Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples Price USD$585.00 SKU 51000 Format Mini Slurry Slurry High Throughput Slurry Maxi Slurry Size 50 Preps Quantity Overview Details Documentation Citations Overview Isolate all sizes of circulating and exosomal RNA, including microRNA Versatile plasma and serum input volumes Concentrate circulating and exosomal RNA into small elution volumes Isolate inhibitor-free circulating and exosomal RNA Bind and elute all RNA irrespective of size or GC content, without bias Available in Spin Column or 96-well plate formats Process 96-well plates using vacuum or centrifugation Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Compatible with Streck Cell-Free RNA BCT® Tubes Purification is based on Norgen’s proprietary resin matrix These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum. Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry) Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL. Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry) Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL. Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry) Norgen's Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation. Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry) Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL. Details Supporting Data Previous Figure 1. Isolation and Detection of Circulating RNA from Different Plasma Volumes. Norgen's Plasma/Serum Circulating RNA Purification Mini Kit (Slurry Format) was used to isolate circulating RNA from 0.5mL, 1mL and 2mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. RNA isolated from 0.5mL plasma is represented by the Red line, RNA isolated from 1mL plasma is represented by the Green line whereas RNA isolated from 2mL plasma is represented by the Blue line. The black line corresponds to the no template control. Figure 2. Effective Isolation of Plasma Circulating RNA from Different Volumes. Norgen's Plasma/Serum Circulating RNA Purification Mini Kit (Slurry Format) was used to isolate circulating RNA from 0.5mL, 1mL and 2mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. Average Ct. values for the amplification of the 5S rRNA isolated from 0.5mL plasma is represented by the Red bar, Average Ct. values for the amplification of the 5S rRNA isolated from 1mL plasma is represented by the Green bar whereas Average Ct. values for the amplification of the 5S rRNA isolated from 2mL plasma is represented by the blue bar. Figure 3. Effective and Consistent Detection of Plasma Exosome RNA. Norgen's Plasma/Serum Circulating and Exosomal RNA Isolation Kits can effectively isolate RNA from plasma. Plasma Exosome RNA was isolated from 500 µL of human plasma prepared from blood collected on citrate, EDTA or Heparin in triplicates using Norgen's Plasma/Serum RNA Isolation Kit (blue), a ExoQuick Exosome Precipitation Reagent (green) and Qiagen’s QIAzol extraction followed by a modified Qiagen's RNeasy Mini Kit cleanup (red). Stem loop RT-qPCR using primers specific to miR-21 and miR-16 as well as the housekeeping 5S rRNA was performed. In brief, three microliters of the 100 µL isolated RNA was then subjected to a 20 µL reverse transcription using 5S rRNA, miR-21 and miR-16 stem-loop reverse primer or reveres primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21, human miR-16 and the 5S rRNA. Norgen's Plasma/Serum Exosome RNA Isolation Kit is the only product that showed consistent detection of all tested transcripts with the highest quality regardless the type of the anti-coagulant used for blood collection. Figure 4. Isolation and Detection of Circulating RNA from Different Plasma Volumes. Norgen's Plasma/Serum Circulating RNA Purification Maxi Kit (Slurry Format) was used to isolate circulating RNA from 2mL, 3mL and 5mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. RNA isolated from 2mL plasma is represented by the Red line, RNA isolated from 3mL plasma is represented by the Green line whereas RNA isolated from 5mL plasma is represented by the Blue line. The black line corresponds to the no template control. Figure 5. Effective Isolation of Plasma Circulating RNA from Different Volumes. Norgen's Plasma/Serum Circulating RNA Purification Maxi Kit (Slurry Format) was used to isolate circulating RNA from 2mL, 3mL and 5mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. Average Ct. values for the amplification of the 5S rRNA isolated from 2mL plasma is represented by the Red bar, Average Ct. values for the amplification of the 5S rRNA isolated from 3mL plasma is represented by the Green bar whereas Average Ct. values for the amplification of the 5S rRNA isolated from 5mL plasma is represented by the blue bar. Next Click for expanded view Kit Specifications - Spin Column Minimum Plasma/Serum Input 0.25 mL Maximum Plasma/Serum Input 2 mL Size of RNA Purified All sizes, including microRNA Time to Complete Purification < 40 minutes Cat.51000 Storage Conditions and Product Stability It is recommended to warm up Slurry C1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation (crystallization) is observed. Slurry C1 contains grey resin that will not disappear by warming up. Component Cat. 51000 (50 preps) Cat. 29500 (96 preps) Cat. 42800 (50 preps) Cat. 50900 (25 preps) Slurry C1 - - - 6 mL Slurry C2 12 mL - 12 mL - Slurry C3 - 20 mL - - Lysis Buffer A 2 x 130 mL 4 x 100 mL 2 x 130 mL 2 x 130 mL Wash Solution A 38 mL 2 x 38 mL 38 mL 18 mL Elution Solution A 6 mL 20 mL 6 mL 6 mL Mini Filter Spin Columns 50 - 50 25 96-Well Filter Plate - 1 - - Adhesive Tape - 1 - - Collection Tubes 50 - 50 25 96-Well Collection Plate - 1 - - Elution Tubes (1.7 mL) 50 - 50 25 96-Well Elution Plate - 1 - - Product Insert 1 1 1 1 Documentation PROTOCOLS (42800) Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry) - Protocol (50 prep) (51000) Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry) - Protocol (50 prep) (29500) Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry) - Protocol (96-well) (50900) Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry) - Protocol (25 prep) SUPPLEMENTARY PROTOCOLS Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) - Supplementary Protocol APPLICATION NOTES Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) Effect of Microrna Gc Content On RNA Purification Efficiency - Application Note Effect of microRNA GC Content on RNA Purification Efficiency SAFETY DATA SHEETS (42800) Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) - Safety Data Sheet (51000) Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) - Safety Data Sheet (29500) Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) - Protocol (96-well) - Safety Data Sheet (50900) Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) - Safety Data Sheet POSTERS Variation Between The Purification Efficiency of Microrna With Different Gc Content In Biological and Clinical Specimens RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Services NGS RNA Isolation Related Products Plasma/Serum RNA Purification Maxi Kit RNase-Free DNase I Kit Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format) For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples Price USD$585.00 SKU 51000 Format Mini Slurry Slurry High Throughput Slurry Maxi Slurry Size 50 Preps Quantity