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![#](https://norgenbiotek.com/sites/default/files/styles/original/public/24300.jpg?itok=K3P8odvr" "Total RNA Purification 96-Well Kit")
For rapid high-throughput purification of total RNA including microRNA
| Items | Cat. # | Size | |
|---|---|---|---|
| Total RNA Purification 96-Well Kit | 24300 | 2 x 96-well plates |
Total RNA Purification 96-Well Kit
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
|
Kit Specifications
|
|
|
Maximum Binding Capacity Per Well
|
50 μg
|
|
Maximum Loading Volume Per Well
|
500 μL
|
| Size of RNA Purified |
All sizes, including small RNA < 200 nt
|
| Time to Complete 96 Purifications |
30 minutes
|
| Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster) |
15 μg
50 μg 9 μg 26 μg 1 μg |
| Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue |
100 µL
150 µL
1 x 109 cells
1 x 108 cells
40 mg 40 mg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros) |
| Journal | Insect Biochemistry and Molecular Biology. 2016. |
| Authors | Fishilevich E, Vélez AM, Khajuria C, Frey ML1, Hamm RL, Wang H, Schulenberg GA, Bowling AJ, Pence HE, Gandra P, Arora K, Storer NP, Narva KE, Siegfried BD |
| Title | Association of novel nonsynonymous single nucleotide polymorphisms in ampD with cephalosporin resistance and phylogenetic variations in ampC, ampR, ompF and ompC in Enterobacter cloacae that are highly resistant to carbapenems |
| Journal | Antimicrobial Agents and Chemotherpy. 2016. |
| Authors | Babouee Flury B, Ellington MJ, Hopkins KL, Turton JF, Doumith M, Loy R, Staves P, Hinic V, Frei R, Woodford N |
| Title | Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis |
| Journal | BMC Genomics. 2015. |
| Authors | Wenli Li, Amy Turner, Praful Aggarwal, Andrea Matter, Erin Storvick, Donna K. Arnett and Ulrich Broeckel |
| Title | Utility of Select Plasma MicroRNA for Disease and Cardiovascular Risk Assessment in Patients with Rheumatoid Arthritis |
| Journal | The Journal of Rheumatology. 2015. |
| Authors | Michelle J. Ormseth, Joseph F. Solus, Kasey C. Vickers, Annette M. Oeser, Paolo Raggi and C. Michael Stein |
| Title | Effects of crude oil exposure and elevated temperature on the liver transcriptome of polar cod (Boreogadus saida) |
| Journal | Aquatic Toxicology. 2015. |
| Authors | Øivind Andersen, Marianne Frantzen, Marte Rosland, Gerrit Timmerhaus, Adrijana Skugor, Aleksei Krasnov |
| Title | Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels. |
| Journal | The EMBO Journal. 2014. |
| Authors | Luca E Lorenzi, Amadou Bah1, Harry Wischnewski, Vadim Shchepachev, Charlotte Soneson, Marco Santagostino and Claus M Azzalin. |
| Title | RNA Expression Profiling of Human iPSC-Derived Cardiomyocytes in a Cardiac Hypertrophy Model. |
| Journal | PLOS One. 2014. |
| Authors | Praful Aggarwal, Amy Turner, Andrea Matter, Steven J. Kattman, Alexander Stoddard, Rachel Lorier, Bradley J. Swanson, Donna K. Arnett, Ulrich Broeckel. |
| Title | Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach. |
| Journal | BMC Microbiology. 2013. |
| Authors | Azizi A, Tang M, Gisonni-Lex L, Mallet L. |
| Items | Cat. # | Size | |
|---|---|---|---|
| Total RNA Purification 96-Well Kit | 24300 | 2 x 96-well plates |
Total RNA Purification 96-Well Kit
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
|
Kit Specifications
|
|
|
Maximum Binding Capacity Per Well
|
50 μg
|
|
Maximum Loading Volume Per Well
|
500 μL
|
| Size of RNA Purified |
All sizes, including small RNA < 200 nt
|
| Time to Complete 96 Purifications |
30 minutes
|
| Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster) |
15 μg
50 μg 9 μg 26 μg 1 μg |
| Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue |
100 µL
150 µL
1 x 109 cells
1 x 108 cells
40 mg 40 mg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros) |
| Journal | Insect Biochemistry and Molecular Biology. 2016. |
| Authors | Fishilevich E, Vélez AM, Khajuria C, Frey ML1, Hamm RL, Wang H, Schulenberg GA, Bowling AJ, Pence HE, Gandra P, Arora K, Storer NP, Narva KE, Siegfried BD |
| Title | Association of novel nonsynonymous single nucleotide polymorphisms in ampD with cephalosporin resistance and phylogenetic variations in ampC, ampR, ompF and ompC in Enterobacter cloacae that are highly resistant to carbapenems |
| Journal | Antimicrobial Agents and Chemotherpy. 2016. |
| Authors | Babouee Flury B, Ellington MJ, Hopkins KL, Turton JF, Doumith M, Loy R, Staves P, Hinic V, Frei R, Woodford N |
| Title | Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis |
| Journal | BMC Genomics. 2015. |
| Authors | Wenli Li, Amy Turner, Praful Aggarwal, Andrea Matter, Erin Storvick, Donna K. Arnett and Ulrich Broeckel |
| Title | Utility of Select Plasma MicroRNA for Disease and Cardiovascular Risk Assessment in Patients with Rheumatoid Arthritis |
| Journal | The Journal of Rheumatology. 2015. |
| Authors | Michelle J. Ormseth, Joseph F. Solus, Kasey C. Vickers, Annette M. Oeser, Paolo Raggi and C. Michael Stein |
| Title | Effects of crude oil exposure and elevated temperature on the liver transcriptome of polar cod (Boreogadus saida) |
| Journal | Aquatic Toxicology. 2015. |
| Authors | Øivind Andersen, Marianne Frantzen, Marte Rosland, Gerrit Timmerhaus, Adrijana Skugor, Aleksei Krasnov |
| Title | Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels. |
| Journal | The EMBO Journal. 2014. |
| Authors | Luca E Lorenzi, Amadou Bah1, Harry Wischnewski, Vadim Shchepachev, Charlotte Soneson, Marco Santagostino and Claus M Azzalin. |
| Title | RNA Expression Profiling of Human iPSC-Derived Cardiomyocytes in a Cardiac Hypertrophy Model. |
| Journal | PLOS One. 2014. |
| Authors | Praful Aggarwal, Amy Turner, Andrea Matter, Steven J. Kattman, Alexander Stoddard, Rachel Lorier, Bradley J. Swanson, Donna K. Arnett, Ulrich Broeckel. |
| Title | Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach. |
| Journal | BMC Microbiology. 2013. |
| Authors | Azizi A, Tang M, Gisonni-Lex L, Mallet L. |
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