For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol from small input volumes

  • Concentration of small amounts of RNA into 8 µL
  • Ideal for concentrating RNA samples prior to NGS library preparation
  • Concentrate from larger elution volumes to more manageable elution volumes
  • Ideal for concentrating RNA purified from exosomes, plasma, serum, urine, and other bodily fluids, and any RNA samples initially purified in large volumes
  • Efficient RNA cleanup from enzymatic reactions – labeling, DNase treatment and in vitro transcription
  • Cleanup of RNA isolated using different methods, including phenol/chloroform extractions
  • Fast and easy processing using rapid spin-column format in 15 minutes
  • Suitable for all sizes of RNA, including microRNA (miRNA) without bias

ItemsCat. #Size
RNA Clean-Up and Concentration Micro-Elute Kit 61000 50 preps

RNA Clean-Up and Concentration Micro-Elute Kit

Norgen's RNA Clean-Up and Concentration Micro-Elute Kit provides a rapid method for the purification, cleanup and concentration of RNA for NGS Library preparation and excellent processing of up to 45 µg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 µL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.

Kit Specifications
Maximum Column Binding Capacity 45 μg
Size of RNA Purified All sizes, including small RNA
(<200 nt)
Maximum Amount of starting Material 45 μg of RNA
Minimum Elution Volume 8 μL
Time to Complete 10 Purifications 20 minutes
Average Recovery ≥ 90%

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 61000 (50 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Column Activation Solution 30 mL
Micro-Elute RNA Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title (Pro)renin receptor (ATP6AP2) depletion arrests As4.1 cells in the G0/G1 phase thereby increasing formation of primary cilia
Journal Journal of Cellular and Molecular Medicine. 2017
Authors Wanka H, Lutze P, Staar D, Peters B, Morch A, Vogel L, Chilukoti RK, Homuth G, Sczodrok J, Bäumgen I, Peters J.