Total RNA Purification 96-Well Kit (Cat. 24300)

For rapid high-throughput purification of total RNA including microRNA

  • Extract high quality & purity total RNA including miRNA
  • Convenient high throughput format for vacuum, centrifuge or robot
  • Very consistent well-to-well RNA isolation
  • No phenol step required - isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Isolate from a wide variety of specimens

ItemsCat. #Size
Total RNA Purification 96-Well Kit 24300 2 x 96-well plates

Total RNA Purification 96-Well Kit

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Kit Specifications
Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 96 Purifications
30 minutes
RNA Yield
HeLa Cells (1 x 106 Cells)
E. coli (1 x 109 cells)
Brain (10 mg)
Liver (10 mg)
Blood (50 μL, Hamster)
15 μg
50 μg
9 μg
26 μg
1 μg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Protocols: 
24300 - Protocol (96-well)
Safety Data Sheets: 
24300 - Safety Data Sheet

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Journal Insect Biochemistry and Molecular Biology. 2016.
Authors Fishilevich E, Vélez AM, Khajuria C, Frey ML1, Hamm RL, Wang H, Schulenberg GA, Bowling AJ, Pence HE, Gandra P, Arora K, Storer NP, Narva KE, Siegfried BD
Title Association of novel nonsynonymous single nucleotide polymorphisms in ampD with cephalosporin resistance and phylogenetic variations in ampC, ampR, ompF and ompC in Enterobacter cloacae that are highly resistant to carbapenems
Journal Antimicrobial Agents and Chemotherpy. 2016.
Authors Babouee Flury B, Ellington MJ, Hopkins KL, Turton JF, Doumith M, Loy R, Staves P, Hinic V, Frei R, Woodford N
Title Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis
Journal BMC Genomics. 2015.
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Title Utility of Select Plasma MicroRNA for Disease and Cardiovascular Risk Assessment in Patients with Rheumatoid Arthritis
Journal The Journal of Rheumatology. 2015.
Authors Michelle J. Ormseth, Joseph F. Solus, Kasey C. Vickers, Annette M. Oeser, Paolo Raggi and C. Michael Stein
Title Effects of crude oil exposure and elevated temperature on the liver transcriptome of polar cod (Boreogadus saida)
Journal Aquatic Toxicology. 2015.
Authors Øivind Andersen, Marianne Frantzen, Marte Rosland, Gerrit Timmerhaus, Adrijana Skugor, Aleksei Krasnov
Title Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels.
Journal The EMBO Journal. 2014.
Authors Luca E Lorenzi, Amadou Bah1, Harry Wischnewski, Vadim Shchepachev, Charlotte Soneson, Marco Santagostino and Claus M Azzalin.
Title RNA Expression Profiling of Human iPSC-Derived Cardiomyocytes in a Cardiac Hypertrophy Model.
Journal PLOS One. 2014.
Authors Praful Aggarwal, Amy Turner, Andrea Matter, Steven J. Kattman, Alexander Stoddard, Rachel Lorier, Bradley J. Swanson, Donna K. Arnett, Ulrich Broeckel.
Title Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach.
Journal BMC Microbiology. 2013.
Authors Azizi A, Tang M, Gisonni-Lex L, Mallet L.