K, Kalani Y, Kim Y, Krichevsky AM, Lai C,, Lal-Nag M, Laurent CD, Leonardo T, Li F, Malenica I, Mondal D, Nejad P, Patel T,, Raffai RL,, Rubio R, Skog J, Spetzler R, Sun J, Tanriverdi K, Vickers K,, Wang L, Wang Y, Wei Z, Weiner HL, Wong D, Yan IK, Yeri A, Gould S.
Title Data on individual PCR efficiency values as quality control for circulating miRNAs
Journal Data in Brief. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas
Journal Cancer Prevention Research. 2015.
Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes.
Journal Modern Pathology. 2014.
Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos.
Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood.
Journal Journal of Extracellular Vesicles. 2014.
Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF.
Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels.
Journal Journal of the American Chemical Society. 2013.
Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV.

Maxi (50900)

Title Loa loa and Onchocerca ochengi miRNAs detected in host circulation
Journal Molecular and Biochemical Parasitology. 2014.
Authors Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary.
Title Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections
Journal PLOS Neglected Tropical Diseases. 2014.
Authors Lucienne Tritten, Erica Burkman, Andrew Moorhead, Mohammed Satti, James Geary, Charles Mackenzie, Timothy Geary.
Title Loa loa and Onchocerca ochengi miRNAs detected in host circulation
Journal Molecular and Biochemical Parasitology. 2014.
Authors Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary.
Title Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections
Journal PLOS Neglected Tropical Diseases. 2014.
Authors Lucienne Tritten, Erica Burkman, Andrew Moorhead, Mohammed Satti, James Geary, Charles Mackenzie, Timothy Geary.

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