Total RNA Purification 96-Well Kit (Cat. 24300, 24350, 24370)

 

Purchasing Information

  • Extract high quality & purity total RNA including miRNA 
  • Convenient high throughput format for vacuum, centrifuge, or robot
  • Very consistent well-to-well RNA isolation
  • No phenol step required - isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Isolate from a wide variety of specimens
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

For rapid high-throughput purification of total RNA including microRNA

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Create your account to receive 15% off on your first purchase!

Total RNA Purification 96-Well Kit (Cat. 24300) 2 x 96-well plates
Total RNA Purification 96-Well Kit (Deep Well Format) (Cat. 24350) 2 x 96-well plates
Total RNA Purification Kit 96 Well Plate Format (Cat. 24370) 6 x 96-well plates
Total RNA Purification 96-Well Kit (Deep Well Format) (Cat. 24380) 6 x 96-well plates

  • Extract high quality & purity total RNA including miRNA 
  • Convenient high throughput format for vacuum, centrifuge, or robot
  • Very consistent well-to-well RNA isolation
  • No phenol step required - isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Isolate from a wide variety of specimens
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

Click for expanded view


Total RNA Purification 96-Well Kit

<p class="rtejustify">This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses. </p>

<div>
<div>
<p class="rtejustify">The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.</p>

<p class="rtejustify">The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.</p>
</div>

<div>&nbsp;</div>
</div>

<p><span style="font-size:16px;color:#B22222;"><strong>Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology</strong></span></p>

<p><iframe allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="" frameborder="0" height="315" src="https://www.youtube.com/embed/bDjn1MOxHRc" width="560"></iframe></p>

Kit Specifications
Maximum Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications
30 minutes
Average Yield
HeLa Cells (1 x 106 Cells)
E. coli (1 x 109 cells)
Brain (10 mg)
Liver (10 mg)
Blood (50 μL, Hamster)
 
15 μg
50 μg
9 μg
26 μg
1 μg
Maximum Amount of Starting Material: 
Whole Blood
Plasma/Serum
Bacteria
Yeast
Fungi
Plant Tissue
 
100 µL
150 µL
1 x 109 cells
1 x 108 cells
40 mg
40 mg
Comparison of Plate Dimensions
Total RNA 96-Well Plates Length (cm) Width (cm) Height (cm)
Cat# 24300 / Cat# 24370: 96-well plate 12.8 8.6 3.4
Cat# 24300 / Cat# 24370: 96-well plate assembled with collection plate 12.8 8.6 4.4
Cat# 24350/ Cat# 24380: 96-well plate (deep well format) 12.8 8.5 3.8
Cat# 24350/ Cat# 24380: 96-well plate (deep well format) assembled with collection plate 12.8 8.6 5.7


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.


Title Molecular diagnosis of SARS-CoV-2 in seminal fluid
Journal Journal of Endocrinological Investigation. 2021.
Authors D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo
Title Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)
Journal Insect Biochemistry and Molecular Biology. 2016.
Authors Fishilevich E, Vélez AM, Khajuria C, Frey ML1, Hamm RL, Wang H, Schulenberg GA, Bowling AJ, Pence HE, Gandra P, Arora K, Storer NP, Narva KE, Siegfried BD
Title Association of novel nonsynonymous single nucleotide polymorphisms in ampD with cephalosporin resistance and phylogenetic variations in ampC, ampR, ompF and ompC in Enterobacter cloacae that are highly resistant to carbapenems
Journal Antimicrobial Agents and Chemotherpy. 2016.
Authors Babouee Flury B, Ellington MJ, Hopkins KL, Turton JF, Doumith M, Loy R, Staves P, Hinic V, Frei R, Woodford N
Title Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis
Journal BMC Genomics. 2015.
Authors Wenli Li, Amy Turner, Praful Aggarwal, Andrea Matter, Erin Storvick, Donna K. Arnett and Ulrich Broeckel
Title Utility of Select Plasma MicroRNA for Disease and Cardiovascular Risk Assessment in Patients with Rheumatoid Arthritis
Journal The Journal of Rheumatology. 2015.
Authors Michelle J. Ormseth, Joseph F. Solus, Kasey C. Vickers, Annette M. Oeser, Paolo Raggi and C. Michael Stein
Title Effects of crude oil exposure and elevated temperature on the liver transcriptome of polar cod (Boreogadus saida)
Journal Aquatic Toxicology. 2015.
Authors Øivind Andersen, Marianne Frantzen, Marte Rosland, Gerrit Timmerhaus, Adrijana Skugor, Aleksei Krasnov
Title Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels.
Journal The EMBO Journal. 2014.
Authors Luca E Lorenzi, Amadou Bah1, Harry Wischnewski, Vadim Shchepachev, Charlotte Soneson, Marco Santagostino and Claus M Azzalin.
Title RNA Expression Profiling of Human iPSC-Derived Cardiomyocytes in a Cardiac Hypertrophy Model.
Journal PLOS One. 2014.
Authors Praful Aggarwal, Amy Turner, Andrea Matter, Steven J. Kattman, Alexander Stoddard, Rachel Lorier, Bradley J. Swanson, Donna K. Arnett, Ulrich Broeckel.
Title Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach.
Journal BMC Microbiology. 2013.
Authors Azizi A, Tang M, Gisonni-Lex L, Mallet L.

