For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma and serum input volumes (0.25 mL to 2 mL)
  • Concentrate circulating and exosomal RNA into small elution volumes
  • Isolate inhibitor-free circulating and exosomal RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Available in Spin Column or 96-well plate formats
  • Process 96-well plates using vacuum or centrifugation

ItemsCat. #Size
Plasma/Serum Circulating RNA Purification Mini Kit (Slurry) 51000 50 preps
Plasma/Serum Circulating and Exosomal RNA Purification 96-well Kit (Slurry Format) 29500 1 x 96-well plate

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format)

Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL. Norgen's Plasma/Serum Ciruclating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating ciculating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation. These kits are able to isolate all sizes of circulating RNA and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using these kits. These kits are suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.

The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Free-circulating and exosmal plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating and exosomal RNAs have the potential to provide biomarkers for other disease states. Free-circulating and exosomal RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

Kit Specifications - Spin Column
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
2 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 40 minutes
Kit Specifications - 96-well
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
2 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 1 hour

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm the slurry and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.


Kit Components
Component Cat. 51000 (50 preps)
Slurry C2 12 mL
Lysis Buffer A 2 x 130 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Component Cat. 29500 (96-well)
Slurry C3 24 mL
Lysis Buffer A 4 x 100 mL
Wash Solution A 2 x 38 mL
Elution Solution A 20 mL
96-Well Filter Plate 1
Adhesive Tape 1
96-Well Collection Plate 1
96-Well Elution Plate 1
Product Insert 1

Title Different levels of serum microRNAs in prostate cancer and benign prostatic hyperplasia: evaluation of potential diagnostic and prognostic role
Journal Onco Targets Therapy. 2016.
Authors Cochetti G, Poli G, Guelfi G, Boni A, Egidi MG, Mearini E.
Title Schistosomal miRNAs isolated from Extracellular Vesicles in sera of infected patients; a new tool for diagnosis and follow-up of human schistosomiasis
Journal The Journal of Infectious Disease. 2016.
Authors Tal Meningher, Galya Lerman, Neta Regev-Rudzki, Daniel Gold, Iddo Ben-Dov, Yechezkel Sidi, Dror Avni, and Eli Schwartz
Title Plasma DNA and RNA differentially impact coagulation during abdominal sepsis – an explorative study
Journal Journal of Surgical Research. 2016.
Authors Emmanuel Schneck, Omar Samara, Christian Koch, Andreas Hecker, Winfried Padberg, Christoph Lichtenstern, Markus Alexander Weigand, Florian Uhle
Title DNA Clutch Probes for Circulating Tumor DNA Analysis
Journal Journal of the American Chemical Society. 2016.
Authors Das, J., Ivanov, I., Sargent, E. H., & Kelley, S. O
Title Variability in microRNA recovery from plasma: comparison of five commercial kits
Journal Analytical Biochemistry. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum
Journal Nature Chemistry. 2015.
Authors Jagotamoy Das, Ivaylo Ivanov, Laura Montermini, Janusz Rak, Edward H. Sargent & Shana O. Kelley
Title Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches
Journal Methods. 2015.
Authors Natasa Zarovni, Antonietta Corrado, Paolo Guazzi, Davide Zocco, Elisa Lari, Giorgia Radano, Jekatarina Muhhina, Costanza Fondelli, Julia Gavrilova, Antonio Chiesi
Title The JCPYV DNA load inversely correlates with the viral microrna expression in blood and cerebrospinal fluid of patients at risk of PML
Journal Journal of Clinical Virology. 2015.
Authors Arianna Rocca, Francesco Martelli, Serena Delbue, Pasquale Ferrante, Dario Bartolozzi, Alberta Azzi, Simone Giannecchini
Title Expression Profiling Of MiRNAs In Pediatric Bronchopulmonary Dysplasia
Journal American Thoracic Society International Conference Abstracts. 2015.
Authors Judie A. Howrylak, Melody Pham, Christiana Oji-Mmuo, Susan DiAngelo, Debra Spear, Kimberly Group, Roopa Siddaiah, Neal Thomas , Patricia Silveyra
Title Dose-dependent Modulation of Lipid Parameters, Cytokines and RNA by δ-tocotrienol in Hypercholesterolemic Subjects Restricted to AHA Step-1 Diet
Journal British Journal of Medicine and Medical Research. 2014.
Authors Asaf A. Qureshi, Dilshad A. Khan , Wajiha Mahjabeen and Nilofer Qureshi
Title Prognostic serum miRNA biomarkers associated with Alzheimer’s disease shows concordance with neuropsychological and neuroimaging assessment.
Journal Molecular Psychiatry. 2014.
Authors L Cheng, J D Doecke, R A Sharples, V L Villemagne, C J Fowler, A Rembach, R N Martins, C C Rowe, S L Macaulay, C L Masters, A F Hill.