For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol from small input volumes
|RNA Clean-Up and Concentration Micro-Elute Kit||61000||50 preps|
RNA Clean-Up and Concentration Micro-Elute Kit
Norgen's RNA Clean-Up and Concentration Micro-Elute Kit provides a rapid method for the purification, cleanup and concentration of RNA for NGS Library preparation and excellent processing of up to 45 µg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 µL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.
|Maximum Column Binding Capacity||45 μg|
|Size of RNA Purified||All sizes, including small RNA
|Maximum Amount of starting Material||45 μg of RNA|
|Minimum Elution Volume||8 μL|
|Time to Complete 10 Purifications||20 minutes|
|Average Recovery||≥ 90%|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||(Pro)renin receptor (ATP6AP2) depletion arrests As4.1 cells in the G0/G1 phase thereby increasing formation of primary cilia|
|Journal||Journal of Cellular and Molecular Medicine. 2017|
|Authors||Wanka H, Lutze P, Staar D, Peters B, Morch A, Vogel L, Chilukoti RK, Homuth G, Sczodrok J, Bäumgen I, Peters J.|
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