Want a discount?

Create your account to receive 15% off on your first purchase!

Full Kits

Total RNA Purification 96-Well Kit (Cat. 24300) 2 x 96-well plates
Total RNA Purification 96-Well Kit (Deep Well Format) (Cat. 24350) 2 x 96-well plates
Total RNA Purification Kit 96 Well Plate Format (Cat. 24370) 6 x 96-well plates
Total RNA Purification 96-Well Kit (Deep Well Format) (Cat. 24380) 6 x 96-well plates

  • Extract high quality & purity total RNA including miRNA 
  • Convenient high throughput format for vacuum, centrifuge, or robot
  • Very consistent well-to-well RNA isolation
  • No phenol step required - isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Isolate from a wide variety of specimens
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

Click for expanded view


Total RNA Purification 96-Well Kit

<p class="rtejustify">This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses. </p>

<div>
<div>
<p class="rtejustify">The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.</p>

<p class="rtejustify">The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.</p>
</div>

<div>&nbsp;</div>
</div>

<p><span style="font-size:16px;color:#B22222;"><strong>Top 5 Reasons to Choose Silicon Carbide RNA Extraction Technology</strong></span></p>

<p><iframe allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen="" frameborder="0" height="315" src="https://www.youtube.com/embed/bDjn1MOxHRc" width="560"></iframe></p>

Kit Specifications
Maximum Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications
30 minutes
Average Yield
HeLa Cells (1 x 106 Cells)
E. coli (1 x 109 cells)
Brain (10 mg)
Liver (10 mg)
Blood (50 μL, Hamster)
 
15 μg
50 μg
9 μg
26 μg
1 μg
Maximum Amount of Starting Material: 
Whole Blood
Plasma/Serum
Bacteria
Yeast
Fungi
Plant Tissue
 
100 µL
150 µL
1 x 109 cells
1 x 108 cells
40 mg
40 mg
Comparison of Plate Dimensions
Total RNA 96-Well Plates Length (cm) Width (cm) Height (cm)
Cat# 24300 / Cat# 24370: 96-well plate 12.8 8.6 3.4
Cat# 24300 / Cat# 24370: 96-well plate assembled with collection plate 12.8 8.6 4.4
Cat# 24350/ Cat# 24380: 96-well plate (deep well format) 12.8 8.5 3.8
Cat# 24350/ Cat# 24380: 96-well plate (deep well format) assembled with collection plate 12.8 8.6 5.7


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.

Title Molecular diagnosis of SARS-CoV-2 in seminal fluid
Journal Journal of Endocrinological Investigation. 2021.
Authors D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo
Title Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)
Journal Insect Biochemistry and Molecular Biology. 2016.
Authors Fishilevich E, Vélez AM, Khajuria C, Frey ML1, Hamm RL, Wang H, Schulenberg GA, Bowling AJ, Pence HE, Gandra P, Arora K, Storer NP, Narva KE, Siegfried BD
Title Association of novel nonsynonymous single nucleotide polymorphisms in ampD with cephalosporin resistance and phylogenetic variations in ampC, ampR, ompF and ompC in Enterobacter cloacae that are highly resistant to carbapenems
Journal Antimicrobial Agents and Chemotherpy. 2016.
Authors Babouee Flury B, Ellington MJ, Hopkins KL, Turton JF, Doumith M, Loy R, Staves P, Hinic V, Frei R, Woodford N
Title Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis
Journal BMC Genomics. 2015.
Authors Wenli Li, Amy Turner, Praful Aggarwal, Andrea Matter, Erin Storvick, Donna K. Arnett and Ulrich Broeckel
Title Utility of Select Plasma MicroRNA for Disease and Cardiovascular Risk Assessment in Patients with Rheumatoid Arthritis
Journal The Journal of Rheumatology. 2015.
Authors Michelle J. Ormseth, Joseph F. Solus, Kasey C. Vickers, Annette M. Oeser, Paolo Raggi and C. Michael Stein
Title Effects of crude oil exposure and elevated temperature on the liver transcriptome of polar cod (Boreogadus saida)
Journal Aquatic Toxicology. 2015.
Authors Øivind Andersen, Marianne Frantzen, Marte Rosland, Gerrit Timmerhaus, Adrijana Skugor, Aleksei Krasnov
Title Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels.
Journal The EMBO Journal. 2014.
Authors Luca E Lorenzi, Amadou Bah1, Harry Wischnewski, Vadim Shchepachev, Charlotte Soneson, Marco Santagostino and Claus M Azzalin.
Title RNA Expression Profiling of Human iPSC-Derived Cardiomyocytes in a Cardiac Hypertrophy Model.
Journal PLOS One. 2014.
Authors Praful Aggarwal, Amy Turner, Andrea Matter, Steven J. Kattman, Alexander Stoddard, Rachel Lorier, Bradley J. Swanson, Donna K. Arnett, Ulrich Broeckel.
Title Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach.
Journal BMC Microbiology. 2013.
Authors Azizi A, Tang M, Gisonni-Lex L, Mallet L.

 

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